Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,762,814	C→T	5.5%	L228L (CTG→CTA)	CJ019_05404 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,762,814	0	C	T	5.5%	242.8 / 3.4	72	L228L (CTG→CTA)	CJ019_05404	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/43);  new base T (2/2);  total (27/45)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

CACAGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCCATCATCAGGTCAC  >  ACB122/5762685‑5762947                                                                                                                                  |                                                                                                                                      cacaGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTc                                                                                                                                  <  2:191050/135‑1 (MQ=255)    aGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGTGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTcgcg                                                                                                                               <  3:286936/135‑1 (MQ=255)    aGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTcgcg                                                                                                                               <  8:279632/135‑1 (MQ=255)    aGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTcgcg                                                                                                                               <  6:219187/135‑1 (MQ=255)     gTCAGGCCCTGCAAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGa                                                                                                                              >  6:76420/1‑135 (MQ=255)        aGGCCCTGCCAGCTCAACTCATCCAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCTCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAAcc                                                                                                                           <  3:16571/135‑1 (MQ=255)           cccTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCa                                                                                                                        >  4:451672/1‑135 (MQ=255)            ccTGCCAGCTCACCTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCAc                                                                                                                       >  5:289229/1‑135 (MQ=255)              tGCCAGCTCAACTCATCAAACAGGTAACGTCCAGCCATCTGTATTTGAAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGTGAGAACACCAGAGTGTCAGTTCGCGCACCTCACt                                                                                                                      >  6:108123/1‑134 (MQ=255)                 cAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTgg                                                                                                                    <  7:73136/133‑1 (MQ=255)                   gCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGc                                                                                                                >  1:34888/1‑135 (MQ=255)                   gCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGc                                                                                                                >  3:352882/1‑135 (MQ=255)                           catcaAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGATGCGGTAATCa                                                                                                        >  8:464830/1‑135 (MQ=255)                              caAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAAGGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGGCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCa                                                                                                     <  4:87193/135‑1 (MQ=255)                               aaaCCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCaa                                                                                                    >  8:13024/1‑135 (MQ=255)                                aaCCGTTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAg                                                                                                   <  6:312386/135‑1 (MQ=255)                                 aacGGTAACGTCCAGCCAGCAGTATTAGCAACCCCGCGCCGAATGCTGCCTGGGCGTCGAAACTCCGGACCACGACTTCTCGACAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCCGTAATCAGCACGc                                                                                                  >  8:407230/3‑135 (MQ=255)                                        aCGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTTAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCaa                                                                                           >  4:353995/1‑135 (MQ=255)                                        aCGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTTAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCa                                                                                            >  4:360239/1‑134 (MQ=255)                                          gTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACa                                                                                         <  1:394134/135‑1 (MQ=255)                                          gTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACa                                                                                         <  4:70593/135‑1 (MQ=255)                                            ccAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGc                                                                                       <  2:212441/135‑1 (MQ=255)                                            ccAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGc                                                                                       <  7:160980/135‑1 (MQ=255)                                            ccAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGc                                                                                       <  4:409528/135‑1 (MQ=255)                                            ccAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGc                                                                                       <  3:451672/135‑1 (MQ=255)                                                ccATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCAt                                                                                   >  1:57750/1‑135 (MQ=255)                                                ccATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCAt                                                                                   >  1:84142/1‑135 (MQ=255)                                                ccATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCAt                                                                                   >  4:230933/1‑135 (MQ=255)                                                   tCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGa                                                                                >  4:436724/1‑135 (MQ=255)                                                       tATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACgt                                                                            >  7:76323/1‑135 (MQ=255)                                                       tATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACgt                                                                            >  7:357912/1‑135 (MQ=255)                                                        atatGCAACGCCGAGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGATACCACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCTGTAATCAGCAATCCGGGCAACAGCTCATGGAACgtg                                                                           <  2:247171/132‑1 (MQ=255)                                                          ttGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACgtgt                                                                          <  8:250317/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  2:460654/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  1:454277/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  1:443113/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  4:36968/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  3:211628/134‑1 (MQ=255)                                                              aaCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCg                                                                     <  7:13024/135‑1 (MQ=255)                                                               aCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTc                                                                                                                                  <  3:294260/73‑1 (MQ=255)                                                               aCGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTCTCAGTTc                                                                                                                                  >  4:294260/1‑73 (MQ=255)                                                                cgccgcgacgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGGCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGcc                                                                   <  4:260342/135‑1 (MQ=255)                                                                   cgcgccgTATGCTGCGTGGGCGTGGAAACTGCGGTCCAGTCCTTAGTGAGAACACCAGAGTGTCTGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCGCTc                                                                <  1:186257/135‑1 (MQ=255)                                                                   cgcgccgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTc                                                                <  3:230933/135‑1 (MQ=255)                                                                   cgcgccgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTc                                                                <  3:461732/135‑1 (MQ=255)                                                                   cgcgccgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTc                                                                <  7:36605/135‑1 (MQ=255)                                                                    tcgccgAATGCTGCCTGGGCGTCGAAACTGCTGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCATCAAGCCGGGCATCAGCTCATGGAACGTGTCTTCGCCCTCa                                                               <  4:428772/134‑1 (MQ=255)                                                                    gcgccgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACGGGAGGCGGTAATCAGCAAGCCGGGCACCAGCTCATGGACCGTGTCTTCGCCCTCa                                                               >  7:242889/1‑135 (MQ=255)                                                                        cgAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTa                                                           <  3:436724/135‑1 (MQ=255)                                                                           aTGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAgg                                                        <  6:269129/135‑1 (MQ=255)                                                                                gcCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTAcc                                                   <  6:289229/135‑1 (MQ=255)                                                                                  cTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCg                                                 <  5:268710/135‑1 (MQ=255)                                                                                  cTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCg                                                 <  7:400186/135‑1 (MQ=255)                                                                                        gTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAg                                           <  8:222023/135‑1 (MQ=255)                                                                                            aaaCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGc                                                                                                                >  3:326193/1‑62 (MQ=255)                                                                                            aaaCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGc                                                                                                                <  4:326193/62‑1 (MQ=255)                                                                                            aaaCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      <  3:117531/104‑1 (MQ=255)                                                                                            aaaCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTc                                                                      >  4:117531/1‑104 (MQ=255)                                                                                             aaCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGca                                      <  8:105084/135‑1 (MQ=255)                                                                                              aCTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGcac                                     >  3:456528/1‑135 (MQ=255)                                                                                                   ggACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGa                                <  4:166436/135‑1 (MQ=255)                                                                                                    gaacAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCTGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCAAg                               <  7:464830/132‑1 (MQ=255)                                                                                                            cTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGc                       <  1:353458/135‑1 (MQ=255)                                                                                                            cTTCGGGAGAACACCAGAGTCTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGc                       <  6:319217/135‑1 (MQ=255)                                                                                                             ttCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCg                      >  5:175091/1‑135 (MQ=255)                                                                                                                     aaCACCAGAGTGTTAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCca              <  3:353995/135‑1 (MQ=255)                                                                                                                     aaCACCAGAGTGTTAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCca              <  3:360239/135‑1 (MQ=255)                                                                                                                       cacCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCcatc            >  8:312326/1‑135 (MQ=255)                                                                                                                       cacCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCcatc            >  8:376947/1‑135 (MQ=255)                                                                                                                        acCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCcatca           >  2:157303/1‑135 (MQ=255)                                                                                                                           agagTGTCAGTTCGAGAACCTAACTGGAGGCTGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTAGCCATCACTTAAGGCTACCCGCACCAGTTGCACATCGAGACATGTGCTCTAGCCCAtcatca        >  4:451006/1‑135 (MQ=255)                                                                                                                                 tCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCCATCATCAGGTCAc  <  3:433407/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      CACAGTCAGGCCCTGCCAGCTCAACTCATCAAACCGGTAACGTCCAGCCATCTGTATTTGCAACGCCGCGCCGAATGCTGCCTGGGCGTCGAAACTGCGGACCAGGCCTTCGGGAGAACACCAGAGTGTCAGTTCGCGAACCTCACTGGAGGCGGTAATCAGCAAGCCGGGCAACAGCTCATGGAACGTGTCTTCGCCCTCACGTAAGGCTACCCGCACCAGTTGCACATCGAGCCCGGTGCGGTTGCCCATCATCAGGTCAC  >  ACB122/5762685‑5762947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑