Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,798,711	A→C	18.1%	N215T (AAC→ACC)	mrcA →	Penicillin‑binding protein 1A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,798,711	0	A	C	18.1%	90.5 / 2.4	56	N215T (AAC→ACC)	mrcA	Penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  ref base A (38/6);  new base C (4/7);  total (43/13)
Fisher's exact test for biased strand distribution p-value = 1.73e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGCCGACGCC  >  ACB122/5798580‑5798840                                                                                                                                    |                                                                                                                                    cAACCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAAccc                                                                                                                                  <  8:375447/135‑1 (MQ=255)  aaCCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCg                                                                                                                                 >  4:7996/1‑135 (MQ=255)  aaCCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCg                                                                                                                                 >  1:303259/1‑135 (MQ=255)   aCCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCCAGGCACCGTCGCGCTTCAACCCGCTGGCCACCCCgg                                                                                                                                >  2:382780/1‑135 (MQ=255)       cgcCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTg‑cg                                                                                                                           >  3:228956/1‑135 (MQ=255)         ccTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTg‑cgcg                                                                                                                         <  3:152451/135‑1 (MQ=255)                 aTCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAagag                                                                                                                 >  3:202934/1‑135 (MQ=255)                     aTGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCACCCCGGTG‑CGCGCCAAAGAGcg‑cc                                                                                                            >  4:154740/1‑135 (MQ=255)                     aTGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGcg‑cc                                                                                                            >  1:263116/1‑135 (MQ=255)                        cagcagcaCAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCTCGCTTCAACCCGCTGGCCAACCCCGTG‑CGCGCCAAAGAGCG‑Ccgcg                                                                                                         >  5:194478/1‑135 (MQ=255)                           cagcaCAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACACGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACt                                                                                                      >  4:333743/1‑135 (MQ=255)                             gcaCAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTgg                                                                                                    >  1:43358/1‑135 (MQ=255)                             gcaCAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTgg                                                                                                    >  5:79189/1‑135 (MQ=255)                                   gtgtATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTc                                                                                              >  1:258271/1‑135 (MQ=255)                                   gtgtATTACGGCAAGTCCATCCGTGACGTGAGACTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCCACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTc                                                                                              >  2:2894/1‑135 (MQ=255)                                     ttATTACGGCAAGGCCATCCGGGAGGGGCGCCTGGCGCTGTGGGCGATGAGCGCGGGCCTGCCCACGGCACCGGCGCGCTTCAACCCGCTGGCCACCCCGGGG‑CGCGCAAAAGCGCC‑CCGCGACTGGATCCTCgg                                                                                            <  2:303259/134‑1 (MQ=255)                                      tATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCCCCCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCggg                                                                                           >  4:259333/1‑135 (MQ=255)                                      tATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCCACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCGCggg                                                                                           >  6:361641/1‑135 (MQ=255)                                      tATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCggg                                                                                           >  2:399567/1‑135 (MQ=255)                                           cGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCCACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGCCGGGATCCTCGGGCGCAt                                                                                      >  2:220805/1‑135 (MQ=255)                                           cGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAGCCCCGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCAt                                                                                      >  3:430945/1‑135 (MQ=255)                                           cGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCAt                                                                                      >  3:383548/1‑135 (MQ=255)                                           cGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCAt                                                                                      >  7:449665/1‑135 (MQ=255)                                           cGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCAt                                                                                      >  5:218995/1‑135 (MQ=255)                                              cAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCCACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGCTCCTCGGGCGCATgga                                                                                   >  5:280072/1‑133 (MQ=255)                                              cAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGt                                                                                    >  2:337900/1‑134 (MQ=255)                                                aGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCCACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGCTCCTCGGGCGCATGTa                                                                                   >  7:288964/1‑133 (MQ=255)                                                aGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGCTCCTCGGGCGCATGTa                                                                                   >  8:291221/1‑133 (MQ=255)                                                aGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTa                                                                                   >  3:99273/1‑133 (MQ=255)                                                aGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTa                                                                                   >  5:449731/1‑133 (MQ=255)                                                aGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTa                                                                                   >  3:200548/1‑133 (MQ=255)                                                   ccATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGCCTGGATCCTCGGGCGCATGTACAAGc                                                                              >  5:452520/1‑135 (MQ=255)                                                   ccATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGc                                                                              >  6:316369/1‑135 (MQ=255)                                                   ccATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACAGGAACCAACGGCGCATGTACAAGc                                                                              >  6:358530/1‑135 (MQ=255)                                                   ccATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCAGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCAAGTACAAga                                                                              >  7:161465/1‑134 (MQ=255)                                                     aTCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTg                                                                            >  6:62892/1‑135 (MQ=255)                                                         gtgacgtgaGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTggg                                                                          >  1:2870/1‑133 (MQ=255)                                                              gtgaGCCTGGCGAAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTACAACCCGCTGGCCACCCCCGAG‑CGCGCCAAAGACCG‑CCCCCACTGGATCCTCGCGCGCCTGTACAAGCTGGGCAAGAt                                                                    >  7:2041/1‑134 (MQ=255)                                                               tgaGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCg                                                                  >  8:276117/1‑135 (MQ=255)                                                                    cTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAg                                                             >  1:273714/1‑135 (MQ=255)                                                                              tGGCGATCAGCGCCGGCCCGCCCCAGCCACCGGCGCGCCTCACCCCGCGGCCCCCCCCGGGC‑CGCGCCAAAGCGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACg                                                   <  4:86749/135‑1 (MQ=255)                                                                                       gccccGGCCGCCCCACGCCCCCGGCGCGCCCCACCCCGC‑GGCCCACCCGGGGCCGCCCCAACCCCCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGccc                                          <  7:104790/132‑1 (MQ=255)                                                                                                     aaGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTc                            >  3:173262/1‑135 (MQ=255)                                                                                                       gccACCGGGGCGCTTCCCCCCGCGGCCCACCCCGGGG‑CGCCCAACCGCGCC‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCaa                          <  3:120677/133‑1 (MQ=255)                                                                                                          cccGGCGCGCTCCCCCCCGCGGGCCACCCCGGGC‑CGCGCCAAAGCGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGc                       <  7:7910/134‑1 (MQ=255)                                                                                                               cgcgCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGCCTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGCGCCGCTCACCGCCAGCt                  >  1:295207/1‑135 (MQ=255)                                                                                                               cgcgCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGCGCCGCTCAACGCCAGCt                  >  5:433912/1‑135 (MQ=255)                                                                                                                gcgcTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTa                 >  3:287021/1‑135 (MQ=255)                                                                                                                      cAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGt           >  6:392102/1‑135 (MQ=255)                                                                                                                       accccGCGGCCCACCCGGGTG‑CGCGCCAACGCGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGt           <  1:283241/132‑1 (MQ=255)                                                                                                                       aaCCCGGTGCCCACCCCGGGG‑CGCGCCACCCCCCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGt           <  3:454344/134‑1 (MQ=255)                                                                                                                       aaCCCGCGGCCCAACCCGGGG‑CGCGCCAACCCCCC‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGt           <  3:446985/134‑1 (MQ=255)                                                                                                                        ccccGCTGCCCCCCCCGGGG‑CGCCCCAACGCGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGc         <  7:336882/134‑1 (MQ=255)                                                                                                                         cccgggggCCCACCCGGGG‑CGCCCCAAACCGCGCCCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGc         <  2:258271/129‑1 (MQ=255)                                                                                                                           cGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGCCgc      >  1:154351/1‑134 (MQ=255)                                                                                                                               gcccAACCCGGGG‑CGCCCAAAAGCGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGCCGACGcc  <  6:187524/133‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CAACCGCGCCTACGGTATCGATGCAGCAGCACAGGTGTATTACGGCAAGTCCATCCGTGACGTGAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCCAAGGCACCGTCGCGCTTCAACCCGCTGGCCAACCCGGTG‑CGCGCCAAAGAGCG‑CCGCGACTGGATCCTCGGGCGCATGTACAAGCTGGGCAAGATCGACCAGGCCAGCTACGAGGCCGCCCTGGCCGAGCCGCTCAACGCCAGCTATCACGTGCCGACGCC  >  ACB122/5798580‑5798840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑