Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,921,203 G→A 8.2% L168L (CTG→TTG)  CJ019_05543 ← putative binding protein component of ABC iron transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,921,2030GA 8.2% 227.8 / 6.5 73L168L (CTG→TTG) CJ019_05543putative binding protein component of ABC iron transporter
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base A (3/3);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGGCGC  >  ACB122/5921070‑5921334
                                                                                                                                     |                                                                                                                                   
cttggCCCGGCGTCGATGGCCGGGATCACCGCGTTGTCGGGGGTGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCGCCGGCTTGGCCTCACCTGGGGTTTCGAGCAGGGTGGCGGGCAGc                                                                                                                                    <  4:58496/131‑1 (MQ=255)
catTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGGCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGc                                                                                                                                    <  7:167405/135‑1 (MQ=255)
catTGCCCGGCCTCGATGGCCTGGAGCCCCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGc                                                                                                                                    <  4:289472/135‑1 (MQ=255)
   tGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGAc                                                                                                                                 <  6:156189/135‑1 (MQ=255)
   tGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTGTCGATCAGGGTGGCGGTCAGCgcc                                                                                                                                 >  2:339646/1‑133 (MQ=255)
      ccGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTgg                                                                                                                              >  4:199442/1‑135 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  8:24709/135‑1 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  3:424392/135‑1 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  2:104264/135‑1 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGGTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           >  7:320086/1‑135 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGGGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  8:155275/135‑1 (MQ=255)
         gCCTCGATGGCCTGGATCACCGCGTTGTCGGCGGAGAACACATCGGTGGACTGGTTGTTGACCCACCCTTTGACTATTTGGTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGTTCAGCGACTGGTTg                                                                                                                           <  4:330329/135‑1 (MQ=255)
         gCCTCGATGGCCTGGAGCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCCCCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  2:458798/135‑1 (MQ=255)
         gCCTCGATGGCCGGGATCACCGCGTTGTCGGCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTg                                                                                                                           <  2:393838/135‑1 (MQ=255)
           cTCGATGGCCTGGATCCCCGCGTTGTCGGCGGAGAACACCTCGGTGGACAGGTTGTTGACCCACCCTTTGACGAGTGGCTAGGTCTTGGCCTCCCCGGGGGTTTCGCGCAGGGGGGCGGTCAGCGCCGGGTTgga                                                                                                                         >  2:124831/1‑133 (MQ=255)
           cTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTa                                                                                                                         >  5:33045/1‑135 (MQ=255)
             cGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTaca                                                                                                                       >  2:203229/1‑135 (MQ=255)
                       gATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACACACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGTTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGc                                                                                                             >  6:278459/1‑135 (MQ=255)
                               cgTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCg                                                                                                                                   <  1:390905/105‑1 (MQ=255)
                               cgTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCg                                                                                                                                   >  2:390905/1‑105 (MQ=255)
                               cgTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCa                                                                                                     >  1:434185/1‑135 (MQ=255)
                                gTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACACTCCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGAGCAGGGTGGCGGTCAGCGACTGGTTGTACACCTGCTTGGCGGTgcgcgg                                                                                                    >  1:92222/1‑133 (MQ=255)
                                  tGTCGTCGGAGAACACATCGGTTGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTACACCTTCTTGGCAGTGCGCAGGc                                                                                                  >  1:277850/1‑135 (MQ=255)
                                  tGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTAGACCTTCTTGGCCGTGCGCAGGc                                                                                                  >  1:284901/1‑135 (MQ=255)
                                  tGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTGTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGc                                                                                                  >  1:277851/1‑135 (MQ=255)
                                    tcgtcgGAGAACACATCGGGGGACAGGTTGGTGACCCACACTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGca                                                                                                 <  6:431043/134‑1 (MQ=255)
                                     cgtcgGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGcaca                                                                                               <  1:287706/135‑1 (MQ=255)
                                     cgtcgGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGGGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGcaca                                                                                               <  5:134811/135‑1 (MQ=255)
                                        cgGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGAc                                                                                            <  8:320086/135‑1 (MQ=255)
                                        cgGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGAc                                                                                            <  5:455045/135‑1 (MQ=255)
                                                      gTGGTCAGGTTGTTGACCCACGCTTTGAGGATTTGCTCGGTGTTGGTCTCTCGGTGGGTGTGGTTCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTGTTGGCCGTGGGCAGGCACAGACGGCCTTCCCACTGt                                                                              <  3:131348/135‑1 (MQ=255)
                                                            aGGTTCATGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACAGGTTGTACACATACTAGGACGTGAGCAGGCACAGACGGCATTCCCACTGTAAGTCa                                                                        >  2:376741/1‑135 (MQ=255)
                                                               ttgttgACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGc                                                                      <  2:434185/134‑1 (MQ=255)
                                                                 gttgACCCGCCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAg                                                                   >  7:76480/1‑135 (MQ=255)
                                                                 gttgACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAg                                                                   >  3:224012/1‑135 (MQ=255)
                                                                 gttgACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAg                                                                   >  4:181802/1‑135 (MQ=255)
                                                                       ccacccTTTGACGATTTGCTCGTTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACACACGGCCTTCCCACTGTTTGACAGCCAGGCCTTc                                                             >  2:147744/1‑135 (MQ=255)
                                                                             tttGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGt                                                       >  4:344044/1‑135 (MQ=255)
                                                                                        gCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAAct                                               <  3:327998/132‑1 (MQ=255)
                                                                                        gCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAAct                                               >  4:327998/1‑132 (MQ=255)
                                                                                        gCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGAACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAActcct                                            >  4:253187/1‑135 (MQ=255)
                                                                                             gTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGt                                       <  6:184682/135‑1 (MQ=255)
                                                                                                   gCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAc                                  <  7:453793/134‑1 (MQ=255)
                                                                                                    ccTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAccc                                <  3:199442/135‑1 (MQ=255)
                                                                                                    ccTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGTGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAccc                                <  2:277850/135‑1 (MQ=255)
                                                                                                    ccTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAccc                                <  2:284901/135‑1 (MQ=255)
                                                                                                    ccTCACCGTGGGTTTCGATCAGGGTGGCGGTCAACGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGAccc                                <  2:277851/135‑1 (MQ=255)
                                                                                                         ccGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCg                           <  5:399607/135‑1 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGCCGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  4:233187/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGCCCCGCTCGGt                         >  6:415175/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCt                             >  7:51538/1‑131 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCt                             <  8:51538/131‑1 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         <  4:375113/135‑1 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  8:50812/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  1:51877/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  2:248402/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  3:336222/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         >  3:38096/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCAGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTTCTAAACTCCTCGGGTTTGACCCGCTCGGt                         >  8:432494/1‑135 (MQ=255)
                                                                                                           gTGGGTTTCGAGCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGt                         <  4:375115/135‑1 (MQ=255)
                                                                                                                tttCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGa                      >  8:1039/1‑133 (MQ=255)
                                                                                                                    gATCAGGGTGGCGGTCAGCGACTGGGTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAga                 <  4:244463/134‑1 (MQ=255)
                                                                                                                     aTCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGACAGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAgatg               >  6:79376/1‑135 (MQ=255)
                                                                                                                      tCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGttt                                     <  4:150190/112‑1 (MQ=255)
                                                                                                                      tCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGttt                                     >  3:150190/1‑112 (MQ=255)
                                                                                                                             ggCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCg       >  4:295789/1‑135 (MQ=255)
                                                                                                                                gggcAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc    <  1:197747/132‑1 (MQ=255)
                                                                                                                                ggTCTGCGAGTGTTTGTACAGGTTGTGGGGGGTGCGAGGGCACAGACGGCGTTCCCAGTGTTTGTCAGCCTGGGATGCGTTGTTGCTAAAGTGGTCGGGTTTGCCGCGCTCGGTGGAGTAGATGATGGTGCGGgc    <  2:218205/135‑1 (MQ=255)
                                                                                                                                ggTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc    <  1:324704/135‑1 (MQ=255)
                                                                                                                                ggTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCGCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgc    <  2:345325/135‑1 (MQ=255)
                                                                                                                                 gTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTTGATGATGGTGCGGgcg   <  1:339646/135‑1 (MQ=255)
                                                                                                                                  tCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGgcgc  >  8:328960/1‑135 (MQ=255)
                                                                                                                                   cAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTATGTGCTCAACTCCTCTGGTTTGACCCGCTCGGTGGAGTAGATGATGGggcgggcg   <  2:118207/133‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CATTGCCCGGCCTCGATGGCCTGGATCACCGCGTTGTCGTCGGAGAACACATCGGTGGACAGGTTGTTGACCCACCCTTTGACGATTTGCTCGGTCTTGGCCTCACCGTGGGTTTCGATCAGGGTGGCGGTCAGCGACTGGTTGTACACCTTCTTGGCCGTGCGCAGGCACAGACGGCCTTCCCACTGTTTGTCAGCCAGGGCTTCGTAGGTGCTCAACTCCTCGGGTTTGACCCGCTCGGTGGAGTAGATGATGGTGCGGGCGC  >  ACB122/5921070‑5921334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: