| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,974,074 | A→C | 63.9% | Q688P (CAG→CCG) | sasA_19 → | Adaptive‑response sensory‑kinase SasA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,974,074 | 0 | A | C | 63.9% | ‑4.7 / 6.2 | 25 | Q688P (CAG→CCG) | sasA_19 | Adaptive‑response sensory‑kinase SasA |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (11/4); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01 | |||||||||||
TGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGCCATGC‑GCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC > ACB122/5973946‑5974206 | tGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCCGGC‑GCCCGGCTTg > 7:285786/1‑135 (MQ=255) cAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCCGGGCATGC‑GCCCGGGGTGGCCCGGGGGCACGCCCgcg > 7:132521/1‑133 (MQ=255) gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCCTGC‑GCCCGGCATGGCCAGGGCGCACGCCCCAGGggttggtgtg > 3:123162/1‑130 (MQ=255) cATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCAATC‑GCCCGGCATGGCCCGGGCGCACGCACGACGGGTTGTTGGGGCTCgccacc > 1:45409/1‑133 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGCGCCCTGC‑GCCCGGCGTGGGCCCGGCGCAAGCCCCAGGGGGTGTTTGCGCGCGCCCGCCCGCGTCAAcc > 1:82158/1‑134 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCCAGC‑GCCCCGCGTGGGCACGGCGCCCGCCCGAGGGGGTTTTTGCGCTCGCCCCCCACCTACaccc > 2:369795/1‑132 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCATGC‑GCCAGGCTTGGGCAGGGCGCACGCACCACGTGTTGTTAGAGCTCGCCAGCCAGCTACAAcg > 8:215565/1‑135 (MQ=255) aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGCCCAGC‑GCCCCGCGTGGGCCGGGGGGACGGACGAGGGGGTGTTTGCGCTCGCGAGCCCGCTAAAAccc > 4:253297/1‑133 (MQ=255) aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCCGGCCCAGC‑GGCCGGCGTGGGCCCGGCGGACCCCCCCGGTGTTGTTTGGGCTGGCCCGCCCCCGACAAccc > 1:241674/1‑133 (MQ=255) ggggggcggcTTATCCACCCCCCCCGGGGCACCTCCGCGCTGCGCTGCAGGCA‑GTAATCCCCCTGGCCATGC‑GCGCGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg < 4:109000/131‑1 (MQ=255) ggAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCAGGCA‑CGTATCGCCCTGGCCAGGC‑GCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg < 8:380497/134‑1 (MQ=255) cGCTTAGGCACTCACGCCCGTGCACCCGCGCGCCTCGCGGCATGCA‑CGTATCGCCCTGGCCATGC‑GCCCGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGccc < 2:45409/135‑1 (MQ=255) cTTATGCACTCCCGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGCCCTGC‑GGCCCGCCTGGGCACGGCGGACGGCGCCGGGGGTGTTCGCGCTCGCCCGCCCGCGCAAAAGCGCCCGccccg > 8:293620/1‑134 (MQ=255) cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCCCTTC‑GCCACGCTTGGCCAGGGCGCACGCAACATGTGGTGTTAGAGCTAGCCAGCCCGCGTCAACGCGCCCGCCCCt > 8:282149/1‑135 (MQ=255) cACTCCCGCCGGTGCACCAGCGCGCCTGGC‑GCCTGGACCGTATCGCCGTGGCCATCC‑GCATGGGTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGt < 1:306076/135‑1 (MQ=255) cccGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGCCCTGC‑GCCCGGGGTGGGCCGGGCGCACGCCCGAGGGGGTGGTAGAGGTCGCCCGCCCGCGGCCACCCGCCCGCCCCGGCGGGGTTggctcg > 4:32526/1‑130 (MQ=255) cccGTGCACCAGCGCGCCTCGCGGCATGCA‑CGTATCGCCCTGGCCATGC‑GCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCg < 3:206648/135‑1 (MQ=255) aCCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGGCAGGCGGCCGGGGTTGGCC‑GGGCGGTCGCCGCCGGTGGGGTTGGCGCTCGCCCACCCGCGGCAACACGCCCGCCCCCGCGGGTTTGTCGCGGGTgcca > 8:253559/1‑132 (MQ=255) aCCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCCGGCCCAGC‑GCCCGGCCTGGGCCGGGGGCCCGCACGCGGGGGTGGTGGGGCGCGGCCGCCAGCGTCCCCCCGCCCGCCCCGGCGGGGTTGCAGCggggggcg > 6:393713/1‑129 (MQ=255) ggCTGCAGGCA‑GGTAGCGCCGTGGCCATGC‑GCCAGGCTTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTcc < 7:85694/134‑1 (MQ=255) a‑CGTCTCGCCCTGGCCAGGC‑GCCCGGCTGGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCtttt < 6:351930/135‑1 (MQ=255) a‑CGTAGCGCCCGGCCCAGCC‑GCCCGGCTGGCCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCtttt < 1:320942/135‑1 (MQ=255) gTAGCGCCCTGCCCATGC‑GCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTAc < 1:54714/135‑1 (MQ=255) tGGCCATGC‑GCCTGGCTTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCg < 6:241069/135‑1 (MQ=255) ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTCTTACGCGAAGCCGCATCATgcgc > 7:280058/1‑135 (MQ=255) | TGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGCCATGC‑GCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC > ACB122/5973946‑5974206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |