Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,020,335 T→G 39.7% S60R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,020,3350TG39.7% 34.6 / 8.2 50S60R (AGC→CGC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (11/17);  new base G (12/9);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCA  >  ACB122/6020201‑6020460
                                                                                                                                      |                                                                                                                             
cATGCCGATCATCAGCCGCAGCAGCAGCCATAACCGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                               <  5:341628/135‑1 (MQ=255)
cATGCCGATCAGCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                               <  8:440217/135‑1 (MQ=255)
cATGCCGAGCATCAGCCGCAGCAGCCGCCATAACGGGTACCTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                               <  3:457957/135‑1 (MQ=255)
    ccGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCACCCTGCCAGGCGGCGGGcc                                                                                                                           >  3:253519/1‑135 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCACTCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGccc                                                                                                                          >  4:314577/1‑134 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCGGGCCa                                                                                                                          >  7:178524/1‑135 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGccc                                                                                                                          >  1:26419/1‑134 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGGACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                          <  8:112440/135‑1 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGcccg                                                                                                                         >  2:377723/1‑133 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGcccc                                                                                                                         >  7:266161/1‑133 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGcccg                                                                                                                         >  3:199381/1‑133 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGcccc                                                                                                                         >  4:15208/1‑133 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAg                                                                                                                         >  3:9215/1‑135 (MQ=255)
          atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTg                                                                                                                     >  7:60530/1‑135 (MQ=255)
          atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGcgg                                                                                                                     <  2:315835/135‑3 (MQ=255)
          atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                     <  2:143537/135‑1 (MQ=255)
              gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGgcgc                                                                                                                 <  5:146651/135‑1 (MQ=255)
                 gcagcagcaGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCACGCTGGCGCCgg                                                                                                              >  2:318862/1‑135 (MQ=255)
                       gcagcCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCCGGCAGGcgcccgccccga                                                                                                        >  6:133637/1‑131 (MQ=255)
                             aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCCGGCACGACGCCCa                                                                                                  >  4:443825/1‑135 (MQ=255)
                              tAACGGGTAACTAGGCAGCAGGGCGTGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCAGCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCaa                                                                                                 <  8:50868/135‑1 (MQ=255)
                                        ctcggCCGCCGCGCGAGCAGGCCAATCGCCACCGCCCCCCCCCACCGGCACCGGCGCAGCGCCGAAGGTACGCCAACCCACCCTGCACGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCAtt                                                                                       <  3:314577/132‑1 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                          >  6:221135/1‑100 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                          <  5:221135/100‑1 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCAGGACCCCCCACCCCCGccat                                                                                       >  3:82028/1‑132 (MQ=255)
                                         tAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGGCGCCGGCCCGGACGCCCAACGCCgccctgt                                                                                      >  5:219758/1‑129 (MQ=255)
                                                cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCAGTACGCCCAACGCCCGCCTTgccgccct                                                                               >  4:104872/1‑133 (MQ=255)
                                                 agcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCGGCCCGGACGCCCCACCCCCGCAGTGCCGCCCTc                                                                              >  4:448267/1‑135 (MQ=255)
                                                             ccAAGCGACAGCGCCCCGCCCCACAGGCACCGGCGCATCGCCGATGGTACGCCAACCCAACCTGCCAGGGGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                  <  6:219758/135‑1 (MQ=255)
                                                               aaTCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCCGCCCCTACGCCCAACGCCCGCCTTGCCGCCCTCACCCCGATGGCGaa                                                                >  7:365851/1‑135 (MQ=255)
                                                                           cccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCGGGCCGGCCGCCCCCCCCCGGCAGTGCCGCCCGCACGCCGCTGGCGCAGCGGCCCGAGcc                                                    >  3:203525/1‑135 (MQ=255)
                                                                           cccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCGGGCCGTTCGCCCCACCCCGGCATTTCCGCCCTCCCGCCCCAGGGGCAGCTGGCGGAGcc                                                    >  3:278349/1‑135 (MQ=255)
                                                                             cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCCGTACGCCCCACGCCCGCCTTGCCGCCCGCACGCCCCTGGCGCAGCTGCCGGAGcccc                                                  >  3:133513/1‑135 (MQ=255)
                                                                                      gccacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCa                                         <  7:70853/133‑1 (MQ=255)
                                                                                       gcacaggcGAACCGCCCAAGGAACGCCAACCCACCCTGCCCGGCGGCTGGCCCGGCTGGCGCCGGCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAg                                        <  3:183484/135‑1 (MQ=255)
                                                                                        cacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAgg                                       <  7:53388/135‑1 (MQ=255)
                                                                                         acaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgc                                      <  8:60530/135‑1 (MQ=255)
                                                                                            ggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCGGGCGCCGGCCAGTACGCCCCCCCCCCCCCTTTCCGCCCACACCCCCCAGTCGAAGCTGCCGGAGCCCCCCGCCGCCACgcgcc                                   >  4:324082/1‑134 (MQ=255)
                                                                                             gcgcATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCCACCCCCGCATTGACGCCAAAACCCCGATTGCGAACCTGCCGTATCCCCAGGCCTCCACgcgccc                                  >  8:140811/1‑133 (MQ=255)
                                                                                                  tCGCCGAAGGAACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGaca                             <  1:377723/135‑1 (MQ=255)
                                                                                                      cGAAGGTACGCCAACCCACCCTCCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                         <  4:396515/135‑1 (MQ=255)
                                                                                                       gAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGc                        <  4:82028/135‑1 (MQ=255)
                                                                                                              cGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGc                 <  1:117080/135‑1 (MQ=255)
                                                                                                              cGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGc                 <  3:109839/135‑1 (MQ=255)
                                                                                                                  accccacccTGCATGGGGGCGGGCCCGGCTGGCGCCGGCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCAc             <  4:341159/133‑1 (MQ=255)
                                                                                                                   acccaaccTGCCCGCCGGCGGGCCAGGGTGGCGCCGGCAAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACg            <  3:439078/135‑1 (MQ=255)
                                                                                                                        cccggCCCGGCGGCGGGCCCGGCGGGCGCCGGCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  1:318862/131‑1 (MQ=255)
                                                                                                                        cccTGCCAGGCGGCTGGCCAGGCTGGCGCCGCCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  1:123849/134‑1 (MQ=255)
                                                                                                                        accTGCCCGCCGGCTGGCCAGGCTGGCGCCGGCCTGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  6:218201/135‑1 (MQ=255)
                                                                                                                        accTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  7:387811/135‑1 (MQ=255)
                                                                                                                        accTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  8:435846/135‑1 (MQ=255)
                                                                                                                        accTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg       <  2:300496/135‑1 (MQ=255)
                                                                                                                             ccAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa  <  4:66929/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                             
CATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCA  >  ACB122/6020201‑6020460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑