Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,020,340 A→C 35.1% L58R (CTG→CGG)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,020,3400AC35.1% 35.9 / 4.9 40L58R (CTG→CGG) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base A (4/21);  new base C (11/4);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 5.37e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020206‑6020474
                                                                                                                                      |                                                                                                                                      
cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCGGGCCa                                                                                                                                        >  7:178524/1‑135 (MQ=255)
cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGGACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                        <  8:112440/135‑1 (MQ=255)
 gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAg                                                                                                                                       >  3:9215/1‑135 (MQ=255)
     atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTg                                                                                                                                   >  7:60530/1‑135 (MQ=255)
     atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGcgg                                                                                                                                   <  2:315835/135‑3 (MQ=255)
     atcaGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTg                                                                                                                                   <  2:143537/135‑1 (MQ=255)
         gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGgcgc                                                                                                                               <  5:146651/135‑1 (MQ=255)
            gcagcagcaGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCACGCTGGCGCCgg                                                                                                                            >  2:318862/1‑135 (MQ=255)
                  gcagcCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCCGGCAGGcgcccgccccga                                                                                                                      >  6:133637/1‑131 (MQ=255)
                        aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCCGGCACGACGCCCa                                                                                                                >  4:443825/1‑135 (MQ=255)
                         tAACGGGTAACTAGGCAGCAGGGCGTGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCAGCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCaa                                                                                                               <  8:50868/135‑1 (MQ=255)
                                   ctcggCCGCCGCGCGAGCAGGCCAATCGCCACCGCCCCCCCCCACCGGCACCGGCGCAGCGCCGAAGGTACGCCAACCCACCCTGCACGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCAtt                                                                                                     <  3:314577/132‑1 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                        >  6:221135/1‑100 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCa                                                                                                                                        <  5:221135/100‑1 (MQ=255)
                                   cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCAGGACCCCCCACCCCCGccat                                                                                                     >  3:82028/1‑132 (MQ=255)
                                    tAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGGCGCCGGCCCGGACGCCCAACGCCgccctgt                                                                                                    >  5:219758/1‑129 (MQ=255)
                                           cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCAGTACGCCCAACGCCCGCCTTgccgccct                                                                                             >  4:104872/1‑133 (MQ=255)
                                            agcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCGGCCCGGACGCCCCACCCCCGCAGTGCCGCCCTc                                                                                            >  4:448267/1‑135 (MQ=255)
                                                        ccAAGCGACAGCGCCCCGCCCCACAGGCACCGGCGCATCGCCGATGGTACGCCAACCCAACCTGCCAGGGGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                                <  6:219758/135‑1 (MQ=255)
                                                          aaTCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCCGCCCCTACGCCCAACGCCCGCCTTGCCGCCCTCACCCCGATGGCGaa                                                                              >  7:365851/1‑135 (MQ=255)
                                                                      cccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCGGGCCGGCCGCCCCCCCCCGGCAGTGCCGCCCGCACGCCGCTGGCGCAGCGGCCCGAGcc                                                                  >  3:203525/1‑135 (MQ=255)
                                                                      cccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCGGGCCGTTCGCCCCACCCCGGCATTTCCGCCCTCCCGCCCCAGGGGCAGCTGGCGGAGcc                                                                  >  3:278349/1‑135 (MQ=255)
                                                                        cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCCGTACGCCCCACGCCCGCCTTGCCGCCCGCACGCCCCTGGCGCAGCTGCCGGAGcccc                                                                >  3:133513/1‑135 (MQ=255)
                                                                                 gccacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCa                                                       <  7:70853/133‑1 (MQ=255)
                                                                                  gcacaggcGAACCGCCCAAGGAACGCCAACCCACCCTGCCCGGCGGCTGGCCCGGCTGGCGCCGGCCAGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAg                                                      <  3:183484/135‑1 (MQ=255)
                                                                                   cacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAgg                                                     <  7:53388/135‑1 (MQ=255)
                                                                                    acaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgc                                                    <  8:60530/135‑1 (MQ=255)
                                                                                       ggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCGGGCGCCGGCCAGTACGCCCCCCCCCCCCCTTTCCGCCCACACCCCCCAGTCGAAGCTGCCGGAGCCCCCCGCCGCCACgcgcc                                                 >  4:324082/1‑134 (MQ=255)
                                                                                        gcgcATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCCACCCCCGCATTGACGCCAAAACCCCGATTGCGAACCTGCCGTATCCCCAGGCCTCCACgcgccc                                                >  8:140811/1‑133 (MQ=255)
                                                                                             tCGCCGAAGGAACGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGaca                                           <  1:377723/135‑1 (MQ=255)
                                                                                                 cGAAGGTACGCCAACCCACCCTCCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                       <  4:396515/135‑1 (MQ=255)
                                                                                                  gAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGc                                      <  4:82028/135‑1 (MQ=255)
                                                                                                         cGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGc                               <  1:117080/135‑1 (MQ=255)
                                                                                                         cGCCAACCCAACCTGCCAGGCGGCGGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGc                               <  3:109839/135‑1 (MQ=255)
                                                                                                             accccacccTGCATGGGGGCGGGCCCGGCTGGCGCCGGCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCAc                           <  4:341159/133‑1 (MQ=255)
                                                                                                              acccaaccTGCCCGCCGGCGGGCCAGGGTGGCGCCGGCAAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACg                          <  3:439078/135‑1 (MQ=255)
                                                                                                                   cccggCCCGGCGGCGGGCCCGGCGGGCGCCGGCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  1:318862/131‑1 (MQ=255)
                                                                                                                   cccTGCCAGGCGGCTGGCCAGGCTGGCGCCGCCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  1:123849/134‑1 (MQ=255)
                                                                                                                   accTGCCCGCCGGCTGGCCAGGCTGGCGCCGGCCTGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  6:218201/135‑1 (MQ=255)
                                                                                                                   accTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  7:387811/135‑1 (MQ=255)
                                                                                                                   accTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  8:435846/135‑1 (MQ=255)
                                                                                                                   accTGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  2:300496/135‑1 (MQ=255)
                                                                                                                        ccAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa                <  4:66929/135‑1 (MQ=255)
                                                                                                                             cgcggggCCCGGCGGGCGCCGCCCCTTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg           <  2:408090/130‑1 (MQ=255)
                                                                                                                                      aGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  4:133513/135‑1 (MQ=255)
                                                                                                                                      aGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  1:23904/135‑1 (MQ=255)
                                                                                                                                      aGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  3:146290/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020206‑6020474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑