Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,048,901 C→G 6.5% I124M (ATC→ATG oxyR → Hydrogen peroxide‑inducible genes activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,048,9010CG 6.5% 284.5 / 8.5 89I124M (ATC→ATGoxyRHydrogen peroxide‑inducible genes activator
Reads supporting (aligned to +/- strand):  ref base C (37/46);  new base G (4/2);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

AGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGATGAACCGTTCTGCGC  >  ACB122/6048768‑6049029
                                                                                                                                     |                                                                                                                                
aGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCg                                                                                                                                 <  3:324051/135‑1 (MQ=255)
  cTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaa                                                                                                                               <  1:189452/135‑1 (MQ=255)
   tGGCCCAGGCCGGCAAGAATCATCTGACCGCTCCGCTCAAGGTCGCCTCCATCTACACCGTAGGCCCCTACCTGTTCCAGAACCAGATCACGCAGCTCCACCGCGAGCCGCCGCAAATGCAGCTGTAAATCgaa                                                                                                                               >  7:152462/1‑134 (MQ=255)
   tGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaa                                                                                                                               >  6:422720/1‑134 (MQ=255)
      cccAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaagaa                                                                                                                            <  3:228751/134‑1 (MQ=255)
       ccAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGTCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          <  1:224854/135‑1 (MQ=255)
       ccAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          <  3:434584/135‑1 (MQ=255)
       ccAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          <  7:207599/135‑1 (MQ=255)
       ccAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          <  1:19162/135‑1 (MQ=255)
         aGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACt                                                                                                                        <  8:41423/135‑1 (MQ=255)
         aGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGGCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACt                                                                                                                        <  5:202153/135‑1 (MQ=255)
              ggCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCAcc                                                                                                                   >  4:400/1‑135 (MQ=255)
                  aGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACCCCGTCGGCCCCGACCTGTTCCCACCCCTGATCCCGCAGCGGCACCGCGTGGCGCCGCCAATGCCGCGGGCCATCGCAGAAAACTTCACCCCCg                                                                                                               >  4:135586/1‑135 (MQ=255)
                  aGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCAc                                                                                                                >  4:55503/1‑134 (MQ=255)
                  aGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCACGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCAc                                                                                                                >  8:190106/1‑134 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTGAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  8:252436/135‑1 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGTCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  3:55503/135‑1 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  2:56664/135‑1 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  7:179053/135‑1 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              >  3:399122/1‑135 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  3:348326/135‑1 (MQ=255)
                   gAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGt                                                                                                              <  3:343299/135‑1 (MQ=255)
                         gCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGc                                                                                                         <  2:259719/134‑1 (MQ=255)
                                    cgctcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTgc                                                                                             <  5:1306/135‑1 (MQ=255)
                                    cgctcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTgc                                                                                             <  3:146921/135‑1 (MQ=255)
                                     gctcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTg                                                                                              <  5:154595/133‑1 (MQ=255)
                                       tcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCa                                                                                          >  5:87608/1‑135 (MQ=255)
                                       tcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCa                                                                                          <  7:44180/135‑1 (MQ=255)
                                          aGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCCCCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAACGCCGCTGGACATCGAAGAAAACTTCACCCACGTACTGCGTGACAAGCTGCGCAACg                                                                                       >  6:286790/1‑135 (MQ=255)
                                            gTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGc                                                                                     <  6:89679/135‑1 (MQ=255)
                                              cGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCCCGTGCCGCCCCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACCTACAGCGTGCAAAGCTGCGCAACGGCGa                                                                                   >  5:453020/1‑135 (MQ=255)
                                                   ccATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTgg                                                                              >  2:207808/1‑135 (MQ=255)
                                                   ccATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTgg                                                                              >  5:128367/1‑135 (MQ=255)
                                                      tCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAcg                                                                           <  7:118414/135‑1 (MQ=255)
                                                         acacCGTCGGCCCCTCCCTGTTCCCCCACCTGATCCCGCAGCTGCAACGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCCCCCACGTACAGCCTGAAAAGCTGCGCCACGGCGAACGGGACGCgg                                                                        >  5:237668/1‑135 (MQ=255)
                                                          cacCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCgg                                                                        >  1:142311/1‑134 (MQ=255)
                                                           acCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATGGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAcgc                                                                          <  8:404856/131‑1 (MQ=255)
                                                           acCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGt                                                                       <  6:87608/134‑1 (MQ=255)
                                                              gTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGatc                                                                   >  3:279753/1‑135 (MQ=255)
                                                              gTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGatc                                                                   >  1:287844/1‑135 (MQ=255)
                                                                  gCCCCTTCCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATGGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  3:86375/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          <  3:24175/76‑1 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                                                                          >  4:24175/1‑76 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  3:401324/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  3:167435/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  8:158017/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  5:58644/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCg                                                               >  5:364256/1‑135 (MQ=255)
                                                                  gCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGGGATCATCg                                                               >  1:238473/1‑135 (MQ=255)
                                                                   ccccTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGc                                                              >  1:334263/1‑135 (MQ=255)
                                                                    cccTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGcc                                                             <  2:334263/135‑1 (MQ=255)
                                                                          cTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGcc                                                        <  2:270773/134‑1 (MQ=255)
                                                                          cTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCg                                                       >  6:442218/1‑135 (MQ=255)
                                                                              tCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCa                                                   <  4:433328/135‑1 (MQ=255)
                                                                              tCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCa                                                   <  4:43428/135‑1 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCGACGTACTGCGTGAAAAGCTGCGCAAGGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  <  2:368053/134‑1 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGATCTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  7:265937/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  7:219730/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  7:262841/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  3:242925/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  2:82652/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  5:74639/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTCGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  5:21219/1‑134 (MQ=255)
                                                                                ccGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCAGTACATGGAAGAAAACATCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCCAACTGGACGCGGTGATCATCGCCCTGCCGTTCaa                                                  >  1:439529/1‑134 (MQ=255)
                                                                                 cGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAAc                                                 >  5:463702/1‑134 (MQ=255)
                                                                                    aCCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAg                                             >  3:448990/1‑135 (MQ=255)
                                                                                    aCCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAg                                             <  7:158017/135‑1 (MQ=255)
                                                                                           cccGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGt                                      <  7:241313/135‑1 (MQ=255)
                                                                                               cAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTg                                  <  7:337397/135‑1 (MQ=255)
                                                                                                aGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACAAAGCCGACGTGCTGa                                 <  5:217377/135‑1 (MQ=255)
                                                                                                  cTGCACCGCGTGGCGCCGCAATTGCCGCTGTACATCGAAGAAACCTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGGGAACTGGACGCGGTGATCATCGCCGTGCCGTTCAACGAAGCCGACGTGCTGACg                               <  6:453020/135‑1 (MQ=255)
                                                                                                  cTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAATAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACg                               <  4:393697/135‑1 (MQ=255)
                                                                                                  cTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACg                               <  5:422720/135‑1 (MQ=255)
                                                                                                  cTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACg                               <  4:358428/135‑1 (MQ=255)
                                                                                                   tGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGc                              <  6:72098/135‑1 (MQ=255)
                                                                                                       ccGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTAcc                          <  7:190106/135‑1 (MQ=255)
                                                                                                       ccGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTAcc                          <  1:281373/135‑1 (MQ=255)
                                                                                                          cgTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCt                       >  8:411303/1‑135 (MQ=255)
                                                                                                            tGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCtat                     <  4:401324/135‑1 (MQ=255)
                                                                                                             ggCGCCGCAAATGCCGCTGTACATGGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGCAGCCGACGTGCTGACGCTACCGCtata                    >  1:352344/1‑135 (MQ=255)
                                                                                                             ggCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCtata                    >  8:202080/1‑135 (MQ=255)
                                                                                                             ggCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCtata                    >  6:57339/1‑135 (MQ=255)
                                                                                                                 ccgcAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGAt                <  6:74639/135‑1 (MQ=255)
                                                                                                                 ccgcAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGAt                <  6:128367/135‑1 (MQ=255)
                                                                                                                 ccgcAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGAt                <  4:242925/135‑1 (MQ=255)
                                                                                                                 ccgcAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGAt                <  4:150542/135‑1 (MQ=255)
                                                                                                                               gTACATGGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACg                               <  8:77425/106‑1 (MQ=255)
                                                                                                                               gTACATGGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACg                               >  7:77425/1‑106 (MQ=255)
                                                                                                                                 aCATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGATGAACCGTTCTgcgc  <  8:133905/133‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
AGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTGCCGTTCAACGAAGCCGACGTGCTGACGCTACCGCTATACGATGAACCGTTCTGCGC  >  ACB122/6048768‑6049029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: