Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,072,767 A→G 6.1% I19V (ATC→GTC)  yeaD_2 → Putative glucose‑6‑phosphate 1‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,072,7670AG 6.1% 204.1 / 3.7 65I19V (ATC→GTC) yeaD_2Putative glucose‑6‑phosphate 1‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (36/25);  new base G (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CGCTGAGCCACGCTGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGC  >  ACB122/6072636‑6072900
                                                                                                                                   |                                                                                                                                     
cgCTGAGCCCCGCTGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGCGCAACATGGCGAACTCAACTCCTGGCGTATca                                                                                                                                    >  5:59036/1‑135 (MQ=255)
      gCCACGCTGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATcagcag                                                                                                                                <  3:460235/133‑1 (MQ=255)
             tGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGc                                                                                                                       >  6:132769/1‑135 (MQ=255)
                cGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGa                                                                                                                    <  8:393541/135‑1 (MQ=255)
                  gTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACTCAATGGCTACCTTTCAGGTCGTAACAGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAAc                                                                                                                  >  5:18731/1‑135 (MQ=255)
                   tAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTCACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCgctct                                                                                                                 >  6:17884/1‑131 (MQ=255)
                   tAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGCACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACt                                                                                                                 >  8:206268/1‑135 (MQ=255)
                           ttGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATc                                                                                                          <  1:193490/134‑1 (MQ=255)
                           ttGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCGACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATc                                                                                                          <  3:167528/134‑1 (MQ=255)
                            tGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTGTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCTGCAGTGACCGTGCCGAACTCCTGATCGc                                                                                                        >  1:387491/1‑135 (MQ=255)
                            tGCATCTGCCCGGGTCCAAGGCCCACTGGCAAGCAATGAGAAGCACGCAATGGCTACCTTTAAGGTAGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGc                                                                                                        >  7:137771/1‑135 (MQ=255)
                             gCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGCACTCCTGATCGc                                                                                                        >  8:60881/1‑134 (MQ=255)
                             gCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGcc                                                                                                       >  8:53329/1‑135 (MQ=255)
                               aTCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                     <  7:367351/135‑1 (MQ=255)
                               aTCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                     <  8:202791/135‑1 (MQ=255)
                               aTCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                     <  1:174489/135‑1 (MQ=255)
                                    cccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGCCCGTGCCGCACTCCTGATCGCCCAGCAgg                                                                                                >  4:357863/1‑135 (MQ=255)
                                     ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCcagc                                                                                                   <  4:146311/131‑1 (MQ=255)
                                     ccGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAggg                                                                                               >  1:4650/1‑135 (MQ=255)
                                       tggTCCAAGGCCCACTGGCAATCAATGAGAAGCACGAAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGAGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgcg                                                                                             <  6:262194/134‑1 (MQ=255)
                                       gggTCCAAGGCCCACTGGCAATCAATGAGAAGCACGCAATGGCTACCATTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgcg                                                                                             >  7:342356/1‑135 (MQ=255)
                                       gggTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGACCTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgcg                                                                                             >  5:228900/1‑135 (MQ=255)
                                       gggTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgcg                                                                                             <  3:161018/135‑1 (MQ=255)
                                           ccAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGcaca                                                                                         >  7:125729/1‑135 (MQ=255)
                                           ccAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGcaca                                                                                         >  7:7728/1‑135 (MQ=255)
                                           ccAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGcaca                                                                                         >  1:276072/1‑135 (MQ=255)
                                            cAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTGTCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGcaccg                                                                                        >  6:184548/1‑133 (MQ=255)
                                            cAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTGTCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAg                                                                                        >  6:176830/1‑135 (MQ=255)
                                              aGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGt                                                                                      <  5:219079/135‑1 (MQ=255)
                                                     cTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGc                                                                               <  1:277948/135‑1 (MQ=255)
                                                      tGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCt                                                                              >  1:187844/1‑135 (MQ=255)
                                                       ggCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTa                                                                             >  5:337771/1‑135 (MQ=255)
                                                         caatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTAc                                                                            <  8:385468/134‑1 (MQ=255)
                                                          aatcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACca                                                                          <  1:27992/135‑1 (MQ=255)
                                                                     agaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGc                                                                >  6:407324/1‑134 (MQ=255)
                                                                          gCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccg                                                          >  8:376046/1‑135 (MQ=255)
                                                                          gCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccg                                                          >  1:16245/1‑135 (MQ=255)
                                                                            aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccgcc                                                        >  2:120566/1‑135 (MQ=255)
                                                                             aTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccgccg                                                       <  3:163180/135‑1 (MQ=255)
                                                                                 cTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgc                                                   >  4:187940/1‑135 (MQ=255)
                                                                                 cTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgc                                                   >  2:169128/1‑135 (MQ=255)
                                                                                      tttCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTGTCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                              <  5:184548/135‑1 (MQ=255)
                                                                                      tttCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTGTCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                              <  5:176830/135‑1 (MQ=255)
                                                                                      tttCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                              >  1:444923/1‑135 (MQ=255)
                                                                                      tttAAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGAGAACCGCCGCTGCTGTgg                                              >  3:145279/1‑135 (MQ=255)
                                                                                         cAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGc                                             >  1:182313/1‑133 (MQ=255)
                                                                                         cAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGAGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGc                                             <  8:342356/133‑1 (MQ=255)
                                                                                             tCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCg                                       <  3:427168/135‑1 (MQ=255)
                                                                                                 aaCCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCa                                   <  8:125729/135‑1 (MQ=255)
                                                                                                  aCCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCCGCAGGTAGGCGCACCGCCGCTGCTGTGGCTGCGCGACCAg                                  >  5:71551/1‑135 (MQ=255)
                                                                                                  aCCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCa                                   <  8:7728/134‑1 (MQ=255)
                                                                                                  aCCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAg                                  >  7:72129/1‑135 (MQ=255)
                                                                                                  aCCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAg                                  >  4:61840/1‑135 (MQ=255)
                                                                                                      aGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCa                              <  6:18731/135‑1 (MQ=255)
                                                                                                         aaCATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCt                           >  7:406982/1‑135 (MQ=255)
                                                                                                         aaCATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCt                           >  5:152421/1‑135 (MQ=255)
                                                                                                             tGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgc                                                   <  6:261099/107‑1 (MQ=255)
                                                                                                             tGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctgc                                                   >  5:261099/1‑107 (MQ=255)
                                                                                                              ggCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGc                      >  2:306624/1‑135 (MQ=255)
                                                                                                                cGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCa                    <  7:35123/135‑1 (MQ=255)
                                                                                                                cGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCa                    <  6:228900/135‑1 (MQ=255)
                                                                                                                               gCGTATCCGCAGTTCCCGGGCGCAACTCGTGAGCCCCCAGCTGGGCGCACAGGGCCTCAGCTCCACCCAGGTAGGCGAACCGCCGCTGCTGGGGGTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGGCGGAAcg     <  6:121564/135‑1 (MQ=255)
                                                                                                                               gCGTATCAGCAGTGACCGTCCCCAACTCCTGAGCGCCCAGCAGGGCGCACAGGGCCTCCGCTCCCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGGCGGAAcg     <  2:276072/135‑1 (MQ=255)
                                                                                                                                 gTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAcgcg   >  8:424645/1‑135 (MQ=255)
                                                                                                                                  tATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACgcgc  >  2:98454/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CGCTGAGCCACGCTGGCGGTAAACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGAGCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGC  >  ACB122/6072636‑6072900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑