Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 478,896 T→G 28.2% D30A (GAC→GCC)  apaH_1 ← Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122478,8960TG28.2% 79.1 / 5.1 66D30A (GAC→GCC) apaH_1Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical
Reads supporting (aligned to +/- strand):  ref base T (37/7);  new base G (5/14);  total (42/24)
Fisher's exact test for biased strand distribution p-value = 1.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAGGGGGCAATC  >  ACB122/478762‑479026
                                                                                                                                      |                                                                                                                                  
gCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCCCAGGCGGt                                                                                                                                    >  5:427627/1‑135 (MQ=255)
gCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCCCAGGCGGt                                                                                                                                    >  6:116987/1‑135 (MQ=255)
 ccAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCCCAGGCGGTc                                                                                                                                   >  6:406311/1‑135 (MQ=255)
  cAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCCCCGGCGGTCg                                                                                                                                  >  6:414350/1‑135 (MQ=255)
  cAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCg                                                                                                                                  >  7:326302/1‑135 (MQ=255)
  cAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCACCAGCCACCCGCGGTCg                                                                                                                                  >  5:112015/1‑135 (MQ=255)
    ggcgccCCGCCGGGGCAGGACAGGGGGCCCCACCACGCAGACCAGTCAATGACGCACCGAGAACCGGACGCGCAGGGTTACCAGTCACGCGGGCCCGCGGTGGACCAGGCCGCCGACCAGCCACAGGCGGCCGAc                                                                                                                                <  4:218626/135‑1 (MQ=255)
       ggcCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCACAGGCGGTCGAcggc                                                                                                                             >  6:193745/1‑135 (MQ=255)
         ccAGCAGGTGCGGGTCTGGGTGGCCCCGCCCGCAGACCAGTGAAGGACGGACCGAGTACAGGAAGCGCCGGGTTTCCCGGGACGCGGGGCCGCGGTTCACCAGGGCGCCGACCAGCCACAGGCGGGCGAcggcgg                                                                                                                           <  2:420/135‑1 (MQ=255)
              aggtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGGCCCCCCCCCGGCGGGCGACGGGGGGGTTg                                                                                                                      >  2:430838/1‑135 (MQ=255)
                  gcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCCCCGGCGGGCGGCGGGGGGGGTggcac                                                                                                                  >  6:28839/1‑131 (MQ=255)
                             ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTGTCCAGTGACTCGGGGCCGCGGTTGACCAGGGCGCCGAGCACCCCCAGCCGGTCGCGTGCGGGGTTGAAACTGACCCGATcc                                                                                                       >  2:408113/1‑134 (MQ=255)
                             tggCCCACCCCGCAGCCCAGTGATGGCCGGAGCGATAACCGGCCGCGCCGGGTTACCAGTGCCTGGGGGCGGCGGTGGACGAGGGCGCCGACCAGCCCCAGGGGGGCGACGGCGGGGTTGAAACTGACGCGATCg                                                                                                       <  4:324792/133‑1 (MQ=255)
                              tGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGGGGACGGCGGGGTTGGAACTGTcgcgggcgc                                                                                                      >  6:455917/1‑130 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGACCCGCCACAGGCGGTCGCCCGCGGGGTTGACACTGACGCGATCGAGCAgg                                                                                                 >  3:240093/1‑135 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTCGACGGCGGGGTTGGAACTGCCGCGCTCGAGCAgg                                                                                                 >  7:285746/1‑135 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAg                                                                                                  >  8:78552/1‑134 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCAAACGCCGTAGAAGGCGGGGTTGAAAATGACTAGAACGATCAg                                                                                                  >  5:186984/1‑134 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAg                                                                                                  >  4:416611/1‑134 (MQ=255)
                                   aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAg                                                                                                  >  7:127888/1‑134 (MQ=255)
                                        gCAGACCAGTGAATGACGGATCGATTACAGTACGCGCTGGTTTTCCCTGGACGCGGGGCCGGGGTTGACCAGGGCGCCGACCAGCCACTGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                                            <  8:313676/135‑1 (MQ=255)
                                        gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCACTGACTCGGCGCCGCGGTAGACCAGGTCGCCGACCAGCCACACCCCGTCGACGGCCGGGTAGAAACTGACGCGCTCGAGCAGGCAtt                                                                                             >  2:31439/1‑134 (MQ=255)
                                        gCAGACCAGTGAATGACGGATCGAGAACAGGAAGCGCAGGGTTTCCAGGGACTCGGGGCCGCGGTTGACCAGGGCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                                            <  3:111466/135‑1 (MQ=255)
                                        gCAGACCAGTGAATGACGGAGCTAGAACAGGACGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                                            <  3:231999/135‑1 (MQ=255)
                                             ccAGTGACTGACGGATCGAGTACAGGACCCGCAGGGTTTCCAGTGCCTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCg                                                                                       <  4:351151/135‑1 (MQ=255)
                                             ccAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCg                                                                                       >  2:107724/1‑135 (MQ=255)
                                                  gatggACGGCGCGTGTACCGGACGCGCTGGGTTTCCAGTGCCTCGGGGCGGGGGTTGACCAGGGCGCCGACCAGCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgc                                                                                  <  2:442121/131‑1 (MQ=255)
                                                  gAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCt                                                                                    >  4:14743/1‑133 (MQ=255)
                                                    aTGACGGATCGAGTACAGGAATACCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACCATCGGTAGACGCCGGGGGTGAAAATTACCCGATAGTGCCGGAATATGAGCCGCTgcag                                                                                >  2:290437/1‑135 (MQ=255)
                                                    aTGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACCGCGGGGTTGAAACTGACGCGATCGAGCACGCAATTTCGCGGCTgcag                                                                                >  8:71870/1‑135 (MQ=255)
                                                     tGACGTATCGAGAACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgcagg                                                                               <  6:128209/135‑1 (MQ=255)
                                                         ggATCGAGTACAGGATGAGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGACGAACAGCCACAGGCCGTCCACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTgcaggcacc                                                                           >  4:346118/1‑133 (MQ=255)
                                                         ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTAGCCGGCGGGGTTGAAACTGACGCGATAGAGCAGGCAATTTAGCGGCAGCAGGCAGc                                                                           >  6:80701/1‑135 (MQ=255)
                                                              gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACCGGCGGTCGCCGGCGGGGGTGAAACTGACGCGCTCGCGCAGGCATTTGAGCGGCTGCAGGCAGCCCt                                                                        >  1:415880/1‑133 (MQ=255)
                                                              gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCCCCCCCGGCGGGCGCCGGCGGGGTTTAAAATGCCGCGCTCGAGCAGGGAATTGAGCGGCGGCAGGCAGcccg                                                                        >  5:249035/1‑132 (MQ=255)
                                                              gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCt                                                                        >  3:138319/1‑133 (MQ=255)
                                                              gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCt                                                                        >  4:444554/1‑133 (MQ=255)
                                                              gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCACCCGCCCCACGCGGGCGACCGCGGGGTTGAAACGGACGCGATCGAGCACGCATTTGCGCGGCTGCAGGCCGCCCTGc                                                                      >  6:138850/1‑135 (MQ=255)
                                                                gTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGCCGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAg                                                                    >  4:187985/1‑135 (MQ=255)
                                                                   ccgcacgcgcAGGGTTCCCCGTGACGCGGGGCCGCGGTTGACCCGGGCGCCCACCAGCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTc                                                                 <  4:403022/129‑1 (MQ=255)
                                                                    aGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCa                                                                >  7:332555/1‑135 (MQ=255)
                                                                         gcgcAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGAc                                                           >  1:117331/1‑135 (MQ=255)
                                                                           gctgggTTGCCAGTGACGCGGGGCCGCGGTGGCCCAGGGCGCCGACCAGCCCCCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACgg                                                         <  6:7228/132‑1 (MQ=255)
                                                                           gcAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCCGGCATTTGAGCCGCTGCAGGCAGCCCTGCAGGTCCCCGACgg                                                         >  4:288814/1‑135 (MQ=255)
                                                                           gcAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACgg                                                         <  4:320340/135‑1 (MQ=255)
                                                                            cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCCCAGGCGGTCGACGGCGGGGTTGAAACTGACGCGCTCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCggcggc                                                        >  3:274993/1‑135 (MQ=255)
                                                                            cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCACCCACAGGCGGTCGACGGGGGGGTTGAAACAGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCAGGCAGGTCACCGCCGGc                                                        >  6:131645/1‑135 (MQ=255)
                                                                            cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGCAACTGACGCGATCGAGCAGGCCTTTGAGCGGCTGCAGGCCGCCCTGCAGGTCACCGACGGc                                                        >  8:425122/1‑135 (MQ=255)
                                                                                tttcccAGGGCCTCGGGGCCGCGGTGCACCCGGTCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAg                                                    <  5:175920/131‑1 (MQ=255)
                                                                                     cAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGc                                               <  8:326302/135‑1 (MQ=255)
                                                                                      tgTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACCGGGGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGc                                               <  6:205477/133‑1 (MQ=255)
                                                                                            tCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCCGGCAGCCCTGCAGGTCACCGCCGGCGGAGGTGGCCATCAGt                                        >  2:53186/1‑135 (MQ=255)
                                                                                            tCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                                        >  7:129835/1‑135 (MQ=255)
                                                                                            tCGGGGACGCGGTTGACCAGGTCGCCGACCAGCCACACGCGGTCCACCGCGGGGTTGAAACTTACGCGATAGACCACGCAATTGAGAGGCTGAAGCCATCCCTGCAAGTAACCCCCCGCGTATGTGGCCATCat                                         >  4:41552/1‑133 (MQ=255)
                                                                                            gcGGGGCCGCGGTGGCCCCGGGCGCCGACCCCCCCCCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                                        <  5:414350/134‑1 (MQ=255)
                                                                                             cGGGGCCGGGGTTGACCAGGGCGCCGACCAGCCACCGGCGGGCGACGGCGGGGGTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTg                                       <  8:269045/135‑1 (MQ=255)
                                                                                              ggggCCGCGGTGCCCCCGGCCGCCCACCCCCCCCCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAGTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGc                                      <  6:50803/135‑1 (MQ=255)
                                                                                                  ccGCGGTTGACCAGGTCGCCGACCAGCCCCAGGCGGTCGCCGGCGGGGGTGAAACTGACGCGATCGCGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGGAGGTGGCCCTCAGTGCAgcg                                  >  7:399960/1‑135 (MQ=255)
                                                                                                  ccGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGCAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACCGCGTAGGTGGCCATCCGTGCAgcg                                  >  4:119970/1‑135 (MQ=255)
                                                                                                      gtgtGACCCGGGCGCCCACCAGCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTcc                              <  5:357037/132‑1 (MQ=255)
                                                                                                        ttGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGAAGGCGTAGGTGGCCATCAGTGCAGCGCTCCgg                            >  2:279126/1‑135 (MQ=255)
                                                                                                            ccAGGGCGCCGACCAGCCCCAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCAc                        <  8:19511/135‑1 (MQ=255)
                                                                                                                gcggCCGACCAGCCACCGGCGGCCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGcc                    <  3:421852/132‑1 (MQ=255)
                                                                                                                gTCGCCTACCAGCCCCCGGCGGCGGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGcc                    <  5:210617/135‑1 (MQ=255)
                                                                                                                   gCCGACCACCCACCTGCGGTCGTCGGAGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCACGCAGCCCTGCAAGTCACCCAAGGCGTAGGTGGCCATCAGTGCACCCCACCGGGCACTGCCAGc                 >  7:308340/1‑135 (MQ=255)
                                                                                                                     cGACCAGCCACCGGGGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCg               <  6:427627/135‑1 (MQ=255)
                                                                                                                             caaaagCGGTAGACCGCGGGGGTGCAAAGGCCCCGCTCTAGCAGGCATTTGAGCCGCTTCACGCAGACATACACGTCCCCCCCGGCGAAGGTGGACCAAAGTGCAGCGCTCCGGGCCCTTCAACCCGCAAggggg       >  8:21088/6‑135 (MQ=255)
                                                                                                                                  gCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAGGGGGCAAtc  <  4:112985/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCGGAAGGGGGCAATC  >  ACB122/478762‑479026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: