| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 539,482 | A→T | 14.6% | V87V (GTA→GTT) | rpsL → | 30S ribosomal protein S12 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 539,482 | 0 | A | T | 14.6% | 135.6 / 5.3 | 59 | V87V (GTA→GTT) | rpsL | 30S ribosomal protein S12 |
| Reads supporting (aligned to +/- strand): ref base A (19/31); new base T (7/2); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGTCCGAAGTAATCGGT > ACB122/539351‑539598 | aaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTaaaa > 5:88544/1‑135 (MQ=255)aaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTa > 4:43674/1‑132 (MQ=255) cTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTGTGACCAAGGGTTTCGATGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGTCCGTGTAAAAGAC‑t < 3:360458/135‑1 (MQ=255) gacaggCGTAAAGTTTGCCGTGTGCGTCTGACCCTGGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGGGGCGGCCGTGTTAAAGAC‑TTGCCAGgt < 5:330103/130‑1 (MQ=255) gacaggCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 5:424679/130‑1 (MQ=255) gCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 8:167348/135‑1 (MQ=255) gCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 6:204232/135‑1 (MQ=255) gCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 5:6934/135‑1 (MQ=255) gCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 2:89182/135‑1 (MQ=255) gCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCATCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 8:292443/135‑1 (MQ=255) gCACTGCCTAAAGCATGCCGGGTGCGGCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGgt < 2:448074/135‑1 (MQ=255) tGCGTAAAGTATGCCGTGTGCGTCTGACCAACTGTTTCGAGGTTTCCTCGTACATCGCTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTc > 7:319725/1‑135 (MQ=255) cGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGt < 7:175128/135‑1 (MQ=255) aaaGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑ATGCCCGGGGTTCGTTAc > 1:325659/1‑135 (MQ=255) cGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGt < 4:369344/134‑1 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACCGCGTCGTCCTGAATCGTGGCGGCCGTGTTAAAGAC‑TTGCCAGGTGTTAGTTACCAAAccaggc > 6:424679/1‑131 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGCC‑TTGCCAGGTGTTCGTTTCCCCACCGTTc > 1:319134/1‑135 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTc < 3:439450/135‑1 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTc < 4:215035/135‑1 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGGAAAAGAC‑TTTCCAGGTGTTAGTTACCACACCGTTc > 8:175128/1‑135 (MQ=255) gtgtGCGTCTGACCAACGGTTTCGAGGGTTCATCGTACATCCGTGGTGAAGGCCACAACCTGCAAGAGCACACCGTCGTCCTGAATACTTGCGGCCGTGTTAAACACATTCCCAGG‑GTTCGGTAACAAAcccaac > 6:330103/1‑131 (MQ=255) gtgCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGTACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCTTTACCACACCGTTcgc < 4:220858/135‑1 (MQ=255) gTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTTAAAGAC‑TTGCCAGGTGTTAGTTTCCACACCCTTAGCGGct > 7:161667/1‑135 (MQ=255) gTCTGACCAACGGTTTCGAGGTTTCCTCATACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCACGTGTTCGTTACCACACCGTAcgccgc > 7:316183/1‑134 (MQ=255) gTCTGACCAACGGTTTCGAGGTTTACTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTAGTTGCGGCCGTGTAAAAAAC‑ATGCCAAGTGTTCGGTAACACACCGGTAGCGGcg > 2:119157/1‑134 (MQ=255) aCCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGGAAAAGAC‑TTTCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTg > 8:440730/1‑135 (MQ=255) ccAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGctctct < 8:32469/133‑1 (MQ=255) gTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACtt < 7:38172/135‑1 (MQ=255) ggTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑Gt < 4:332510/135‑1 (MQ=255) ggTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑Gt < 3:43674/135‑1 (MQ=255) ggTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑Gt < 7:320317/135‑1 (MQ=255) tCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGGTAAATAC‑TTTGCAAGTGTTCGTTTACACCCCCTTAGCCGCTGTCTGGTTACTTCGGGG‑GTTtaa > 6:60806/1‑135 (MQ=255) tCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTGGTTTCCACACCCTTGGCGGCGCTCTGGATACTTCGGGG‑GTTTAAGGc > 7:406621/1‑135 (MQ=255) tCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGc > 3:445663/1‑135 (MQ=255) gtggtgAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAg < 6:158007/134‑1 (MQ=255) gtggtgAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAg < 1:57096/134‑1 (MQ=255) tggtgAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGGAAAAGAC‑ATGGCAGGTGTTTGTTACCACCCCGGTGGCGGGTCGGTGGGTACCTGGGGC‑GGTAAAGGCCGGTACCCggg > 3:246526/1‑135 (MQ=255) ggtgAAGGTCACAACCTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCGTGTTAAAGAC‑CTCCCGGGTGTTCGTTACCACACCGTACGTGGTGCGCTTGACTGCTCCGGC‑GTTAAAGACCGTAAGCAggct < 1:446663/135‑3 (MQ=255) taaGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGt < 2:107933/134‑1 (MQ=255) gAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGt < 1:108503/135‑1 (MQ=255) gAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGt < 1:73481/135‑1 (MQ=255) gAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGt < 6:88544/135‑1 (MQ=255) gAAGGCCACAACCTGCAAGAGCACAGCGTAGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGGTAGTTACCACACCGTTCGCGTCTATATGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGTGTCGt < 8:28044/135‑1 (MQ=255) cagggCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGt < 7:324224/132‑1 (MQ=255) aaGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCGCTGGATTCTTCGGGC‑GTTAAAGGCCGTTACCAGGGTCggt > 7:114381/1‑133 (MQ=255) gCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGAGACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTc > 6:169141/1‑133 (MQ=255) gCCACAACCTGCAAGAGCACAGCGTCGTCCGGCTTCGTGGCGGCCGGGGTAAAGAC‑ATGCCACGGGGTCGTTACCCCACCGGTCGCGGGGGGCAGGATTCTTCGGGCGGTTAACGCCCGCACCCAgggggggc > 2:180888/1‑128 (MQ=255) ccACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGaa < 8:155757/135‑1 (MQ=255) ccACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTGCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGaa < 5:60806/135‑1 (MQ=255) cacaACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTTAAAGAC‑TTGCCAGGTGTTCGTTTCCACACCCTTCGCGGCGCTCGGGATACTTGGGGC‑GGTTAAGGCCGTAACCAGGGTCGtggggag > 5:149417/1‑129 (MQ=255) cacaACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGaa > 8:317397/1‑134 (MQ=255) acaACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGGAAAAGAC‑TTGCCAGGGGTTCGTTACCACACCCGTCGCGGCGCGCGGGCTACTTCGGGC‑GTTAAAGGCCGGACCCCCGGTCGtgggcag > 6:5128/1‑128 (MQ=255) acTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑GTGCCAGGTGTTCGTTACCACACCGGTCGCGGCTCTCGTGATACTTCGGGC‑TGTAAATGCCGTAACCAGGGTCGTTCGAAGTaag > 4:231784/2‑132 (MQ=255) agagCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCGCGGGATACGTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTGGAAGTG‑CGGTAcctg > 8:324224/1‑133 (MQ=255) gagCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTTAAAGAC‑ATTCCAGGGGGTCGTTTCCACACCCGTGGCCGCGCTGTGGTTTCATGGGGG‑GGTATATGGCGTAAACACGGGCGTTGGGAGTAGCGGTAcaca > 2:464079/1‑132 (MQ=255) gagCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACCCCGTTCGCGGCTCTCTGGATACCTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCCAg > 8:74624/1‑135 (MQ=255) aGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGGcc > 3:422916/1‑134 (MQ=255) tcgtcCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGTCCGAAGt < 1:119157/135‑1 (MQ=255) tcgtcCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTAACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGTCCGAAGt < 5:5128/135‑1 (MQ=255) tGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACATCGGGC‑GGTAAAGGCCGTAAACAGGGTCGTTGGAAGTA‑AGGTACCAAGCGTCCCAAGTAAACgg > 8:107682/1‑135 (MQ=255) ttCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGTCCGAAGTAATCGGt < 3:228868/133‑1 (MQ=255) | AAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGAC‑TTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGC‑GTTAAAGGCCGTAACCAGGGTCGTTCGAAGTA‑CGGTACCAAGCGTCCGAAGTAATCGGT > ACB122/539351‑539598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |