| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 594,746 | C→T | 6.0% | S347S (AGC→AGT) | ribD → | Riboflavin biosynthesis protein RibD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 594,746 | 0 | C | T | 6.0% | 249.7 / 3.8 | 84 | S347S (AGC→AGT) | ribD | Riboflavin biosynthesis protein RibD |
| Reads supporting (aligned to +/- strand): ref base C (39/39); new base T (3/2); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
GCCGGTGCCGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTGAGGAAGACCC > ACB122/594618‑594877 | gccggggccgggCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGGCGACGAATACCAGCTGTTCGTCGCTGGCAACTTCGTCGGCTCACAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGca < 4:354057/135‑1 (MQ=255) ccGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGCTGGACAAGATGAGCGAAGCCCCACCGc > 6:283554/1‑135 (MQ=255) ccGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGTGAAGCACCACGGc > 4:233165/1‑135 (MQ=255) ccGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGTGAAGCACCACGGc > 4:225403/1‑135 (MQ=255) ccGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCCCCACGGc > 3:455627/1‑135 (MQ=255) ggTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCGACTGGGCTGGCCGATGGCCCAGATGTGCGAAGCACCACGGCTGAAAATTAcc > 6:260635/1‑135 (MQ=255) cTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGaa < 1:145057/133‑1 (MQ=255) cTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGaa > 2:145057/1‑133 (MQ=255) cTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAAt > 3:309577/1‑135 (MQ=255) cTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCGACTGGACTGGCCGCTGGACAAGCTGAGCGAAGCACCACGGCTGAAAATTACCGAAAt > 1:119757/1‑135 (MQ=255) cTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCGACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAAt > 1:140097/1‑135 (MQ=255) gCACTGCATGGCCTGGTCGACGAATACCAGCTGTTGTTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTAGTGGCCTGTCCGTTGGTCAAGTTGAGCGAAGCACCACGGCTGAAAATTTCCGAAATgc < 8:369581/133‑1 (MQ=255) cACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCCCCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGCCTGGCCGATGGCCAAGATGAGAGAAGCCCCACGGCTGAAAATTAACGAACTgcgc > 1:412643/1‑134 (MQ=255) aCGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGC‑AAGCCCCACGGCTGAAAATTACCGAAATGCGCGCa > 8:35045/1‑135 (MQ=255) ccaGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCCCACTg > 4:428848/3‑135 (MQ=255) cAAGGCATGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACCGGACTGGCCGAGGGAGAAGATGAGCGAAGCACCCCGGCTGAAAATTACCGAAATGCGCGCAGTg > 8:337181/1‑135 (MQ=255) aGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGcg > 2:76541/1‑107 (MQ=255) aGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCt < 1:76541/108‑1 (MQ=255) aGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCt < 1:83717/108‑1 (MQ=255) aGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCt > 2:83717/1‑108 (MQ=255) cTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGCAGCCCCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGa > 7:302574/1‑134 (MQ=255) cTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGa > 7:302577/1‑134 (MQ=255) tCGACGAATCCCAGCGGTTCGTCGCTGGCCCCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGACCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACt < 5:377394/135‑1 (MQ=255) tCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGAc < 3:166816/134‑1 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCTCCTTCCTCGGCTCCCAGGCCCGGCCGCTCCTGGACAGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAAATCGCGCAGTGTGCGAGGACTg > 6:140831/1‑135 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGGCGATGGACAAGATGAGCGAACCCCCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGCTGCCTg > 2:288237/1‑135 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCCCGGCTGAAAATTACCGCAATGCGCGCAGTGGGCGATGACTg > 2:34805/1‑135 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTg > 6:192729/1‑135 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTg > 8:244678/1‑135 (MQ=255) cgacgaATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTg > 5:183265/1‑135 (MQ=255) ccaaTACCAGGTGTTCGTGGCTGGCACGTCCCTCGGCTCCCCGGCCCGGCCGCTACTGGACTGGCCGATGGACACGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCg < 5:283554/133‑1 (MQ=255) gaATACCAGCTGTTCGGCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCgg < 3:394773/135‑1 (MQ=255) aaTACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCggg < 1:375036/135‑1 (MQ=255) aTACCAGCTGTTCGTCGCTGGGACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCGGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCggg > 5:2779/1‑134 (MQ=255) aTACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCCGGCCCGGCCGCTACTGGACTGGGCGCGGGCCAAGATGAGCGCAGCCCCACGGCTGCAAATTACCGCAATTCGCGCCCTGGGCGCTGACTGGCGGGt > 1:302856/1‑135 (MQ=255) aTACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGt > 6:159676/1‑135 (MQ=255) cAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACg < 7:354851/135‑1 (MQ=255) cAGCTGTTCGTCGCTGGCACCTTCCTCGGCGCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACg < 1:424735/135‑1 (MQ=255) ttCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTTCTGGACTGGCCGATGGACAATATGAGCGAATCACCACGGCTGAAAATTACAGAAATGCGCGCAGTGGGCGATGACTTGCGGGTCAAGGCCATc > 8:1527/1‑135 (MQ=255) ttCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGCTGGACAAGATGAGCGAAGCACCACCGCTGAAAATTACCCTAATGCGCCCAGTGGGCGATGACTGGCGGGTCACGGCCATc > 2:359667/1‑135 (MQ=255) ttCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGAGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATc > 3:432632/1‑135 (MQ=255) cgtcgCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCCCCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATccc > 6:158980/1‑135 (MQ=255) gtcgCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGATCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATcccc > 8:41175/1‑135 (MQ=255) gtcgCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACg > 3:275954/1‑126 (MQ=255) gtcgCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACg < 4:275954/126‑1 (MQ=255) gtcgCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATcccc > 5:245648/1‑135 (MQ=255) gCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAg > 4:239489/1‑135 (MQ=255) cACCTTCCTCGGCTCCCAGGCGCGGCCTCTACTGGACTGTCGGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgc < 3:85211/135‑1 (MQ=255) cACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgc < 4:341020/135‑1 (MQ=255) cACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgc < 2:30313/135‑1 (MQ=255) cACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgc < 3:239489/135‑1 (MQ=255) ccTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGTGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCACCCCCCTGCCAgcgc < 3:225403/135‑1 (MQ=255) ccTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGTGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCACCCCCCTGCCAgcgc < 3:233165/135‑1 (MQ=255) ccTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgcgc < 4:262280/135‑1 (MQ=255) ccTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgcgc < 5:303904/135‑1 (MQ=255) ttCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGccc < 1:105605/135‑1 (MQ=255) tCGGCTCCCAGGCCCGGCCGCTACTGCACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCg < 1:445905/135‑1 (MQ=255) cTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCAGCCAGCCCCCCGCCtata > 1:437650/1‑135 (MQ=255) tCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATaa < 8:65387/135‑1 (MQ=255) tCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATaa < 4:448130/135‑1 (MQ=255) gcccAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATaa < 8:382922/134‑1 (MQ=255) gcccAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGAGGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATaa < 4:344261/134‑1 (MQ=255) ggCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCg < 1:145826/135‑1 (MQ=255) gCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGAGGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTg < 2:119757/135‑1 (MQ=255) actggCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCa < 4:194081/133‑1 (MQ=255) actggCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCa < 5:121934/133‑1 (MQ=255) ccGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGtt < 2:159969/135‑1 (MQ=255) gATGGACAAGATGAGTGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGtttt > 4:403966/1‑135 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCTCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgcgc < 8:276885/95‑1 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgcgc < 1:171373/95‑1 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAgcgc > 2:171373/1‑95 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCg < 7:389140/111‑1 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCg > 8:389140/1‑111 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGtttt > 3:449638/1‑135 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCCGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGtttt > 6:246920/1‑135 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCACCTGCCAGCGCCCCGCGGATAAATCCGCGCATTCCCCGCCCAAACCCtttt > 2:415973/1‑135 (MQ=255) gATGGACAAGATGAGCGAAGCACCACGGATGAAAATTACCGAAATGCGCGCAGTGGTCGATGACTGGAGGGTCAAGGCAATCCCCCTGCCAGCGACCCGCGTAATATGCCGGGCTAGCCCCGCGCAAACCCtttt > 6:170873/1‑135 (MQ=255) gATGGACAAGATGACCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGAGGGTCACGGCCATCCCCCTGCCAgcgc > 7:276885/1‑95 (MQ=255) ttgACAAGATGAGCGAAGCACCACGGCTGAAAATTACAGAAATGCGCGCAGTGTGCGATGACTGGCGGGTCACGGCCATCCGCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTg < 3:428848/133‑1 (MQ=255) tGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTg < 2:412643/135‑1 (MQ=255) gACAAGAGGCGCGAAGCACCACGGCTGTAACTTACCGATATGCGAGCAGTGGGCGATGACTGGCGGGTGACGGCCATGACCCTGCCAGCGCCCGGCGTTTATTGCCGTGCTTGCCCCGCGCAAACCGTTTTTGa < 8:238199/134‑1 (MQ=255) aCAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTGAgg < 2:140097/135‑1 (MQ=255) aCAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTGAgg < 8:284475/135‑1 (MQ=255) gAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTGAGGAAGAccc < 4:309577/135‑1 (MQ=255) | GCCGGTGCCGGCCTGGTCGGTGCCTTCGCACGGCAAGGCCTGGTCGACGAATACCAGCTGTTCGTCGCTGGCACCTTCCTCGGCTCCCAGGCCCGGCCGCTACTGGACTGGCCGATGGACAAGATGAGCGAAGCACCACGGCTGAAAATTACCGAAATGCGCGCAGTGGGCGATGACTGGCGGGTCACGGCCATCCCCCTGCCAGCGCCCGGCGTATAATGCCGGGCTTGCCCCGCGCAAACCGTTTTTGAGGAAGACCC > ACB122/594618‑594877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |