Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	891,894	G→A	5.9%	A64T (GCC→ACC)	CJ019_00856 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	891,894	0	G	A	5.9%	205.0 / 3.4	68	A64T (GCC→ACC)	CJ019_00856	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/36);  new base A (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AATTACAGCAGCACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGATTGACC  >  ACB122/891761‑892026                                                                                                                                      |                                                                                                                                     aaTTACAGACGCACTGCACATTCCGGTTCTCGTCGCCGGAGGTATAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGc                                                                                                                                     <  5:260306/135‑1 (MQ=255)    tACAGCACCACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTAATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGa                                                                                                                                  <  2:114155/135‑1 (MQ=255)      cagcagCACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAACGCGGCCGaaa                                                                                                                                <  4:142722/135‑1 (MQ=255)      cagcacCACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGCGACCGCCAGTTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGaaa                                                                                                                                <  7:330853/135‑1 (MQ=255)       agcagcACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGaaa                                                                                                                                <  8:44279/134‑1 (MQ=255)       agcagcACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGaaa                                                                                                                                <  3:83597/134‑1 (MQ=255)            caCTGCACAATCCGGATATCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGTCCCGCCAGTTAAACTCAGTGATAGGCGCACAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTAt                                                                                                                          >  5:185619/1‑135 (MQ=255)              cTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATcc                                                                                                                        <  1:117795/135‑1 (MQ=255)              cTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATc                                                                                                                         >  4:135176/1‑134 (MQ=255)                    aaTCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGCCTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCACACGTAAAGCAAGAGGCCGAAAGGGTAGCCCTGGat                                                                                                                  >  3:34828/1‑134 (MQ=255)                    aaTCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGcc                                                                                                                                    <  4:31839/117‑1 (MQ=255)                    aaTCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGcc                                                                                                                                    >  3:31839/1‑117 (MQ=255)                    aaTCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAg                                                                                                                  >  3:334212/1‑135 (MQ=255)                    aaTCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTGAAAGAAAGCGGCCGAAAGGGTATCCCTGGAg                                                                                                                  >  8:230699/1‑135 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTcc                                                                                                               >  1:196947/1‑133 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTcc                                                                                                               >  4:446138/1‑133 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTcc                                                                                                               >  6:189309/1‑133 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGCTATCCCTGGAGTcc                                                                                                               >  3:383094/1‑133 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATATAGGACTTTGGGGCCCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTcc                                                                                                               >  4:92161/1‑133 (MQ=255)                         ggATCTCGTCGCCGGAGGTAGAGACGTCATAACGGACTATGGGGACCTCCAGGTAAACTCAGTGATAGGCGCGCAATGGAAAACAAGGATACCCCACTTTAAGAAAGCGTCCGAAAGGCTATGCCTGGACTCCag                                                                                                             >  1:80023/1‑135 (MQ=255)                              tcgtcgCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGc                                                                                                         >  6:164653/1‑134 (MQ=255)                                 gcgCCGGAGGTAGAGACGCCATCTCGTACTTTGGGCACCGCAAGGTAAACTCAGGGATCGGCGCCCAATGCAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCagag                                                                                                           <  5:133721/128‑1 (MQ=255)                                   gCCGGAGGTAGAGACGTCATATCGGACTTTGGGGCCCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATc                                                                                                   >  5:14565/1‑135 (MQ=255)                                   gCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATATGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCATGGAGTCCAGAGGCTCAATc                                                                                                   >  1:423976/1‑135 (MQ=255)                                   gCCGGAGGTAGAGACGTCAAATCGGACTTTGGGGACCGCCAGGTAAACAAAGTGATAGGCTCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCGCaaaa                                                                                                   >  5:425682/1‑133 (MQ=255)                                      ggAGGTAGAGACGTCAAATCGGACTTTGGGGACCTCCAGGTAAACTCAGTCATAGGCGCCCAATCGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGc                                                                                                <  1:9506/135‑1 (MQ=255)                                           tAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATCCCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGa                                                                                            <  2:196947/134‑1 (MQ=255)                                              agaCGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATg                                                                                         <  4:270534/134‑1 (MQ=255)                                                     atatCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAAtt                                                                                  >  1:286271/1‑134 (MQ=255)                                                     atatCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTa                                                                                 <  6:9891/135‑1 (MQ=255)                                                      tctCGGAGTTTGGGTCCCCCGAGTTAAACTAAGTTAATCGCGCCCATTTAAACACCAGTATACCAAATTAAAATTAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTa                                                                                 <  5:278201/132‑1 (MQ=255)                                                      tatCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTa                                                                                 <  6:156158/134‑1 (MQ=255)                                                           gACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATaa                                                                            <  3:122064/134‑1 (MQ=255)                                                            aCTTTGGGGACCGCCTGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  2:286271/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGTTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  1:142890/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGGTAAACTCATTTATAGGCGCCCAATGGAAAACCAGTATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  2:423976/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  5:165986/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  6:157917/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  1:180251/135‑1 (MQ=255)                                                            aCTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  2:258294/135‑1 (MQ=255)                                                            aCTTTGGGGAACGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGTGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGt                                                                          <  4:142258/135‑1 (MQ=255)                                                                   ggACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAgg                                                                   >  2:49874/1‑135 (MQ=255)                                                                    gACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGt                                                                  <  1:49874/135‑1 (MQ=255)                                                                    gACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGt                                                                  <  3:135176/135‑1 (MQ=255)                                                                          cAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCAGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAg                                                            >  6:49090/1‑135 (MQ=255)                                                                             gTAAACTATGTGATAGGCGCCCAATGTAAAACCAGCATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGtt                                                         <  1:49908/135‑1 (MQ=255)                                                                                aaCTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGa                                                       <  1:115538/134‑1 (MQ=255)                                                                                      gTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCGATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATgg                                                  <  3:446138/133‑1 (MQ=255)                                                                                          tAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATaaa                                             >  3:428432/1‑134 (MQ=255)                                                                                          tAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGCAGTCCAGAGGCTCAATCCGCATGAATGACAAATAACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATaaa                                             >  1:108593/1‑134 (MQ=255)                                                                                               gcCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGaa                                                                                           >  4:160539/1‑83 (MQ=255)                                                                                               gcCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGaa                                                                                           <  3:160539/83‑1 (MQ=255)                                                                                                          aaCCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTttgat                            >  2:11178/1‑135 (MQ=255)                                                                                                             cAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTTCCAGTttgat                            >  2:247992/1‑132 (MQ=255)                                                                                                                  tACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTa                                          <  4:92272/113‑1 (MQ=255)                                                                                                                  tACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTa                                          >  3:92272/1‑113 (MQ=255)                                                                                                                  tACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATCTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAAtt                    >  6:87809/1‑135 (MQ=255)                                                                                                                          ttAAATAAAGAGGCCGAAAGGGTATCCATCGAGTCCACAGGCTAAATCAGCAAGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGATCAAATGGATAAAGTACGTGCCACTGTGATTGAGAATTTCGGTccg            >  5:39151/1‑135 (MQ=255)                                                                                                                          ttAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTccg            >  1:101029/1‑135 (MQ=255)                                                                                                                          ttAAAGAAAGCGACCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGtt                                                         >  6:144729/1‑90 (MQ=255)                                                                                                                          ttAAAGAAAGCGACCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGtt                                                         >  6:138136/1‑90 (MQ=255)                                                                                                                          ttAAAGAAAGCGACCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGtt                                                         <  5:144729/90‑1 (MQ=255)                                                                                                                          ttAAAGAAAGCGACCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGtt                                                         <  5:138136/90‑1 (MQ=255)                                                                                                                               gaaagCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATACGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGAt       <  6:14565/135‑1 (MQ=255)                                                                                                                               gaaagCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGAt       <  7:86795/135‑1 (MQ=255)                                                                                                                                aacgCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGAtt      <  7:364696/132‑1 (MQ=255)                                                                                                                                  cgCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGATTTTCGGTCCGCCGATTGa    <  5:181694/134‑1 (MQ=255)                                                                                                                                    cGGCCGAAAGGGGATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGATTGAcc  <  1:462045/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     AATTACAGCAGCACTGCACAATCCGGATCTCGTCGCCGGAGGTAGAGACGTCATATCGGACTTTGGGGACCGCCAGGTAAACTCAGTGATAGGCGCCCAATGGAAAACCAGGATACCCAACTTAAAGAAAGCGGCCGAAAGGGTATCCCTGGAGTCCAGAGGCTCAATCCGCATGAATGTCAAATTACATAAGTGCTAAGGTGAGAAGGTTGAAATGGATAAAGTACTTGCCAGTTTGATTGAGAATTTCGGTCCGCCGATTGACC  >  ACB122/891761‑892026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑