Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	924,303	T→G	27.0%	D4790A (GAC→GCC)	CJ019_00881 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	924,303	0	T	G	27.0%	75.8 / 5.9	63	D4790A (GAC→GCC)	CJ019_00881	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base G (6/13);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGT  >  ACB122/924173‑924431                                                                                                                                   |                                                                                                                                 ttGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCACGCTGCCGTCGGc                                                                                                                              >  4:188457/1‑135 (MQ=255) ttGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGc                                                                                                                              >  5:428749/1‑135 (MQ=255)  tgtgCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGc                                                                                                                              <  1:220107/134‑1 (MQ=255)     gCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGtt                                                                                                                           <  4:44415/134‑1 (MQ=255)     gCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGtt                                                                                                                           <  7:133757/134‑1 (MQ=255)     gCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGtt                                                                                                                           <  2:65795/134‑1 (MQ=255)      cGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGa                                                                                                                         <  5:221564/135‑1 (MQ=255)          tGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATcac                                                                                                                     <  1:276768/135‑1 (MQ=255)                            gCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTc                                                                                                   <  1:116969/135‑1 (MQ=255)                                 cATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGGCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcg                                                                                              <  8:13468/135‑1 (MQ=255)                                  aTCTGCAAGTACGAAACCCGTGCCCCGTACGGTACCGGCCGCGTTACTGACGATTCCCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCGGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcgg                                                                                             <  7:190986/135‑1 (MQ=255)                                  aTCAGCCAGTACGATACCCGGGCCCAGTACGGTACCGGCCGCGTTACTGCCGATTACCGTGGCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcgg                                                                                             <  3:319482/135‑1 (MQ=255)                                            aCGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGAGGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTcc                                                                                   <  6:397391/135‑1 (MQ=255)                                            aCGATACCCGGGCCCCGGACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCGGCGCCCAGGCCGCTGACCAGGCTGCCGGCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTcc                                                                                   <  8:175113/135‑1 (MQ=255)                                             cGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCCTGGCGCCGGCCTCGCCCCCGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCg                                                                                  >  2:90756/1‑135 (MQ=255)                                                   ccGTGCCCAGTACGGTACCGGCCGCGTTTATGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACCCTGCCGTCGGCGTTGATGACCACGTGGGTGAGTGGGTGGGGGGGGGTGAAGGACGGGGttt                                                                            >  8:436923/1‑135 (MQ=255)                                                    cGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGCCCACGCTGCCGGCGGCGTTGATAACCACGTGGGTGTGTGGGTCGGGCGGGGTGAAGGCCGGGGGTTg                                                                           >  8:197986/1‑135 (MQ=255)                                                        cccAGTACGGTACCGGCCGCGTTACTGACGATTTCCCTGGCGCCGGCCACGCCCAGGCCCCCGCCCACCCTGGCGGCGGGGTGTGTGCCCCCATGGGGGTGTTGGTGGGGCGGGGGTGATTCCGGGGGGTGCAgg                                                                       >  6:53098/1‑135 (MQ=255)                                                        cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCGGCCCACGCTGCCGGCGGCGGTGATCCCCACATGGGTGAGTGGGTCGGGGGGGGTGGAGTCCGGGGGTTGGAgg                                                                       >  7:390949/1‑135 (MQ=255)                                                        cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcggg                                                                                            <  3:220134/114‑1 (MQ=255)                                                        cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcggg                                                                                            >  4:220134/1‑114 (MQ=255)                                                        cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGGTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAgg                                                                       >  3:103758/1‑135 (MQ=255)                                                            gTACGGTACCGGCGGCGTTACTGACGATTACCGTGGCGCCGGCCGCGCGCTGGCCGCGGACCAGGGTGCCGGCGGCGTTTATCACCACTTGGGTGAGTGGGGCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAg                                                                   <  8:82696/135‑1 (MQ=255)                                                                ggTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCCCGCTGCCGTCGGCGTTGATCACCACTTGGGGGAGTGGGGCGGGCGGGGGGAAGTGCGGGGTTTGCAGGGTAGCCAc                                                               >  8:246766/1‑135 (MQ=255)                                                                   aCCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCGCGCCCAGGCCGCTGACCCCCCTGCCGGCCGCGTTGATCACCCCGTGGGTGAGTGGGTGGGGCGGGGGGAAGTCCGGGGGTTGGAGGGTAGCCACAGc                                                            >  4:14260/1‑135 (MQ=255)                                                                     cGGCCGGGTTACTCACGATAACCGTGGCGCCGGCCTCGACCAGGCCGGTGACCACGCTGCCGGCGGCGTTAATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCg                                                          <  4:389745/135‑1 (MQ=255)                                                                     cGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCCCCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCg                                                          >  8:61334/1‑135 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCCCGCTGCCGTCGGCGTTGATAACCACTTTGGTGAGTGGGTCGGGCGGGGTGAATTCCGGGGTTTGCAGGGTAGCCCCAGCCGACACg                                                     >  4:51059/1‑135 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGCCCACCCTGCCGGCGGCGTTGATCACCCCGTGGGGGAGTGGGGGGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACg                                                     >  7:221653/1‑135 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCCCGCTGCCGTCGGCGTTGATAACCACGTGGGGGAGGGGGGCGGGCGGGGGGGAGTCCGGGGGTTGGAGGGTTGCCCCGGCCgccccg                                                     >  1:141019/1‑130 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCCCGCTGCCGGCGGCGTTGATGCCCACTTGGGTGATTGGGTCGGGCGGGGTGTAGTCCGGGGGTTTCAGGGTAGCCCCAGCCGccacc                                                     >  4:289796/1‑134 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGtt                                                                                                                           <  5:216314/65‑1 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGtt                                                                                                                           >  6:216314/1‑65 (MQ=255)                                                                          gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGGTGATCACCACGTGGGTGAGTGGGTCGGGGGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCAATGCCGCCACg                                                     >  8:53039/1‑135 (MQ=255)                                                                              tACTGACGATTCCCGTGGCGCCGGCCTCGCCCAGGCCGCGGACCACGCTGCCGGCGGCGTTGAGCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgc                                                 <  8:390949/135‑1 (MQ=255)                                                                                   aCGATTAGCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCAGGCTGCCGTCGGGGTCGATCACCACTTGGGTGAGTGGGTCGGGCGTGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgccggcc                                            <  7:322975/135‑1 (MQ=255)                                                                                    cGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCGGACCACGCTGCCGGCGGCGTTGATCACCACTTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgccggccg                                           <  4:118280/135‑1 (MQ=255)                                                                                        tACCGGGGCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGGGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATc                                       <  3:51059/135‑1 (MQ=255)                                                                                         aCCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCg                                      >  1:91593/1‑135 (MQ=255)                                                                                                cgcCGGCCTCGCCCAGGCCGCTGCCCACGCTGCCGTCGGCGGTGATCACCCCTTGGGTGAGTGGGGCGGGCGGGGTGCAGTCCGGGGGTTGCAGGGTAGCCCCTGCCGACACGTTGCCGGCCGCATCGGCCgggg                               >  5:351982/1‑135 (MQ=255)                                                                                                cgcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGa                                                                                                                         <  8:285784/45‑1 (MQ=255)                                                                                                cgcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGa                                                                                                                         >  7:285784/1‑45 (MQ=255)                                                                                                cgcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCCTCGGCCTggg                               >  8:114274/1‑135 (MQ=255)                                                                                                cgcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACTGCCGACCCGTTGCCGGCCGCCTCGGCCgggg                               >  3:36043/1‑135 (MQ=255)                                                                                                  ccGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGt                              >  2:330743/1‑134 (MQ=255)                                                                                                            cccggCCGCTGCCCACGGTGCGGTCGGGGTTGATCACCACTTGGGTGTGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                   <  3:449188/132‑1 (MQ=255)                                                                                                            cccggCCGCTCCCCACGGGGCGGGCGGCGTGTATCACCCCTTGGGTGTGGGGGGCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                   <  5:53098/132‑1 (MQ=255)                                                                                                            cccggCCGCGGCCCACGCTGCCGGCGGCGTTGAGCACCCCTTGGGTGCGTGGGGCGGGCGGGGGGAAGGCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                   <  3:291849/132‑1 (MQ=255)                                                                                                            ccAGGCCGCTGACCACGCTGCCGTCGGCTTTGAGCACCACTGGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTGGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                   <  1:354805/135‑1 (MQ=255)                                                                                                                 ccGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg              <  1:90756/135‑1 (MQ=255)                                                                                                                  cGCTGACCAGGCGGCCGGCGGCGTTGATCACCCCTTGGGTGAGGGGGGCGGGCGGGGGGAAGTCCGGGGGTTGCAGGGGAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCgg             <  5:82050/135‑1 (MQ=255)                                                                                                                  cGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGCCACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCgg             >  4:104944/1‑135 (MQ=255)                                                                                                                   gCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGCGCAGCTCGGc            >  6:6243/1‑135 (MQ=255)                                                                                                                   gCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGc            >  5:264411/1‑135 (MQ=255)                                                                                                                      gcccccgcgGCCGTCGGCGTTGCTCCCCACTTGGGTGAGTGGTGCGGGCGGGGTGAAGGCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGGGAAGCTGAGCAGCTCGGCGtt         <  5:153432/130‑1 (MQ=255)                                                                                                                      gACCAGGGTGCCGGCGGCTTGGATCACCGCTTGGGGGAGGGGGGCGGGCGGGGTGAAGGCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGtt         <  5:381332/135‑1 (MQ=255)                                                                                                                      gACCACGCGGCCGTGGGCGTTGATCACCTCTTGGGGGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGtt         <  3:188457/135‑1 (MQ=255)                                                                                                                       aCCACGCTGCCGTCGGCGTTGATCACCCCTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGGCTGGGTGAAGCTGCGCAGCTCGGCGTTg        >  6:350272/1‑135 (MQ=255)                                                                                                                       aCCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGCAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGGGAAGCTGCGCAGCTCGGCGTTg        >  8:335020/1‑135 (MQ=255)                                                                                                                        ccACGCTGCCGGCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGc       <  8:384626/135‑1 (MQ=255)                                                                                                                            gcggCCGTCGGCGTTCAGAACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAg   <  7:424680/132‑1 (MQ=255)                                                                                                                            gCTGCCGGGGGCGTTCATCCCCACTGGGGTGAGTGGGGCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAg   <  1:438277/135‑1 (MQ=255)                                                                                                                             cTGCCGGCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGt  <  3:296597/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 TTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGT  >  ACB122/924173‑924431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑