Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 930,475 T→G 14.6% T2733P (ACC→CCC)  CJ019_00881 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122930,4750TG14.6% 146.5 / 4.2 81T2733P (ACC→CCC) CJ019_00881hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/43);  new base G (10/4);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACCACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGCACCCACCACG  >  ACB122/930343‑930608
                                                                                                                                     |                                                                                                                                     
ccaccaCGACACTGCCCAGTACAGGGGGGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGa                                                                                                                                     <  6:124737/135‑1 (MQ=255)
        acacTGCCCTGTACCGGGGTGCCTTGGGCGGCCAGCACACGGAGGGGGCTGCCTGGTGCGGCGGGGCCGCTGACGACGGTACCGTCGCCACTGATCGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGt                                                                                                                             <  5:309209/135‑1 (MQ=255)
         cacTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTg                                                                                                                            <  1:54569/135‑1 (MQ=255)
               ccAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGCCGGGACCGTCGCCCCGGGTTGGCCGCAGCCGTGGCGCCACCCGCGC‑GGTGGTGTGTGGTggggcgc                                                                                                                      >  4:6953/1‑134 (MQ=16)
                 aGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGAcc                                                                                                                    >  7:371531/1‑135 (MQ=255)
                   tACAGTGGTGCCATCGGCGGCCAGCACACGGACGGGGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTg                                                                                                                  <  3:140982/135‑1 (MQ=255)
                   tACAGGGGCGCCATCGGCGGCCAGCACACGGACGTTGCGGCGTGCTTCGGCGCGGCCGCTGACTACGGTACCGTCGCCACTGATTGCCAGATCGCGTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTg                                                                                                                  <  8:105251/135‑1 (MQ=37)
                      aGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCTGCTTGCCAGATCGCTTGGCGCCCCAGGCGC‑GGTGATGTCTGGTGCGGCGCCCTGCAc                                                                                                               >  6:229523/1‑135 (MQ=255)
                      aGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGCTTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCAc                                                                                                               >  2:290395/1‑135 (MQ=255)
                      aGTGGTGCCAGCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCGGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCAc                                                                                                               <  8:453179/135‑1 (MQ=255)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGCCGGGACCGTCGCCCCAGATTGCCAGATCGCTTGGCGCCCCCGGCGG‑CGGGGTGTATGGTTCGGCGGCCTGCACCGGcc                                                                                                          >  1:85537/1‑134 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTTCCGGCGCCACTGATTTCCCGATAGCGTGGCGCCCCACGCGC‑GGGGATGTCGGGAGCCGGGACCTGCACCGGcc                                                                                                          >  7:53427/1‑134 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCCCTGATTGCCAGATCGCGTGGCGCCCCCGGCGC‑GGTGATGTCTGGTGCGGCGCCCTGCACCGGcc                                                                                                          >  4:48040/1‑134 (MQ=37)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCGACGGTACCGTCGCCACTGATTGCCAGATAGCTTGGCGCCACAGGCGC‑GGTGCAGTCTGGTGCGGCGCCCTGCACCGGCa                                                                                                          >  3:357736/1‑135 (MQ=37)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGCCCCCGGGACCCGCGCCCCTGCTTTCCAGATCGCTTGGGCCCCCCCGCGC‑GGGGGTGTGGGGTGCGGCGccccgcccccgcc                                                                                                          >  1:188954/1‑134 (MQ=12)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTTCCGTCGCCCCTGCTTGGCAGATAGCTTGGCGCCCCAGGCGC‑GGGGGTGTGTGGTTCGGCGCccggcaccggca                                                                                                          >  1:48449/1‑135 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCCCTGATCGCCAGAGCGCTTGGCGCCCCAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCCGcc                                                                                                          >  2:339266/1‑134 (MQ=37)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACAGATTGCCAGCTCGCATGGCGCCCCCCGCGC‑GGTGCTGTCTGGTGCGGCGaccggcaccggcc                                                                                                          >  5:67848/1‑134 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCCTCGCCACTGCTTGGCAGCTCGCTTGGCGCCCCAGGCGC‑GGGGGTGTGTGGTGCGGCGGCCGGCACCCGcc                                                                                                          >  5:190915/1‑134 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGGACCGTCGCCCCTGATTGCCACATCGCTTGGCGCCACAGGGGCGGGGGAGTTATGTTGCGGCGCCCTGCACCGGc                                                                                                           >  8:128201/1‑135 (MQ=25)
                           tgCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACCGTTCCGTCGCCCCGGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGGTGTATGGTGCGGCGGCCAGCCCCCGcc                                                                                                          >  7:32320/1‑134 (MQ=25)
                                tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGGCCATTATTGGCCGATCACTTTGCGCCCCAGGCCC‑GGGGAAGACAGGGGCCGCCCCCTGCACCGGCAac                                                                                                        >  2:8257/1‑132 (MQ=16)
                                tCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAacgac                                                                                                     >  8:63414/1‑135 (MQ=255)
                                tCGGCGGCCAGCACACGGACGGTGCTGACTGCTTCGGCGAGGCCGCTTACCACGGTACCGTCGCCACTGAATTCCAGATAGGATGGCGCCACGGGCGC‑GGGGATGTCTGGTGCCGCGACCATCACCGGCaaacg                                                                                                      >  8:151137/1‑131 (MQ=25)
                                   gcggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTTCCGTCGCCACTGCTTGGCAGATCGCTTGGCGCCACAGGCGC‑GGGGATGTCTGGTGCGGCGACCTGCACCGGCCACGACGAg                                                                                                  >  8:23446/1‑135 (MQ=255)
                                   gcggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCCCCGGCAACGCCGAg                                                                                                  >  4:286132/1‑135 (MQ=255)
                                     ggcCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCCCAGGCGC‑GGTGCTGTCTGGTGCGGCGCCCTGCACCGGCCACGACGAGGt                                                                                                >  4:20065/1‑135 (MQ=255)
                                                        ctgcctgcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGGGATGTCTGGTGCGGCGACCTGCACCGGCCACCACGCGGTGCCGTTGTTGTCGCTCGcc                                                                             >  1:283221/1‑135 (MQ=255)
                                                        ctgcctgcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATAGCTTGGCCCCACAGGCGC‑GGTGATGTCAGGTTCGGCGACCAGCACCGGCAACCACGATGTGCCCTTGTTGTCGATcccc                                                                             >  6:121528/1‑135 (MQ=255)
                                                        ctgccggcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGcc                                                                             <  4:301755/135‑1 (MQ=255)
                                                              gcTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCCCCCGCGC‑GGTGATGTCTGGTGCGGCGCCCTGCCCCGGCAACGCCGAGGTGCCGGTGGTGTCGAACGCCACCCCg                                                                       >  4:375711/1‑135 (MQ=255)
                                                              gcTCCGGCGCGGCCGCGGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGGGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACg                                                                       <  4:305628/135‑1 (MQ=255)
                                                                 tCGGCGCGGCCGCTGACGCCGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGCTGTCTGGTGCGGCGACCTGCACCGGCAACGACGCGGTGCCGTTGTTGTCGATCGCCACCACGt                                                                      >  8:368154/1‑133 (MQ=255)
                                                                   ggCGCGGCCGCTGCCGCCGGTACCGTCGCCACTGATTGCCCGCTCGCTTGGCGCCACAGGCGC‑GGTGCTGTCTGGTGCGGCGGCCTGCCCCGGCCACGCCGAGGTGCCGGTGTTGGCGCTCGCCCCCCCGTCCac                                                                  >  8:152939/1‑134 (MQ=255)
                                                                   ggCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAg                                                                  <  1:290395/135‑1 (MQ=255)
                                                                     cggggCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACTGGCGC‑GGTGATGTCTGGTGCGGCGACCGGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCa                                                                <  2:446716/132‑1 (MQ=255)
                                                                     cgcgGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCAGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCa                                                                <  3:210574/135‑1 (MQ=255)
                                                                          ccGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                           <  1:83558/135‑1 (MQ=255)
                                                                          ccGCTGACGACGGTACCGGCGCCACTGATTGCCAGCTCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                           <  4:383885/135‑1 (MQ=255)
                                                                          ccGCGGACGACGGTACCGTCGCCACTGATTGCCAGAGCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCg                                                           <  5:324559/135‑1 (MQ=255)
                                                                           cgcgGACGACGGTACCGTCGCCAGTGAGTGCCAGATCGCGGGGCGCCACTGCCGC‑GGGGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGGCGATCGCCACCACGTCCAGCAACTCGc                                                          <  4:405554/135‑1 (MQ=255)
                                                                           cGCTGACGACGGTACCGGCGCCACTGATTGCCATATCGCTTTGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGAGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGc                                                          <  8:385796/135‑1 (MQ=255)
                                                                                acgacgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTg                                                     <  3:20065/135‑1 (MQ=255)
                                                                                    gggTACCGTCGCCACTGAGGGCCTGATCGGTTGGCGCCACCGGGGG‑GGGGATGCCGGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  1:247425/134‑1 (MQ=255)
                                                                                    cgGTCCCGTCGCCACTGATTGCCCGATCGCTTGGCGCCACCGGCGC‑GTTGATGTCTGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  1:212880/135‑1 (MQ=255)
                                                                                    cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  5:121528/135‑1 (MQ=255)
                                                                                    cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  2:51982/135‑1 (MQ=255)
                                                                                    cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  3:236676/135‑1 (MQ=255)
                                                                                    cgGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCt                                                 <  8:12585/135‑1 (MQ=255)
                                                                                     ggTAGCGCCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                                <  7:202330/135‑1 (MQ=255)
                                                                                     ggTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                                <  4:185316/135‑1 (MQ=255)
                                                                                      gTACCGTCGCCACTGATTGCCAGAGCGCTTGGCGCCACAGGCGC‑GGTGATGGCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTgg                                               <  3:395145/135‑1 (MQ=255)
                                                                                       tACCGTCGCCCCTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGGGACCTGCACCGGCAACGCCGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTggg                                              >  8:260788/1‑135 (MQ=255)
                                                                                       tACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                                >  6:201975/1‑133 (MQ=255)
                                                                                       tACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTg                                                <  5:201975/133‑1 (MQ=255)
                                                                                         ccGTCGCCACTGATTGCCAGATCGCTTGGCGCCACTGGCTC‑GGTGATGTCTGGCGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCAttggccttggcc                                            <  3:378551/135‑1 (MQ=255)
                                                                                           gTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGGCGATCGCCACCACGTCCAGCAACTCTCCATTGGCCTGGGCCgg                                          <  4:216842/135‑1 (MQ=255)
                                                                                              gCCACTGATTTCCAGATCGCTTGGCGCCACAGGGGC‑GGTTATGTCTGGTGCGGCGACCTGCACCGGCAACGACGATGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCTCCATTGGCCTGGGCCGGtat                                       <  3:110209/135‑1 (MQ=255)
                                                                                              gCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGtat                                       <  8:32320/135‑1 (MQ=255)
                                                                                              gCCACTGATTGCCAGATCGCGTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGtat                                       <  4:361740/135‑1 (MQ=255)
                                                                                              gCCACTGATTGCCAGACCGGTGGGCGCCACCGGCGC‑GGTGATGTCTGGGGCGGCGACCGGCCCCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGtat                                       <  3:6953/135‑1 (MQ=255)
                                                                                               ccACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGtat                                       <  3:166343/134‑1 (MQ=255)
                                                                                                 acggATTGCCAGAGCGCTGGGCGCCACAGGCGC‑GGGGAGGGCTGGTGAGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACGCGCCAGTGGCCTGGGCCGGTATAAg                                    <  6:67848/132‑1 (MQ=255)
                                                                                                 aCTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAg                                    <  8:371531/135‑1 (MQ=255)
                                                                                                 aCTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAg                                    >  3:140610/1‑135 (MQ=255)
                                                                                                   tGATTGCCAGATCGCTTGGCGCCACCGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGATGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGca                                  <  5:370817/135‑1 (MQ=255)
                                                                                                   tGATGGCCAGTGCGCGTGGCGCCCCCGGCGG‑GGTGATGTGGGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGca                                  <  1:225451/135‑1 (MQ=255)
                                                                                                      ttGCCAGAGCGCTTGGCGCCACCGGCGC‑GGTGATGGCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGcacca                               <  7:152939/135‑1 (MQ=255)
                                                                                                            gATCGGTGGGCGCCACCGGCGC‑GGTGATGTCTGGGGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCT‑GGCCGGTATAAGCACCACGGTGaa                        <  1:445799/135‑1 (MQ=255)
                                                                                                             ctCGCGTGGCGCCACCGGCGC‑GGTGAGGGCGGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGaa                        <  1:256268/134‑1 (MQ=255)
                                                                                                             aTCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGaa                        >  3:217867/1‑135 (MQ=255)
                                                                                                             aTCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGaa                        <  3:227400/135‑1 (MQ=255)
                                                                                                                gCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGt                     >  1:250966/1‑135 (MQ=255)
                                                                                                                gCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGCCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGt                     >  3:183154/1‑135 (MQ=255)
                                                                                                                  ttGGCGCCACAGGCGC‑GGTGATGGCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGc                   <  6:227273/135‑1 (MQ=255)
                                                                                                                       gcCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTg                                                     >  7:429517/1‑96 (MQ=255)
                                                                                                                       gcCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTg                                                     <  8:429517/96‑1 (MQ=255)
                                                                                                                         caccGGCGC‑GGGTCTGTCGGGGGCGGCGACCTGCCCCGGCAACGACGCGGGGCCGTGGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGGGCCCTCGGc            <  6:320126/135‑1 (MQ=255)
                                                                                                                             tgggggggTGATGGCTGGTGCGGCGACCTGCACCGGCAACGACGAGGGGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGcacc         <  8:16224/129‑1 (MQ=255)
                                                                                                                              gcgc‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGcaccca       >  4:305138/1‑135 (MQ=255)
                                                                                                                                    gTGATGTCTGGGGCGGCGACCTGCACCGGCAACGACGAAGTGCCGTTGTTGTCGAGCCCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAACCACCACGGTGAAGGGGCCCTCGGCACCCACCACg  <  2:85537/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CCACCACGACACTGCCCAGTACAGTGGTGCCATCGGCGGCCAGCACACGGACGGTGCTGCCTGCTTCGGCGCGGCCGCTGACGACGGTACCGTCGCCACTGATTGCCAGATCGCTTGGCGCCACAGGCGC‑GGTGATGTCTGGTGCGGCGACCTGCACCGGCAACGACGAGGTGCCGTTGTTGTCGATCGCCACCACGTCCAGCAACTCGCCATTGGCCTGGGCCGGTATAAGCACCACGGTGAAGGTGCCCTCGGCACCCACCACG  >  ACB122/930343‑930608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑