Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 932,023 T→G 14.7% T2217P (ACC→CCC)  CJ019_00881 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122932,0230TG14.7% 134.7 / 3.2 73T2217P (ACC→CCC) CJ019_00881hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/46);  new base G (11/2);  total (25/48)
Fisher's exact test for biased strand distribution p-value = 6.12e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTACCACCACACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGG  >  ACB122/931889‑932157
                                                                                                                                       |                                                                                                                                      
aCCTACCACCACACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGGCGCTTGGGCTGGCTGGGGTCGT‑GGt                                                                                                                                        <  2:246176/135‑1 (MQ=255)
 ccTACCACCACACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGtt                                                                                                                                       <  1:278918/135‑1 (MQ=255)
  cTACCACCACACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTg                                                                                                                                      <  1:292382/135‑1 (MQ=255)
     ccaccaCACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCg                                                                                                                                   <  4:71584/135‑1 (MQ=255)
         cacaCTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGGGGGGGGGCGGGGGTGGTGGGTGGCCgggg                                                                                                                                >  7:364289/1‑134 (MQ=255)
          acacTGCCGACCAGTGTGCCGTTGGCTTCGTGTACTTCCACTCGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGttt                                                                                                                              <  1:214120/135‑1 (MQ=255)
            acTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGGTGCCGGGAtttg                                                                                                                            >  4:215464/1‑134 (MQ=255)
            acTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGCtttg                                                                                                                            >  7:157090/1‑134 (MQ=255)
               gCCGACCAGTGTGCCGTTGGCATCGTGGACTTCCACACGGCTGCCCGTCGGGGCGGTACCAGTGATGCTGGCGCCGGTCGCCGCCACCCCCAGGGCGCTTGGGCTGGCTGGGGGCGT‑GGGTGGCGGGGTTTCcta                                                                                                                         >  4:28758/1‑135 (MQ=255)
                 cgcccAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCCGCCACCACCCGGTCGCGTGGGGGGGCGGGGGGCGG‑GGGTGGCGGGGTTTGATAtt                                                                                                                       >  6:302353/4‑135 (MQ=255)
                 cGACCAGTGTGCCGTTTGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATAtt                                                                                                                       <  5:290403/135‑1 (MQ=255)
                 cGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGattttatttt                                                                                                                       >  8:154497/1‑135 (MQ=255)
                 cGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATAtt                                                                                                                       <  4:390410/135‑1 (MQ=255)
                  gACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTg                                                                                                                      <  7:160138/135‑1 (MQ=255)
                  gACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTg                                                                                                                      <  4:112986/135‑1 (MQ=255)
                   aCCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCCGGATTTCataat                                                                                                                       >  8:3857/1‑131 (MQ=255)
                      aGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGAGCTGGCTGGGGGCGT‑GGGGTGCGGGGTTTGATATTGGAgg                                                                                                                  >  2:361639/1‑135 (MQ=38)
                           gCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGGTTGCGGGGTTTTATATTTGAggggg                                                                                                               >  2:362064/1‑131 (MQ=255)
                           gCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGGCGG‑GGGTGGCGGGTTTTGATATTGGAgggggtc                                                                                                             >  6:425538/1‑131 (MQ=255)
                           gCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGTCGT‑GGGTGGCGGGCTTTCATATTGGAggggg                                                                                                               >  3:152198/1‑131 (MQ=255)
                                  gCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGGTGGGTGGGGTCGT‑GGGTGGCGGGGTTTGATATTTGCGGGGTTGAGAGGCg                                                                                                      >  4:186498/1‑135 (MQ=255)
                                       gtgtACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGcc                                                                                                 <  4:274965/135‑1 (MQ=255)
                                           aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                             <  1:262597/135‑1 (MQ=255)
                                           aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                             <  8:290963/135‑1 (MQ=255)
                                           aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGG‑GGTGGCCGGGGTTTAATATTGCAGGGCGTCCGAGGCCTTGCCGcgc                                                                                             >  2:105287/1‑133 (MQ=255)
                                           aCTTCCACACGGCTGCCCGTCGGGGCGGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑TGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                             <  6:209339/135‑1 (MQ=255)
                                           aCTTCCACACGGCTGCCCGGCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                             <  6:217033/135‑1 (MQ=255)
                                            cTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTGATATTGCAGGGCTTCAGAGGCGTTGGCGCTc                                                                                             >  4:99481/1‑134 (MQ=255)
                                             ttCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCg                                                                                            <  3:411094/134‑1 (MQ=255)
                                               ccACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGGTGGCGGGGGTCGT‑GGTTGGCGGGATTTGATATTGGCAGGCGTCAGAGGCGTTTgcgcgcgcct                                                                                         >  7:35521/1‑133 (MQ=255)
                                                cacaCGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATc                                                                                        <  1:434122/135‑1 (MQ=255)
                                                cacaCGGCTGCCCGGCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATc                                                                                        <  5:306087/135‑1 (MQ=255)
                                                 acacGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATc                                                                                        <  4:188913/134‑1 (MQ=255)
                                                 acacGGCTGCCCGTCGGGGCTGTAACAGGGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTTTCTGGGGTCGT‑GGGTGCCGGGATTTGATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCg                                                                                       <  6:186080/135‑1 (MQ=255)
                                                  cacGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCgg                                                                                      >  4:184135/1‑135 (MQ=255)
                                                  cacGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCgg                                                                                      <  6:169131/135‑1 (MQ=255)
                                                       cTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGc                                                                                 <  3:431281/135‑1 (MQ=255)
                                                            cGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGGTTTGATATTGCAGGGCTTCAGAGGCGTTGCCGCGCGCCTCGGTCTGCACCAg                                                                            >  8:41398/1‑135 (MQ=255)
                                                             gTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTTCAGGGCTTCAGGGGCGTTGCCGCTCGCATCGGTGTGCACCAgg                                                                           >  4:135208/1‑135 (MQ=255)
                                                                        aCCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCGGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGc                                                                  <  2:396343/133‑1 (MQ=255)
                                                                          cAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGGTTTCAAATTTCAGGGCTTCAGAGGCGTTTCCGCTCGCATCGGTCTGCACCACGCTCCAATGcgacc                                                              >  6:67704/1‑131 (MQ=255)
                                                                               aTGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGGCGGGGGTTGATATTGGAGGGCTTCATAGGGGTTGCCCCGCGGCTCGGGGTGGACCAGGCGGAATTGCTCGCCCGGt                                                         >  2:328681/1‑135 (MQ=255)
                                                                                tGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGtt                                                        <  3:175629/135‑1 (MQ=255)
                                                                                tGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGtt                                                        <  4:277530/135‑1 (MQ=255)
                                                                                tGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGtt                                                        <  5:425538/135‑1 (MQ=255)
                                                                                      cgcCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTgcgc                                                    <  7:355600/133‑1 (MQ=255)
                                                                                      cgcCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTgcgc                                                    <  3:407597/133‑1 (MQ=255)
                                                                                       gcCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCGGGGGTCGT‑GGGTGGCGGGCTTTAATATTGCGGGGCGTCACATGCGTTGCCGCTGGCATAGGGCTTGACCACGCGCAATTTCTACCCCGGTTTCGCGGc                                                 >  4:193256/1‑135 (MQ=255)
                                                                                          gTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGGTGGCGGGGTTTGATTTTTCAGGGCGTCAGAGGCGTTGCCGCGCGCCTCGGTGTGCCCCCGGCGCAATTTCTCGCCCCGGTGGGCgggggg                                              >  6:159970/1‑131 (MQ=255)
                                                                                              gCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGt                                           <  5:166062/134‑1 (MQ=255)
                                                                                              gCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGGTGCCGGGGTTTGATATTTCAGGGCGTCAGAGGCGTTTCCGCGCGCCTCGGTCTGCCCCACGCTCAATTGCTCGCCCGGTTGCGCGGCGgggggg                                          >  2:301779/1‑133 (MQ=255)
                                                                                               cAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGGTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGa                                         <  6:437737/135‑1 (MQ=255)
                                                                                                        ccaGGTCGCTTGGGCTGGCGGGGGGCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCa                                <  3:28758/135‑1 (MQ=255)
                                                                                                            gTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCaagaa                            <  2:74484/135‑1 (MQ=255)
                                                                                                                     gctggctgGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGtt                   <  7:271885/135‑1 (MQ=255)
                                                                                                                      ctggctggGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTg                  <  1:83244/135‑1 (MQ=255)
                                                                                                                            ggggTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTAGCCCGGTTGCGCGGCTGGGGTGAGGTCGCCCAAGCAGGTTCCGTTGGCGCCg            >  8:25316/1‑135 (MQ=255)
                                                                                                                                  gT‑GGTTTCCGGGATTTAATATTTCAGGGCTTCAGAGGCGTTTCCCCTCGCAAACGTCTTCACCAGGCTCAATATCAAGCCACGTTTCGCGGCTGGGGTTAGGTGGACCAAAAAGGTTACGTTGGGGCCCACagtg      >  2:240111/1‑135 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGTGCCGACtgtg      <  7:230347/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  5:279930/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  1:301779/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  7:363220/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCAGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  8:223367/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCACATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  1:120954/135‑1 (MQ=255)
                                                                                                                                  gT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTGGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  8:35521/135‑1 (MQ=255)
                                                                                                                                  gT‑GGGTGCCGGGATTTCATATCGCAGGGCTTCAGAGGCGTGGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCGCGCCCGGTGGCGCGGCGGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  1:439967/135‑1 (MQ=37)
                                                                                                                                   t‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  5:379965/134‑1 (MQ=255)
                                                                                                                                   t‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  5:236556/134‑1 (MQ=255)
                                                                                                                                   t‑GGTTGCAGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCTATTGCTCGCCGGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  1:8457/134‑1 (MQ=255)
                                                                                                                                   ggggTTGCCGGGATTTCATTTTGCAGGGCTTCAGAGGCGTGGCCGCTCGCATCGGGCTGCACCAGGCTCATTTGCTCGCCCGGTTGCGCGGCTGGGGGGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  1:406227/133‑1 (MQ=255)
                                                                                                                                   ggggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg      <  2:453096/133‑1 (MQ=255)
                                                                                                                                     ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGGCTGTGGc    <  5:67704/135‑1 (MQ=255)
                                                                                                                                     ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGc    <  3:186498/135‑1 (MQ=255)
                                                                                                                                     ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGc    <  1:354944/135‑1 (MQ=255)
                                                                                                                                     ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTGGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCGGGGGTGAGGGCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGc    <  8:71954/135‑1 (MQ=255)
                                                                                                                                       ttGCCGGGATTTAATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATAGGTCTGCACCAGGCTCAATTTCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCgg  >  7:330799/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ACCTACCACCACACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGT‑GGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGG  >  ACB122/931889‑932157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑