Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	968,666	C→G	5.4%	G175R (GGC→CGC)	suhB ←	Inositol‑1‑monophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	968,666	0	C	G	5.4%	232.8 / 5.5	74	G175R (GGC→CGC)	suhB	Inositol‑1‑monophosphatase
Reads supporting (aligned to +/- strand):  ref base C (41/29);  new base G (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CCATGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCGCCACGGCCACGGCTG  >  ACB122/968534‑968799                                                                                                                                     |                                                                                                                                      ccATGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCACGGCGCGGCACATGCCCa                                                                                                                                     >  6:330319/1‑135 (MQ=255) ccATGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCa                                                                                                                                     >  2:461165/1‑135 (MQ=255) ccATGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCa                                                                                                                                     <  4:350655/135‑1 (MQ=255)   aTGTCCCATTCTTACTGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAgg                                                                                                                                   <  1:284935/135‑1 (MQ=255)   aTGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAgg                                                                                                                                   <  2:381821/135‑1 (MQ=255)       cccATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGt                                                                                                                               >  3:420992/1‑135 (MQ=255)          aTTCGGACAGGCCCGACTCCCAGACGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGt                                                                                                                            <  4:21115/135‑1 (MQ=255)              ggccAGGCCCGACTCCCAGACGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCAt                                                                                                                        <  5:426221/132‑1 (MQ=255)              ggACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCGCAGGTAGTTGTCCAt                                                                                                                        >  4:127465/1‑135 (MQ=255)              ggACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCGCAGGTAGTTGTCCAt                                                                                                                        >  4:120965/1‑135 (MQ=255)              ggACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCAt                                                                                                                        >  6:227009/1‑135 (MQ=255)              ggACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCAt                                                                                                                        >  4:283399/1‑135 (MQ=255)              ggACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCAt                                                                                                                        >  3:44603/1‑135 (MQ=255)                aCAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGt                                                                                                                      <  7:211293/135‑1 (MQ=255)                   ggCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCg                                                                                                                   >  6:436630/1‑135 (MQ=255)                   ggCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCg                                                                                                                   >  5:308481/1‑135 (MQ=255)                   ggCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATG‑CCAGGTAGTTGTCCATGTCCg                                                                                                                   >  8:234820/1‑134 (MQ=255)                      ccGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCa                                                                                                                >  8:16997/1‑135 (MQ=255)                       cGACTCCCAGAAGGCGTCGAAGCGGCCAGCCGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGccct                                                                                                               >  1:175195/1‑133 (MQ=255)                       cGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCAt                                                                                                               >  6:239381/1‑135 (MQ=255)                        gACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATc                                                                                                              >  6:29109/1‑135 (MQ=255)                        gACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATc                                                                                                              <  3:464659/135‑1 (MQ=255)                        gACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATc                                                                                                              >  4:31134/1‑135 (MQ=255)                         aCTCCCTGACGGCGGCCACGCGGCCAGCAGCCACGTAGGACAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCt                                                                                                             <  2:194691/135‑1 (MQ=255)                         aCTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCt                                                                                                             <  7:43932/135‑1 (MQ=255)                            cccAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGc                                                                                                          >  5:790/1‑135 (MQ=255)                              cagccggCGTCGAAGCGGCCTGAAGCCACGTCGGCCAGGTCCTGGGTGGCGGAGCCGGCGCGGCGGATGCCGGCGGGCTGGCCGGGCAAGGCGCTGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCg                                                                                                        <  7:236152/130‑1 (MQ=255)                                 aaGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCa                                                                                                     >  1:69294/1‑135 (MQ=255)                                 aaGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCa                                                                                                     >  8:330567/1‑135 (MQ=255)                                 aaGGCGTCGAAGCGGCCAGCAGCCACGTAGGACAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCa                                                                                                     >  1:194691/1‑135 (MQ=255)                                      gTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGaa                                                                                                >  2:128340/1‑135 (MQ=255)                                        cGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACg                                                                                              >  4:85589/1‑135 (MQ=255)                                        cGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACg                                                                                              >  6:464583/1‑135 (MQ=255)                                        cGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGGAGTAGACCATGTCCGACATCAGGCCGTCACGGAACg                                                                                              >  2:453222/1‑135 (MQ=255)                                          aaGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACgg                                                                                             >  1:42282/1‑134 (MQ=255)                                           cgCGGCCAGCAGCCCCGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGa                                                                                           <  2:425341/134‑1 (MQ=255)                                           aGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGa                                                                                           <  3:160513/135‑1 (MQ=255)                                             cGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAg                                                                                         <  8:160782/135‑1 (MQ=255)                                                ccAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCg                                                                                      <  1:21216/135‑1 (MQ=255)                                                       ccACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCGCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCca                                                                               <  3:120965/135‑1 (MQ=255)                                                       ccACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCGCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCca                                                                               <  3:127465/135‑1 (MQ=255)                                                          cGTAGGCCAGGTCCAGGCGGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCcagca                                                                            <  5:406677/135‑1 (MQ=255)                                                          cGTAGGCCAGGCCCAGGCGGGCGGAGCCGGCGCGGCGGATGGCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACTGAACGGGAAGCCGGTGCCcagca                                                                            <  3:275092/135‑1 (MQ=255)                                                            tcggccagggccagGCGGGGGGAGCCGGCGCGGCGGATGGCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGGTGTCCATGTCCGCCATCTGGCCGGCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  2:175195/133‑1 (MQ=255)                                                            tAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  8:37819/135‑1 (MQ=255)                                                            tAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  4:418624/135‑1 (MQ=255)                                                            tAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  4:428724/135‑1 (MQ=255)                                                            tAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  2:108961/135‑1 (MQ=255)                                                            tAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGGAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAgg                                                                          <  2:78466/135‑1 (MQ=255)                                                                    gTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCtt                                                                   >  6:189305/1‑134 (MQ=255)                                                                       cAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGACCGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAg                                                               >  8:194392/1‑135 (MQ=255)                                                                       cAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAg                                                               >  1:324061/1‑135 (MQ=255)                                                                       cAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTACTTGTCCATGTCAGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGACCAGCAGGCCGCCTTCCAg                                                               >  1:26031/1‑135 (MQ=255)                                                                             ggCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTtgt                                                         <  7:330567/135‑1 (MQ=255)                                                                                ggAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTgcg                                                      >  1:285917/1‑135 (MQ=255)                                                                                 gAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTgcgc                                                     >  7:172194/1‑135 (MQ=255)                                                                                 gAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTgcgc                                                     >  7:76029/1‑135 (MQ=255)                                                                                  aGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTgcg                                                      >  4:229330/1‑133 (MQ=255)                                                                                  aGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACAGGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAAGGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGAgcgct                                                    >  7:346572/1‑134 (MQ=255)                                                                                       gcgcggcgGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCCCGGCACGGGACGCCGGTGCCCCGCAGGGCGCCGTCCCGGCGTGTGCGCGCGCTg                                               >  2:226718/1‑135 (MQ=255)                                                                                           ggcggATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGAcgc                                           >  6:153337/1‑135 (MQ=255)                                                                                                 tGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAggc                                     <  3:45887/135‑1 (MQ=255)                                                                                                      gcggTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAggcggcgg                                <  3:283399/135‑1 (MQ=255)                                                                                                               ccGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAg                        >  2:455710/1‑134 (MQ=255)                                                                                                               ccGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGt                       >  1:101917/1‑135 (MQ=255)                                                                                                                 ggTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTg                     >  3:418933/1‑135 (MQ=255)                                                                                                                    cAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGgc                  <  2:350742/135‑1 (MQ=255)                                                                                                                    cAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGgc                  <  3:31134/135‑1 (MQ=255)                                                                                                                                cATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGa                                                                                           >  7:166584/1‑50 (MQ=255)                                                                                                                                cATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGa                                                                                           <  8:166584/50‑1 (MQ=255)                                                                                                                                cATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCgccacggccacg      >  4:435394/1‑135 (MQ=255)                                                                                                                                cATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCgccacggccacg      >  8:80465/1‑135 (MQ=255)                                                                                                                                cATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTAGGGCgccacggccacg      >  4:33205/1‑135 (MQ=255)                                                                                                                                 aTGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCGccacggccacgg     <  4:380162/135‑1 (MQ=255)                                                                                                                                    cccAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCGCCACGGCCACGGCTg  >  2:86023/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                      CCATGTCCCATTCGGACAGGCCCGACTCCCAGAAGGCGTCGAAGCGGCCAGCAGCCACGTAGGCCAGGTCCAGGCTGGCGGAGCCGGCGCGGCGGATGCCGGCGGTCTGGCCGGTCAAGGCGCGGAACATGCCCAGGTAGTTGTCCATGTCCGCCATCTGGCCGTCACGGAACGGGAAGCCGGTGCCCAGCAGGGCGCCTTCCAGGCTTGTGCGCGAGCTGACGCGCAGGCGGCGGCCATTGAGTTGGGCGCCACGGCCACGGCTG  >  ACB122/968534‑968799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑