| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,138,494 | T→G | 43.0% | intergenic (‑108/+33) | pigA_1 ← / ← hemR | Heme oxygenase PigA/Hemin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,138,494 | 0 | T | G | 43.0% | 1.3 / 4.1 | 25 | intergenic (‑108/+33) | pigA_1/hemR | Heme oxygenase PigA/Hemin receptor |
| Reads supporting (aligned to +/- strand): ref base T (3/11); new base G (8/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
AGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCG > ACB122/1138367‑1138623 | aGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCCCGTAGCGGGCGGGccccc > 2:153713/1‑132 (MQ=255) ggCTGGGCGGACGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCACATCGCGGGCGGGGCCGCaaa > 8:93857/1‑135 (MQ=255) gggcggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTGGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCgg < 7:56961/132‑1 (MQ=255) tGGGGGCGGCCTTGGAAAGAGGCCGGCTAGAAACAAGACGAACCCGCGGCCGCGAGACAGTAAAAAACCCCGGTA‑TTTGCGTTGCCAGACCCGGCCTCTTCGCGGGCGGGCCCGAAACGAGGCCGGTACAGACg < 4:158564/134‑1 (MQ=255) gggAGTCCTTGGAAAGAGGGCGTCTACATACACGACGAAACAGCGGGAGCGAGACAGTAAAAAACGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGGGCCCGCAAAGAGGCCGGTACAGACGTAAc < 7:465614/135‑1 (MQ=255) aGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGGGGGCCCCCAAAGAGGCCGGGTCAGGCCTTACGcc > 7:461182/1‑135 (MQ=255) cccTTGCAACGAGGGCGGCTAGATACAAGACGAAACCGCGGGAGCGAGACAGTAAAAAACGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTc < 5:464921/134‑1 (MQ=255) cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTAGCGCGCGTGCCCCCAAAGAGGCCCGTTCAAACCGAACGcc > 5:239068/1‑131 (MQ=255) gcgggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCa < 8:151436/133‑1 (MQ=255) cGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGGGCCCGCAAAGAGGCCGGTACAGACCTGACCCCGCCGATATCCCCAAACACATTTATAACGCAGt > 3:356488/1‑135 (MQ=255) aGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCGCGTCGCGGGCGGGCCCGCAAAGAGGCCGGGTCCGCCGGTACGCCGCCGATATCCCACACCCCATTTATACCGCAGGTGCGcc > 1:30414/1‑135 (MQ=255) gAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGGGCCCGCCAAGAGGCCCGGTCCGACGGAACGCCGCAGATATCCCAAACCCCATTGATACCGAAGGTGCGCCCCGGc > 3:236621/1‑135 (MQ=255) gAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCGCTTCGCGGGGGGGGCCGCCAAGAGGGCGGGACACACGTAACGCCGCCGCTATCCCCAACCACAATGCTACCGAAGTTGCGcccccg > 8:239418/1‑132 (MQ=255) gAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCGAGTAGCGGGGGGGGCCGCCAAGAGGCCGGGTCAGCCGTTACGCCGCCGCTATACCCAACCCCCTTTATTCCCAACTTGCGcccccg > 4:231879/1‑132 (MQ=255) gaCAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTATCGGGCGTGGCCGCAAAGAGGGCGGTACATACGTAAAGCCTCAGATATCCCAAACCACATTGATACCGCAGTTGCGCCCCCGCAAgg > 8:236899/1‑133 (MQ=255) gaaaaaaacGCCGGTA‑TTGGCGTGGCCAGTCCCGGCGTCTGCGCGGGCGGGCCCGAAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGc < 3:463586/133‑1 (MQ=255) gtaaaaaaCCCGGGATTTTGCGTTGCCAGACCCGGCATCTTCTCGGGCGTGCCCGAAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGc < 8:122667/133‑1 (MQ=255) tA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct < 5:182150/135‑1 (MQ=255) tA‑TTGGCGTTCCCAGTCCCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct < 2:442018/135‑1 (MQ=255) ccAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca < 2:308099/134‑1 (MQ=255) tACCGGCCTGTTCGCGGGCGTGCCGGAAAAGATGGCGGTACAGACGTAAGGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGGATGGTCTGGCGGTCGATGTTGGCTGGCTGGGTCACAgcc < 7:63776/135‑1 (MQ=255) tctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCAcc < 6:239068/135‑1 (MQ=255) tacgcgGGCGTGCCCGCAAAGAGGCGGGTACAGACGTAACGCCTCAGATATGCCAAACCACATTGATACCGATCTTGCGCCCCCGCATGGTCAGGCGGTCGAGGTGGGCTGGCTGGGTCACAGCCGGCTCACCCt < 4:111921/133‑1 (MQ=255) gcgGGCGTGGCCGCCAAGAGGCCGGTCCAGACGGAACGCCTGAGATATCCCACCCCCCCTTGGTACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTGGATGTTGGCTGGGTGGGTCCCCGCCGCCTACCCCGGGc > 8:331925/1‑135 (MQ=255) gggCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcg < 8:151102/135‑1 (MQ=255) | AGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTA‑TTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCG > ACB122/1138367‑1138623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |