Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,157,983 A→C 22.3% S162A (TCG→GCG)  hexR_1 ← HTH‑type transcriptional regulator HexR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,157,9830AC22.3% 80.7 / 2.2 63S162A (TCG→GCG) hexR_1HTH‑type transcriptional regulator HexR
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base C (11/4);  total (38/25)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCTGCGGG  >  ACB122/1157852‑1158113
                                                                                                                                   |                                                                                                                                  
gTTTTCCCGGGCCCGGCGCGCCGCTGCGACCAGTGCGCGGGGGCGCCCGGTGTAGCAAATGATCACGAACAGAGCGCCGGTGGGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACAGCGGCGTGGGCCGacac                                                                                                                                 <  6:51909/135‑1 (MQ=255)
 gtttCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGCGCACGCACAGATCGCCGGTGTGGGCGCCCGAGGCCCGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACCCg                                                                                                                                >  4:197620/2‑135 (MQ=255)
      aCGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCACCGcccc                                                                                                                           >  1:434260/1‑133 (MQ=255)
       cGTCCCCGGCGCGCCACTTCGACCAGTTCGCGGGGGCGCCCGGTGTAGTAAATGAGCACGAACAGATCGCCGTTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAgg                                                                                                                          <  4:159056/135‑1 (MQ=255)
              ggCGCGCCATTGCGACCTGTGCGGGGGGGCGCCCGGTGTAGGAACTGAGCACGAACCGATCGCCGGTGGGGGGGACCGAGGCCAGCAGGGGCTGCATCAGCACAGCGGGGTGGGCCGACACGGCCAGGTTGAAGc                                                                                                                   <  6:57138/135‑1 (MQ=255)
              ggCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGTAAATTATCACGAACAGATCGCGGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGc                                                                                                                   <  5:128214/135‑1 (MQ=255)
              ggCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCCCGGCCAGGGTGAAGc                                                                                                                   >  3:293350/1‑135 (MQ=255)
                       ctgcGACCAGTGCGCGGGTGCGCCCGGTGTTGGAAATGATCACGAACAGATCGCCGGGGGGGGCGACCGAGGCCCGCATGCGCTGAATCAGCACATCGGCGGGGCCCGACACGGCCAGGTTGAAGCGGAAGAACt                                                                                                          <  3:357059/132‑1 (MQ=255)
                           gACCTGTTCGCGGGGGCGCCGGGTGTAGCAAATGACCACTAACCGTGGGCGGGTGTGGGCGACCGAGGACAGCATGCGGGGCATCAGCACATCGGCGTGGGCCTACACGGCCAGGTTGAAGCGGAAGAACTtgtg                                                                                                      <  6:284921/135‑1 (MQ=255)
                           gACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTtgt                                                                                                       <  8:33087/134‑1 (MQ=255)
                            aCCAGTTCGCGGGTGCGCCCGGTGTATGTAATCAGCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCTGCATGCGCTGCATCAGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTtgtg                                                                                                      <  8:417980/134‑1 (MQ=255)
                                    gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCCCCGGCCGGGTGGAGCGGAAGAACTTGTGCTGCGCCTc                                                                                             >  5:53395/1‑135 (MQ=255)
                                    gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTTAAGCGGAAGAACTTGTGCTGCACAt                                                                                              >  4:421419/1‑134 (MQ=255)
                                          gcgcCGGGTGGAGCAAAGCATCCCCACCCGAGCGCCGGTGGGGGCGACCGAGGCCAGCTGGCGCTGCATCCGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGc                                                                                       <  1:114946/135‑1 (MQ=255)
                                          gcgcCCGGGGTAGCAAATTAACACGAACCCAGCGCCGGGGGGGGCGCCCGAGGCCAGCATGCGCTCCATCAGCACAGGGGCGTGGGCCCCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGc                                                                                       <  5:92763/135‑1 (MQ=255)
                                                 gtgaAGGAAATCACCCCGACCCGAGCGCCGGGGTGGGCGACCGCGGCCCGCAGGCGCGGCAGCCGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCggg                                                                                <  4:293350/135‑1 (MQ=255)
                                                     aGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGc                                                                              >  8:241226/1‑133 (MQ=255)
                                                     aGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGc                                                                              >  8:247744/1‑133 (MQ=255)
                                                         aaTGACCACGAACAGATCGCGGGGGGGGGCGACCGAGGCCAGCATGCGCTGCAGCAGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGgc                                                                        <  8:137709/135‑1 (MQ=255)
                                                          aTGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGAAACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGgcg                                                                       >  8:281892/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGGAGCGGAAGAACGTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAg                                                                  >  4:85003/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGAg                                                                  >  8:376546/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCCGGGCCCCCGGGGCTGAGGCGCCGAg                                                                  >  4:71155/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGCGGCGCCGAg                                                                  >  4:199874/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGCGGCGGAGGCGCCGAg                                                                  >  4:291716/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCACGTTGAAGCGGAAGAACTTGTGCTGCCCATCCACGGCCACCGCGGCTGAGGCGCCgcc                                                                  >  3:200636/1‑133 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGAAAAGGCCAGGTTGAAGCGGAAGAACTTGTGCTTAGCATCCAGGGCCACCGGGGCTGAGGCGCCGAg                                                                  >  5:216465/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCCACCCGGCCAGGTTGAAGCGGAAGCACTTGTGCTGCGCATCCAGGGCCCCCGGGGCCGCGGCGCCGAg                                                                  >  2:159198/1‑135 (MQ=255)
                                                                ccGAACAGATCGCCGGTGGGGGCGCCCGAGGCCCGCATGCGCTGCATCAGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAgg                                                                 <  3:232133/134‑1 (MQ=255)
                                                                cagaccAGAGCCCCGGGGGGGCCGACCGAGGCCAGCAGGCGATGCAGCCGCACAGGGGGGGGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAgg                                                                 <  5:95451/130‑1 (MQ=255)
                                                                   aaCAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACCCGGCCAGGGTGAAGCGGAAGCACTTGTGCTGCGCATACAGGGCCCCCCGGGCTGAGGCGCCGAGGcc                                                               >  8:401941/1‑134 (MQ=255)
                                                                           gCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGAGGCCGAAGAAGTg                                                      >  3:400794/1‑135 (MQ=255)
                                                                           gCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGACGTg                                                      >  6:12426/1‑135 (MQ=255)
                                                                           gCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGGTGAAGCGGAAGAACTTGTGGTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGCAGAAGTg                                                      >  5:212234/1‑135 (MQ=255)
                                                                           gCCGGGGGGGGCGCCCGTGGCCAGCCGGCGCGGCATCAGCACCTCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCGGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTg                                                      <  6:140689/135‑1 (MQ=255)
                                                                             cGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCCGCACGGCCAGGGTGTAGCGGAAGAACATGTGCTGCGCATCCAGGGCCACCGGGGCGGAGGCGCCCAGGCCGAAGAAGTgg                                                     >  8:191542/1‑134 (MQ=255)
                                                                               gtgtGGGCGACCGAGGCCAGCATGGGCTGAATCAGCACATCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGTGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                                  <  8:429882/135‑1 (MQ=255)
                                                                               gtgtGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGGCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                                  >  7:21241/1‑135 (MQ=255)
                                                                                 gtgGGCGACCGAGGCCAGCATGCGCTGCATCCGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTg                                                <  4:362226/135‑1 (MQ=255)
                                                                                       gACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTTCTGCGCATCCAGGGCCACCCGGGCTGAGGCGCCGAGGCCGCAGAAGTGGCTCTGCCGGGc                                          >  1:87924/1‑135 (MQ=255)
                                                                                       gACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATACAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATATGCCggg                                           >  7:429882/1‑134 (MQ=255)
                                                                                        aCCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGcc                                         <  4:41951/135‑1 (MQ=255)
                                                                                         ccGCGGCAAGCATGGGCGGCATAAGCACATGGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCt                                        <  8:402415/135‑1 (MQ=255)
                                                                                          cGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATACACGGCCCCCGGGGCTGAGGCGCCCAGGCCGAAGAAGTGGATCTGCCGGGccgg                                       >  4:419065/1‑133 (MQ=255)
                                                                                             ggCCAGAATGCGCTGCATCACCACTGCGGGGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGat                                    <  8:278360/135‑1 (MQ=255)
                                                                                              cagaGCATGCGCTGCAGCAGCCCAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGatc                                   <  6:336934/132‑1 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGGCGACCCGGCCAGGTTGAAGCGGGAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGCGGCGGCGCGGCCGCAGAAGTGGATCTGCCGGGCCTGGATCATCATGt                            >  2:418428/1‑134 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGCCGCCCCGGCCCGGTTGAAGCGGAAGAACTTGTGCTGCGCCTCCAGGGGCACCGGGGCTGAGGCGCCGCGGCCGCAGAAGTGGATCTGCCGGGCCTGGATCCTCATGTc                           >  6:385454/1‑135 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGCCGCCCCGGCCCGGGTGAAGCGGCAGAACTTGTGCTGCGCCTCCCGGGCCCCCCGGGGGGCGGCGCCGAGGCCGCAGAAGTGGGTCTGCCGGGCCGGGCTCGTCATGtg                           >  5:287027/1‑134 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGCCGCCCCGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGCGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGt                            >  6:24980/1‑134 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCCGGGCCCCCGGGGCTGAGGCGCCGAGGCCGCAGAAGTGGATCTGCCGGGCCGGGCTCATCATGTc                           >  8:61093/1‑135 (MQ=255)
                                                                                                        gcTGCATCCGCACCGCGGCGGGGGCCCCCACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCGGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCa                         <  7:261001/135‑1 (MQ=255)
                                                                                                          tGCACCAGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACg                       <  4:164524/135‑1 (MQ=255)
                                                                                                               cAGCACATCGGCGTGGGCCGCCACGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCGGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCGGGATCATCATGTCCCCGgcctg                  >  5:336934/1‑132 (MQ=255)
                                                                                                               cAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGgcgc                   <  8:86308/134‑1 (MQ=255)
                                                                                                               cAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGgcgc                   >  7:86308/1‑134 (MQ=255)
                                                                                                                     aTCGGCGTGGGGCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCAGAGGCGCCCAGGCCGAAGAAGTGGATCTGCCGGGCCAGGATCATCATGTCCACGGCGCGGCTGAc            >  8:311328/1‑135 (MQ=255)
                                                                                                                         ggtggggCCTCCACGGCCAGGTTGAAGCGGAATATCTTGTGCTGCACATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgc        <  3:421419/131‑1 (MQ=255)
                                                                                                                         gCGTGGGCCGACACGGCCAGGTTTAAGCGGAAGAACTTGTGCTGCGCATACAGGGCCAACGGGGCTGAGGCGCCGAGGCCGAAGAAGTGTATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACcttc        >  6:325935/1‑133 (MQ=255)
                                                                                                                         gCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgc        <  7:58249/135‑1 (MQ=255)
                                                                                                                          cGTGGGGCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATACAGGGCCACCGGGGCGGAGGCGCCCCGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATTTCCACGGCGCGGCGGGCcagct       >  4:78590/1‑135 (MQ=255)
                                                                                                                          cGTGGGCCGACCCGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGAGGCCGCAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgct       >  8:37137/1‑135 (MQ=255)
                                                                                                                          cGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCGGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgct       >  7:100440/1‑135 (MQ=255)
                                                                                                                               gCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCCCCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGCTCATCATGTCCACGGCGCGGCTGTCCGGCTGCggg  >  5:85415/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                  
GTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCAGGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCTGCGGG  >  ACB122/1157852‑1158113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑