Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,166,043 G→A 5.5% L21L (CTC→CTT)  xseA ← Exodeoxyribonuclease 7 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,166,0430GA 5.5% 238.7 / 3.5 73L21L (CTC→CTT) xseAExodeoxyribonuclease 7 large subunit
Reads supporting (aligned to +/- strand):  ref base G (23/46);  new base A (2/2);  total (25/48)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCACCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAAT  >  ACB122/1165913‑1166172
                                                                                                                                  |                                                                                                                                 
gcacCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGtt                                                                                                                               >  6:40049/1‑135 (MQ=255)
  acCTGGGCGGCGCTGTCCTTGAGGGTGAAGTAGATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  7:122372/135‑1 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             >  3:406869/1‑135 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  5:331645/135‑1 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  7:315613/135‑1 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  1:64413/135‑1 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             >  7:399215/1‑135 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  8:374661/135‑1 (MQ=255)
  acCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGGTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTgg                                                                                                                             <  4:98405/135‑1 (MQ=255)
  aacTGGGCGCATCTGTCCTTGAGGGTGAAGTACATGTGTCCGTACTCCTGGCGGGAGATGTTGGAGATTTCGCCTTCCACCCACACGCTGAGGAAAACGTCTTCCAGCAGCACGCGGTCGAGGCCGTTGAGTTgg                                                                                                                             >  6:170317/3‑135 (MQ=255)
            cgcTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTaa                                                                                                                   >  7:313473/1‑135 (MQ=255)
            cgcTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTaa                                                                                                                   <  6:448001/135‑1 (MQ=255)
            cgcTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTaa                                                                                                                   >  6:208436/1‑135 (MQ=255)
               tGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGa                                                                                                                >  7:8281/1‑135 (MQ=255)
               tGTCCTGGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGTGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGa                                                                                                                <  4:232763/135‑1 (MQ=255)
                 tCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGAcc                                                                                                              <  8:461163/135‑1 (MQ=255)
                  ccTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGAcc                                                                                                              <  5:40088/134‑1 (MQ=255)
                  ccTTGAGGGTGAACTACTTGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACATGCTGCGGAAAACGTCTACCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGAcc                                                                                                              <  3:317601/134‑1 (MQ=255)
                       aGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTcgc                                                                                                          <  4:34995/133‑1 (MQ=255)
                            gAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAg                                                                                                   >  1:213432/1‑135 (MQ=255)
                              aGTACATGTGGCCGGACTCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGAAGGTAAGGACATCGCGGTCCAGcc                                                                                                 >  6:301824/1‑135 (MQ=255)
                              aGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTcgc                                                                                                          >  7:124939/1‑126 (MQ=255)
                              aGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTcgc                                                                                                          <  8:124939/126‑1 (MQ=255)
                              aGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGcc                                                                                                 >  3:137972/1‑135 (MQ=255)
                              aGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGcc                                                                                                 >  8:212631/1‑135 (MQ=255)
                               gTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGccc                                                                                                <  8:367404/135‑1 (MQ=255)
                               gTACATGTGGCCGGACGCCGGGCGGGAGAGGTTGGAGATTTCGCCTTCCACCCACACTCTGCGGAAAACGTCTTCCAGCAGCACGCGGTCGCGGCCGTTGAGTTGGCTGACTGTAAGGACCTCGCGGTCCAGccc                                                                                                <  4:383659/135‑1 (MQ=255)
                                tACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCg                                                                                               <  4:426066/135‑1 (MQ=255)
                                tACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGATGAGTTGGCTGACGGTAAGGACCGCGCGGTCCCGcc                                                                                                 >  2:122740/1‑133 (MQ=255)
                                         ccGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCg                                                                                      <  2:295435/135‑1 (MQ=255)
                                         ccGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCg                                                                                      <  8:191397/135‑1 (MQ=255)
                                          cGGTCGCCGGGAGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCTCGCGGGCGCGGCCGTTGAGTTTGCTGACTGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGa                                                                                     <  7:436082/135‑1 (MQ=255)
                                          cGGACGCCGGGCGGGCTCGGTTGGAGATTTCGCCGTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGa                                                                                     <  2:425560/135‑1 (MQ=255)
                                          cGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGAcc                                                                                                              <  1:293549/110‑1 (MQ=255)
                                          cGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGAcc                                                                                                              >  2:293549/1‑110 (MQ=255)
                                           ggACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCg                                                                                      >  8:26256/1‑133 (MQ=255)
                                             aCGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAg                                                                                  >  5:115558/1‑135 (MQ=255)
                                               gCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAggg                                                                                <  7:234390/135‑1 (MQ=255)
                                                    gcgggcgAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCttt                                                                           <  7:212631/135‑1 (MQ=255)
                                                      gggcgAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCtgtgg                                                                         >  5:350482/1‑134 (MQ=255)
                                                             gatGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTGGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCAtggc                                                                  <  3:337592/133‑1 (MQ=255)
                                                                                 cccacACGCTGCTGAAAACGTCATCCAGCAGCACGCGGGCGCGGCGGTTGAGTTGGCTGTCGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCg                                              <  6:62484/135‑1 (MQ=255)
                                                                                  ccacACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCgg                                             <  1:392786/135‑1 (MQ=255)
                                                                                  ccacACGCTGCGGAAAACGGCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCgg                                             <  8:227641/135‑1 (MQ=255)
                                                                                    acacGCTGCGGAAAAGGTCTTCCCGCAGCAGGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGcc                                           <  2:221542/135‑1 (MQ=255)
                                                                                        gCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTGCGAAAGGGTCTTTGATAATGGCTGGCATCAGAAATGACATCGGCCCCgg                                       >  4:420397/1‑135 (MQ=255)
                                                                                         cTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGt                                      <  6:264850/135‑1 (MQ=255)
                                                                                            cGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTg                                                                                                                              >  4:61837/1‑44 (MQ=255)
                                                                                            cGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTg                                                                                                                              <  3:61837/44‑1 (MQ=255)
                                                                                             ggAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTtgtc                                  >  8:395341/1‑135 (MQ=255)
                                                                                              gAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGGTCGAAAGGGTCTTTGCTCATTGCTGGCATCATTAATGCCATCGGCCCCCGGtgtct                                 >  6:86649/1‑135 (MQ=255)
                                                                                               aaatCGGCTTCCAGCAGCACGCGGGCGCGGCCGTTGTGTTGGCTGACGGTAAGGAACTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTtgtctg                                <  6:266346/135‑1 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTtgtctg                                <  8:270847/135‑1 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTtgtctg                                <  8:399215/135‑1 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTAAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGa                                                   >  1:83765/1‑116 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTAAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGa                                                   >  1:76592/1‑116 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTAAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGa                                                   <  2:76592/116‑1 (MQ=255)
                                                                                               aaaaCGTCTTCCAGCAGCACGCGGGCGCGGCCGTTAAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGa                                                   <  2:83765/116‑1 (MQ=255)
                                                                                                     tCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCTGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGc                          <  4:406869/135‑1 (MQ=255)
                                                                                                     tCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGc                          <  7:26256/135‑1 (MQ=255)
                                                                                                     tCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGc                          <  6:115558/135‑1 (MQ=255)
                                                                                                     tCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGGCTGTCTTGc                          <  5:467/135‑1 (MQ=255)
                                                                                                       ttCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAATGGTCTTTGATAATGGGTGGCATCATTAATGAAATCGGCCCCGGTTGTCTGTCTTGcc                         >  2:84101/1‑134 (MQ=255)
                                                                                                           agcagcACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAg                    <  8:403716/135‑1 (MQ=255)
                                                                                                           agcagcACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAg                    <  3:295060/135‑1 (MQ=255)
                                                                                                                caCGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCa               <  3:44542/135‑1 (MQ=255)
                                                                                                                 aggcgGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAg              <  8:381208/133‑1 (MQ=255)
                                                                                                                 aCGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAg              <  6:152450/135‑1 (MQ=255)
                                                                                                                    cgGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATTACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCCGCCt           >  6:224523/1‑135 (MQ=255)
                                                                                                                    cgGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCt           <  1:383438/135‑1 (MQ=255)
                                                                                                                      ggCGCGGCCGTTGAGTTGGCTGACGGAAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTg         <  1:122740/135‑1 (MQ=255)
                                                                                                                          cgGCCGTTGAGTGGGCTGACGGTAAGGACCTAGCGGTCCAGCCCGAGTCTTTCGAAAGGTTATTTGATCATGGCTGGCATCATAAATTACATCGGCCCCGGTTTTCTGTCTTGCCCCTAGTGTCAGCCTTGAacc     <  5:170317/135‑1 (MQ=255)
                                                                                                                             ccGTTGAGTTGGCTGACGGTAAGGACCGCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAAt  <  2:168320/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
GCACCTGGGCGCCGCTGTCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAAT  >  ACB122/1165913‑1166172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑