Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,195,943	T→G	11.9%	V236G (GTT→GGT)	CJ019_01125 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,195,943	0	T	G	11.9%	121.5 / 2.6	60	V236G (GTT→GGT)	CJ019_01125	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/38);  new base G (6/3);  total (19/41)
Fisher's exact test for biased strand distribution p-value = 2.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGCGTAC  >  ACB122/1195812‑1196077                                                                                                                                     |                                                                                                                                       tttgCCAACCGTATTTTCGCCGCTCGTCACACTGCCCGGCCTGGTCGACCGCGCCCGCAAGGTGCGGTCGTTTGCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTg                                                                                                                                     <  3:451428/132‑1 (MQ=255) ttGGCCAACCGTATTTGTGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCAGCTGGCAGCTTGCCAGGTGCTGGGT‑ACCGgttt                                                                                                                                     >  4:307200/1‑134 (MQ=255) ttGGCCAACCGTATTTGCGCCGCTCGAAACACCGCCCGGCCTGTTCGACCGCGCCCGCAATGTGCGCTCGTTTGCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTg                                                                                                                                     <  3:443907/135‑1 (MQ=255)       aaCCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTgg                                                                                                                               <  3:213577/135‑1 (MQ=255)                 gcgcCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCCGCCGCGGCTGCGGGCAGCTTTGGGGGGGCTGGCT‑GCCGTTTGGGGTGGGCTGTGGGTg                                                                                                                     >  7:141683/1‑135 (MQ=255)                 gcgcCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTAGTTGCCGGCCGCCTCCCGCCCGCAGCACCGGCATCTGTGAGCGTTCCGGGTGCTGGGTAAAGGTTTGGGGTGGcgggggt                                                                                                                        >  7:270190/1‑127 (MQ=255)                  cgccgcTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTg                                                                                                                     <  7:412562/134‑1 (MQ=255)                    ccgcTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCGGGGGCTGGCT‑ACCGTTTGGGGTGGCCGGTGGTTGAg                                                                                                                   >  3:81789/1‑134 (MQ=255)                       cTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCCGCCGCCGCGGCGGCGGGGCGCTTTCCTGGTGCGGGGG‑ACCGGTTGGGGGGGGCGGTGGGTGAgggcg                                                                                                               >  1:327774/1‑131 (MQ=255)                          gACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCGGCTGGCAGCTTGCCTGGGGCTGGCG‑ACCGTTTGGGGTGGCCTGTGGGTGCGTCAGc                                                                                                              >  8:425287/1‑133 (MQ=255)                              cacTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGc                                                                                                        <  5:347356/135‑1 (MQ=255)                                 tGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTc                                                                                                     <  2:354149/135‑1 (MQ=255)                                    ccggccggTGCGACCGCGCCCGCATGGTGCGCGCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAgg                                                                                                  <  5:278221/135‑1 (MQ=255)                                    ccGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCCGCCGCCGCGGCGGCGGGCCGCTTGCCGGGTGCGGGCG‑ACCGGGTGGGGGGGCCGGTGGTTGAGTCCGCCATGGCGGCAgg                                                                                                  >  1:384672/1‑135 (MQ=255)                                    ccGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCCGCAGCGGCGGCTGGCCGCTTGCCTGGGGCTGGCT‑ACCGTTTGGGGGGGGCTGGGGTTGTGGCAGCAATGGCGTCCgg                                                                                                  >  7:25825/1‑135 (MQ=255)                                    ccGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCCGCTTTGCTGGTGGTGGGG‑ACCGTTTGGGGTGGGCGGTGGGTGCGTGAGCAATGGGGTCAgg                                                                                                  >  4:446022/1‑135 (MQ=255)                                    ccGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAg                                                                                                   <  1:135084/134‑1 (MQ=255)                                    ccGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAg                                                                                                   <  1:400820/134‑1 (MQ=255)                                     cGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCCGCCCCAGCGGCGGCGGGCCGCTTGGCGGGGGCGGGGG‑TCCGGTTGGGGGGGGCGGGGGGGGGGTCGGGCATTGCGTCAGgc                                                                                                 >  3:58107/1‑135 (MQ=255)                                      ggCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCCGCAGCCGCGGCTGCTGGCAGCGTGGCTGGGGGTGGCG‑TCCGGGTGGGGTGGGCTGTGGTGGTGTCAGGAATGGGGGCAGgcg                                                                                                >  8:103673/1‑135 (MQ=255)                                               gACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTg                                                                                        <  6:217530/134‑1 (MQ=255)                                               gACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCGGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTg                                                                                        <  7:358955/134‑1 (MQ=255)                                                    cgcCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAg                                                                                  <  7:265910/135‑1 (MQ=255)                                                         gCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAgg                                                                             <  5:64951/135‑1 (MQ=255)                                                         gCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAgg                                                                             <  2:384672/135‑1 (MQ=255)                                                               tGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGGTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCGTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCAtt                                                                        <  2:176162/134‑1 (MQ=255)                                                                           cggccgCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCggg                                                            <  6:339476/134‑1 (MQ=255)                                                                            ggccgCCTCCCGCCAGCAGCAGCGGCTGCTGGGAGCTTGGCTGGTGCGGGGG‑TCCGGTTGGGGTGGGCGGGGGGTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCCGCCAGGTGCTTGCCGTGCCCGGGGa                                                          >  6:441506/1‑135 (MQ=255)                                                                               cgccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCa                                                       <  7:301354/135‑1 (MQ=255)                                                                               cgccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCa                                                       <  4:220802/135‑1 (MQ=255)                                                                                     ccGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTATGGCCCAGCCCGGTCCTTGCCGTGACCGGGGAGCCGGCCAg                                                 >  8:428627/1‑135 (MQ=255)                                                                                      cGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGc                                                <  3:180787/135‑1 (MQ=255)                                                                                           gcagcagcGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGGTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCg                                           <  6:49349/135‑1 (MQ=255)                                                                                             agccgcGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGc                                         <  8:25652/135‑1 (MQ=255)                                                                                             agcagcagcTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTGGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGc                                         <  2:36805/135‑1 (MQ=255)                                                                                             agcagcGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGc                                         <  1:313650/135‑1 (MQ=255)                                                                                                   ggCTGCTGGCAGCTTGGCTGGTGCGGGCT‑ACCGTTTGGGGCGGCCTGTGGGTGAGTCAGCACTGGCGTCAGGCGCAACTCTGGCGCAGACAGGTCATTGCCGGGACCGGGGATCAGGCCAGCCCTCGGCACGCgg                                   <  1:336988/135‑1 (MQ=255)                                                                                                   ggCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCgg                                   <  8:399733/135‑1 (MQ=255)                                                                                                   ggCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCgg                                   <  3:412517/135‑1 (MQ=255)                                                                                                   ggCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGGTGAGGCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCgg                                   <  7:160873/135‑1 (MQ=255)                                                                                                   ggCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGGGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCgg                                   <  3:419681/135‑1 (MQ=255)                                                                                                    gctgctGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGc                                  >  8:315368/1‑135 (MQ=255)                                                                                                    gctgctGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGc                                  <  7:425287/135‑1 (MQ=255)                                                                                                    gctgctGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGGGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGc                                  <  4:125391/135‑1 (MQ=255)                                                                                                         tgGCAGCTTGCCTGGTGCTGGCT‑ACCGGTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGcaca                               <  4:341902/133‑1 (MQ=255)                                                                                                            cAGCTTGCCTGGTGCTGGCT‑CCCTGTTGGGGTGGCCGGTGGTTGCGTCAGAAATGGCGTCCGGCGAAACTCTGGAGCCGCCAGGTCCTTGCCGGGCCCGGGGAGCATGCCAGCCCTCGGCACGCGGCCAAGGccc                          >  6:427185/1‑135 (MQ=255)                                                                                                            cAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGc                                  >  8:372596/1‑127 (MQ=255)                                                                                                            cAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGc                                  <  7:372596/127‑1 (MQ=255)                                                                                                            cAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCGGTGGGTGAGTCAGCAATGGCGGCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGccc                          <  2:422610/135‑1 (MQ=255)                                                                                                             aGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGCCCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGccc                          >  3:217002/1‑134 (MQ=255)                                                                                                                  gCCTGGTGCTGGAT‑ACCTTATGGGGTGGCCTGTGGGTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCATGCCAGCCCTCGGCACGAGGCCCAGGCCCTCaatc                    >  4:217577/1‑133 (MQ=255)                                                                                                                   ccTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAg                     <  3:305287/133‑1 (MQ=255)                                                                                                                        tGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCt                <  3:56142/133‑1 (MQ=255)                                                                                                                         gCTGGCT‑ACCGTTTGGGGGGGCCTGTGGTTGAGTCAGCAAGGGCGGCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTgcg             <  3:275151/135‑1 (MQ=255)                                                                                                                          cTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTgcg             >  5:429329/1‑134 (MQ=255)                                                                                                                           tGGCT‑ACCGGTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGcgcg           <  4:163280/135‑1 (MQ=255)                                                                                                                              cT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCgaga        <  3:161812/135‑1 (MQ=255)                                                                                                                              cT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCgaga        <  6:385662/135‑1 (MQ=255)                                                                                                                              cT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCgaga        <  6:268490/135‑1 (MQ=255)                                                                                                                                 aCCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGc      >  7:121891/1‑135 (MQ=255)                                                                                                                                   cGTTTGGGGTGGCCTGTGGGTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGCGt    <  4:362295/135‑1 (MQ=255)                                                                                                                                     tttGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGCGTAc  <  1:331132/135‑1 (MQ=255)                                                                                                                                     tttGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGGCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGCGTAc  <  2:327774/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCT‑ACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCCCTCAAGCGCCTGCGCGAGAGCGTAC  >  ACB122/1195812‑1196077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑