Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,339,139	T→G	20.9%	*269C (TGA→TGC)	udh ←	Uronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,339,139	0	T	G	20.9%	97.4 / 3.2	69	*269C (TGA→TGC)	udh	Uronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base G (10/7);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGC  >  ACB122/1339010‑1339266                                                                                                                                  |                                                                                                                                aaTTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGtt                                                                                                                            <  3:114933/135‑1 (MQ=255) aaTTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTAGCAGTTCATGGCCGTGGCTCCTGGCGTGGGGGGAGtt                                                                                                                            >  5:35407/1‑135 (MQ=255) aaTTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTAACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCTGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGt                                                                                                                             >  6:422072/1‑134 (MQ=255)              gAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGggccggcc                                                                                                               >  7:240509/1‑135 (MQ=255)              gAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGCAGTTGGACGggccggcc                                                                                                               >  7:438883/1‑135 (MQ=255)                          aGTACAGGGCGTGTTCCCCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTGCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                   <  1:79048/135‑1 (MQ=255)                          aGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                   <  8:360592/135‑1 (MQ=255)                          aGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                   <  7:90783/135‑1 (MQ=255)                          aGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                   <  4:349764/135‑1 (MQ=255)                          aGTACAGGGCCTGTGCACCGGGCTGCCACACATGGTTTTCGCCGGTACCGGTGCGGGCGTCGCCGTTCAGGTCTCAGTTCATGGCCGTGCCGCGTGGCGGGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAgcg                                                                                                   <  8:218426/135‑1 (MQ=255)                            tACCGGGCCGGGGCACCGGGGGGCCACACCGGGCGTGCGCCGGTACCGGTGCGGGCGTCGACGATCAGTTCGCAGTGCATGGCCGGGCCGCCTGGCGTGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAGcgcc                                                                                                 <  5:298054/135‑1 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGCCGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAACGCgccgcc                                                                                              >  2:40382/1‑135 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTTGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGcgccgc                                                                                               >  8:106023/1‑134 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCgccgcc                                                                                              >  2:33890/1‑135 (MQ=255)                               aGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTAGCAGTTCATGGCCGTGCCTCCTGGGGTGGGGGGCTGTGAAAGGGCCGGCCCCGGGGACAgccgcccc                                                                                              >  6:283891/1‑129 (MQ=255)                                gggCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTGGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGccc                                                                                             <  7:106023/135‑1 (MQ=255)                                    cTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTg                                                                                           >  4:177662/1‑133 (MQ=255)                                    cTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCGGGCGGGGGGTGAGTTGAACGGGCCGGGCCCGGCGCAACCGcccccccg                                                                                           >  2:438091/1‑131 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGCCGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGCAGTTGGACGGGCCGGCCCCGGCGGAAGCGCCGCCCTGGTGGa                                                                                      >  5:310639/1‑133 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGGCGCCCGGCGGGGGGGGCGTTGGACCGGGCGGCCCCGGCGAAACCGCCCCCCCGGGTGAcc                                                                                    >  5:16611/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGGGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCCCCGCCCTGGTAGAcc                                                                                    >  7:108375/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGAcc                                                                                    >  5:241812/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGAcc                                                                                    >  5:220841/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGAcc                                                                                    >  7:48942/1‑135 (MQ=255)                                         cACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGAcc                                                                                    >  3:193855/1‑135 (MQ=255)                                               ggTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGGGGGGTCAGTTGCACGGGCCGGCCCCGGCGAAACCGCCGCCCGGGTAGACCATgggc                                                                              >  3:253951/1‑132 (MQ=255)                                                  gCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCGGGCGGGGGGGCAGGTGAACCGGCCCGCCCCGGCGCAAGCGCCGCCCGGGTAGACCATGCTCggg                                                                           >  1:113918/1‑135 (MQ=255)                                                    cacccaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACCATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACCGCGAAAGCGCCGCCCTGCTAGACCAGGCTCTGGTc                                                                         >  2:23826/1‑135 (MQ=255)                                                    cacacaGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCGCGGGTc                                                                         >  7:37305/1‑135 (MQ=255)                                                          gggCTTTCGCCGGTACCGTTGCGGGCGGCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATcggc                                                                   <  2:5166/135‑1 (MQ=255)                                                           ggCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTTGGGGGAGTTGAACGGGCCGGCCACGGCGAAATCGCCGCCCTGGTAGACCATGCTCGGGGGATcggcg                                                                  >  2:399668/1‑135 (MQ=255)                                                              tttCGCCGGTACCGTTGCGGGCGTCGCCGCTCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGGGAGTTGCACGGGCCGGCCCCCGCGAAATCGCCCCCCAGGTAGACCCTGCGCCGGGCATcgggcgcg                                                               >  1:156511/1‑130 (MQ=255)                                                              tttCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGTCCCAGCTCGGGGGATcggcgggg                                                               >  6:99487/1‑133 (MQ=255)                                                                   ccGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGc                                                          >  2:71290/1‑135 (MQ=255)                                                                   ccGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGc                                                          >  1:301063/1‑135 (MQ=255)                                                                   ccGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGGGGGGTGAGTTGCAAGGGCCGGCCCCCGCGCAAGCGCCCCCCGGGTAGACCCTGCGCGGGTCAGCGGCGGCgggggg                                                          >  1:411457/1‑132 (MQ=255)                                                                    cGGTACCGTGGCGGGCGTCGACGAGCAGTTCGCAGTTCCTGGCCGTGCCTCCTGGCGGGGGGGCAGGGGAACGGGCCGGCCACGGCTAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGcc                                                         <  2:445752/135‑1 (MQ=255)                                                                       tACGGTTGCGGGCGTCGAGGATCAGTTCGCTGTTCATGGCCGTGCCTCGTGGGGGGGGGTCTGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                      <  5:99487/135‑1 (MQ=255)                                                                       tACCGTTGCGGGCGTCGACGATCAGTTCGCTGTTCTTGGCCGGGCCTCCTGGCGGGGGGGCTGTTGACGGGGCGGGCCACGGCGAACGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                      <  6:16611/135‑1 (MQ=255)                                                                       tACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCGGGGCCTCCTGGCGGGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                      <  4:9929/135‑1 (MQ=255)                                                                       tACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGGGCCGCCTGGCGTGGGGTCAGTTGACCGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                      <  1:438091/135‑1 (MQ=255)                                                                       tACCGTTGCGGGCGGCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGt                                                      <  4:30720/135‑1 (MQ=255)                                                                          cGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCCCGGCGCAAGCGCCGCCCGGGGAGCCCATGCTCGGGGCATCGGCGGCGGGTGCCGGGTgg                                                   >  3:413812/1‑135 (MQ=255)                                                                                       gacgaTCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGACCCGGCCGGCCCCGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACCGCTg                                      >  6:233804/1‑135 (MQ=255)                                                                                        acgaTCAGTTCGCAGTTCATGGCCGGGCCGCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTgc                                     <  1:118129/135‑1 (MQ=255)                                                                                          gaTCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTgcgc                                   <  8:232384/135‑1 (MQ=255)                                                                                          gaTCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTgcgc                                   <  4:346507/135‑1 (MQ=255)                                                                                           aTCAGTTCGCAGTTCATGGCCGGGCCTCCGGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGcgcg                                  <  1:2651/135‑1 (MQ=255)                                                                                               gTTCGCAGTTCATGGCCGTGCCTGCTGGCGTGGGGGGAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGGAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGGTGGGGCTCCCCCGCTGCGCGGAAg                              >  6:25777/1‑135 (MQ=255)                                                                                                     aGTTCTTGGCCGTGCGTCCTGGCGTGGGGTCAGTGGACCGGGCGGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  7:18913/135‑1 (MQ=255)                                                                                                     aGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  3:205842/135‑1 (MQ=255)                                                                                                     aGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  8:307331/135‑1 (MQ=255)                                                                                                     aGTTCATGGCCGTGCCTCCTGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCAGCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  3:405065/135‑1 (MQ=255)                                                                                                     aGTGCATGGCCGGGCCTCCTGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  4:413812/135‑1 (MQ=255)                                                                                                     aGTGCAGGGCCGTGCCTGCTGGCGTGGGGTCAGTGGCACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCg                        <  2:348940/135‑1 (MQ=255)                                                                                                       ttCATGGCCGTGCCACCAGGCGTGGGGGAAGTTGAACGGGCCGGCCACCGCGAAAGCGCCGCCCTGGTAGCACATGATCGGGGCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCGGCGCGGAACGTTTCGGa                      >  6:78092/1‑135 (MQ=255)                                                                                                        tCATGGCCGGGCCTCCGGGGGGGGGGGCAGTTGAAGGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCGCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAg                     <  6:35407/135‑1 (MQ=255)                                                                                                          aTGGCCGTGCCTCCTGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCt                   <  1:320664/135‑1 (MQ=255)                                                                                                              ccGTGCGTCCTGTCGTGGGGTCTGTTGAAGGGGCCGGCCACGGCGAAAGCGCCGCCATGGTAGACCATGCTCGGGTCATCGTAGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGTTTTCGGAGCTGTc                <  5:422072/134‑1 (MQ=255)                                                                                                              ccGGGCCGCCTGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGGGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTcc               <  4:14602/135‑1 (MQ=255)                                                                                                                  gCCTCCTGGCGGGGGGGCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTgg           <  1:319944/135‑1 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGCCCATGCTCGGGTCATCGGCGGCGGGTGCCGGGTGGGCCTCCACGGCTGCGCGGAAGGGTTCGGAGCTGTGCGTGGGt         >  5:45379/1‑135 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt         >  6:458944/1‑135 (MQ=255)                                                                                                                    ctcctGGCGTGGGGTCAGTTGAAAGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt         >  7:25971/1‑135 (MQ=255)                                                                                                                    ctcctGGCGGGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt         <  3:15540/135‑1 (MQ=255)                                                                                                                    ctccgGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCAGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCCCGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGt         >  1:260861/1‑135 (MQ=255)                                                                                                                        tGGCGTGTGGTCTGTTGATCGGGCCGGGCACGGCGAATGCGACGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACa      <  6:90760/134‑1 (MQ=255)                                                                                                                           cGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATa    <  2:301063/133‑1 (MQ=255)                                                                                                                           cGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTAGCGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGc  >  8:424480/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                AATTGGCGAGCGGGAATGTCTACCCAGTACAGGGCCTGTTCACCGGGGTGCCACACAGGGCTTTCGCCGGTACCGTTGCGGGCGTCGACGATCAGTTCGCAGTTCATGGCCGTGCCTCCTGGCGTGGGGTCAGTTGAACGGGCCGGCCACGGCGAAAGCGCCGCCCTGGTAGACCATGCTCGGGTCATCGGCGGCGGGTGCCGGTTGGGCCTCCACGGCTGCGCGGAAGGTTTCGGAGCTGTCCTTGGGTACATAGC  >  ACB122/1339010‑1339266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑