| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,388,322 | T→G | 46.5% | L336V (TTG→GTG) | ruvB_1 → | Holliday junction ATP‑dependent DNA helicaseRuvB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,388,322 | 0 | T | G | 46.5% | 9.4 / 5.8 | 31 | L336V (TTG→GTG) | ruvB_1 | Holliday junction ATP‑dependent DNA helicaseRuvB |
| Reads supporting (aligned to +/- strand): ref base T (14/2); new base G (13/2); total (27/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCCACA > ACB122/1388199‑1388454 | cATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCCTGCCTACCG‑GCACTTTGGGCGGAATATTGCCGGGCGCGTGGGGGCgggg > 3:185782/1‑135 (MQ=255) cATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCCCCCGGCCTGCCGACCT‑GCCCTTTGGGCGGCAAATTACCGGGCGCTGGGGGGAGGGGGGCGa > 1:919/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCCCGCGGCCGCGTGGGCCCCCGGCCTGCCTACCT‑GCCCTTTGGGCTGAATATTCCCGGGCGCGTGGGGGCGGGGGGCGAgtttt > 7:99161/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCCCTTTGGGCTGAATATTGCCGGGCGCGTGGGGGCGGGGGGCGAttttt > 4:410258/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCATGGCTACCT‑GCCCTTTTGGCTGAATATTCCCGGGCGCGTGGGGGAGGGGGGCGAttttt > 7:64068/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCATGCCTACCT‑GCACTTTGGGCTGAATATTACCGGGCGCGTGGGGGGGGGGGGCGAttttt > 7:9475/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGGGGGCACCCCCCAAGCCTACCTGGCCGATTGGGC‑GTATATTACCGGGCGCGTGGGGGGGGGGGGCGCctttt > 5:43007/1‑135 (MQ=255) aGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGAtttttg > 7:460137/1‑134 (MQ=255) ttGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGGGGTCCCCCCGCCTGCCAACCT‑GCACTTTTGGCGGGATATTGCCGGGGGGGGGGGGGGGGGGGGGGCTTTTTTCggcgccc > 1:218601/1‑129 (MQ=255) cTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCCCCCGGCCTGCCTACCT‑GCCCTTTGGGCTGAATATTCCCGGGCGCGTGGGGGAGGGGGGCGCGTTTTAAGAGCCCGGCGatgaagt > 3:317736/1‑132 (MQ=255) cagcagGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCCTGCCGACCT‑GCCCTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGCTTTTTCCGCGCCAGGCGCTGGATGGCAGCTc > 8:234851/1‑135 (MQ=255) agGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGCGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGaaa > 1:54410/1‑135 (MQ=255) agGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCCTGCCAACCT‑GCACTTTGGGCTGAATATTACCGGGCCCTTGGGGGTGGGGGGCGATTTTTACGCGCCAGGCGAATAATGAAATAACGaaa > 4:261352/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCCCCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATACCCGGGCGCGTGGGGGTGGGGGGCGCTTTTTGCGCGCCCGGCGCTGAATGCCAGATCGAAAGCGACGTTTTTTGGt > 2:44543/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTTGGGGAGGGGGGCGATTTTTGCGAGCCCGGCGATGAATTACAGATCGAAAGCGACTTTTTGTGGt > 3:325988/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTACCGGGCGCGTTGGGGGGGGGGGCGATTTTTGCGCGCCAGGCGAAGTATGAAAGATCGAAAGCGGCATTTTGTGGt > 2:363271/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCA‑GCACTTTGGGCGGAATATTCACGGGGGCGTGGGGGAGGGGGGCGAATTTTACCAGCCCGGCGAAGAAAGAAAGATAGAAAGCCACTTTTtttgtt > 6:175764/1‑133 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCGACCT‑GCACTTTGGGGTGAATATTCCCGGGCGCGTGGGGGAGGGGGGCGCTTTTTCCGAGCCCGGCGATGCATGACAGATCGCAAGCGCCCTTTTGTGGt > 4:461280/1‑135 (MQ=255) cccccgcgGCCCGGGGGCCACCCGGCACGCCAACCG‑GCCAGTTGGCGAGAACATGCCGGGCGGCGTGGGGCAGGGCGCCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTa < 2:367038/131‑1 (MQ=255) cacgcGGCCGCGTGGTCCCCCGGCATGCCTACCT‑GCCCTTTGGGCTGAATATTCCCGGGCGCTGGGGGGGGGGGGGCGTGTTTTGCGCGGCCGGGGCGGTATTGCCGCTCGAAAGCGACGTTTTGTGGTccgtcc > 6:273832/1‑131 (MQ=255) cgGCCCCGGGGCCACCCGGCACCCCTCCCG‑CCACTTCGGGCGCAAGACCCCCGGCCGCGGGGGGGCGGCGCGCGAGTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTg < 8:9475/135‑1 (MQ=255) gCCGCGTGTTCACGCGGCATGCCTACCG‑CCAAGTGCGGCGTAATCTCCCCGGGGGGTTGGGGGAGGGGTGGGATGTTTCGGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTg < 4:80010/133‑1 (MQ=255) gTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCGTGGGGGGGGGGGGCGATTTTTGCGCGCCAGGGGATGAATGACCGATCGAAAGCGCCGTTTTGTGGTCCTACCCCTGGCCTGcc > 3:117704/1‑135 (MQ=255) cGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGCCTTTTTGTGGTCCTACCGCTGGCCTGCCCCTGGTggg > 2:248356/1‑133 (MQ=255) aTGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTg > 2:138173/1‑134 (MQ=255) cACTTTGGGCTGAATATTCCCGGGCGCTT‑GGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGAC‑TTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTa > 1:295372/1‑135 (MQ=255) aCTTGGGGCTGACTCTTCCCGGGCGGTGGGGGGCGGGCGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACa < 7:225630/133‑1 (MQ=255) tGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTGTGTGGTACTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAAt > 7:383627/1‑134 (MQ=255) cTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATgaag > 8:204118/1‑134 (MQ=255) gcTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCcaca > 7:458555/1‑135 (MQ=255) gcTTGGGGGAGGGGGGCGATTTTTACGAGCCAGGCGATGAATGACAGATCGAAAGCGAATTTTTGTGGTCCTACCGCTGGCATGCCCCTGGGGGGCTGGCAATAAGCCATTAATTAAGAAAAAACAGTTGCcaga > 7:397004/1‑133 (MQ=255) | CATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCT‑GCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCCACA > ACB122/1388199‑1388454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |