| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,468,051 | A→C | 35.4% | intergenic (‑777/+24) | algD ← / ← yaaA | GDP‑mannose 6‑dehydrogenase/Peroxide stress resistance protein YaaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,468,051 | 0 | A | C | 35.4% | 3.7 / 2.0 | 27 | intergenic (‑777/+24) | algD/yaaA | GDP‑mannose 6‑dehydrogenase/Peroxide stress resistance protein YaaA |
| Reads supporting (aligned to +/- strand): ref base A (10/6); new base C (2/8); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
TATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTT > ACB122/1467923‑1468164 | tATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTAGCGGCGTCAGCcgc > 7:4568/1‑134 (MQ=255) ggCGGTAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGt > 4:414886/1‑135 (MQ=255) ggCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTAGCGGCTTCAGCCGCGGAGAGGGCGt > 3:48698/1‑135 (MQ=255) ggCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTAGCGGCGTCAGCCGCGAAGAGGGCg > 3:364366/1‑134 (MQ=255) ggCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCATAGCCGCTTCAGCCGCGCAGAGGGCg > 3:236384/1‑134 (MQ=255) ggCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCACTTAGCGGCGTGAGCCGCCCAGCGGGCgg > 3:360479/1‑134 (MQ=255) ggCGATAAAGGGAGGTGCGACGGATTTTTGGAGTCGGATTCGTGGCGTGATGTCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCAAATCGCGGGTTAACCCGCGAAGAGGGCg > 7:73260/1‑134 (MQ=255) aaGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGAGGATCACGGC‑GGCTGACCTCTTCGCCGGGTAAGCCGCGGAGAGGGCGGGACAGGc > 7:109975/1‑135 (MQ=255) cggggcgcCGGTTTTTGGGCGGCGGATGCGTGGCGTGATGGGGGTTTGCCGCGTGCCCTGTGGCCGGGCAGAGGCGGATCCCGGC‑GGCTCACCTCTTCGCGGCTGCCGCCGCGAAGAGGGCGTGACAGGCTCACt < 4:364366/131‑1 (MQ=255) gTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTAGCGGCGTACGCCGCGGAGAGGGCGGGGCCAGCAAAAACCGCGGGGTGGTCgcgccg > 8:313188/1‑132 (MQ=255) gATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCGTCGCGGCGGCCCCCGCGCAGCGGGGGTGGGACGCGCACGCCGCGGGGGGGTGGCGCCGGGacc > 1:380573/1‑134 (MQ=255) tGGCGTGTTGGCGTTTTACAGCGGGACCTGTTCCAGGCCAGCGGGTTAAACCGGG‑GTATTAACTATTCGCGCCTCCCCCCGCTAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAAt < 8:73260/135‑1 (MQ=255) gggggTGAGGGGGGTTGGCCGCGGGCCCTGTGGCCGGGCTGTGGCGTAGCCGGGC‑GGCTGACGTCTGCGCGGCTACAGCGGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAAt < 3:414886/131‑1 (MQ=255) gggCGTGAGGGGGGTTCGCAGCGGGCCCTGTTGCAGGGCAGAGGCGCATCACGGC‑GGCTGACCTGTGCGGGGCTTCCGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAAt < 8:325449/134‑1 (MQ=255) gggCGGTTTGCAGCGGGCCCTGTTGCAGGGCAGAGGCGGAGCCCGGC‑GGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGc < 4:48698/134‑1 (MQ=255) ttGCCGCGGGCCCGGTGGCTGGGCTGAGGCGGAGCCCGGC‑GGCTGACCTCTGCGCGGCTCCCGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACt < 7:247580/135‑1 (MQ=255) tggCAGCGTGCCCTGTTGCAGGGCAGCGGCGGATC‑CGGCGGGCTGACCCCTTCGCGGCTCCCGCGGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACt < 4:461170/133‑1 (MQ=255) gggggCCCTGTGGCTGGGAAGTGGCGGATCACGGC‑GGCGGACCTCTTCGCGGCGGCCGCGGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCg < 8:4568/131‑1 (MQ=255) gCGGGCCCGGTGGCCGGCCAGAGGCGCCGCACGGC‑GGCGCACCGCTGCGCGGCTTCCGCCGCCAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCg < 5:209554/135‑1 (MQ=255) gCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTCGCCGCGTCAGCCGCCAAGAGGGCGTGCCAGGCGCCCGCCGCGGGGTGATCGCGCAGGAACCCCAAATGGGCTGGCTTGGCCTGCTCGGCAc > 6:218888/1‑135 (MQ=255) gCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTAGCGGCGTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGGACACCAAATGGTGTGGCTTGGGCTGCTCGGCAc > 4:118247/1‑135 (MQ=255) gggagagGGGGTTCAGGGC‑GGCTTACTTGTTCGGGGGTACTGCGGGGATGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAcc < 4:114221/132‑1 (MQ=255) tgATCCCGGC‑GGCTCACCTCTTCGCGGCTTCACCCGCCAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCAt < 7:289388/134‑1 (MQ=255) gaccccggc‑ggCTCACCTCTGCGCGGCTTCACCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTAAGCATc < 4:364123/130‑1 (MQ=255) ggcTTACCTATGCGGGGCTGCCGCGGGTAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCtt < 3:446734/135‑1 (MQ=255) ggcTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCtt < 4:139490/135‑1 (MQ=255) ggcTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCtt < 7:156475/135‑1 (MQ=255) | TATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGC‑GGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTT > ACB122/1467923‑1468164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |