BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,567,478	A→G	6.1%	V126V (GTA→GTG)	CJ019_01477 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,567,478	0	A	G	6.1%	319.8 / 5.8	97	V126V (GTA→GTG)	CJ019_01477	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (41/50); new base G (3/3); total (44/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GCGGCGGCCAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCTTCAG  >  ACB122/1567347‑1567611

gcggcgGCCAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGCGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGGagag                                                                                                                                    >  4:70527/1‑135 (MQ=255)

  ggcggcCAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  <  3:183915/135‑1 (MQ=255)

  ggcggcCAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGACGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  <  3:103458/135‑1 (MQ=255)

       ccAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGcc                                                                                                                             <  5:8601/135‑1 (MQ=255)

             aTGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTaaa                                                                                                                       <  4:34647/135‑1 (MQ=255)

             aTGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTaaa                                                                                                                       <  1:452808/135‑1 (MQ=255)

             aTGGCGGGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTaaa                                                                                                                       <  2:462047/135‑1 (MQ=255)

                 cGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGt                                                                                                                   <  5:202810/135‑1 (MQ=255)

                  gTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTg                                                                                                                  <  6:204440/135‑1 (MQ=255)

                  gTGACACGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTg                                                                                                                  <  1:56833/135‑1 (MQ=255)

                    gACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGa                                                                                                                 >  5:350271/1‑134 (MQ=255)

                      cccGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCa                                                                                                              >  6:360680/1‑135 (MQ=255)

                      cccGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCa                                                                                                              >  6:366651/1‑135 (MQ=255)

                      cccGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCa                                                                                                              >  4:334788/1‑135 (MQ=255)

                        cgcgACGAGATCAAGGAAGTGCTGATGCAGCGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCGGGCTGAGTCGGTGTGGGATGAACTTGGCGGAGAGTCTCGCCAGTAAACCGTGATCACg                                                                                                            >  7:79695/1‑135 (MQ=255)

                        cgcgACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACg                                                                                                            >  8:318697/1‑135 (MQ=255)

                        cgcgACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACg                                                                                                            >  2:158372/1‑135 (MQ=255)

                        cgcgACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACg                                                                                                            >  7:72351/1‑135 (MQ=255)

                         gcgACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACgg                                                                                                           >  5:290902/1‑135 (MQ=255)

                          cgacgaGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCTACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACggg                                                                                                          <  5:157106/135‑1 (MQ=255)

                          cgacgaGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACggg                                                                                                          <  6:186886/135‑1 (MQ=255)

                          cgacgaGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACggg                                                                                                          <  5:413070/135‑1 (MQ=255)

                            acgaGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        <  6:350271/135‑1 (MQ=255)

                            acgaGATCAAGGAAGTGCTGAAGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        <  2:227005/135‑1 (MQ=255)

                              gagaTCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAgg                                                                                                      <  8:159107/135‑1 (MQ=255)

                              gagaTCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATGCCGGCGTCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAgg                                                                                                      <  6:117852/135‑1 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTagag                                                                                                                                    >  1:108453/1‑89 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTagag                                                                                                                                    <  2:108453/89‑1 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTagag                                                                                                                                    <  2:114998/89‑1 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTagag                                                                                                                                    >  1:114998/1‑89 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        >  8:62193/1‑117 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        <  7:62193/117‑1 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        >  6:143893/1‑117 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCa                                                                                                        <  5:143893/117‑1 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGgcg                                                                                      >  1:197060/1‑135 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGgcg                                                                                      >  7:233184/1‑135 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGgcg                                                                                      >  2:198627/1‑135 (MQ=255)

                                              tGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGgcg                                                                                      >  8:258904/1‑135 (MQ=255)

                                                     gagCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGccc                                                                               >  1:326424/1‑135 (MQ=255)

                                                      agCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGGCcg                                                                              <  1:1810/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  5:366651/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  5:360680/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  3:285144/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  3:357047/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  6:290902/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  1:251932/135‑1 (MQ=255)

                                                        cgcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGGGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  7:31299/135‑1 (MQ=255)

                                                         gcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            >  3:6324/1‑134 (MQ=255)

                                                         gcgATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGa                                                                           >  5:227208/1‑135 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  >  4:149611/1‑78 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  <  1:452281/78‑1 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  <  3:149611/78‑1 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGtc                                                                                                                                  >  2:452281/1‑78 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCa                                                                                              >  7:210780/1‑114 (MQ=255)

                                                           gATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCa                                                                                              <  8:210780/114‑1 (MQ=255)

                                                             tCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATgg                                                                       >  3:458718/1‑135 (MQ=255)

                                                             tCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATgg                                                                       >  1:114331/1‑135 (MQ=255)

                                                                     ggCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTaca                                                               <  2:326424/135‑1 (MQ=255)

                                                                          tcaggcggcCAATGCCACGTTCCTCCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCAAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGTGCGCTTTCCCGCGAATGGCCTTTAGACAGAt                                                          >  8:328744/4‑135 (MQ=255)

                                                                          tCCGGCGGCCAATGCCACGTTCCCCCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGAt                                                          >  3:289280/1‑135 (MQ=255)

                                                                          tCCGGCGGCCAATGCCACGTTCCCCCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCGGCAACAGGGCGCTTGCCCGCGAAGGGCCTTTACCCCGAt                                                          >  8:413358/1‑135 (MQ=255)

                                                                          tCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGAt                                                          >  1:80762/1‑135 (MQ=255)

                                                                            cggcggCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACggg                                                                                                          <  6:333768/85‑1 (MQ=255)

                                                                            cggcggCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACggg                                                                                                          >  5:333768/1‑85 (MQ=255)

                                                                            cggcggCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGc                                                        >  5:419368/1‑135 (MQ=255)

                                                                              gcggcCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            >  4:382680/1‑113 (MQ=255)

                                                                              gcggcCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  3:382680/113‑1 (MQ=255)

                                                                                gggcAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCttt                                                    <  8:20787/132‑1 (MQ=255)

                                                                                 gcCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCCTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGGGAATGGCCTTGAGACCGATGCGtttt                                                   >  5:318288/1‑135 (MQ=255)

                                                                                              tCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATgg                                       >  1:27213/1‑134 (MQ=255)

                                                                                                   cTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCg                                 <  5:228416/135‑1 (MQ=255)

                                                                                                        tGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCg                            <  1:58727/135‑1 (MQ=255)

                                                                                                        tGAGTCGGGGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCg                            <  3:255370/135‑1 (MQ=255)

                                                                                                         gAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCCGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCgg                           >  2:368494/1‑135 (MQ=255)

                                                                                                         gAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCgg                           >  7:38686/1‑135 (MQ=255)

                                                                                                             cGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGTCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGtt                       >  3:241431/1‑135 (MQ=255)

                                                                                                             cGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGtt                       >  5:275909/1‑135 (MQ=255)

                                                                                                             cGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGtt                       >  1:293102/1‑135 (MQ=255)

                                                                                                                tgtgGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGc                    >  4:181837/1‑135 (MQ=255)

                                                                                                                  tgGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            <  4:446993/77‑1 (MQ=255)

                                                                                                                  tgGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCcgcg                                                                            >  3:446993/1‑77 (MQ=255)

                                                                                                                   gggATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGGCGGCGTTggggtc                 >  3:429579/1‑131 (MQ=255)

                                                                                                                       tGAACTTGGCGTGGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  2:80762/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGCGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  2:200151/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  8:19618/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  2:18433/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  3:334788/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  3:181837/135‑1 (MQ=255)

                                                                                                                       tGAACTTGGCGTAGAGTCTCGCCAGTAAACCGCGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  3:409680/135‑1 (MQ=255)

                                                                                                                        gAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACg             <  6:419368/134‑1 (MQ=255)

                                                                                                                         aaCTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCg                                 >  2:106528/1‑113 (MQ=255)

                                                                                                                         aaCTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCg                                 <  1:106528/113‑1 (MQ=255)

                                                                                                                            ttGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACCCCGCTGCCGTTTTTTGCCGCATGGCAATCGGGCCGGCGGTGGCTTCCACGgcgcg        >  3:250700/1‑135 (MQ=255)

                                                                                                                            ttGGCGTAGAGGCTCGCCAGTAACCCGTGATCCCGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGgcgcg        <  3:70527/135‑1 (MQ=255)

                                                                                                                            tgggCGTAGAGTCTCGCCAGTAAACCGTGAGCACGGGCAGGCCCCTGCAACAGGGCGCGTGCCCGCGAATGGCCTTTACACCGAGGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGgcgcg        <  7:413358/133‑1 (MQ=255)

                                                                                                                              ggCGTAGAGTCTCGCCAGTAAACCGGGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCt      <  5:458450/135‑1 (MQ=255)

                                                                                                                               gCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCt      <  5:357393/134‑1 (MQ=255)

                                                                                                                                 gTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCTTCa   >  2:29595/1‑135 (MQ=255)

                                                                                                                                 gTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGATAAAGGCCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTATTTTGCCGCATGGCAATCCGGACCGCGTTGGGATCCTCGGCGCGCTTCa   >  5:320302/1‑135 (MQ=255)

                                                                                                                                  tAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCTTCAg  <  6:227208/135‑1 (MQ=255)

GCGGCGGCCAACAATGGCGTGACCCGCGACGAGATCAAGGAAGTGCTGATGCAGAGCGCGATCTACTGCGGCATTCCGGCGGCCAATGCCACGTTCCACCTGGCTGAGTCGGTGTGGGATGAACTTGGCGTAGAGTCTCGCCAGTAAACCGTGATCACGGGCAGGCCCCTGCAACAGGGCGCTTGCCCGCGAATGGCCTTTACACCGATGCCTTTTTTTGCCGCATGGCAATCGGGCCGGCGTTGGCTTCCACGGCGCGCTTCAG  >  ACB122/1567347‑1567611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`