Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,716,354 T→C 5.5% L644P (CTG→CCG)  mdtB_1 → Multidrug resistance protein MdtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,716,3540TC 5.5% 216.4 / 3.5 73L644P (CTG→CCG) mdtB_1Multidrug resistance protein MdtB
Reads supporting (aligned to +/- strand):  ref base T (32/37);  new base C (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

CTACGTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAGGCCCGTGCACTGGCCC  >  ACB122/1716220‑1716488
                                                                                                                                      |                                                                                                                                      
cTACGTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCt                                                                                                                                        <  8:167574/135‑1 (MQ=255)
cTACGTGGCCTAGGTGGGCACCGGTGCGCCGCGCGTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCt                                                                                                                                        <  1:384217/135‑1 (MQ=255)
   cGTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACAAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGGTGGCTGAt                                                                                                                                     >  2:133654/1‑135 (MQ=255)
    gTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGCCCGCGAGCGCCTGCGCAGTTGGCTGa                                                                                                                                      >  2:284941/1‑133 (MQ=255)
    gTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGa                                                                                                                                      >  5:81506/1‑133 (MQ=255)
    gTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGGTGGCTgc                                                                                                                                      >  2:208921/1‑132 (MQ=255)
      ggCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAg                                                                                                                                  >  4:272897/1‑135 (MQ=255)
      ggCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAg                                                                                                                                  >  5:292734/1‑135 (MQ=255)
        ccTATGTGGGCACCGGGTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGGCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCa                                                                                                                                <  3:32220/135‑1 (MQ=255)
          tATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGCAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACg                                                                                                                              >  7:83365/1‑135 (MQ=255)
             gtgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATTGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTg                                                                                                                           <  5:371457/135‑1 (MQ=255)
             gtgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTg                                                                                                                           <  3:431049/135‑1 (MQ=255)
              tgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTgg                                                                                                                          >  8:355696/1‑135 (MQ=255)
              tgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTgg                                                                                                                          >  7:56754/1‑135 (MQ=255)
              tgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCCGATCAGCACGGTgg                                                                                                                          >  5:111726/1‑135 (MQ=255)
              tgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCCGATCAGCACGGTgg                                                                                                                          >  5:118366/1‑135 (MQ=255)
              tgGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCACTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATGAGCACGGTgg                                                                                                                          >  1:186288/1‑135 (MQ=255)
                  cACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACca                                                                                                                      <  2:135474/135‑1 (MQ=255)
                  cACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACca                                                                                                                      <  7:464433/135‑1 (MQ=255)
                  cACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGACAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACca                                                                                                                      <  5:45896/135‑1 (MQ=255)
                     cGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACcagca                                                                                                                   >  4:408770/1‑135 (MQ=255)
                     cGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACca                                                                                                                      >  2:31565/1‑132 (MQ=255)
                     cGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACca                                                                                                                      <  1:31565/132‑1 (MQ=255)
                       gTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCAGCGCAGTTGGCTGATCAGCACGGTGGACcagcag                                                                                                                  >  2:74885/1‑134 (MQ=255)
                         tCGCCGCGCTTCTACCTGCCGTTGGACCAGCACCTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGCCCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTc                                                                                                               >  1:421549/1‑135 (MQ=255)
                         tCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGCCCAGCAGTTc                                                                                                               >  2:103360/1‑135 (MQ=255)
                         tCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTc                                                                                                               >  8:344381/1‑135 (MQ=255)
                             cgcgCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGGTGGCTGATCAGCACGGTGGACCCTCAGTTCCCCg                                                                                                           >  4:335669/1‑135 (MQ=255)
                               cgcTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGAc                                                                                                         <  4:111713/135‑1 (MQ=255)
                                        cTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTgcgc                                                                                                   <  4:388635/132‑1 (MQ=255)
                                            cGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCCGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCggg                                                                                            <  6:118366/135‑1 (MQ=255)
                                            cGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCCGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCggg                                                                                            <  6:111726/135‑1 (MQ=255)
                                             gTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCggg                                                                                            >  5:441905/1‑134 (MQ=255)
                                               tGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGGTCGTGGTGCTGGCCAAGTCGCTGGAAGCCCGCGCGCGCCTGCGCAGGTGGGTGAGCAGCCCGGTGGACCCGCAGTTCCCCGACCTGCGCGCCCGGGTcc                                                                                         >  2:189273/1‑134 (MQ=255)
                                                   ccAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCAcgcg                                                                                     >  7:285085/1‑135 (MQ=255)
                                                     aGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGcc                                                                                   >  8:274514/1‑135 (MQ=255)
                                                              cGGCAGCCAGCTTTGCCCAGTTGGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  7:274514/135‑1 (MQ=255)
                                                              cGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  6:441905/135‑1 (MQ=255)
                                                              cGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTGGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  8:442422/135‑1 (MQ=255)
                                                              cGGCAGCCAGCTTTGCCCAGTGCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  6:194228/135‑1 (MQ=255)
                                                              cGGCAGCCAGCTTGGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  3:120357/135‑1 (MQ=255)
                                                              cGGCAGCCAGCTTGGCCCAGTGCGTGGTGCTGGCCAAGTCGATGGACGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTGCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACg                                                                          <  7:240864/135‑1 (MQ=255)
                                                                   gccagcTTTGCCCTGTTCTTGTTGCTGGCCAAGGCGCTGGAAGACCGGGAGCGCCTGCGGAGTTGGATGATCAGCACGGTGGACCTGCAGCTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGgcccg                                                                     <  2:305299/135‑1 (MQ=255)
                                                                   gccagcTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGgcccg                                                                     <  3:377132/135‑1 (MQ=255)
                                                                   gccagcTTTGCCCAGTGCGTGGGGCGGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTTATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGgcccg                                                                     <  3:393233/135‑1 (MQ=255)
                                                                       gcTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGcccgcccg                                                                 >  3:176411/1‑135 (MQ=255)
                                                                             ccccGTTCGTGGGGCGGGCCAAGTCGATGGAACACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCt                                                           <  7:201010/135‑1 (MQ=255)
                                                                                aGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTccc                                                        >  4:59394/1‑135 (MQ=255)
                                                                                aGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTAcc                                                        >  5:455967/1‑135 (MQ=255)
                                                                                 gTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCGCGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTAccc                                                       >  3:208459/1‑135 (MQ=255)
                                                                                     gtggtgCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGACTGGAAAACGGCCCGCCCGTGGGCTACCCGGt                                                    >  1:38817/1‑134 (MQ=255)
                                                                                        gtgtTGGCCAAGTCGTTGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGAGCAGCAGGGTGGACCAGCTGTTCCCGGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAg                                                <  1:74885/135‑1 (MQ=255)
                                                                                        gggCTGGCAAAGGCTTTGGAAGACCGCGAGCGCCTGCGAAGTTGGCTTATCAGCAGGGTGGACCAGCATTTCCACGACCTGCGCGCCCGGGTCACGCGCCTGTAAAACGGCCCGCCCGTGGGCTACCCGGTGCAg                                                <  5:183308/133‑1 (MQ=255)
                                                                                          gcgggCCACGGCGAGGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGAGCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGtt                                              <  6:324103/132‑1 (MQ=255)
                                                                                              gCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCgg                                          >  5:55527/1‑135 (MQ=255)
                                                                                               ccAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCggg                                         >  8:44827/1‑135 (MQ=255)
                                                                                                 aaGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCCCGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTc                                       >  6:308221/1‑135 (MQ=255)
                                                                                                      gATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCCCGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTgg                                  >  7:67605/1‑135 (MQ=255)
                                                                                                      gATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGACAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTgg                                  >  1:373758/1‑135 (MQ=255)
                                                                                                       aTGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTgg                                  <  8:18888/134‑1 (MQ=255)
                                                                                                       aTGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGc                                 <  8:34410/135‑1 (MQ=255)
                                                                                                           acgACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGc                             <  4:72200/133‑1 (MQ=255)
                                                                                                           aaGCCCGCGAGCGCCTGCGCTGTTGGCTGAGCAGCACGGGGGACCAGCAGTGCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGc                             <  1:153644/135‑1 (MQ=255)
                                                                                                           aaGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGc                             <  3:371535/135‑1 (MQ=255)
                                                                                                           aaGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGAGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCACTTCCGGGTCACTGGCGAg                              >  2:231119/1‑134 (MQ=255)
                                                                                                                cgcgAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATc                        <  7:355696/135‑1 (MQ=255)
                                                                                                                cgcgAGCGCCTGCGCAGTGGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATc                        <  6:237522/135‑1 (MQ=255)
                                                                                                                  cgtgcgcCTGCGCAGTTGGCTGATCAGCACGGTGTACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCga                      <  6:393075/132‑1 (MQ=255)
                                                                                                                  cgAGCGCCTGCTCAGTTGGCTGAGCAGCACGGTGGACCAGCTGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCga                      <  1:138270/135‑1 (MQ=255)
                                                                                                                  cgAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCga                      <  6:448733/135‑1 (MQ=255)
                                                                                                                  cgAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCga                      <  4:176411/135‑1 (MQ=255)
                                                                                                                  cgAGCGCCTGCGATGTTGGCTGATCAGCACGGTGGACCAGCAGTGCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCga                      <  1:133118/135‑1 (MQ=255)
                                                                                                                       gcCTGGGCAGTTGGCTGAGCAGCACGGGGGACCCGCAGTTCCCCGCCCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAgg                 <  3:335669/135‑1 (MQ=255)
                                                                                                                       gcCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAgg                 <  4:328586/135‑1 (MQ=255)
                                                                                                                       gcCGGCGCTGTTGGCTGAGCAGCACGGTGCACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAgg                 <  6:292734/135‑1 (MQ=255)
                                                                                                                                      tGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAGGCCCGTGCACTGGccc  >  8:196848/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTACGTGGCCTATGTGGGCACCGGTTCGCCGCGCTTCTACCTGCCGTTGGACCAGCAACTGCCGGCAGCCAGCTTTGCCCAGTTCGTGGTGCTGGCCAAGTCGATGGAAGACCGCGAGCGCCTGCGCAGTTGGCTGATCAGCACGGTGGACCAGCAGTTCCCCGACCTGCGCGCCCGGGTCACGCGCCTGGAAAACGGCCCGCCCGTGGGCTACCCGGTGCAGTTCCGGGTCACTGGCGAGCATATCGAGAAGGCCCGTGCACTGGCCC  >  ACB122/1716220‑1716488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: