Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,763,975	A→C	13.4%	N556T (AAC→ACC)	CJ019_01678 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,763,975	0	A	C	13.4%	165.2 / 3.7	74	N556T (AAC→ACC)	CJ019_01678	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (59/3);  new base C (2/10);  total (61/13)
Fisher's exact test for biased strand distribution p-value = 2.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCGCAAG  >  ACB122/1763847‑1764108                                                                                                                                  |                                                                                                                                        gCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCa                                                                                                                                   >  1:329806/1‑135 (MQ=255) gCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCa                                                                                                                                   >  2:434508/1‑135 (MQ=255) gCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCa                                                                                                                                   >  1:99940/1‑135 (MQ=255) gCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCa                                                                                                                                   >  7:81571/1‑135 (MQ=255) gCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACAGACACCAACGAGTACGACTTCTATCTGCCCGG‑CGGCGTCGCCTGGTACAAGAGCAACGGACa                                                                                                                                   >  2:202771/1‑135 (MQ=255)        gCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGCCTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCACCGGCCCCC‑GCcgg                                                                                                                           >  5:361026/1‑135 (MQ=255)         cGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGCTACAAGAGCAACGCCCACC‑GCcggg                                                                                                                          >  2:367425/1‑135 (MQ=255)            aCGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCcggg‑cgg                                                                                                                      >  6:185471/1‑135 (MQ=255)                   ttCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCt                                                                                                               >  3:31135/1‑135 (MQ=255)                    tCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCCCC‑GCCGGG‑CGGGCTCGCt                                                                                                               >  4:184152/1‑134 (MQ=255)                    tCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCGGGTACCAGAGCCACCGCCCCC‑CCCCGG‑CCGgcgcgca                                                                                                               >  2:148879/1‑133 (MQ=255)                      cACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGaa                                                                                                            >  5:239521/1‑135 (MQ=255)                              aCTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTtcg                                                                                                    >  4:68579/1‑135 (MQ=255)                               cTGGCTTGGGCAGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACATCTATCTGCCCGC‑CGGCTTGGCCTGGTACCAGAGCAACGGCCCCC‑GCCGGG‑CGGGCTCGCTGAACGTGTtcgt                                                                                                   >  1:1059/1‑135 (MQ=255)                                tGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAAGGTGTtcgtc                                                                                                  >  1:169908/1‑135 (MQ=255)                                     tGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCACCGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTg                                                                                             >  3:80023/1‑135 (MQ=255)                                     tGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGACCGTGTTCGTCGAGTg                                                                                             >  8:35961/1‑135 (MQ=255)                                     tGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTg                                                                                             >  8:80426/1‑135 (MQ=255)                                     tGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACCTCTATCTGCCCGG‑CGGCTTGGCCTGGTCCAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTg                                                                                             >  6:428911/1‑135 (MQ=255)                                          cGTTCATGGCGGTGCCAGAGCAGGAGCTGCCCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACCTGTTCGTCGAGTGGcgcg                                                                                        >  7:84452/1‑135 (MQ=255)                                          cGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGGACAAGAGCAACGGCCCCC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGgcg                                                                                          >  3:134636/1‑133 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGCCCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGCACGTGTTCGTCGAGTGGCGCGATGCg                                                                                   >  6:448415/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGACCGTGTTCGGCGAGTGGCGCGATGCg                                                                                   >  6:11615/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCg                                                                                   >  1:141078/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCg                                                                                   >  6:436022/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCg                                                                                   >  4:332817/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCGGAACGTGTTCGTCGAGTGGCGCGCTGCg                                                                                   >  5:29735/1‑135 (MQ=255)                                               aTGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CCGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCg                                                                                   >  1:248126/1‑135 (MQ=255)                                                      tcccAGCGCAGCAGCGCACCCACACCAACCATTACTAATTCTAGCGCCCCGG‑CGGGTGGGGCGGGAAAACGCCCCCCGCCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTg                                                                            <  4:190241/133‑1 (MQ=255)                                                      ggCCAGAGCCGGAGCGCACCCACCCCATCGCGCACCCCTCATAGCGGCCGGG‑CGGCTTGGCCGGGAACAAGACCCCCGCCCACC‑GCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTg                                                                            <  4:191678/134‑1 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑TCCGGG‑CGGGCTCGCTGAACCTGTTCGTCGAGTGGCGCGATGCGGCAATTGGc                                                                          >  7:210404/1‑134 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGc                                                                          >  3:349980/1‑134 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGc                                                                          >  7:336114/1‑134 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGc                                                                          >  7:80983/1‑134 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCg                                                                         >  3:281248/1‑135 (MQ=255)                                                         cAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCg                                                                         >  1:250489/1‑135 (MQ=255)                                                               tttaGCGGAGCGACACGATCGTGAACCAGTTCAATCGCCCCGG‑GGGGTGGGCTGGGTACAAGTGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGt                                                                   <  8:122173/132‑1 (MQ=255)                                                                   gCTGACCGACACCATCGTGTACGACTTCTATCTTCCCGG‑CGGCTTGGCCTGGGACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGCGCGTggcc                                                               >  1:169004/1‑133 (MQ=255)                                                                      gcccGACACCATCGAGTCCGACTTCTAGCTGCCCGG‑CGGCTTGCCCTGGAACACGACCACCGCCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCg                                                            <  4:369605/133‑1 (MQ=255)                                                                      gcccGACACCAGCGAGTCCGACTGCTACCGGCCCGG‑GGGCTGGGCCGGGAACAAGAGCACCGGCCCCC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCg                                                            <  8:377828/133‑1 (MQ=255)                                                                        cggACACCTCCGATTACGATTTATACGTGCCCGG‑GGGTGGGGCTGGGTACAAGACCAACGGCCACC‑GCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCg                                                            <  6:239521/131‑1 (MQ=255)                                                                         cGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCgaga                                                         >  7:84837/1‑135 (MQ=255)                                                                         cGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCgaga                                                         >  5:61712/1‑135 (MQ=255)                                                                         cGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCgaga                                                         >  5:201259/1‑135 (MQ=255)                                                                         cGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCgaga                                                         >  6:67820/1‑135 (MQ=255)                                                                         cGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCgaga                                                         >  5:227488/1‑135 (MQ=255)                                                                          gACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCTGAGCGTGGACCCGagag                                                        >  7:268274/1‑135 (MQ=255)                                                                           acacCAGCGAGTCCGACTTCTCCCGCCCCGG‑CGGGGGGGCGGGGAACACCCCCCCCGCCCCCC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGCgg                                                       <  4:80023/135‑1 (MQ=255)                                                                              ccATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGAGTGGACACGAGAGGTGc                                                    >  8:263553/1‑135 (MQ=255)                                                                              ccAGCGAGCACCACTTCTAGCGGCCCGG‑CGGCTGGGCCGGCAACCAGACCACCGCCCCCC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGc                                                    <  4:158500/135‑1 (MQ=255)                                                                                   gAGTACGACTGCTAGCTGCCCGG‑CGGCTGGGCCTGGTACAAGAGCACCGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGGGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAAc                                               <  2:315233/135‑1 (MQ=255)                                                                                   ctgTACGACTTC‑ACCCGCCCGGCCGGCTGGCCCGGGAACACCACCACCGCCCCCC‑GCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAAc                                               <  4:358112/133‑1 (MQ=255)                                                                                    aGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCaccacc                                              >  2:324929/1‑135 (MQ=255)                                                                                    aGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAAcc                                              >  2:318383/1‑135 (MQ=255)                                                                                    aGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAAcc                                              >  5:227415/1‑135 (MQ=255)                                                                                    aGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAAcc                                              >  1:32287/1‑135 (MQ=255)                                                                                    aGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACCGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGAAGCGGCAATTGGCGGAGCGTGGACCCGCGAGGTGcacca                                                >  5:393547/1‑131 (MQ=255)                                                                                         gACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGc                                           >  2:75195/1‑133 (MQ=255)                                                                                            ttCTATCTGCCCGG‑CGGCTTGGCCTGGTACTAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTCTTCGTCGAGTTGCGCGATGCGGGAATTGGCGGAGCGTGGACCCGAGAGGTGCACACCCTGCAGAGc                                      >  4:251260/1‑135 (MQ=255)                                                                                            ttCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATAGCCGGAGCGTGGACCCGAGAGGTGCACAACCTGCagag                                       >  4:273403/1‑134 (MQ=255)                                                                                             tCTATGTGCCCGG‑GGGCTTGGCCGGGTCCAAGCGCACCGGCCACG‑GCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCa                                     <  8:120556/135‑1 (MQ=255)                                                                                              cTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCa                                     >  2:75776/1‑134 (MQ=255)                                                                                                        gg‑cggcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGAc                          >  7:363371/1‑135 (MQ=255)                                                                                                        gg‑cggcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGAc                          >  7:229696/1‑135 (MQ=255)                                                                                                         g‑cggcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACg                         >  6:339016/1‑135 (MQ=255)                                                                                                            ggcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGCCCCGAGAGGTGCACAACCTGCAGAGCCACACCGAAGACGcc                       >  5:126380/1‑135 (MQ=255)                                                                                                             gcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGCGCACCACCGAAGACGCCa                      >  6:436075/1‑135 (MQ=255)                                                                                                             gcTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCa                      >  3:5294/1‑135 (MQ=255)                                                                                                                tGGCGTGGAACAAGAGCACCGGCCACC‑GCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCg                   <  2:49557/135‑1 (MQ=255)                                                                                                                       gTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCAc            <  4:349980/135‑1 (MQ=255)                                                                                                                          cAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCtt         >  4:79973/1‑135 (MQ=255)                                                                                                                          cAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCtt         >  1:154469/1‑135 (MQ=255)                                                                                                                                cAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCg      >  3:274620/1‑132 (MQ=255)                                                                                                                                cAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCg      >  8:343729/1‑132 (MQ=255)                                                                                                                                 aaCGGCC‑CC‑GCCGGGCCGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCGCAAg  <  1:148879/135‑1 (MQ=255)                                                                                                                                  aCGGCCCCCGGCGGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCGCAAg  <  8:268274/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        GCTTGGTGCGGACGGGGCTTCCACCCTGGACTGGCTTGGGCCGTTCATGGCGGTGCCAGAGCAGGAGCTGACCGACACCATCGAGTACGACTTCTATCTGCCCGG‑CGGCTTGGCCTGGTACAAGAGCAACGGCCACC‑GCCGGG‑CGGGCTCGCTGAACGTGTTCGTCGAGTGGCGCGATGCGGCAATTGGCGGAGCGTGGACCCGAGAGGTGCACAACCTGCAGAGCAACACCGAAGACGCCATCGGCTTCACCTTCCGCAAG  >  ACB122/1763847‑1764108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑