Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,791,684 T→G 22.8% L184R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6840TG22.8% 85.8 / 2.6 61L184R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (35/10);  new base G (4/12);  total (39/22)
Fisher's exact test for biased strand distribution p-value = 4.72e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791553‑1791811
                                                                                                                                   |                                                                                                                               
gCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGgggggccgggg                                                                                                                              >  5:140816/1‑135 (MQ=255)
    aCGCTGCTGCCGTTC‑AGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGTGCTGCGCGGGGCCGGGGCGAgg                                                                                                                         <  4:284531/135‑1 (MQ=255)
       ctgctgCCGTTCGAGGCGTGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCCGGGCCCGCGGGGGCGGGGCGCgggg                                                                                                                       >  3:263104/1‑135 (MQ=255)
            gcCGTTGGAGGCGTGGTGCTACGAAGAGCTGGGTGGGCCAGTGCGTTTTGTCGGCCACCGGCGGGCAGACACCATACCGCTTGACGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCggt                                                                                                                  <  4:263104/135‑1 (MQ=255)
                                    tgcgcggggTGTGCGTGGGGGTTTGGGCGCCCCCCCGCTGCCAGACACAATCCCGGTTCAACCCGCCCCCCCGCCCCGGCGGCCCGCGCGGGCCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCg                                                                                          <  6:4722/129‑1 (MQ=255)
                                    agagCAGGGGGGGCCAGTGCGTTGTGTCGGCCACCCGCGGGCAGACACCATACCGCTGGAAGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCg                                                                                          <  8:430036/135‑1 (MQ=255)
                                      cgCAGGGGGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                        <  2:359813/134‑1 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGTCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  4:179657/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  7:309208/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  4:186095/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  3:190698/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  7:329355/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  8:265177/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  8:387889/1‑135 (MQ=255)
                                         aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCCTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGCTGCCGGGCAGCCGGGGTg                                                                                     >  5:84506/1‑135 (MQ=255)
                                           ggTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTgg                                                                                    >  4:54014/1‑134 (MQ=255)
                                           ggTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGGCGCGCGGGGGCGGGGCGCGGGGCCGGGGGTGGGGGTGCTGCCCGGGCGCCCGGGGGGc                                                                                   >  7:185124/1‑135 (MQ=255)
                                              gtgCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGGCTGGGCGAGGGGGCGGTGGTGGGGGTGGTGCCCGGGCCCCGggggggggca                                                                                >  1:440181/1‑131 (MQ=255)
                                                gCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCCCGCGGGGCCGGGGCGCCGGGCCGGTGGTTGGGGTGTTTCCGGGCCGCCGggggggggga                                                                                >  5:298894/1‑129 (MQ=255)
                                                   aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTatg                                                                           >  8:461206/1‑133 (MQ=255)
                                                   aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  7:457333/1‑135 (MQ=255)
                                                   aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  2:115247/1‑135 (MQ=255)
                                                   aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  7:253290/1‑135 (MQ=255)
                                                   aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  5:368088/1‑135 (MQ=255)
                                                        gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGCGTAGTAGGGCGCt                                                                      >  1:63772/1‑135 (MQ=255)
                                                        gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGGGTTGATGCCGGGCAGCCGGGGTGGTGAAGTAGGGCGCt                                                                      >  7:295689/1‑135 (MQ=255)
                                                              tCGCCCCCCCGCTGGCAGCCACAATCCCGCTCGACGCCGCCCCCCCGCCCGGGGGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                <  2:440977/135‑1 (MQ=255)
                                                                        gCTGGCACCCACCCTCCCGCGTGCAGCCGCCCGCCCGGCCGCGCGGGCCGGGCGGGGCCGGGGGGCGGGGCCGGGGGGGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCt                                                      <  8:329355/135‑1 (MQ=255)
                                                                         cTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGGGGGGCCGGTGGTGGGGGTGATGCCGGGCAGCCGGGGTGGGGGAGTAGGGCGCTTGGGCGCGGTGTTCCtg                                                     >  1:172995/1‑134 (MQ=255)
                                                                         cTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGGTGTTCCTc                                                     >  4:413902/1‑135 (MQ=255)
                                                                                   ccATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGCAGTAGGGCGCTTGGGCGCGCGGTTCCTCGccgccgccg                                           >  1:315137/1‑135 (MQ=255)
                                                                                     aTACCGCTTCACGCCGCCCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAg                                         <  1:282421/135‑1 (MQ=255)
                                                                                            gtcacgCCCACGGCCCGGCCGCGCGTGCGGCGGGGGGCCTGGGGGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCTAGCGCCTCa                                  <  3:54014/130‑1 (MQ=255)
                                                                                              gacgccgccCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGGGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                <  4:9515/132‑1 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 >  2:313993/1‑102 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  6:114775/1‑134 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  8:44333/1‑134 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  6:89748/1‑134 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  1:305249/1‑135 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  6:456730/1‑135 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  1:313993/102‑1 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  6:130563/1‑134 (MQ=255)
                                                                                              gAAGCCGACCGCCCGGCCGCGCGTGCCGCGCAGGGCCTGGGCGACGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAg                                 >  6:352974/1‑134 (MQ=255)
                                                                                               acgccgccCGCCCGGCCGGGCGGGCCGCGCGGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                <  3:72660/132‑1 (MQ=255)
                                                                                               aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                <  8:457333/134‑1 (MQ=255)
                                                                                               aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCCGTGGTGGCGTTGATGCCGGGCAGCCCGGGTGGCGAAGTAGGGCGCTTTGGCGCGCTGTTCCTCGACGCCCCCGAGCGCCACAGc                                >  4:54707/1‑134 (MQ=255)
                                                                                                cgccgccCGCCCGGCCGCGCGGGCCGCGGGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  8:185124/134‑1 (MQ=255)
                                                                                                ccccgcccgcCCGGCCGCGGGGTCGGCGGGGGGCGTGGTGGTGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  5:337140/133‑1 (MQ=255)
                                                                                                accggACCGCCGGGCCGCGCGTGCCGGGCGGGGCCGGGGGGAGGGGGCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  2:151648/131‑1 (MQ=255)
                                                                                                 gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              >  2:47049/1‑134 (MQ=255)
                                                                                                 gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              >  1:369153/1‑134 (MQ=255)
                                                                                                 gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              >  7:17144/1‑134 (MQ=255)
                                                                                                 gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              >  7:24350/1‑134 (MQ=255)
                                                                                                   cGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCCGCcaa                             >  8:236947/1‑133 (MQ=255)
                                                                                                   cGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGGGTTGGTGCCGGGGCGCCCGGGTGGCGCAGTAGGGCGCGTGGGCGCGGGGTTCCTCGCCGCCGCCGCGCGCCACCGcccaaa                           >  2:411001/1‑131 (MQ=255)
                                                                                                        gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCCAACAAGTGc                      >  1:39890/1‑135 (MQ=255)
                                                                                                               cgcgcgTGCCGCGCTGGGCCTGGGGGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGgtgt               <  4:317986/135‑1 (MQ=255)
                                                                                                               cgcgcgGGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGgtgt               <  8:143897/135‑1 (MQ=255)
                                                                                                                 cgcgTGCGGCGGTGGGCGGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGTGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  8:295689/135‑1 (MQ=255)
                                                                                                                 cgcgTGCCGCGCTGGGCGGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  6:233756/135‑1 (MQ=255)
                                                                                                                 cgcgTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  2:305249/135‑1 (MQ=255)
                                                                                                                 cgcgTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  5:130563/135‑1 (MQ=255)
                                                                                                                            ctgggcctgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg  <  4:279748/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
GCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791553‑1791811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑