Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,804,932 C→T 100% S181N (AGC→AAC)  dmlR_4 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,804,9320CT99.1% 237.8 / ‑6.3 73S181N (AGC→AAC) dmlR_4HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (39/33);  minor base G (0/1);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGCTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGC  >  ACB122/1804803‑1805058
                                                                                                                                 |                                                                                                                              
tgCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCGATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCACGCAATTGTGCCGCGCCAGCTCCGACAGGTtgtgt                                                                                                                           <  6:214795/134‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  5:394215/135‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  1:125846/135‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  5:444877/135‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  6:134440/135‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  2:372516/135‑1 (MQ=255)
        cTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   >  3:442599/1‑135 (MQ=255)
          ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGCACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   >  8:166019/1‑133 (MQ=255)
          ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   >  1:209301/1‑133 (MQ=255)
          ggCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   >  2:4291/1‑133 (MQ=255)
            ccACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATa                                                                                                                <  2:75834/134‑1 (MQ=255)
             cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                              <  4:381830/135‑1 (MQ=255)
             cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                              <  6:405197/135‑1 (MQ=255)
             cACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                              <  7:234252/135‑1 (MQ=255)
                    gCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                              >  2:414817/1‑128 (MQ=255)
                    gCAGCGCCAGTTACCCTGTACCCGCTGGCTGAGCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAgc                                                                                                              <  1:414817/128‑1 (MQ=255)
                       gcgcCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATa                                                                                                    <  5:287482/135‑1 (MQ=255)
                       gcgcCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATa                                                                                                    <  5:211848/135‑1 (MQ=255)
                       gcgcCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATa                                                                                                    <  1:397367/135‑1 (MQ=255)
                            aGTTACCGTGAACGCGCTGGCTGAGCTCACGGCCATCCTGGAGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTGAAGATAGGCCg                                                                                               <  1:177349/135‑1 (MQ=255)
                             gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGCCAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCgg                                                                                              >  6:417899/1‑135 (MQ=255)
                             gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGCCAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGACgg                                                                                              >  5:411515/1‑135 (MQ=255)
                             gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCgg                                                                                              >  6:215251/1‑135 (MQ=255)
                             gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCgg                                                                                              >  1:285031/1‑135 (MQ=255)
                             gTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCgg                                                                                              >  5:55653/1‑135 (MQ=255)
                                aCCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCCCCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCg                                                                                            >  6:404692/1‑134 (MQ=255)
                                   cTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCCGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        <  3:428451/135‑1 (MQ=255)
                                   cTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        <  3:435777/135‑1 (MQ=255)
                                   cTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGAGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        <  8:320154/135‑1 (MQ=255)
                                      tACCCGCTGGCTGATCTCACGGCCATCCGGCTGTAGCGCCCTCAAGTCCGAACTCCCCACCATCGAATTGTGCCGCGCCAGCTCCGACTGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCTAAacaca                                                                                     <  2:73952/135‑1 (MQ=255)
                                         ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        <  4:302777/129‑1 (MQ=255)
                                         ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        >  3:302777/1‑129 (MQ=255)
                                         ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAGCACACAg                                                                                  >  5:407054/1‑135 (MQ=255)
                                         ccGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAg                                                                                  >  2:38064/1‑135 (MQ=255)
                                           gctggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGt                                                                                >  7:113374/1‑135 (MQ=255)
                                           gctggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGt                                                                                >  7:267513/1‑135 (MQ=255)
                                             tggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTAc                                                                              <  3:33828/135‑1 (MQ=255)
                                              ggctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACa                                                                             >  1:305646/1‑135 (MQ=255)
                                              cgcagATCACCCGGCAAACCTCATGAAGCGCCCACAACTACGACCTCCCCACCCGGCAATTCTTCCGCGACAACTACGACACGTTCTGAGGCCGGCCATAGCGCTCAAGATACGCAGGCGAAACACACAGGTACa                                                                             >  8:163336/5‑135 (MQ=255)
                                               gctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACAt                                                                            >  5:153858/1‑135 (MQ=255)
                                               gctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACAt                                                                            >  2:244999/1‑135 (MQ=255)
                                               gctgATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACAt                                                                            >  4:307918/1‑135 (MQ=255)
                                                   aTCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATgcgc                                                                        >  4:141409/1‑135 (MQ=255)
                                                        aCGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTtgtgtg                                                                                                                          <  5:159297/80‑1 (MQ=255)
                                                        aCGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTtgtgtg                                                                                                                          >  6:159297/1‑80 (MQ=255)
                                                        aCGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTgg                                                                   <  7:326136/135‑1 (MQ=255)
                                                                  tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGCCAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCgg                                                         >  1:210678/1‑135 (MQ=255)
                                                                  tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   >  3:276742/1‑77 (MQ=255)
                                                                  tgctgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTggccggcc                                                                                                                   <  4:276742/77‑1 (MQ=255)
                                                                     tgTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTa                                                      >  4:55517/1‑135 (MQ=255)
                                                                        aGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGc                                                    <  8:163639/134‑1 (MQ=255)
                                                                           gcCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGcca                                                  >  8:316939/1‑133 (MQ=255)
                                                                                 aaGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        >  1:315884/1‑89 (MQ=255)
                                                                                 aaGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAac                                                                                        <  2:315884/89‑1 (MQ=255)
                                                                                 aaGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAgg                                             >  7:295884/1‑132 (MQ=255)
                                                                                 aaGTCAGAACTCCCCACCAGGCAATTGTGCAGCGCAAGCTCAGACACGTTGTGTGGACTGCCATAGCGCTCAAGATAGGACGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTCGCCACCAgg                                             >  3:116786/1‑132 (MQ=255)
                                                                                       gAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCg                                    >  4:74346/1‑135 (MQ=255)
                                                                                         aCTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGc                                  >  7:308846/1‑135 (MQ=255)
                                                                                          cTCCCCACCAGGCAATGGGGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCAATAGCGCACAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGcc                                 <  5:279946/135‑1 (MQ=255)
                                                                                                accaGGCAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAggcgg                           >  1:190447/1‑135 (MQ=255)
                                                                                                      cAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                     <  3:307918/135‑1 (MQ=255)
                                                                                                      cAATTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGGACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGa                     <  3:33733/135‑1 (MQ=255)
                                                                                                       acttGTGCCGCGCCAGCTCCGACAGGTTGGGTGGCGGCAGATCGCTCTCATGATCGGCCGGCGAAACACACAGGAACAAGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGAc                    <  5:37093/133‑1 (MQ=255)
                                                                                                        aTTGTGCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGAc                    >  4:371584/1‑134 (MQ=255)
                                                                                                          tgtgCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATTCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCACGCGGCCAACACGGa                 >  2:463958/1‑135 (MQ=255)
                                                                                                            tgCCGCGCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATg               >  7:138837/1‑135 (MQ=255)
                                                                                                               cgcgCCAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATgg              >  6:157489/1‑133 (MQ=255)
                                                                                                                   cccgCTCCGACAGGGTGGGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACAAACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt        <  7:399547/132‑1 (MQ=255)
                                                                                                                   ccAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGTGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt        <  5:215251/135‑1 (MQ=255)
                                                                                                                   ccAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGt        <  8:113374/135‑1 (MQ=255)
                                                                                                                    cAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGccacca                                               <  8:28851/95‑1 (MQ=255)
                                                                                                                    cAGCTCCGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGccacca                                               >  7:28851/1‑95 (MQ=255)
                                                                                                                         ccGACAGGTTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGc  >  8:362704/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                              
AGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTGTACCCGCTGGCTGATCTCACGGCCATCCTGCTGTAGCGCCCACAAGTCCGAACTCCCCACCAGGCAATTGTGCCGCGCCAGCTCCGACAGGCTGTGTGGCCGGCCATAGCGCTCAAGATAGGCCGGCGAAACACACAGGTACATGCGCCTTGGCGCCAGGCGGGTAGCCACCAGGCGAGAGTCGGCCAGGCGGCCAAGACGGATGGCCAGGTCCATGC  >  ACB122/1804803‑1805058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑