Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,834,917	T→G	27.3%	T35P (ACC→CCC)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,834,917	0	T	G	27.3%	68.7 / 3.3	54	T35P (ACC→CCC)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (18/18);  new base G (3/12);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 6.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGG  >  ACB122/1834791‑1835049                                                                                                                               |                                                                                                                                     ccgAGCGCAGGGCGATCAACCGCGTGCAAGCGGCGGCCAGGCGCTGCAGAACCGGCGCGTCTAGTTCGGCATTTAGGACGGCGCAGACCACCGCAAAGCCATCTGCCAGGGCGGCGGGGTCGAGGGGCCggcggg                                                                                                                              <  8:37047/135‑1 (MQ=255)    aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGCGTTGGTGATTGATGAAGGCGCAGCCCCCCTCAAAGCCCTCTGGCAGGGCGGCGGGGGCGCGGGTCAGgcgggcgg                                                                                                                           >  4:76988/1‑135 (MQ=255)           ggCGATCACCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTGCGTCATTGATGAAGGCGCAGACCACCTCAACGCCATCTGCCAGGGCGGCGGTGTCGCGGGTCAGGCGGGCGGGCTGGaa                                                                                                                    <  8:70952/135‑1 (MQ=255)           ggCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGaa                                                                                                                    <  3:212392/135‑1 (MQ=255)                  aaCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGGTCGTCATTGATGAAGGCGCCGCCCCCCTCAAAGCCCTCTGCCAGGGCGGCGGTGTCGTGGGGCCGGCGGGCGGGCTGGAAATGGAGc                                                                                                             >  6:442055/1‑135 (MQ=255)                   aCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGGGGTCGTCATTGATTAAGGCGCAGCCCACCTCAAAGGCATCTGGCCGGGCGGCGGGGGCGCGGGGCGGGCGGGCGGGCGGGGAATggcgct                                                                                                            >  3:321982/1‑130 (MQ=255)                   aCCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTGGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCt                                                                                                            >  4:61532/1‑135 (MQ=255)                                     ccAGGCGCTGCAGCACCGGCGCGGGGAGTTAGTCATTGATGAAGGCGCAGGCCACCTCAAAGCCATCTGCCAGGGCGGCGGGGTCGAGGGTCCGGCGGGCGGGCGGGAAATGCAGCTCCAGGGCGGTGCCGCCgg                                                                                          >  6:400754/1‑135 (MQ=255)                                      ccggCGCTGCAGCACGGGCGCGGCGAGTTCGCCATTGATGACGGCGCACACCACCTCAACCCCAGCGGCCCGGGCGGCGGGGGCGAGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGc                                                                                         <  4:291369/133‑1 (MQ=255)                                       aGGCGCTGCAGCACCGGCGCGGGGAGTTGGGGATTGGTGAAGGCGCAGGCCACCGCAAAGCCCTCTGCCCGGGCGGCGGGGTCGAGGGTGAGGCGGGGGGGGGGGAAATGCAGCTCCCCGGCGGTGCCGCCGGcc                                                                                        >  4:84518/1‑134 (MQ=255)                                                cgcaCCGGCGCGTCGTTTGCGTATTTGATTACGGCGCAGACCACCACAAACCCATCTGCGAGGGGGGGGGTGTCGAGGGCCCGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  7:359484/134‑1 (MQ=255)                                                agccCCGGCGGGTCGAGTGCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCGGCCCGGGCGGCGGTGGCGCGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  6:410746/135‑1 (MQ=255)                                                agcaCGGGCGGGTCGTGTGCGTCTTTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCCGGGCGGCGGTGTCGTGGGGCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  6:403899/135‑1 (MQ=255)                                                agcaCGGGCGCGTCTCGTTCGCCATTGATGACGGCTCACACCACCTCAAAGCCATCTGCCCGGGCGGCGGGGGCGAGGGGCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  3:306408/135‑1 (MQ=255)                                                agcaCCGGCGCGGCGAGTGCGTCATGGCTGACGGCGCCGACCCCGTCACAGCCATCTGCCCGGGGGGCGGTGTCGTGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  8:304864/135‑1 (MQ=255)                                                 gacagggCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCTGGGGGGGGGTGTCGTGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                               <  1:375493/129‑1 (MQ=255)                                                       gcgcGTCGACTTCGTCATTGATGAAGGCGCAGACCCCCACAAAGCCATATGCCAGGGCGGCGGTGTCGAGGGGCCGGCGGGCGGGCTGGAAATGCACCTCCAGGGCGGTGCCGCCGGCCGCCATGGTGAAGCttt                                                                        >  8:161590/1‑135 (MQ=255)                                                       gcgcGGGGAGTTCGTCATTGATGAAGGCGCAGACCACCTCACCGCCATCTGCCTGGGCGGCGGTGTCGAGGGGCAGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCttt                                                                        <  7:291637/135‑1 (MQ=255)                                                                    ttaaTTGATCACGGCCCAGACCACCTAAACCCCATCTGCCCGGGGGGGGGTGGCGCGGGCCTGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGt                                                            <  8:97176/131‑1 (MQ=255)                                                                    gTCTTTGTTGAAGCCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGGGGTGTCGCGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGt                                                            <  2:30233/134‑1 (MQ=255)                                                                    gTCATTGATTAATGCGCAGACCAGCTCAAACCCATCTCCCAGGCCGGCGGAGTCGAGGGACAGGCGGGCCGGCTGGAAATGCAGCTCCAGGGCTGTGCCGCCGGCACCCTTCGAGAAGCTTTCCTGATcctgtct                                                           >  2:375493/1‑129 (MQ=255)                                                                      cATTGATGACGGCCCAGACCACCTCAACGCCATGTGCCAGGGCGGCGGTGTCGAGGGTCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgct                                                         <  8:64167/135‑1 (MQ=255)                                                                      atttgttgATGGCGCAGACCACCTCAAAGCCATCTGCCTGGGGGGGGGTGTGTTGGGTCTGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgct                                                         <  3:61532/133‑1 (MQ=255)                                                                        ttGATTACGGCTCATACCACAACAAAGCCATCGCCCCGGGGGGCGGTGTCTGGGGTCAGGGGGGGTGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctgg                                                       <  1:315403/135‑1 (MQ=255)                                                                           atgaAGGCGCAGACCACCTCAACGCCATCTGCCAGGGCGGCGGGGGCGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggct                                                     <  7:272818/134‑1 (MQ=255)                                                                            tgaAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGGGTCGAGGTGACGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggctg                                                    <  4:458733/134‑1 (MQ=255)                                                                            tgaACGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTAGACGGTCAGGCCGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCCGCCGCCTAGCTGAAGCTATCCTGATCCTAGTCCTGgctgc                                                   >  6:72534/1‑135 (MQ=255)                                                                             gaAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGgctgct                                                  <  8:317960/135‑1 (MQ=255)                                                                                ggCGCAGACCACCTCAATGCAAGATGCCAGGGGGGGGGTGGGGTGGGTCAGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGaa                                               <  5:400754/135‑1 (MQ=255)                                                                                 gcgcAGACCACCTCAAACCCATCGGCCAGGGCGGGGGTGGCGAGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGaac                                              <  3:184354/135‑1 (MQ=255)                                                                                     aGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAacaaca                                          >  7:285897/1‑135 (MQ=255)                                                                                       accaccTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAgg                                        >  2:95649/1‑135 (MQ=255)                                                                                        ccaccTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAggg                                       >  2:219071/1‑135 (MQ=255)                                                                                           ccTAAAAGCCATCTGCCAGGGCGGGGGGGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgc                                    <  6:76490/135‑1 (MQ=255)                                                                                            cTCAAAGCCATCTGCCAGGGCGGCGGTTTCTAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgcg                                   <  1:30845/135‑1 (MQ=255)                                                                                               aaaGCCATCTGCCAGGGAGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCAt                                >  8:298555/1‑135 (MQ=255)                                                                                                 cgctttCTGCCTGGGGGGGGGTGTCTAGGGGCGGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                              <  6:70717/130‑1 (MQ=255)                                                                                                 aGCCATCTGCCAGGGCGGGGGTGTCGTGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                              <  3:74297/135‑1 (MQ=255)                                                                                                 aGCCATCGGCCAGGGGGGGGGTGTCGAGGGTCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                              <  4:429529/135‑1 (MQ=255)                                                                                                  gCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGt                             >  2:439102/1‑135 (MQ=255)                                                                                                   ccATCTGCCCGGGCGGGGGTGTCGAGGGTCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc                            <  5:164662/135‑1 (MQ=255)                                                                                                    cATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGATGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCg                           >  3:458321/1‑135 (MQ=255)                                                                                                    cATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCGTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGCACAACAGGGCGCGCATGGTc                            >  8:14695/1‑134 (MQ=255)                                                                                                      gcggCCTGGGCGGGGGTGGCGCGGGTCCGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGaa                         <  8:285897/132‑1 (MQ=255)                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc                      >  2:53587/1‑135 (MQ=255)                                                                                                         gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc                      >  7:341505/1‑135 (MQ=255)                                                                                                           ctgggCGGGGGTGGGGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCt                    <  3:127842/133‑1 (MQ=255)                                                                                                            aGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGCAGCTTTCCTGATCGTAGTGCTGGCGGCTGAACAACAGGGCGCGCATGGTCGAACTCCtt                   >  8:166787/1‑135 (MQ=255)                                                                                                                  ggggTGGCGTGGGTCCGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAAt             <  4:457015/133‑1 (MQ=255)                                                                                                                   cggTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAAt             >  7:336486/1‑134 (MQ=255)                                                                                                                     gtgtCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCa          >  2:39341/1‑135 (MQ=255)                                                                                                                           aGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCtt    <  1:219071/135‑1 (MQ=255)                                                                                                                            gggTCTGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTtg   <  7:28771/135‑1 (MQ=255)                                                                                                                             ggTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTtgg  >  5:99995/1‑135 (MQ=255)                                                                                                                               |                                                                                                                                     CCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGG  >  ACB122/1834791‑1835049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑