Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,934,465 C→G 17.0% intergenic (‑156/‑54) CJ019_01849 ← / → CJ019_01850 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,934,4650CG17.0% 193.6 / 8.6 92intergenic (‑156/‑54)CJ019_01849/CJ019_01850hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/46);  new base G (14/2);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 5.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAGGCT  >  ACB122/1934337‑1934596
                                                                                                                                |                                                                                                                                   
gTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTgtgt                                                                                                                               >  7:451698/1‑135 (MQ=255)
gTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTgtgt                                                                                                                               >  3:55704/1‑135 (MQ=255)
     aTAGTAGCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGTGGTGTTggtg                                                                                                                          >  5:310446/1‑132 (MQ=255)
      tagtagCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAg                                                                                                                          >  8:318439/1‑134 (MQ=255)
            cccGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGGTGGAGGTAgagg                                                                                                                   >  5:308388/1‑133 (MQ=255)
            cccGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTTCATGAAcatg                                                                                                                   >  6:170367/1‑135 (MQ=255)
              cGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAgatgat                                                                                                                 <  5:420187/135‑1 (MQ=255)
              cGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAgatgat                                                                                                                 <  1:69201/135‑1 (MQ=255)
              cGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAgatgat                                                                                                                 <  4:117165/135‑1 (MQ=255)
              cGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAgatgat                                                                                                                 <  7:174398/135‑1 (MQ=255)
              cGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGACGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAgatgat                                                                                                                 <  5:352551/135‑1 (MQ=255)
                  gcCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGTTGGCGGAAGATGGTGCaa                                                                                                             >  5:162019/1‑135 (MQ=255)
                    cAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCCGGTAGCTGATGCaaac                                                                                                           >  1:280519/1‑133 (MQ=255)
                     aaaCCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGGTGGAGGAAGATTATGCAAtag                                                                                                          >  5:445517/1‑133 (MQ=255)
                      aaCCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAAtctc                                                                                                         <  7:318439/135‑1 (MQ=255)
                         cTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCaa                                                                                                      <  4:55704/135‑1 (MQ=255)
                         cTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCaa                                                                                                      <  1:441265/135‑1 (MQ=255)
                         cTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCaa                                                                                                      <  6:323854/135‑1 (MQ=255)
                              gCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCATGAAGATGATGCAATCTCCAAGccc                                                                                                  >  1:137285/1‑134 (MQ=255)
                              gCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCACGAAGATGATGCAATCTCCAAGAcc                                                                                                  >  4:190129/1‑134 (MQ=255)
                               cATGCAAGGATCGCGCATGGCGAGGTAGGCCGATGGCGCTAATGGCATTGTGCTGTCGCATATGGTGCGGGTGCAGGTCGCAGCTGGATCAGGTGGTTTTGTGTTGCAGGAAGATTATGCAATCTCCAAGACCTg                                                                                                <  4:165056/135‑1 (MQ=255)
                                    aaGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTTGGTTGCCGGAAGTTGATGCAATATCCAAGCCCGGACGAg                                                                                           >  8:219204/1‑135 (MQ=255)
                                     aGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGATGCAATCTACAAGACCTGACggcg                                                                                          >  5:120763/1‑132 (MQ=255)
                                      ggATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGGTGCAGGAAGCTGATGCAATCTCCAAGCCCGGACGAggg                                                                                         >  3:120078/1‑135 (MQ=255)
                                      ggATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAggg                                                                                         >  3:124738/1‑135 (MQ=255)
                                      ggATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTGGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGCCGAggg                                                                                         >  4:82449/1‑135 (MQ=255)
                                      ggATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGGTGGAGGCAGCTGATGCAAAATACAAGACCAGACGAgcg                                                                                         >  5:344865/1‑133 (MQ=255)
                                           gcgcAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGTTGGGGTGGCGGGAGATTCTGCACTCTGCACGACCGGTCGCGTGGATgg                                                                                    >  2:379304/1‑134 (MQ=255)
                                               aGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGGAGGAAGATGATGGAATCTCCAAGACCTGACGACGGGTTGCGTTg                                                                                >  8:250679/1‑135 (MQ=255)
                                               aGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGCTGCAATCTCCAAGACCTGACGAGGGGATGCATTg                                                                                >  8:23194/1‑135 (MQ=255)
                                               aGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCtgtg                                                                                >  2:33466/1‑135 (MQ=255)
                                                ggCCGAGGTAGGCCGATGTCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGTTCGTCTTGTGGTGCTGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGa                                                                               <  7:383498/135‑1 (MQ=255)
                                                  ccGAGGTAGGCCGATGGCGCTTATGGGATTGTGCTGTGGCATATGGGGCGGGTGCTGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGa                                                                               <  7:26400/133‑1 (MQ=255)
                                                        tAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGTTGGAGGGAGATGCTGGCATCTCCCAGCACGGGCGAGGGGGTGGGTTGTTgggcact                                                                       >  3:294160/1‑130 (MQ=255)
                                                         aGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGGTGCAGGAACGAGTAGCCAGCTCCCAGCCCGGACGAGGGGATGCTTAGATGGCAAcgt                                                                      >  5:196034/1‑133 (MQ=255)
                                                                  gcgcTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGTTGTGTTGGAGGAAGCTGATGCAATCTCCAAGTCCGGTCGAGGGGGTGCTTTGATGGGGACTTTGGAGaaaa                                                             >  4:38585/1‑135 (MQ=255)
                                                                    gcTAATGGCATTTGGGTTTCGCTTTTGGGGCTGGGGCAGGTCGAAGGTGGTTCTGTTCGTCTTGTGTTGCAGGACTATTTTCAAATCTCAAAGACGTGACGAGGTTATGCTTTGTTGGCAACTTTGAAGAAATTg                                                           <  4:450324/135‑1 (MQ=255)
                                                                    gcTAATGGCATAGTGCTGTGGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGAAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATg                                                           <  7:23194/135‑1 (MQ=255)
                                                                    gcTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCTGGTCGCAGCTGGATCAGGTCGGCTTGTGTTGAAGGAAGATGCTGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATg                                                           <  7:453022/135‑1 (MQ=255)
                                                                        aTGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGtt                                                                                                                                   >  8:170875/1‑59 (MQ=255)
                                                                        aTGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCtt                                                                                                                                   <  7:170875/59‑1 (MQ=255)
                                                                        aTGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGGTGCTTTGATGGGACCTTTGAAGAAAATGCGAc                                                       >  7:153280/1‑135 (MQ=255)
                                                                           gCATAGGGCTGGCGCATATGGGGGGGGGGCAGGTCGCAGCTGGAGCAGGTCGGCTGGTGTTGCAGGACGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGtt                                                    <  6:306245/135‑1 (MQ=255)
                                                                            cATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGtt                                                    <  8:451698/134‑1 (MQ=255)
                                                                             aTAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTc                                                  <  2:69615/135‑1 (MQ=255)
                                                                             aTAGTGCGGTCGCATATGGGGCGGGTGCAGGGCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTc                                                  <  8:349924/135‑1 (MQ=255)
                                                                                 tGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTgg                                              >  2:259459/1‑135 (MQ=255)
                                                                                 tGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTTTGTTTGCGTTATAGGATGCAATCTCCAAGACCGGACGCGGGGATGCTTTTATGGCAACATTGAAGAAAAAGCGCCCTTTACttg                                              >  6:310855/1‑133 (MQ=255)
                                                                                 tGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGGAGGAAGATGATGGAATCGCCAAGACCGGACGCGGGGCTGCGTTGTTGGGAACTTTGAAGAAAATGCGACGGTTCCTgg                                              >  8:180767/1‑135 (MQ=255)
                                                                                  gCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTggg                                             <  7:454335/135‑1 (MQ=255)
                                                                                    tGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGTTGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCg                                           >  8:251551/1‑135 (MQ=255)
                                                                                     gTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCGTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGt                                          >  7:357325/1‑135 (MQ=255)
                                                                                     acagCATATGGGGCGGGTGCAGGTCGCAGCTGGATGAGGTCGTCTTGTGTTGCATGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGt                                          <  8:313302/132‑1 (MQ=255)
                                                                                       cGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTgg                                        >  8:97370/1‑135 (MQ=255)
                                                                                       cGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGGCGTGTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTgg                                        <  2:259127/135‑1 (MQ=255)
                                                                                        gattttGGGGCGGGGGCCGTGCGCAGCTGTAGCAGGTCGTGTTGTGTTGATGTAATATGATGCAATCGCCAACACCTGTCGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTggg                                       <  5:5843/130‑1 (MQ=255)
                                                                                        gCATCTGGGGCGGGTGCAGGTCGCAGCGGGATCAGGGCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTggg                                       <  6:232745/135‑1 (MQ=255)
                                                                                        gCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTggg                                       <  7:345549/135‑1 (MQ=255)
                                                                                         cATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGCCGAGGGGATGCGTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGc                                      >  7:466715/1‑135 (MQ=255)
                                                                                           tatGGGGCGGGTGCAGGTCGCAGCTGGTTCAGGTCGTCTGGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCAc                                    <  8:222830/135‑1 (MQ=255)
                                                                                            atGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCAcc                                   <  7:386516/135‑1 (MQ=255)
                                                                                            atGGGGCGGGTGCAGGGCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTTAAGAAAATGCGACGTTTCCTGGGCGTGGGCAcc                                   <  5:195150/135‑1 (MQ=255)
                                                                                            agggggCGGGTGCCGGGCGCCGCTGGATCAGGGCGGCTTGTGTGGCCGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCAcc                                   <  6:373402/133‑1 (MQ=255)
                                                                                            agagaccGGGTGCAGGTCGCAGCTGCATCAGGTCGTCTTGTGTGGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCAcc                                   <  5:465595/129‑1 (MQ=255)
                                                                                             tGGGGCGGGTGCAGTTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCAcc                                   <  6:308388/134‑1 (MQ=255)
                                                                                              ggggCGGGTGCAGGTCGCAGCTGGATCAGGTCGTGGTGTGTTGGAGGAAAATGATTCAATCTCCCAGCCCGGCCGAGGGGATGGGTTGCTGGCCACATTGAAGAAAATGCGACGGTTCCTGGGCGTGGGGCCCGc                                 >  5:72354/1‑135 (MQ=255)
                                                                                              ggggCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGc                                 >  7:128313/1‑135 (MQ=255)
                                                                                              ggggCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTTGAGGAAAATTATTGAAAAACCAAAACACGCCGCAGGGGTGCGTTTATTGCAACTTTGAATAAAAAGCCACCTTTACGGGGCGGGGGGCCCGc                                 >  6:256426/1‑135 (MQ=255)
                                                                                                 gCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAAACTCCAAGACCGGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGGGACGTTTCCTGGGCGTGGGCCCCGCCCt                              >  6:232544/1‑135 (MQ=255)
                                                                                                   gggTGCAGGGCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTgg                            <  2:363354/135‑1 (MQ=255)
                                                                                                   gggTGCAGGGCGCAGCTGGAGCTGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTgg                            <  8:148386/135‑1 (MQ=255)
                                                                                                      tGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGCTGCGTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCGGGTaa                         >  3:302710/1‑135 (MQ=255)
                                                                                                       gCAGGTCGCAGCTGGATCAGGTCGTGTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTTCTTGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTTAt                        >  5:157723/1‑135 (MQ=255)
                                                                                                       gCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGGAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATTCGACGTTTACTGGGCGTGGGCGCCCCCCAggcggt                        >  7:313302/1‑131 (MQ=255)
                                                                                                       gCAGGTCGCAGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGATGCCATCTCCAAGCCCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGTGCGTGGGCACCGCCCAGGTAAt                        >  6:442188/1‑135 (MQ=255)
                                                                                                         aGGTCGCCGCTGGATCAGGTCGTCGTGTGTTGCAGGAAGATGATGCAATCTACAAGCACAGACGAGGGGATGGTTTGATGTCCACGTTGAAGACAATTCGAAGTTTGCTGGGCGTGGGCGACGCCCTGGAAATgg                      >  4:450987/1‑135 (MQ=255)
                                                                                                          gggcGCAGCTGGTTCAGGTCGTCTTGTGTTGCAGGAAGATGCGGCAATCTCCACGCCCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGc                     <  3:441320/132‑1 (MQ=255)
                                                                                                          ggTCGCAGCTGGATCAGGTCGTGTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGGCGAGGGGGTGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATgg                      >  2:79530/1‑134 (MQ=255)
                                                                                                               ctgCTGGATCCGGTCGGCTTGGGTTGCCGGAAGATGATGCAAGCTCCCAGCCCTGACGAGGGGAGGCTTGGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGa                <  4:245045/133‑1 (MQ=255)
                                                                                                               cAGCTGGATCCGGTCGTCTTGTGTTGCAGTAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGa                <  5:416301/135‑1 (MQ=255)
                                                                                                               cAGCTGGATCAGGTCGTCTTGTGTGGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGa                <  4:396359/135‑1 (MQ=255)
                                                                                                               cAGCGGGATCAGGGCGTCTTGGGTTGCAGGAAGATGCTGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGa                <  6:310446/135‑1 (MQ=255)
                                                                                                                    ggATCAGGTCGTCTTGTGTGGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  7:397847/135‑1 (MQ=255)
                                                                                                                    ggATCAGGTCGTCTGGTGTTGCAGGAAGAGGATGCAAGCGCCAAGCCCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAACTGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  5:268279/135‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  7:300295/133‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  2:143034/133‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  1:153234/133‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  1:111010/133‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTg          <  8:128313/134‑1 (MQ=255)
                                                                                                                      aTCAGGTCGTCTTGGGTTGCTGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGAGGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  1:400852/133‑1 (MQ=255)
                                                                                                                      aTCAGGGCGTCTTGTGTTGCAGGAAGAGGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCt           <  1:145321/133‑1 (MQ=255)
                                                                                                                          gggcggcTTGTGTGGCAGGAAGATGCTGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAg     <  5:285165/129‑1 (MQ=255)
                                                                                                                          ggTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAg     <  6:162019/135‑1 (MQ=255)
                                                                                                                            gcgtcTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAGGc   <  8:324725/134‑1 (MQ=255)
                                                                                                                             cgtcTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAGGCt  >  8:50082/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                   
GTTAGATAGTAGCCCGGCGCCAAACCTTTGGCATGCAAGGATCGCGCAGGCCGAGGTAGGCCGATGGCGCTAATGGCATAGTGCTGTCGCATATGGGGCGGGTGCAGGTCGCAGCTGGATCAGGTCGTCTTGTGTTGCAGGAAGATGATGCAATCTCCAAGACCTGACGAGGGGATGCTTTGATGGCAACTTTGAAGAAAATGCGACGTTTCCTGGGCGTGGGCACCGCCCTGGTAATGGCCATGAGCGCTGCACAGGCT  >  ACB122/1934337‑1934596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: