Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,947,503 G→A 5.4% R257H (CGC→CAC)  CJ019_01861 → Polyphosphate:AMP phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,947,5030GA 5.4% 252.2 / 3.0 76R257H (CGC→CAC) CJ019_01861Polyphosphate:AMP phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base A (2/2);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

GGGTAGACCCGAATTACCGCAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACGCCCTGGTG  >  ACB122/1947371‑1947633
                                                                                                                                    |                                                                                                                                  
gggTAGACCCGAATTACCGCAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGCCGACCGCa                                                                                                                                  >  7:192280/1‑135 (MQ=255)
      aCCCGAATTACCGCAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACACTCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGc                                                                                                                            >  8:307806/1‑135 (MQ=255)
       cccGAATTCCCGCAGCCTGGGGGTGGGGCGCATTCTGCTGGAACGCCTGCACGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCt                                                                                                                           <  4:32430/135‑1 (MQ=255)
           aaTTACCGCAGCCGGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCACGGGCAAGCACCAGGGCAACGGGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGgc                                                                                                                       <  8:206373/135‑1 (MQ=255)
              tACCGCAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCCCAGCCTGCTTGGCGcc                                                                                                                    >  5:65950/1‑135 (MQ=255)
                   cAGCCTGGCGGTGGGGCGCTTTCTGCTGGAAAGCCTGCACGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGa                                                                                                               <  7:4571/135‑1 (MQ=255)
                   cAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCACGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGa                                                                                                               <  7:11500/135‑1 (MQ=255)
                   cAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTgg                                                                                                                >  7:231280/1‑134 (MQ=255)
                   cAGCCTGGCGGTGGGGCGCAGTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGa                                                                                                               <  8:215168/135‑1 (MQ=255)
                    aGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCCCCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGCCGACCGCAGCCTGCTTGGCGCCCTGGAc                                                                                                              >  3:265691/1‑135 (MQ=255)
                        tGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGa                                                                                                          <  7:26586/135‑1 (MQ=255)
                        tGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGa                                                                                                          >  7:377183/1‑135 (MQ=255)
                        tGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGa                                                                                                          <  1:50490/135‑1 (MQ=255)
                        tGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGa                                                                                                          >  4:157692/1‑135 (MQ=255)
                             gTGGGGCGCATTCTGCTCTACCGCCTGCTAGCCGCGCTGGCCCACAATCCCAAGGTAAAGCACCAGGCCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTg                                                                                                     <  6:8297/135‑1 (MQ=255)
                                 ggCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCACGGGCAAGCACCAGGGCAACGTGGCGCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGcc                                                                                                 <  4:68155/135‑1 (MQ=255)
                                   cgcATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCt                                                                                                >  5:244362/1‑134 (MQ=255)
                                         tctgcggAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGCCATGACCTTGCGCCTGGACAAg                                                                                         >  7:412281/6‑135 (MQ=255)
                                           gctgGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCAGCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGc                                                                                       <  7:75181/135‑1 (MQ=255)
                                             tgGACAGCCTGCAAGCGGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGGAAGGTGGGTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGc                                                                                       <  4:239084/133‑1 (MQ=255)
                                             gggAAAGCCTGCACGCCGCGCGGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGc                                                                                       <  1:298/132‑1 (MQ=255)
                                                   gCCTGCAAGCCGCGCTGGCCCACAATCACAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATc                                                                               <  4:84212/135‑1 (MQ=255)
                                                     gtGCAAGCCGCGCGGGCCCACAATCCCACGGGCTAGCACCTGGGCAACGGGGCTCGATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAg                                                                             <  7:124932/134‑1 (MQ=255)
                                                     ctgaatgCCGGGGTGGCCAAAAATACAAAGGGCAAGAACCTTGGAAAGGTGGCTCCATTGGTCCTCAGCTTCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAg                                                                             <  6:65950/129‑1 (MQ=255)
                                                     cTGCAAGCCGCGCTGGCCCACAATCCCACGGGCAAGCACCAGGGCAAGTTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAg                                                                             <  6:351093/135‑1 (MQ=255)
                                                     cTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAg                                                                             <  3:228256/135‑1 (MQ=255)
                                                       gCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGa                                                                           >  7:75272/1‑135 (MQ=255)
                                                         aaGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGa                                                                           >  3:75050/1‑133 (MQ=255)
                                                          acccGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGGGGCTCCATTGGGCCGCAGCAGCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACa                                                                        <  7:124158/133‑1 (MQ=255)
                                                                  tGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTAGCCGGAACAGTTGATCa                                                                >  6:248381/1‑135 (MQ=255)
                                                                                 aGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTc                                                 >  5:46076/1‑135 (MQ=255)
                                                                                   ggCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTg                                               >  3:66263/1‑135 (MQ=255)
                                                                                      aaGCACCATGGCAACGTGGCTCCATAGGGCCGCAGCATCGACGACCGCAGCATGCTTGCCGCCCTGGCCATGACCTTGCGCATGGACAAGGCCGCCTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggcc                                            >  3:122733/1‑135 (MQ=255)
                                                                                       aGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGCCGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggccg                                           >  2:28057/1‑135 (MQ=255)
                                                                                         cACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggccggc                                         >  6:194196/1‑135 (MQ=255)
                                                                                          aCCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCCCCGAACAGGCGCGTCTggccggcc                                        >  7:125244/1‑135 (MQ=255)
                                                                                          aCCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGTCGCGTCTggccggcc                                        >  2:214292/1‑135 (MQ=255)
                                                                                          aCCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggccggcc                                        >  8:178584/1‑135 (MQ=255)
                                                                                           ccAGGGCAACGTGGCTCAATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggccggcc                                        >  1:351883/1‑134 (MQ=255)
                                                                                            cAGGGCAACGTGGCGCCATTGGGCCGCAGCTGCGACGACCGCAGCCTGCTTGGCGCCGTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAAGAGTTGATCACCGAACAGGCGAGTCTGGCCGGCctg                                      <  4:265691/135‑1 (MQ=255)
                                                                                                gCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCtgctgc                                  >  4:340401/1‑135 (MQ=255)
                                                                                                gCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCtgctgc                                  >  4:421559/1‑135 (MQ=255)
                                                                                                 cAACGTGGCTCGATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggcc                                            <  2:378051/124‑1 (MQ=255)
                                                                                                 cAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTggcc                                            >  1:378051/1‑124 (MQ=255)
                                                                                                 cAACGTGGCTCCATTGGGCCGCAGCATCGACGACCACAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACa                                                                        <  2:29898/96‑1 (MQ=255)
                                                                                                 cAACGTGGCTCCATTGGGCCGCAGCATCGACGACCACAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACa                                                                        <  2:21830/96‑1 (MQ=255)
                                                                                                 cAACGTGGCTCCATTGGGCCGCAGCATCGACGACCACAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACa                                                                        >  1:29898/1‑96 (MQ=255)
                                                                                                 cAACGTGGCTCCATTGGGCCGCAGCATCGACGACCACAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACa                                                                        >  1:21830/1‑96 (MQ=255)
                                                                                                      tGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACa                            >  3:50985/1‑135 (MQ=255)
                                                                                                       ggCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACaa                           >  1:166751/1‑135 (MQ=255)
                                                                                                       ggCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACaa                           >  5:287776/1‑135 (MQ=255)
                                                                                                       ggCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACaa                           >  3:54196/1‑135 (MQ=255)
                                                                                                       ggCTCCATTGGGCCGCAGCATCGAAGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGaaaa                           >  1:170688/1‑135 (MQ=255)
                                                                                                         cgccATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAg                          <  4:232286/132‑1 (MQ=255)
                                                                                                         cTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAgc                         <  7:178584/135‑1 (MQ=255)
                                                                                                         cTCCATTGGGCAGCAGCAACGACGACCGCATCCTGCTTGGCGCCCTTGACATGACCTTGCGCCTGGACAAAGCCGACTATCATGAACAGTTTATCAACGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAgc                         <  2:377122/135‑1 (MQ=255)
                                                                                                            cATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCa                      >  5:115533/1‑135 (MQ=255)
                                                                                                            cATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCa                      >  1:353442/1‑135 (MQ=255)
                                                                                                            cATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCa                      >  5:115532/1‑135 (MQ=255)
                                                                                                            cATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCa                      >  7:285003/1‑135 (MQ=255)
                                                                                                             aTTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCAt                     <  8:377183/135‑1 (MQ=255)
                                                                                                                 ggCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATgcgc                 >  6:154842/1‑135 (MQ=255)
                                                                                                                   ccgccgCAGCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCg               <  1:357127/135‑1 (MQ=255)
                                                                                                                   ccGCAGCTGCGCCGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCCCCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCg               <  7:107180/135‑1 (MQ=255)
                                                                                                                   ccGCAGCAGCGACGACCGCAGCCTGCTGGGCGCCCTGGACATGCCCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCg               <  1:98186/135‑1 (MQ=255)
                                                                                                                      cagcaTCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCa            >  2:406274/1‑135 (MQ=255)
                                                                                                                        gcagccacgacCGCCGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACg          <  4:107392/129‑1 (MQ=255)
                                                                                                                         caTCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGc                                                                                       >  2:419540/1‑57 (MQ=255)
                                                                                                                         caTCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGc                                                                                       <  1:419540/57‑1 (MQ=255)
                                                                                                                         caTCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACGc         >  2:301135/1‑135 (MQ=255)
                                                                                                                            ggacgacCGCAGCCTGCTTGGCGGCCTGGACATGACGTTGCGCCTGGACAAGGCGGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACGCCCt      <  2:167483/134‑1 (MQ=255)
                                                                                                                              acgacCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGcgcg                                                   >  7:319869/1‑88 (MQ=255)
                                                                                                                              acgacCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGcgcg                                                   <  8:319869/88‑1 (MQ=255)
                                                                                                                              acgacCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACACGCGCATGCGCCGGCACGCCCtgg    >  6:269226/1‑135 (MQ=255)
                                                                                                                              acgacCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACGCCCtgg    >  3:232308/1‑135 (MQ=255)
                                                                                                                                gacCGCCGCCTGCTGGGCGCCCTGCCCAGGCCCTCGCGCCTGGCCAAGGCCGACTATCAGGACCAGTGGATCCCCGAACCGGCGCGTCGGGCCGGCCTGCGGCGTGACACGCGCATGCGCCGGCACGCCCtggtg  <  1:120052/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
GGGTAGACCCGAATTACCGCAGCCTGGCGGTGGGGCGCATTCTGCTGGAAAGCCTGCAAGCCGCGCTGGCCCACAATCCCAAGGGCAAGCACCAGGGCAACGTGGCTCCATTGGGCCGCAGCATCGACGACCGCAGCCTGCTTGGCGCCCTGGACATGACCTTGCGCCTGGACAAGGCCGACTATCAGGAACAGTTGATCACCGAACAGGCGCGTCTGGCCGGCCTGCTGCGTGACAAGCGCATGCGCCGGCACGCCCTGGTG  >  ACB122/1947371‑1947633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑