Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,016,038 A→G 6.5% S5G (AGC→GGC)  tld → GDP‑6‑deoxy‑D‑talose 4‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,016,0380AG 6.5% 193.4 / 3.6 61S5G (AGC→GGC) tldGDP‑6‑deoxy‑D‑talose 4‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (32/25);  new base G (2/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

ATCGGCAACCCGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCGGCTGCGGACTACCGCCAGGT  >  ACB122/2015904‑2016168
                                                                                                                                      |                                                                                                                                  
aTCGGCAACCCGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTa                                                                                                                                    >  3:59177/1‑135 (MQ=255)
        cccGGCCAAAGCCAAGGCCGTTCTGGGGTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  5:10515/135‑1 (MQ=255)
        cccGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  1:158992/135‑1 (MQ=255)
        cccGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  8:55424/135‑1 (MQ=255)
        cccGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  7:61467/135‑1 (MQ=255)
        cccGGCCAAAGCCAAGGACGTTCTGGGGTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATTGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  1:225285/135‑1 (MQ=255)
        cccGGCCAAAGCCAAGGAAGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  2:192129/135‑1 (MQ=255)
                  gCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTa                                                                                                                  <  6:134757/135‑1 (MQ=255)
                  gCCAAGGACGTTCTGGGGTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTa                                                                                                                  <  6:38798/135‑1 (MQ=255)
                       ggACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGgt                                                                                                             <  8:237752/135‑1 (MQ=255)
                         aCGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGgtgt                                                                                                           <  8:258314/135‑1 (MQ=255)
                         aCGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGgtgt                                                                                                           <  7:460845/135‑1 (MQ=255)
                         aCGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGgtgt                                                                                                           >  2:39016/1‑135 (MQ=255)
                               tGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTc                                                                                                         >  1:102677/1‑131 (MQ=255)
                               tGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTc                                                                                                         <  2:102677/131‑1 (MQ=255)
                               tGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACgg                                                                                                     >  2:166035/1‑135 (MQ=255)
                                gggTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACggg                                                                                                    >  7:128634/1‑135 (MQ=255)
                                gggTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACggg                                                                                                    >  3:118547/1‑135 (MQ=255)
                                      ggAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAc                                                                                               <  7:352685/134‑1 (MQ=255)
                                       gAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAcc                                                                                              <  5:173077/134‑1 (MQ=255)
                                         gCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGc                                                                                           >  1:88310/1‑135 (MQ=255)
                                         gCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGc                                                                                           >  8:389960/1‑135 (MQ=255)
                                                aCAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACa                                                                                    >  5:250547/1‑135 (MQ=255)
                                                       ttGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGc                                                                             >  3:33003/1‑135 (MQ=255)
                                                       ttGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGc                                                                             >  2:389438/1‑135 (MQ=255)
                                                       ttGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGc                                                                             >  7:227046/1‑135 (MQ=255)
                                                       ttGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGc                                                                             >  4:287076/1‑135 (MQ=255)
                                                       ttGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACAGGCCGCTACATCGCAGc                                                                             >  2:67721/1‑135 (MQ=255)
                                                            gaaCTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGt                                                                        >  7:218261/1‑135 (MQ=255)
                                                                  tgCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCggg                                                                  >  1:370603/1‑135 (MQ=255)
                                                                  tgCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCggg                                                                  >  8:1312/1‑135 (MQ=255)
                                                                  tgCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGCTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCggg                                                                  >  5:237325/1‑135 (MQ=255)
                                                                   gCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCgg                                                                   >  2:52537/1‑133 (MQ=255)
                                                                   gCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGc                                                                 >  3:401791/1‑135 (MQ=255)
                                                                   gCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGc                                                                 <  3:287076/135‑1 (MQ=255)
                                                                   gCCGTATGATGGGCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGc                                                                 <  5:441378/135‑1 (MQ=255)
                                                                     cGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCcg                                                               >  4:126597/1‑135 (MQ=255)
                                                                      gTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGATCACCGGCCGCTACATGGCATCCGAGTTGCGGGCcgg                                                              >  8:152292/1‑135 (MQ=255)
                                                                          gatgGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTCATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCggcggc                                                          >  8:201739/1‑135 (MQ=255)
                                                                            tgGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCt                                                         <  4:118547/134‑1 (MQ=255)
                                                                             ggTCGAAGCCGACCTGCGTCGTAACGAAAAATGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGGGGTCACGGGTTCACCGGCCGCTACCTGGCAGCCGAGTTGCGGGCCGGCGGCTAt                                                       <  6:195673/135‑1 (MQ=255)
                                                                                 gAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAACGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCTCGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAg                                                   >  7:297776/1‑135 (MQ=255)
                                                                                 gAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAg                                                   >  8:336575/1‑135 (MQ=255)
                                                                                     ccGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTGGCAAgcgc                                                                                                                            <  4:79857/58‑1 (MQ=255)
                                                                                     ccGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTGGCAAgcgc                                                                                                                            >  3:86245/1‑58 (MQ=255)
                                                                                     ccGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTGGCAAgcgc                                                                                                                            >  3:79857/1‑58 (MQ=255)
                                                                                     ccGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTGGCAAgcgc                                                                                                                            <  4:86245/58‑1 (MQ=255)
                                                                                      cGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGc                                                                 >  4:79247/1‑116 (MQ=255)
                                                                                      cGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGc                                                                 <  3:79247/116‑1 (MQ=255)
                                                                                       gACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            >  7:368791/1‑56 (MQ=255)
                                                                                       gACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAgcgc                                                                                                                            <  8:368791/56‑1 (MQ=255)
                                                                                         ccTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGt                                           >  5:349962/1‑135 (MQ=255)
                                                                                            gCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACa                                        >  7:116285/1‑135 (MQ=255)
                                                                                                cgtAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAcc                                                                                              <  2:6154/77‑1 (MQ=255)
                                                                                                cgtAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAcc                                                                                              >  1:6154/1‑77 (MQ=255)
                                                                                                               tgctcGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCgg                     <  8:411074/133‑1 (MQ=255)
                                                                                                               gtctcGTTCTGATGCAACAGGCTAGCAAGCGCGCGCGGATTACAGGTGTGCACGGGTGCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCgg                     <  1:9460/134‑1 (MQ=255)
                                                                                                                   cGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCCGCCAGCCCTTGCCGGCTGc                 >  1:94455/1‑135 (MQ=255)
                                                                                                                    gTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAcc                                                                                              <  1:168363/57‑1 (MQ=255)
                                                                                                                    gTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCAcc                                                                                              >  2:168363/1‑57 (MQ=255)
                                                                                                                              aaaaGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCGGCTGCGGACTACCGc       <  4:191089/134‑1 (MQ=255)
                                                                                                                                  ggCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCGGCTGCGGACTACCGCCAGGt  >  7:93023/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                  
ATCGGCAACCCGGCCAAAGCCAAGGACGTTCTGGGTTGGGAGCCTAAGACAAACCTTGAAGAACTGTGCCGTATGATGGTCGAAGCCGACCTGCGTCGTAACGAAAAAGGGTTCTCGTTCTGATGCAAAAGGCTAGCAAGCGCGCGCTGATTACAGGTGTTCACGGGTTCACCGGCCGCTACATGGCAGCCGAGTTGCGGGCCGGCGGCTATGAAGTCTTCGGTACAGGCAGCCAGCCGTTGCCGGCTGCGGACTACCGCCAGGT  >  ACB122/2015904‑2016168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑