| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,085,482 | C→G | 24.4% | intergenic (+71/+20) | deaD → / ← ygiD | ATP‑dependent RNA helicase DeaD/4,5‑DOPA dioxygenase extradiol |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,085,482 | 0 | C | G | 24.4% | 34.1 / 3.1 | 35 | intergenic (+71/+20) | deaD/ygiD | ATP‑dependent RNA helicase DeaD/4,5‑DOPA dioxygenase extradiol |
| Reads supporting (aligned to +/- strand): ref base C (9/17); new base G (5/4); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAAT > ACB122/2085354‑2085608 | cAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTAGCGGGCGTGccc > 5:105577/1‑135 (MQ=255) gACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCGGGCGTAACGAAAAAATCCCCGGCTTAGGGCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTGGCCC‑GCGa < 4:382735/135‑1 (MQ=255) gACACTCGCGGGGCGGGCAAGCCGCTGAAGCTGCGTCGCTACCGCGCGTATTGATCCTGGCGAAACCAAAAAATCCCCGGCTTAGGGCGGGTATTTTTTTTACCTGACCCGCCCCTTCCGCGGGCTTCCCC‑GCGa < 6:380997/135‑1 (MQ=255) acacTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCc‑gcgc > 8:24029/1‑133 (MQ=255) acacTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGaa > 6:123110/1‑135 (MQ=255) gcgTGGCTGGCAAGCAGCTGAAGATGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTAACGGCCATTTAGCCGGCTTTCCC‑GCGAAGCGGcc > 6:60893/1‑135 (MQ=255) cgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGGGTGCCC‑GCGAAGAGGccc > 7:163922/1‑135 (MQ=255) cgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGGCC‑GCGCAGAGGgcc > 1:308195/1‑135 (MQ=255) gggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACCAAAAAATCCCCGGCTTAGGGCGGGTATTTTTTTTACCTGTACCGGCCCTTGCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑Gt < 1:296081/134‑1 (MQ=255) gcAAGCAGCTGAAGCTGGGTCGCTACCGCGAGTATTGAGCGTGGCTTAACTAAAAAATCCCCGGGTTAGGTCGGGTTTTTTTTTTACCTGTACCGGCCCTTTCGCGGGGTTGCCC‑GGGAAGAGGCCCTTCA‑GTCg < 8:62443/135‑1 (MQ=255) gcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTAGCGGGGGTGGCC‑GCGCAGGGGCCCGTGA‑Gtg > 6:360386/1‑133 (MQ=255) agcTGAAGCTGCGGCGCTACCGCGAGTATTTATCCGGGCGTAACTAAAAAATCCCGGGCTTCGGGCGGGTATTTTTTTTACCTGTACCGCCCCTTTCGCGGGCTGGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGa < 5:123110/135‑1 (MQ=255) aGCTGCGTGGCTTCCGCGAGTATTGAGCCTGGCTTAACGAAAAAATCCCCTGCTTAGGGCGGGTTTTTTTATTACCTGACCCGGCCCTTGCCGGGGGGCCCCG‑GGGAAGAGGCCCTTCA‑GTCGAACCGATAGAt < 6:214984/134‑1 (MQ=255) aGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTACCGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTCCCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGAt < 7:267291/134‑1 (MQ=255) gCTACCGCGACGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAACAGGCCCATCA‑GTCGAACCGAAAGATGTCGATcccc > 8:346742/1‑135 (MQ=39) cgcgAGGATTGACCCGGGCGTACCGAAAAAATCCCCGGCTTAGGGGGGGTTTTTTTTTTACCTGAACGGCCCCTTGCGCGGGCTTCCCG‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAgcgc < 3:188672/135‑1 (MQ=255) gcgAGTATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAgcgcg < 7:386759/135‑1 (MQ=255) tttGCTCCTGGCGAACCCAAAAAAGCCCGGGCTTAGGGCGGTTATTTTTTTAACGTGAACCGGCCGTTGCGGGGGCTGCCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAgg < 6:352195/134‑1 (MQ=255) cTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGCGGCCCGTCC‑GTCGAACCGATAGATGTCCCTCCCCAGCCCGCCCCGGGTAAAcc > 6:133974/1‑135 (MQ=39) gggCGTACCGAAAAAAGCCCGGGCTTAGGGGGGGGATTTTTTTTACCTGTCCCGGCCCTTTCGCGGGCTTGCCC‑GGGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAAccc < 8:45164/134‑1 (MQ=255) gTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTGGCGGGCTTGGCC‑CCGGACAGGCCCCTCATGTGCAACGGATTG‑TGTCCAAGCCCCGCGGGCCCACGGGAAAacccg > 3:21411/1‑132 (MQ=255) gTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTACATCCCCCGCGCGCCCAGGGTAAACCCCTGa > 4:232194/1‑135 (MQ=255) tAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGGGTGGCC‑GGGGAGAGGCCCGTCA‑GTCGCGCCGCTTGCTGTGCCTGCCCCGCGCGCCCCGGGGTAACCCCGGAt > 1:244252/1‑135 (MQ=255) tAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTAGCGGGGGTTCCC‑CCGTATCGGCCCGTCA‑GTGGGACCCAGACAATTCCCGACCCAGCGCGCCCCGGGGATAccccggct > 8:20327/1‑131 (MQ=255) tAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTAGCGGGGGTGGCC‑GCGAAGCGGCCCGTCA‑GTCGACGCGATTGGTGTCCATCCCCCGCGCGCCCACGGTAAACCCCTGag > 2:259446/1‑134 (MQ=255) ccaaaaaaaCCCCCGGCTTAGGTGGGGTTTTTTTTTTACCTCTACCGCCCGTTTCGCGGGGTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATg < 5:69667/131‑1 (MQ=255) cGGGTATTTTTTTTACCTGTACGGGCCATTTCGGGGGCTGGCGC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGc < 5:226146/135‑1 (MQ=255) cGGGTATTTTTTTTACCGGTACGGCCCGTTTGGGGGGGTTCCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGc < 1:61117/135‑1 (MQ=255) cGGGGATTTTTTTTACCTGAACCGGCCCTGGCGCGGGCTTCCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGc < 7:282256/135‑1 (MQ=255) gggTTTTTTTTTACCTGTACCGCCCCTTCCGGGGGCTTCCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGccc < 6:68914/135‑1 (MQ=255) tGTACCGGCCCTTGCGCGGGCGTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACa < 6:454280/135‑1 (MQ=255) gCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGgcag < 4:153962/135‑1 (MQ=255) gCCCTTCCCCGGGCGGGCCG‑GCCAAGAGGCCCTCCA‑GACGAACCGATAGATGTCCAACCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGgcag < 1:259446/135‑1 (MQ=255) gctgggCCTGCCCGGCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAAt < 5:60893/131‑1 (MQ=255) cgcgGGCTGGCCC‑GCTAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAAt < 3:167090/133‑1 (MQ=255) | CAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCC‑GCGAAGAGGCCCTTCA‑GTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAAT > ACB122/2085354‑2085608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |