Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	194,699	G→C	5.8%	V3186V (GTG→GTC)	CJ019_00185 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	194,699	0	G	C	5.8%	179.2 / 3.3	64	V3186V (GTG→GTC)	CJ019_00185	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/36);  new base C (2/2);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCA  >  ACB122/194569‑194826                                                                                                                                   |                                                                                                                                tGGGCATCACCGCGGCGGTGGACGCCACTGGTGCTGCGTGCGAGACCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCCTCGCCGAAGTCGGGGCGAAGCTGACCGCGACCCCTTCGGTGACCg                                                                                                                             <  2:334945/135‑1 (MQ=18)  gggCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGAcc                                                                                                                              >  2:82152/1‑133 (MQ=255)  gggCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGAcc                                                                                                                              >  1:443760/1‑133 (MQ=255)     cATCCACTCGGCGGTGGACGCCAGTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGCCAACGCTTCGGTTCAAGTGACCGACACCCTCGACTAAGGCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAgg                                                                                                                         <  2:294142/135‑1 (MQ=18)     cATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGCCCCCTTCGGTGACCGAAgg                                                                                                                         >  5:252010/1‑135 (MQ=255)     cATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAgg                                                                                                                         >  8:267397/1‑135 (MQ=255)       tCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCTGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcg                                                                                                                       >  7:107055/1‑135 (MQ=255)       tCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcg                                                                                                                       >  1:109779/1‑135 (MQ=255)       ccaccTCGGCGGGGGACGCCACGGGTGCTGCGTTCGAGACCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGGGACCGACACCCTCGCCGACGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcg                                                                                                                       <  3:136891/131‑1 (MQ=17)        caccggggcggTGCACGCCACTGGGGCTGCGTTCGAGAACCTGCACCGGGGCGGCAACGCGTCGGGTCAAGGCCCCGACACCCGCGCCGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcgg                                                                                                                      <  7:124863/129‑1 (MQ=17)         accTCGGCGGGGGCCGCCACGGGGGCGGCGTGCGAGAACCTGCAACTGGGCGGCAACGCTGCGGTTCAAGTGACCGACACCCTCGACGAAGTCGGGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  6:244852/133‑1 (MQ=17)         aaCTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCGTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  2:281215/135‑1 (MQ=255)         aaCTCGGCGGTGCACCCCACTGGGGGTGCGTTCGAGAACCTGCAACTGGGCGGAAACGCTTCGGTGCAAGTCACCGACACCCTCGACGAAGTCGTGGCGACGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  6:395242/135‑1 (MQ=17)          agtCGGCGGTGGACGCCACTGGTGCGTCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCTAAGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  1:3976/132‑1 (MQ=255)          aCTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  4:234002/134‑1 (MQ=255)          aCTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGgcggcg                                                                                                                    <  6:191639/135‑1 (MQ=255)          aCTCGCGGGGGGACGCCACTGGTGCTGCGTTCGAGACCGTGCAACTGGCCGGCAACGCTTCGGTTCAAGGGACCGACACCCTGCACGATGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcggc                                                                                                                     <  6:245171/134‑1 (MQ=17)            tCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGCCACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcgg                                                                                                                      >  5:64836/1‑131 (MQ=255)            tCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcgg                                                                                                                      >  6:424517/1‑131 (MQ=255)            tCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAggcgg                                                                                                                      >  1:312646/1‑131 (MQ=255)                 ggtggtCGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAAGGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCAc                                                                                                             <  6:428731/135‑1 (MQ=255)                 gggggccGCCAGTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTGCGGTTCAAGTGACCGACACCCTCGACGAAGTCGGGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCAc                                                                                                             <  8:86205/129‑1 (MQ=17)                 gggggccGCCACGGGGGCTGCTTTCGAGAACCGGCAAGTGGGCGGCAAGGCTTCGGTTCAAGGGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCAc                                                                                                             <  8:314383/129‑1 (MQ=14)                 gggggACGCCACGGGTGCGGCGTTCGAGACCCTGCAACGGGGCGGCAACGCTGCGGTTCAAGTGACCGACACCCTCGACGACGTCGGGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCAc                                                                                                             <  3:372693/132‑1 (MQ=17)                 ggTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCAc                                                                                                             <  6:217891/135‑1 (MQ=255)                      aCGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTac                                                                                                         <  4:415968/134‑1 (MQ=255)                        gCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACCCCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTacac                                                                                                       >  1:367768/1‑134 (MQ=255)                           aCTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCCGTGCCCGAAGGCGGCGAGATCCCCTACACCATc                                                                                                   >  1:199409/1‑135 (MQ=255)                              ggtggtGCGTGCGAGACCCTGCAACTGGGGGGCAAGGGTGCGGTTCAAGTCACCGACACCCTCGCCGAAGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACg                                                                                                <  2:18527/135‑1 (MQ=14)                                 gctggGTTCGAGAACGTGCAAGGGGGCGGCAAGGTTTGGGTGAATGTGACCGACCCCCGCGACTAGGTGGGGGGGAAGCTGACCGCGACCCCTTGGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCtc                                                                                             <  4:279162/135‑2 (MQ=2)                                  ctgcGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGACGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAg                                                                                                                          <  4:132730/105‑1 (MQ=255)                                  ctgcGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGa                                                                                            >  3:96971/1‑135 (MQ=255)                                  ctgcGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAg                                                                                                                          >  3:132730/1‑105 (MQ=255)                                       ttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaa                                                                                        >  3:237129/1‑134 (MQ=255)                                        tCGAGAACCTGCATCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaac                                                                                       >  6:317107/1‑134 (MQ=255)                                        tCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaaca                                                                                      <  1:82152/135‑1 (MQ=255)                                          gagaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaacaa                                                                                     <  4:452261/134‑1 (MQ=255)                                          gagaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaac                                                                                       >  2:166755/1‑132 (MQ=255)                                          gagaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaac                                                                                       >  3:156406/1‑132 (MQ=255)                                                    aaCTGGCGGGCAAGGCTTCGGTTCACGTCACCGACACCCTCCCCGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCt                                                                            <  3:311568/133‑1 (MQ=14)                                                            ggcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCaa                                                                   >  8:337617/1‑134 (MQ=255)                                                                aggCTTCGGTTCACGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAAcc                                                              <  3:126612/133‑1 (MQ=255)                                                                acgcggcGGTTAAAGGCACCCACCCCCGCGACGAGGGGGGGGCGAAGATGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAAcc                                                              <  5:5515/129‑1 (MQ=11)                                                                aCGCTTCGGTTCATGTGCCCGACACCCTCCACGATGTCGTGGCTACGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAAcc                                                              <  3:254108/135‑1 (MQ=14)                                                                  gCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCAc                                                            <  4:139913/135‑1 (MQ=255)                                                                      cGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAg                                                                                                                          >  7:14736/1‑69 (MQ=25)                                                                               tGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACt                                               >  7:235760/1‑135 (MQ=255)                                                                                 aCCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACtt                                              >  5:336659/1‑134 (MQ=255)                                                                                    gACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAg                                          <  7:375923/135‑1 (MQ=255)                                                                                       acCCTCGACTAGGTCGTGGCGAAGGGTCCCGCGACCACTTCAGTCTCAGAAGGCGGCGAGATCACCTACACCATAACGCTCACCAACAAAGACTGTCTGCCGATCAAAAACCACTCGGAGCTGTACTTCAAGCTg                                       <  4:289691/135‑1 (MQ=11)                                                                                       acCCTCGACGAAGGCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTg                                       <  7:365595/135‑1 (MQ=255)                                                                                         ccTCGCCGAAGGGGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGAc                                     <  6:228724/135‑1 (MQ=255)                                                                                         ccTCGCCGAAGGCGGGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGAc                                     <  8:347421/135‑1 (MQ=255)                                                                                              acgaAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGa                                  <  2:367768/133‑1 (MQ=255)                                                                                              acgaAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATg                                >  8:87922/1‑135 (MQ=255)                                                                                                     cGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCactac                         >  2:283213/1‑135 (MQ=255)                                                                                                          cGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACTCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCACGCTGACCGATGGCGCTACTgtcg                    >  5:87214/1‑135 (MQ=255)                                                                                                          cGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTgtcg                    >  6:189235/1‑135 (MQ=255)                                                                                                             aGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGtcgtcg                 <  7:267397/135‑1 (MQ=255)                                                                                                               cTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTg               <  6:64836/135‑1 (MQ=40)                                                                                                               cTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTg               <  1:283213/135‑1 (MQ=40)                                                                                                                     gcgACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCCCTCGGAGCTGTACTTCAAGCTGCCCGATGGCCCTACTGTCGTCGTGGCAGc          >  3:29578/1‑134 (MQ=255)                                                                                                                            cTTCGGGGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACACAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGca  <  2:117027/135‑1 (MQ=18)                                                                                                                                 gTGACCTAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTc                                             <  3:58222/87‑1 (MQ=18)                                                                                                                                   |                                                                                                                                TGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCA  >  ACB122/194569‑194826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑