Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,103,046 C→T 6.5% A1433T (GCC→ACC)  CJ019_02004 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,103,0460CT 6.5% 199.3 / 3.4 61A1433T (GCC→ACC) CJ019_02004hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/26);  new base T (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

ACCGGCGGCTGCCCAGGCAGCCGCGATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGCCGTAGTAGTT  >  ACB122/2102915‑2103175
                                                                                                                                   |                                                                                                                                 
aCCGGCGGCTGCCCCCGCCGCCGCGCTTGCCGGCGCCGCGACGCCCCCAGTCACCCCCCCGGCGGCCACGGCGGCCACTGCGGCGAAGAGTATGCCCCCGCCGAACCCCGGCCCGCCGCTCGCCCCCAACGCCtt                                                                                                                                >  2:296582/1‑135 (MQ=255)
                gCAGCCGCGATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTg                                                                                                                <  1:296582/135‑1 (MQ=255)
                    ccGCGATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGc                                                                                                            <  5:64477/135‑1 (MQ=255)
                        gATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAg                                                                                                             <  2:362787/130‑1 (MQ=255)
                        gATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAg                                                                                                             >  1:362787/1‑130 (MQ=255)
                        gATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTAt                                                                                                         >  5:329320/1‑134 (MQ=255)
                        gATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTATAGCTAt                                                                                                         >  4:387769/1‑134 (MQ=255)
                        gATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGACACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTAt                                                                                                         >  6:413976/1‑134 (MQ=255)
                                 cgcAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTg                                                                                                >  2:60558/1‑134 (MQ=255)
                                    aGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAg                                                                                            >  6:3604/1‑135 (MQ=255)
                                     gCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGc                                                                                           <  3:297873/135‑1 (MQ=255)
                                         cGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGTTGCTGTAGCTATTCAGGTCTGCCAGCGATc                                                                                       <  3:12901/135‑1 (MQ=255)
                                            cACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGc                                                                                    >  4:333686/1‑135 (MQ=255)
                                              ccTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGc                                                                                                                  >  6:282317/1‑103 (MQ=255)
                                              ccTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGc                                                                                                                  <  5:282317/103‑1 (MQ=255)
                                               cTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTATAGCTATTCAGGTCTGCCAGCGATCGGCttt                                                                                 <  3:387769/135‑1 (MQ=255)
                                                 gTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGc                                                                               >  4:182422/1‑135 (MQ=255)
                                                   caccacGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGccc                                                                             >  2:9573/1‑135 (MQ=255)
                                                   caccacGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGccc                                                                             >  3:177745/1‑135 (MQ=255)
                                                   caccacGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGccc                                                                             <  1:60558/135‑1 (MQ=255)
                                                       acgacgGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCt                                                                          >  5:133766/1‑134 (MQ=255)
                                                          actgcggcCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAg                                                                      <  1:36597/132‑1 (MQ=255)
                                                           cggcggCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGc                                                                     >  1:248267/1‑135 (MQ=255)
                                                                     ggAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTa                                                           <  2:214302/135‑1 (MQ=255)
                                                                                  gcgAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCggtcggg                                              >  3:64888/1‑135 (MQ=255)
                                                                                     aaGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGc                                                                                                                  >  3:279059/1‑64 (MQ=255)
                                                                                     aaGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGc                                                                                                                  <  4:279059/64‑1 (MQ=255)
                                                                                      agagTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTcgc                                          <  5:413976/135‑1 (MQ=255)
                                                                                             gCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGGCGCggcgggg                                   >  1:218237/1‑130 (MQ=255)
                                                                                                 aGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATc                               <  4:144965/135‑1 (MQ=255)
                                                                                                     cTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCt                                                                                   >  3:387079/1‑79 (MQ=255)
                                                                                                     cTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCt                                                                                   <  4:387079/79‑1 (MQ=255)
                                                                                                     cTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGGAGGTTATCGGGt                           >  2:197490/1‑135 (MQ=255)
                                                                                                     cTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGt                           >  6:148342/1‑135 (MQ=255)
                                                                                                     cTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGt                           >  7:4626/1‑135 (MQ=255)
                                                                                                      tATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGtt                          <  6:187357/135‑1 (MQ=255)
                                                                                                      tATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGtt                          <  8:4626/135‑1 (MQ=255)
                                                                                                      tATCCCCGTCCAGCCGATCGAACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATGCAGGTCTGCCAGCGATCGGCTTTGCCCGCTTTAGCCGATGCTGTAATGCCCGGTAGGGTCGCGGAAGGTTATCggggt                          <  3:126419/135‑3 (MQ=255)
                                                                                                      tATCCCCGTCCAGCCGAGCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATTCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGtt                          <  3:454246/135‑1 (MQ=255)
                                                                                                           ccGTCCAGCCGCTCGCACCTAAGGCCTGGCCCCGCCAGATGCGGTAGCGATTCAGGTCTGCCAGCGATCGCCTTTGCCCGCTGTAGCGGATGCTGTAATGCCCGGTCGCGTCGCGGCAGGGTATGCGGATGCCtt                     >  2:441346/1‑135 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCt                                                                                   >  7:42151/1‑73 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCt                                                                                   <  8:42151/73‑1 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGTAGGTTATCGGGTTGCCtt                     >  7:24806/1‑135 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGTAGGTTATCGGGGTGCCtt                     >  3:399732/1‑135 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGGTATCGGGTTggcgt                     >  4:44338/1‑131 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGGTATCGGGTTGCCtt                     >  3:408338/1‑135 (MQ=255)
                                                                                                           ccGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGGTATCGGGTTGCCtt                     >  8:200574/1‑135 (MQ=255)
                                                                                                            cGTCCAGCCGATCGAACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTg                    <  2:378587/135‑1 (MQ=255)
                                                                                                                cAGCCGATCGCACCTAAGGTCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGc                                                                                           <  1:189513/60‑1 (MQ=255)
                                                                                                                cAGCCGATCGCACCTAAGGTCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGc                                                                                           >  2:189513/1‑60 (MQ=255)
                                                                                                                cAGCCGATCGCACCTAAGGTCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGc                                                                                           >  2:183698/1‑60 (MQ=255)
                                                                                                                cAGCCGATCGCACCTAAGGTCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGc                                                                                           <  1:183698/60‑1 (MQ=255)
                                                                                                                cAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCaa                <  7:142557/135‑1 (MQ=255)
                                                                                                                 aGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTCTCGGGTTGCCTTGGCAAt               <  1:9573/135‑1 (MQ=255)
                                                                                                                 aGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGGTATCGGGTTGCCTTGGCa                 >  4:143787/1‑133 (MQ=255)
                                                                                                                 aGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTGTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAAt               <  3:173305/135‑1 (MQ=255)
                                                                                                                     gATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGcc           <  5:148342/135‑1 (MQ=255)
                                                                                                                      aTCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGGCTTGGCAATAGCCg          >  8:130718/1‑135 (MQ=255)
                                                                                                                      aTCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGCCg          >  3:417525/1‑135 (MQ=255)
                                                                                                                      aTCGCACCTAAGGCCTAGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGCCg          <  2:192412/135‑1 (MQ=255)
                                                                                                                              tAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGCCGTAGTAGtt  <  7:200574/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
ACCGGCGGCTGCCCAGGCAGCCGCGATTGCCGGCGCAGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATGCTGTAGCTATTCAGGTCTGCCAGCGATCGGCTTTGCCCGCTGTAGCCGATGCTGTAATGCCCGGTCGGGTCGCGGAAGGTTATCGGGTTGCCTTGGCAATAGCCGTAGTAGTT  >  ACB122/2102915‑2103175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑