Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,131,359 T→G 27.5% R135R (CGT→CGG)  yadG → putative ABC transporter ATP‑binding proteinYadG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,131,3590TG27.5% 51.9 / 3.3 49R135R (CGT→CGG) yadGputative ABC transporter ATP‑binding proteinYadG
Reads supporting (aligned to +/- strand):  ref base T (15/19);  new base G (13/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTCGAAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAG  >  ACB122/2131225‑2131484
                                                                                                                                      |                                                                                                                                 
cAGTTCGAAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTTGGCCTGTGGGACAAACGTGATGTGCAGTCGcgt                                                                                                                                   <  5:218657/135‑1 (MQ=255)
      gAAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTg                                                                                                                             >  5:455093/1‑135 (MQ=255)
        aaaGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑c                                                                                                                          <  4:306755/135‑1 (MQ=255)
                   aCATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTTT‑CCGGCGGCAt                                                                                                                 >  1:87298/1‑133 (MQ=255)
                     aTCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAg                                                                                                             <  6:432440/135‑1 (MQ=255)
                          cgtGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGGTGTGGT‑CCGGCCGCCAGCAgcggcg                                                                                                        >  8:358550/1‑135 (MQ=255)
                           gtGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGT‑GCGGCCGCATGAAGCCgcgc                                                                                                       >  6:64149/1‑135 (MQ=255)
                                ccAGGACTGGTACTACGGAATCACGCCCAAGCTGTCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGATGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCtga‑t                                                                                                 <  1:219181/135‑1 (MQ=255)
                                      cGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGT‑CCGGCGGCATGCAGCGGCGCCGGG‑TGCTTGc                                                                                           >  3:181498/1‑135 (MQ=255)
                                           actacGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTGGTGGT‑CCGGCGGCCTGAATCGGCGCGGGA‑TGATATCCcgc                                                                                       >  3:209395/1‑134 (MQ=255)
                                            ctacGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCgcgc                                                                                     >  7:73464/1‑135 (MQ=255)
                                             tacGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGGACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCgcgc                                                                                     <  6:379539/134‑1 (MQ=255)
                                                 gCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGT‑GCGGGGGGATGTAGAGGCGCCTGG‑TGATTGACCGCGCAGTGa                                                                                >  7:267482/1‑135 (MQ=255)
                                                       cGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTGGTTGT‑GCGGCGGCCTGAAGCGGCGCCGGG‑TGTTTTCCCGCGCATTGGTCCACg                                                                          >  3:406182/1‑135 (MQ=255)
                                                       cGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACg                                                                          <  3:329555/135‑1 (MQ=255)
                                                       cGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGGTGT‑CCGGGGGCGTGAAGCGGCGCCGGA‑TGCTTGCCCGCGCAGTGCTGCAc                                                                           >  5:114865/1‑134 (MQ=255)
                                                           cAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAAcc                                                                      <  1:77660/135‑1 (MQ=255)
                                                           cAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAAcc                                                                      <  5:152969/135‑1 (MQ=255)
                                                               cTGGCCCAGGAGCGCGCCGAGCAGTACCTGACTCAGCGGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGGTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCgcgc                                                                  <  6:111187/135‑1 (MQ=255)
                                                                tGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGcc                                                                 <  2:61951/135‑1 (MQ=255)
                                                                   ccAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCct                                                                <  6:455093/133‑1 (MQ=255)
                                                                      aGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGa                                                           >  8:109408/1‑135 (MQ=255)
                                                                      aGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGT‑GCGGCGGCATGAAGCGGCGCCGGA‑TGATTGCCCGCGCATTGATCCACGCACCGCGCcggcggc                                                           >  4:403450/1‑132 (MQ=255)
                                                                               ccGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCCTTGATCCACGAACCGCGCCGGCTGATCCTGG‑Ac                                                  >  3:429878/1‑134 (MQ=255)
                                                                               ccGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGT‑CCGGGGGCATGTAGCGGCGCCGGT‑TGATTGCCCGCGCATTGACCCCCGAACCGCGCCGGCTGATCCTgggc                                                   >  4:90142/1‑132 (MQ=255)
                                                                                     aGTACCTGACTCAGCTGGGCCGGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCa                                          <  4:432049/135‑1 (MQ=255)
                                                                                       tACCTCACTCAGCTGGGCCTGGGGGACAATCGTGATGTGCAGTCGCGTGCGTTGT‑CCGGCGGCATCAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCAcc                                        <  6:123033/135‑1 (MQ=255)
                                                                                         ccTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGGCGCGTTCGTTGT‑CCGTCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCAccgc                                      <  5:64149/135‑1 (MQ=255)
                                                                                          cggccgcAGCGGGGCCTGTGGGACAACCGGGATGGGCAGTCGCGTGCGGGGG‑CCGGCGGCATCAAGGGGCGCCTGA‑GGATTGCCCGCGCATTGCTCCACGAACCGCGCCTGCTGATCCTGG‑ACGAC‑CCCAccgcc                                     <  2:130018/129‑1 (MQ=255)
                                                                                          cTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGGTGTAACGCGGGCAT‑AAGCCGCGGCCGA‑ATATTGGCCGCGCCATGATCCACGAACCCCCCCCGGCGGTGCTGG‑CCGGA‑CCCCccgcc                                     >  4:260919/1‑135 (MQ=255)
                                                                                           tGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTTCCCGCGCATTGATCCACGCACCCCGCCGGCGGAGCCTGG‑GCGAA‑CCCAccgccg                                    >  7:31009/1‑135 (MQ=255)
                                                                                              cTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGGCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCgg                                   <  5:167414/133‑1 (MQ=255)
                                                                                              cTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGGCGCGTTCGTTGG‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCgg                                   <  2:103194/133‑1 (MQ=255)
                                                                                               tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTTT‑CCGGCGGCATGAACCGGCGCCTGA‑TGATTTCCCGCGCATTTATCAAAGAACCCCCCCTGCTGATACTTG‑ACGAACCCCACCCCCgggt                                 >  8:58243/1‑134 (MQ=255)
                                                                                               tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGGTGT‑CCCGCCGCATGAAGCCGGGCCGGA‑TGATTTCCCCCGCATTTATCAACAAACCCCGCCGGCGGATCCAGG‑AAGAA‑ACCACCCCCGGGGt                                >  4:304176/1‑135 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTGGGTGT‑ACGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCCAGAACCGCGCCGGCGGATCCTGG‑CCGAA‑CCCACCCCCCGGGTGGat                            >  8:158271/1‑134 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTGGGTGT‑ACGGCGGCATGAAGCGGCGCCGGA‑TGAATGCCCGCGCATTGATACACGAACCGCGCCGGCTGCACCTGG‑CCGAA‑CCCCCCGCCGGGGTGGtta                           >  6:14105/1‑135 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TTATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGat                            >  8:382753/1‑134 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCCTTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGat                            >  8:58176/1‑134 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCGGA‑TGGTTGCCCGCGCATTTATCCACGAACCGCGCCAGCGGATCCTGG‑CCGCA‑CCCACCGCCGGGGTGGat                            >  2:242152/1‑134 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGT‑CCGGCGGCCTTAAGCGGCGCCGGA‑TGATTGCCCGCGCATTGTTACCCGAAACGCGCCGCCGGATGCTTG‑ACGCA‑CCCACCCCCGGGGTGGata                           >  4:353545/1‑135 (MQ=255)
                                                                                                    tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGT‑CCGGCGGCATGAAGCGGCGCCTGC‑TGATTGCCCGCGCATTGATCCACGCACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGat                            >  8:79635/1‑134 (MQ=255)
                                                                                                      ggCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTTT‑GCGGCGGGATGAACCCGCGCCGGATTAATTGCAC‑CGCATTGATAAAAGAACCGCCCCGGGGGGTCCAGGCACAAA‑CCC‑CCCCCGGGGGGGat                            >  6:390198/1‑132 (MQ=255)
                                                                                                       gCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGATATc                         <  8:311382/134‑1 (MQ=255)
                                                                                                           gtgGGACAAACGTGATGTGCAGTCGCGTTCGGTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCCTTGATCCCCGCACCGCGCCGGCTGATCCGGG‑ACGCA‑CCCACCCCCGGGGGGGATATAGAGCt                    >  1:58391/1‑135 (MQ=255)
                                                                                                                aCAAACGTGATGGGCAGGCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGATATCGAGCTGcgcc               <  2:368596/135‑1 (MQ=255)
                                                                                                                aCAAACGTGAGGGGCTGTCGCTGTCGTTGT‑CCGGCGGCTTGCCGCGGCGCCTGA‑TGAATCCCCGCGAATTGAGCCCCGAACCGCGCCTGCTGATCCTGG‑ACGAC‑CCCACCGCCGGGGTGGATATCGAGCTGcgcc               <  5:79464/135‑1 (MQ=255)
                                                                                                                     cGTGATGTGCAGTCGCGTTAGTTGT‑ACCGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATACACGCACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCCCCCGGGTGGATATCGAGCTGcgccgcgc           >  6:160618/1‑132 (MQ=255)
                                                                                                                     cGTGAGGGGCAGGCGCGTGCGTTGT‑CCGGCGGCAGGAAGCGGCGCCTGA‑GGATTGCCCGCGCATTGAGCCCCGACCCGCGCCTGCTGATCCTGG‑ACGAC‑CCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCg          <  8:208048/135‑1 (MQ=255)
                                                                                                                             gCAGTCGCGTTCGTTGG‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAg  <  3:403475/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
CAGTTCGAAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGT‑CCGGCGGCATGAAGCGGCGCCTGA‑TGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGG‑ACGAA‑CCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAG  >  ACB122/2131225‑2131484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑