Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,299,276	T→G	20.3%	N113T (AAC→ACC)	CJ019_02179 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,299,276	0	T	G	20.3%	85.8 / 2.5	55	N113T (AAC→ACC)	CJ019_02179	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/24);  new base G (10/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGTGGCCAGGTCGTCGGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTAACCAG  >  ACB122/2299147‑2299410                                                                                                                                  |                                                                                                                                       gtggtgGCCAGGTCGTCGGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTgggc                                                                                                                                   <  7:385691/135‑1 (MQ=255) gtggtgGCCAGGGCGGCGGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTgggc                                                                                                                                   <  7:342035/135‑1 (MQ=255) gtggggGCCAGGTCGTCGGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTgggc                                                                                                                                   <  5:301802/135‑1 (MQ=255)            ggcgtcgGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTc                                                                                                                        <  1:133232/133‑1 (MQ=255)               gtcgGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGc                                                                                                                     <  6:331778/135‑1 (MQ=255)                     cAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCAt                                                                                                               <  6:69172/135‑1 (MQ=255)                           cACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCgg                                                                                                         <  6:185437/135‑1 (MQ=255)                                gggTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGGCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCa                                                                                                    <  6:180813/135‑1 (MQ=255)                                     tgCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCg                                                                                               <  5:214642/135‑1 (MQ=255)                                        gcgGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGGTCCAGGCGGTTGGGGGGGCTGGGCGCGGCGCGCCTTTGCGGGGGCAGttggc                                                                                              >  1:115058/1‑130 (MQ=255)                                        gcgGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCGGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGCGCATTTGCGGGGGCAGTTCGa                                                                                              >  7:77083/1‑133 (MQ=255)                                        gcgGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGGGTTGAAGCAGGGCGAGGATCGTGCGCCGGGGCTGTTGCAGGCGGGTGGGCAGGCTTTGCGCGGCGCGGATTTGCGGGGTGAGGTCGGGc                                                                                            >  2:138117/1‑135 (MQ=255)                                        gcgGAGCCGGCATACACCACCAGCGCCGTCAGGCTGTCGCCGCGGTGTTCAAGCACGTCGAGGATCTTGCGCCCGGCCACTTCCACGCGGGTGGGCGGGCTGTCCACGGCGACCATTTGCGGGGTCAGTTCGacc                                                                                            >  2:148435/1‑133 (MQ=255)                                              ccGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTGCAGGCGGGTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATc                                                                                      >  2:31643/1‑135 (MQ=255)                                               cGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGGAGGTCGCGGATCTTGCGCCGGGGCTGGTGGCGGCGGGTGGGCGGGGGGTGCGCGGGCCGCAGTTGCGGGGTCAGTTCGCGCCGGGTCa                                                                                     >  1:280706/1‑135 (MQ=255)                                               cGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCGGTTCCCGGCGGGTGGGCGGGCTGTCCTCGGCGAGCCTTTGGGGGGGCAGGTCGCGCAGGATcc                                                                                     >  6:450198/1‑134 (MQ=255)                                               cGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCGGGTGCAGGCGGTTGGGCGGGCTGTCCGCGGCGCGCCTTTGCGGGGTCAGGTCGAGCAGGATCa                                                                                     >  8:88754/1‑135 (MQ=255)                                                         caccaGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCGGTTCCAGGCGGGTGGGGGGGCTGTCCTCGGCGCGCATTTGCGGGGTGCGTTCGCGCAGGGTCCCCCGCGGGTc                                                                           >  6:320761/1‑135 (MQ=255)                                                              gcgcCGTCTGGCTGTCGCGGGGGGGTTGAAGCAGGTCGAGGATCTTGCGCCGGGGCGGGGGCCGGGGGGTGGGGGGGGGGGGCGCGGGGGGCCATTGTGGGGGGGGTTGGGGCAGGGTCACCCCCGGGTCGGCCg                                                                      >  6:13984/1‑135 (MQ=255)                                                              gcgcCGTCTGGCTGTCGCGGCGGTGTTGAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTGCAGGCGGGTGGGGGGGGTGTGCTCGGCGCGCCTTTGGGGGGTCAGTTCGCGGAGGCTCACCAGCGGGTCGGCCg                                                                      >  4:38057/1‑135 (MQ=255)                                                                 ccGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGgc                                                                   <  3:61462/135‑1 (MQ=255)                                                                 ccGTCTGGCTGTCGCGGCGGTGGTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTGCAGGCGGGTGGGCGGGCTGTCCTCGGCGCGCAGTTGCGGGGGCCGTTCGAGCAGGCTCACCAGCGGGTCGGCCCGgc                                                                   >  1:55355/1‑135 (MQ=255)                                                                      tGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGGCTGTTGCCGGCGGGTGGGCGGGGGGTACGAGGCGCCCCTTTGGGGGGTGAGTTCGAGCAGGATCACCCGCGGGTAGGCCGGGCGCGGa                                                              >  6:69455/1‑135 (MQ=255)                                                                         cTGTCGCGGCGGTGTTCAAGCAGGTCGAGGAGCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACg                                                            <  6:438425/134‑1 (MQ=255)                                                                               cggcggTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGGCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTttct                                                     >  3:217187/1‑135 (MQ=255)                                                                                 gcggTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGGCAGTTCGAGCAGGATCACCAGCGGGTCGGCCCGGCGCTGACGGCTttcttc                                                   >  4:365181/1‑135 (MQ=255)                                                                                            gCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGcc                                        <  3:32140/135‑1 (MQ=255)                                                                                            gCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGcc                                        <  6:225951/135‑1 (MQ=255)                                                                                                 tCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTGCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTg                                   <  6:337554/135‑1 (MQ=255)                                                                                                   gAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTggg                                 >  8:439115/1‑135 (MQ=255)                                                                                                    aGGATCTTGCGCCGGGGCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCAATTGCGGGGGCAGTTCGAGCACGATCACCACCGGGTCCGCCCGGCGCGGGAGGCATTCTTACAGCCGCTGCCCGCgggga                                >  7:170093/1‑134 (MQ=255)                                                                                                      gATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGccc                              <  4:176656/135‑1 (MQ=255)                                                                                                      gATCTTGCGCCGGGCCTGTTCCAGGCGGTGGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGGCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGccc                              <  2:161649/135‑1 (MQ=255)                                                                                                      gATCTTGCGCCGGGCCTGTTCCAGGCGGGTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGccc                              <  2:209263/135‑1 (MQ=255)                                                                                                             cgcCGGGCCTGTTCCCGGCGGGTGGGCGGGCGGTCCTCGGCGAGCATTTGCGGGGGCAGTTCGAGCAGGCTCACCAGCGGGTCGGCCGGGCGCTGACGGCGTTCTTCCAGCCGCTGCCAGCTGGGCCCGagcagc                       <  5:4388/135‑1 (MQ=255)                                                                                                             cgcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTGCTCGGCGAGGATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCCGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGagccgc                       >  4:359808/1‑135 (MQ=255)                                                                                                             cgcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGCCGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGagcagc                       >  4:459993/1‑135 (MQ=255)                                                                                                             cgcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGagcagc                       >  8:225501/1‑135 (MQ=255)                                                                                                             cgcCGGGCCTGTTCCAGGCGGGTGGGCGGGCTGTCCTCGGCGAGCATTGGCGGGGGCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGNTTTCTTCCAGCCGCTGCCAGCTGGGCCCGagcagc                       <  5:10600/135‑1 (MQ=255)                                                                                                              gcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGTGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGGCGCTGCCAGCTGGGCCCGagcagc                       <  3:61548/134‑1 (MQ=255)                                                                                                              gcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGagcagc                       <  7:215696/134‑1 (MQ=255)                                                                                                              gcCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAgcg                      >  2:294932/1‑135 (MQ=255)                                                                                                                 gggCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCa                   >  1:328915/1‑135 (MQ=255)                                                                                                                  ggCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCATCCGCTGCCAGCTGGGCCCGAGCAGCGCCAg                  <  1:31643/135‑1 (MQ=255)                                                                                                                  ggCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAg                  <  3:343641/135‑1 (MQ=255)                                                                                                                     cTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGa                >  7:342034/1‑134 (MQ=255)                                                                                                                     cTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGa                >  8:58698/1‑134 (MQ=255)                                                                                                                         tCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCa             <  4:152673/133‑1 (MQ=255)                                                                                                                         tCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCa             <  8:342034/133‑1 (MQ=255)                                                                                                                          ccAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGCATCACCAGCGGGTCGGCCCGGCGCTGACGGCTTTCTTCCAGCCGCTGCCACCTGGGCCCCAGCCGCGCCAGGATCACCa          >  2:345966/1‑135 (MQ=255)                                                                                                                            aGGCGGTTGGGCGGGCTTTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGGTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGt        <  1:148435/135‑1 (MQ=255)                                                                                                                                ggTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGccc                              <  4:393295/109‑1 (MQ=255)                                                                                                                                ggTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGccc                              >  3:393295/1‑109 (MQ=255)                                                                                                                                ggTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTaa      >  1:359685/1‑133 (MQ=255)                                                                                                                                ggTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTaa      >  8:220266/1‑133 (MQ=255)                                                                                                                                  ttGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTAACCAg  <  2:428847/135‑1 (MQ=255)                                                                                                                                  ttGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTAACCAg  <  7:452847/135‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       GTGGTGGCCAGGTCGTCGGACAGCGGCACCAGGGTGTGCGCGGAGCCGGCATACACCACCAGCGCCGTCTGGCTGTCGCGGCGGTGTTCAAGCAGGTCGAGGATCTTGCGCCGGGCCTGTTCCAGGCGGTTGGGCGGGCTGTCCTCGGCGAGCATTTGCGGGGTCAGTTCGAGCAGGATCACCAGCGGGTCGGCCGGGCGCTGACGGCTTTCTTCCAGCCGCTGCCAGCTGGGCCCGAGCAGCGCCAGGATCACCAGTAACCAG  >  ACB122/2299147‑2299410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑