Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,358,743 T→G 25.3% L41R (CTG→CGG)  CJ019_02230 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,358,7430TG25.3% 88.3 / 3.2 56L41R (CTG→CGG) CJ019_02230hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/31);  new base G (10/6);  total (19/37)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTATTGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGGATCGATCGCTTTGCC  >  ACB122/2358612‑2358873
                                                                                                                                   |                                                                                                                                  
gggTATTGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGa                                                                                                                                 >  4:355915/1‑135 (MQ=255)
gggTATTGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTGCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGa                                                                                                                                 <  4:271677/135‑1 (MQ=255)
      tGCCGTGAACCTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCTCGAACTGGCGCGGCGGGAGCAGGt                                                                                                                           <  3:14703/135‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAgg                                                                                                                            <  4:327971/134‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAgg                                                                                                                            <  6:389491/134‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAgg                                                                                                                            <  7:162298/134‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGt                                                                                                                           <  7:41609/135‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGt                                                                                                                           <  3:415023/135‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGt                                                                                                                           <  6:79138/135‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGt                                                                                                                           <  1:398959/135‑1 (MQ=255)
      tGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGGCAGCCGCGAACTGGCGCGGCTGGAGCAGGt                                                                                                                           <  7:205869/135‑1 (MQ=255)
          gTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCCGCCGACCGTCCGCCGCGCACTGGCGCGGCGGGGGCCgggggct                                                                                                                       >  8:41609/1‑130 (MQ=255)
          gTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACt                                                                                                                       <  7:428194/135‑1 (MQ=255)
          gTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACt                                                                                                                       <  5:346845/135‑1 (MQ=255)
          gTGAAACTGTCCGTCCGCCACATCGAAGTGTTACGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTGCGGCGCGAACAGGGGCCGGGAGAGCAGGTGAcc                                                                                                                       >  5:214086/1‑134 (MQ=255)
              aaCTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCCCCCACAGGCGCGGCGGGCGCAGGGGACTgggc                                                                                                                   >  1:242771/1‑133 (MQ=255)
                cTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCGGGAGCAGGTGACGGGCCTg                                                                                                                 >  6:183282/1‑135 (MQ=255)
                      gtccgCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGCCCGGGCGCGGCGGGGGCAGGGGGCTGGCCGGGAccgg                                                                                                           >  1:255904/1‑133 (MQ=255)
                      gtccgCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTg                                                                                                           <  2:76861/135‑1 (MQ=255)
                              caTCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCCGCCGCGCACTGGCGCGGCGGGCGCAGGTGGCTGGGCTGGACCGGGTCGAacg                                                                                                   >  2:352250/1‑135 (MQ=255)
                                 cGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGa                                                                                                <  3:355915/135‑1 (MQ=255)
                                 cGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGGGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGATCGAGCGCGa                                                                                                <  7:55574/135‑1 (MQ=255)
                                           cgcgCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCCGCCGCGCCCAGGCGCGGGGGGGGCCGGGGGCGGGGCTGGACCCGGTTGGGCGCGGGGGCGGgcgc                                                                                      >  3:27349/1‑135 (MQ=255)
                                             cgcCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGCACTGGCGCGGCTGGAGCAGGGGACTGGCCTGCACCTGGTCGAGCGCGAGGGCGGGCGccg                                                                                    >  8:383636/1‑134 (MQ=255)
                                                catcatGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCACCCCCGGACAGGCGCCGCGGGCGCAGGTTACGGGCCAGAACCTGTTGGAGCGCGAGGGCGGGCGCCgggt                                                                                 >  4:62427/1‑135 (MQ=255)
                                                  tcatGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCtggtgg                                                                               <  8:379265/135‑1 (MQ=255)
                                                  tcatGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGGCAGCCGCGAACTGGCGCGGCGGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCtggtgg                                                                               <  4:159018/135‑1 (MQ=255)
                                                  tcatGGCCGCTGGCAGGGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCtggtgg                                                                               <  6:413849/135‑1 (MQ=255)
                                                     tGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGCCCGTCCCCCGCGAACAGGCGCGGCGGGGGCCGGGGGCGTGGCGGGCCCAGGTCGCGCGCGAGGGCGGGCGGCGGGGGGCgg                                                                            >  2:95862/1‑134 (MQ=255)
                                                     tGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCCCGCACTGGCGCGGCTGGGGCAGGGGGCGGGCCTGAACCTGGTGGAGCGCGCGGGCGGGCGGCgggtggggg                                                                            >  8:462865/1‑132 (MQ=255)
                                                        ccGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGGCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGGTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACgg                                                                         <  3:62427/135‑1 (MQ=255)
                                                             ggCAGTGGCACGGGGGCGGCGCGCCTGCTGTGCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAg                                                                    <  8:29587/135‑1 (MQ=255)
                                                               cAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGGCGCGAACTTGCGCGGCGGGAGCAGGGGTCTGGCCTGACCCTGTTGGAGCGCGCGGGCGGGCGCCGGGTGGCGCCGGCCCCGgc                                                                  >  8:135688/1‑135 (MQ=255)
                                                                            gcggcgCGCCTGCTGTTCCCCTCGCAGCCGACCGTCCGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATc                                                     <  1:209554/135‑1 (MQ=255)
                                                                            gcggcgCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATc                                                     <  6:223152/135‑1 (MQ=255)
                                                                                 gcgcCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGCAATGGCGCGGCTGGAGCAGGTGCCTGGCCTGAACCTGTTCGAGCGCGTGGGCGGGCGCCTGGTGGGGACCGCCCCCGCGCGGCGGGTGCTCGgaga                                                >  5:157845/1‑135 (MQ=255)
                                                                                     ctgctgTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTc                                            <  6:128772/135‑1 (MQ=255)
                                                                                     ctgctgTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTc                                            <  4:235671/135‑1 (MQ=255)
                                                                                     ctgctgTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTc                                            <  2:242771/135‑1 (MQ=255)
                                                                                       gctgTTCACCTCGCAGCCGACCGTCCGCCGCGAACTGGCGCGGCTGGCGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGCCGGCCCAGGCGCTGCGGGTGATAGCAGAGGTCgc                                          >  7:288585/1‑134 (MQ=255)
                                                                                               ccTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCt                                  <  3:286602/135‑1 (MQ=255)
                                                                                                cTCGCAGCCGACCGTACGCCGCGACGTGGCGCGGCTGTAGCAGGTTACTGGCCTGAACCTGTTCGAGCGCGTGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTa                                 <  2:188363/135‑1 (MQ=255)
                                                                                                                  ccGCGCACTGGCGCGGCTGGAGCAGGTGACTGGCCGGAACCTGTTCGAGCGCGAGGGCGGGCGCCGGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgag               <  5:307564/135‑2 (MQ=255)
                                                                                                                       cccTGGCGGGGCTGTAGCCGGTGACTGGCCGGCACCTGTTCGCGCGCGCGGGCGGGGGCCTGGGGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  1:214040/133‑1 (MQ=255)
                                                                                                                       caCTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGGGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  7:431819/134‑1 (MQ=255)
                                                                                                                       acgtGGCGGGGGGGGTGCCGGTGACGGGCCTGACCCTGTTCGAGGGCGCGGGCGGGCGCCTGGGGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  5:264288/132‑1 (MQ=255)
                                                                                                                       accTGGCGGGGCGGGAGCAGGTGTCGGGCCTGAACCTGTGGGAGCGCGCGGGCGGGCGCCGGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  5:375976/133‑1 (MQ=255)
                                                                                                                       aaCTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  8:298943/135‑1 (MQ=255)
                                                                                                                       aaCTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  2:399232/135‑1 (MQ=255)
                                                                                                                       aaCTGGCGCGGCGGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  1:420630/135‑1 (MQ=255)
                                                                                                                       aaCGGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTGCGAGCGCGAGGGCGGGCGCCTGGGGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGgatcgatc          <  5:225866/135‑1 (MQ=255)
                                                                                                                        aCTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCGGGTGGCGGCCGCCCCGGCGCGGGTGTTGCTCGAACAGGTCGCGCGGGCCTACGTCGGGCGGGAGCGGctcgctcg         >  2:164363/1‑135 (MQ=255)
                                                                                                                        aCTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTAGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGGatcgatcg         >  4:322220/1‑135 (MQ=255)
                                                                                                                             cgcgGCGGGAGCAGGGGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGGATCGATCGCTTTg    <  7:55125/135‑1 (MQ=255)
                                                                                                                               cgGCTGGAGCAGGTGACTGGCCTGACCCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGGCTCGATCGCTTTGcc  >  7:280700/1‑135 (MQ=255)
                                                                                                                               cgGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGCCGGCCCAGGCGCTGCTGGTGATCGAAGAGGTCGAGCGGCCCTACGTCGGGCTGGAGCGGCTCGATCGCTTTGcc  >  8:237916/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                  
GGGTATTGCCGTGAAACTGTCCGTCCGCCACATCGAAGTGTTCCGCGCCATCATGGCCGCTGGCAGTGTCACGGGGGCGGCGCGCCTGCTGTTCACCTCGCAGCCGACCGTCAGCCGCGAACTGGCGCGGCTGGAGCAGGTGACTGGCCTGAACCTGTTCGAGCGCGAGGGCGGGCGCCTGGTGGCGACGGCCCAGGCGCTGCTGTTGATCGAAGAGGTCGAGCGGGCCTACGTCGGGCTGGAGCGGATCGATCGCTTTGCC  >  ACB122/2358612‑2358873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑