| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,364,172 | C→G | 6.6% | T1311T (ACC→ACG) | gdhB → | NAD‑specific glutamate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,364,172 | 0 | C | G | 6.6% | 174.4 / 2.9 | 57 | T1311T (ACC→ACG) | gdhB | NAD‑specific glutamate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (27/26); new base G (2/2); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGTCC > ACB122/2364039‑2364296 | gCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCTGACGACGACTACCTGACCCGGGCAATGGAAAcc > 2:406277/1‑134 (MQ=255)gCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCg > 2:58924/1‑135 (MQ=255) cTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTcc < 7:329843/135‑1 (MQ=255) cTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGGGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTcc < 7:150090/135‑1 (MQ=255) ccAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGCCCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTc > 1:90590/1‑135 (MQ=255) aCCCGCGGCGAGCTGTCGGTGCTGATCTCGTACAGCAAGAGCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGc < 1:437943/135‑1 (MQ=255) ccGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCaa > 7:304588/1‑135 (MQ=255) ccGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCaa > 3:6947/1‑135 (MQ=255) gcCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGGCTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTc < 3:211824/135‑1 (MQ=255) ccGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCg < 1:146815/135‑1 (MQ=255) cGAGCTGTCGTTGATGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGATCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGAACCTGCTCAGCAAGTTCGc > 6:159378/1‑135 (MQ=255) cGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGc > 6:83649/1‑135 (MQ=255) tGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAgg < 4:335333/135‑1 (MQ=255) gTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATg > 3:264952/1‑135 (MQ=255) gTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATg > 7:221740/1‑135 (MQ=255) tGATCTCGTACAGCAAGATCGACCTCAAGGAGCTACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGcgcc < 5:344112/135‑1 (MQ=255) aTCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGcgccgc > 4:296699/1‑135 (MQ=255) tctcGTACAGCAAGATCGACCTCAAGGAGCAACTGCTAAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAACCCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCgccgcc > 1:373071/1‑135 (MQ=255) cAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCTAGTTCGCCGAGGCCATGCGCCGCCACCGCCt > 5:11059/1‑135 (MQ=255) aGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGTCTACCTGACCCGTGACTTGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAgc > 1:403151/1‑135 (MQ=255) aTCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAgcgc < 8:272752/135‑1 (MQ=255) aTCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAgcgc < 1:130302/135‑1 (MQ=255) aTCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGAGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAgcgc < 6:454258/135‑1 (MQ=255) gACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGa < 1:406277/134‑1 (MQ=255) tCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGcgcc > 1:179830/1‑111 (MQ=255) tCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGcgcc < 2:179830/111‑1 (MQ=255) aCTGCTCAAGTCGCTGGTGCCGGAGGAGGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCAACCGCCTGAAGCGCGAAATCGTCAGCACCCa < 1:277896/135‑1 (MQ=255) cTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCAc > 1:307817/1‑131 (MQ=255) cTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCAc > 8:197767/1‑131 (MQ=255) tCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGa > 1:166869/1‑115 (MQ=255) tCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGa < 2:166869/115‑1 (MQ=255) tCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCg > 6:398204/1‑135 (MQ=255) aaGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCGCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCa < 2:398046/121‑1 (MQ=255) aaGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCa > 1:398046/1‑121 (MQ=255) aaGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGc > 3:439711/1‑134 (MQ=255) aGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAACCCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCa < 3:296699/135‑1 (MQ=255) aGTCGCTGGGGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCGGGCCGTCCTTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACGCAGATCGcaa < 6:63159/135‑3 (MQ=255) gTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCaa > 4:375831/1‑135 (MQ=255) tCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAAc < 5:398204/135‑1 (MQ=255) cgatGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACg < 8:141682/132‑1 (MQ=255) gCTGGTGCCGGACGACGACTACCTGCCCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGa > 1:33245/1‑135 (MQ=255) gCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACGGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCAt < 6:247407/84‑1 (MQ=255) gCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACGGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCAt < 6:252934/84‑1 (MQ=255) gCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACGGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCAt > 5:252934/1‑84 (MQ=255) gCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACGGCTTTCCCGCCGTCCCGGGTCAGCAAGTTCGCCGAGGCCAt > 5:247407/1‑84 (MQ=255) gacgacgacTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTcaac < 6:1648/135‑1 (MQ=255) acgacgacTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTcaaca < 1:86292/135‑1 (MQ=255) acgacgacTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTcaaca < 3:375831/135‑1 (MQ=255) gACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTcaaca < 3:419872/122‑1 (MQ=255) gACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTcaaca > 4:419872/1‑122 (MQ=255) ccGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCCCCGCCTGAAGCGCGAAATCGTCAGCACCCAGCTCGCCAACGCCCTGGTCAACAACATGCGTATCACCtt > 2:251331/1‑135 (MQ=255) gTGACTTGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCg < 2:403151/135‑1 (MQ=255) tGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGt < 8:304588/135‑1 (MQ=255) tGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTGCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGt < 5:424236/135‑1 (MQ=255) aCATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGCCCTGGTCAACAACATGGGTATCACCTTCGt > 5:399452/1‑133 (MQ=255) aCATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGt > 5:366564/1‑133 (MQ=255) aCATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGt > 8:315837/1‑133 (MQ=255) aCATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGATCCGCCACCGCCTGAAGCTCGAAATCGTCAGACCACAGATCTCCAACTACCTGGTCAACAACATGGGTATCACCTTCGTcc > 7:335066/1‑135 (MQ=255) | GCCTGGCTGCCGGCCAGGGCCTGACCCGCGCCGAGCTGTCGGTGCTGATCTCGTACAGCAAGATCGACCTCAAGGAGCAACTGCTCAAGTCGCTGGTGCCGGACGACGACTACCTGACCCGTGACATGGAAACCGCTTTCCCGCCGTCCCTGGTCAGCAAGTTCGCCGAGGCCATGCGCCGCCACCGCCTGAAGCGCGAAATCGTCAGCACCCAGATCGCCAACGACCTGGTCAACAACATGGGTATCACCTTCGTCC > ACB122/2364039‑2364296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |