| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,379,994 | T→G | 20.4% | Q56P (CAG→CCG) | nrtA ← | Nitrate/nitrite binding protein NrtA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,379,994 | 0 | T | G | 20.4% | 141.8 / 3.6 | 90 | Q56P (CAG→CCG) | nrtA | Nitrate/nitrite binding protein NrtA |
| Reads supporting (aligned to +/- strand): ref base T (30/38); new base G (18/4); total (48/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACGGCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAC > ACB122/2379860‑2380124 | gCACGGCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCt > 2:74746/1‑135 (MQ=255)gCACGGCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCt > 2:2903/1‑135 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCTCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATTCAGGCAATGTGCTGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCAGGAGCCGTGGCGCTGGAg < 2:144866/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGAGCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 5:262078/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 2:409345/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 8:180548/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 1:456404/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 6:192332/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 1:88945/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 5:398906/135‑1 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCCGCCCACGCGCCCTGGCGCGGGAg > 2:234955/1‑135 (MQ=255) ggCCATGTCGCTGGCAGGTACGCCGCCAATGACCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAg < 5:444132/135‑1 (MQ=255) cATGTCGCTGGAAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGATCCCTGTCGCTGGAGGtt > 2:369874/1‑135 (MQ=255) cGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCGGGCGCGGGAGGGTGAggg > 6:142730/1‑135 (MQ=255) gCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAgg < 2:186711/135‑1 (MQ=255) gCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAgg < 7:207846/135‑1 (MQ=255) cAGGTACGCCGCGAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGGGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAgg < 2:180779/134‑1 (MQ=255) aGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCGGGCGCGGGAGGTTGAGGGTAAGGcc > 6:360836/1‑135 (MQ=255) aGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGcc > 1:60192/1‑135 (MQ=255) gTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGGTGAGGGTAAGGCCgg > 4:339051/1‑134 (MQ=255) ccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTgg < 3:339364/135‑1 (MQ=255) ccAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTgg < 1:186500/135‑1 (MQ=255) cAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGGGGGTAAGGCCGTGCTGCTggg > 3:59463/1‑135 (MQ=255) ccAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAg < 2:357176/134‑1 (MQ=255) gTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGGGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTg > 8:442702/1‑135 (MQ=255) aCGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTg < 2:456959/135‑1 (MQ=255) aCGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTg < 7:277079/135‑1 (MQ=255) ggCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGGGCGGCTGGGCGAAGCCCTGGGTggc > 8:74603/1‑135 (MQ=255) gTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTggcggc < 3:407194/135‑1 (MQ=255) aGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCGGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGa > 5:429689/1‑135 (MQ=255) aCCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCGGGCGCTGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGAcc > 8:391240/1‑135 (MQ=255) aCCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGccc > 8:358632/1‑135 (MQ=255) ccAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCa > 7:266197/1‑135 (MQ=255) ggCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGa < 7:64718/135‑1 (MQ=255) ccATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCATGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGGTGACGGTAAGGCCGTGCAGCTCGCCGAAACCCTCGGAGGCGGCGCCcaccac > 8:158388/1‑134 (MQ=255) cATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCGGGCGCGGGCGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGGGGCGGCGCCCCCGAc > 1:144513/1‑133 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCCGCTTGTCGCGCCGCCCCGCCCCCCCCCCCGGGGGCGGGAGGGTGTGGGGATGGGCGGGGGGGGGGGGGCAGGCCGGGGGGGCGGCGGCCAccccccc > 7:167085/1‑129 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCCCCCCCGCCCCCCCCCCCTGGCGCGGGAGGTTGAGGGTTAGGCCCTGGTGCTGGGCGGAGCCCAGGGTGGCGGCGCCCCCCAcccc > 3:426510/1‑133 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCccgaccga > 4:219978/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCGGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGCAGCCCGGGGTGGCGGCGCCCACCCCCGa > 7:8805/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGCGCTGGGGGGTGAGGGTAAGGCCGTGCTGCTGGGCGCAGCCCTGGGTGGCGGCGCCcccgcccgc > 8:454588/1‑134 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 5:217518/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 7:305449/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCAGGCGCTGGAGGTTGCGGGTAAGGCCGTGCTGCTGGGGGAAGCCCGGGGTGGCGGCGCCcccgcccgc > 6:13562/1‑134 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAgg > 4:86764/1‑85 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAgg < 3:86764/85‑1 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGCAGCCCTGGGTGGCGGCGCCCACGCCCGa > 2:373148/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCACGACCgc > 8:143907/1‑134 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCccgaccga > 4:325254/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 7:386008/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGa > 2:337777/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGCCCGa > 6:201294/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCGGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGGAGCCCGGGGTGGCGGCGACCACGACCGa > 8:250749/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCGGGCGCGGGAGGGTGAGGGTAAGGCCGGGCTGCTGGGCGCAGCCCTGGGTGGCGGCGCCCACGCCCgc > 6:309029/1‑134 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCCGCCCACCACCCCTGGCGCGGGAGGGTGAGGGTAAAGGCGAGGTGCTGGGGGAAGGCCAGGGGGGGGGCGGCCccgaccga > 5:408741/1‑135 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCACCCCGGCCCACCAACCCAGGCCCGCGACGTTGAGGGTAAAGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGAccca > 5:431466/1‑133 (MQ=255) aTACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCACCCCCGCCCACCCCCCCGGGCCCGGGAGGTGGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCaccaccca > 1:154848/1‑133 (MQ=255) aGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGACCACGACCGCGgc > 7:291516/1‑134 (MQ=255) aGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcg < 3:339051/135‑1 (MQ=255) aGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcg < 4:406378/135‑1 (MQ=255) aGGCAATGGGCGGCGTCCTGTTCGCCGCTGACCAGCTTGGCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGGGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcg < 4:426510/135‑1 (MQ=255) ggCAATGTGCGGCGTCCAGTCCGCCGCTGCCCAGCTGGGCGCGCAGCCCGGCCCACGAGCCGTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcgc < 4:239996/135‑1 (MQ=255) ggCAAGGTGCGGCGTCCAGTTCGCCGCGGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGTTGCGGGTACGGCCGGGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcgc < 2:218967/135‑1 (MQ=255) attggcggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCGCTGCCGCGGGAGGTTGAGGGTAAGGCGGTGCGGGGGGGCGAAGCCGTGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 2:127346/131‑1 (MQ=255) aTGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 7:232835/135‑1 (MQ=255) aTGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 3:220127/135‑1 (MQ=255) aTGTGCGGCGTCCAGTGCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 6:137777/135‑1 (MQ=255) aTGGGCGGCGGCCAGTGCGCGGCGGACCAGCTTGGCGCGCAGCCCGGCCCACGCGCCGGGGCGCGGGAGGTGGCGGGTACGGCCGGGCGGCGGGGCGAAGCCCGGGGGGGCGGCGACCACGACCGAGGCGCAGTc < 2:450953/135‑1 (MQ=255) tgtgCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTTGCGCGGGTGGTTGATGGTAAGGACGGGCTGCTGGGCGCGGCCCTGGGTGGCGGCGACCCCGACCGCGGCGCAGTCg > 6:338918/1‑135 (MQ=255) tgtgCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGGGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCCCCACCGAGCCGCAGTCg > 2:117329/1‑135 (MQ=255) gtgCGGCGTCCAGTTCGCCGCTGACCCGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGGTGAGGGTTAGGCCGTGCTGCTGGGCGAAGCCCGGGGTGGCGGCGCCCACGACCGAGGCGCAGTCgg > 2:253715/1‑135 (MQ=255) tgCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCGGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGt < 4:248127/135‑1 (MQ=255) gcggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCgg > 3:214821/1‑133 (MQ=255) cggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCCCGAGCCCTGGCGCTGGAGGTGGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGGGGCGGCGACCACGACCGAGGCGCAGTCGGTCa < 6:294416/135‑1 (MQ=255) cggcgTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCa < 4:59463/135‑1 (MQ=255) tCCAGTTCGCCGCTGACCAGCTTGTCGCGCCGCCCGGCCCCCGAGCCCTGGCGCGGGAGGGTGAGGGTAAGGCCGTGCGGCTTGGCGCAGCCCGGGGGGGCGGCGGCCCCGACCGCGGCGCCGGCGGGCAGGGc > 2:398547/1‑134 (MQ=255) tCCAGTTCGCAGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGCGCCCTGGCGCTGGAGGGTGAGGGGAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGCCCACGCCCGCGGCGCCGTCgggccgggcc > 8:146734/1‑135 (MQ=255) ccAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGGTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGcc > 7:153876/1‑134 (MQ=255) ggCCCACGCGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGACGCCCTGGGTGGCGGCGACCACGCCCGAGGCGCAGTCGGTCAGGGCCATTTAGACGAGGTGCAGGCTGGTGTTTTCCGgcgc > 5:308081/1‑135 (MQ=255) gCCCACGAGCCCGGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGcgcg < 8:8805/135‑1 (MQ=255) aCGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGc > 3:182188/1‑135 (MQ=255) aGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGGGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGc < 7:358632/135‑1 (MQ=255) tGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGTCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 6:180053/135‑1 (MQ=255) tGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 8:153876/135‑1 (MQ=255) tGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 8:157922/135‑1 (MQ=255) tGGCGCGGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 5:309029/135‑1 (MQ=255) gggCGCTGGTGGTTGAGGGTAAGGCCGGGCGGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGTCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTg < 6:431466/134‑1 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcg > 2:154948/1‑70 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGgcg < 1:154948/70‑1 (MQ=255) gcgcTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAc > 4:65161/1‑135 (MQ=255) cTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTc < 3:49259/72‑1 (MQ=255) cTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTc > 4:49259/1‑72 (MQ=255) | GCACGGCCATGTCGCTGGCAGGTACGCCGCCAATGCCCAGGTGCACGGCGTAGACCAGGCCATACAGGCAATGTGCGGCGTCCAGTTCGCCGCTGACCAGCTTGTCGCGCAGCCCGGCCCACGAGCCCTGGCGCTGGAGGTTGAGGGTAAGGCCGTGCTGCTGGGCGAAGCCCTGGGTGGCGGCGACCACGACCGAGGCGCAGTCGGTCAGGGCCATATAGCCGATGTTCAGGCTGGTTTTTTCCGGCGCGTCGCTGCCGTTGAC > ACB122/2379860‑2380124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |