Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,416,103	C→T	5.6%	R377H (CGC→CAC)	CJ019_02281 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,416,103	0	C	T	5.6%	207.4 / 3.0	75	R377H (CGC→CAC)	CJ019_02281	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/40);  new base T (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

ATCCCCTTCAGCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAC  >  ACB122/2415970‑2416233                                                                                                                                       |                                                                                                                                   aTCCCCTTCAGCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCTACGCTCCAGTCGT‑Ggcg                                                                                                                                   >  2:185582/1‑135 (MQ=255) aTCCCCTTCAGCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑Ggc                                                                                                                                    <  1:270141/134‑1 (MQ=255)      cTTCAGCGAGCTACTTGGCTGAGCGGCCCCGCCAGGCACTGGCCAGCGCCGCGGCCACGTCCGTTGCCGCCTGGTTCAGGCGTATGTGAACATGCGCCAGTTGTGGGGCCCCCACGCTCCCGACTT‑GGCGCTGGc                                                                                                                              >  7:146463/1‑135 (MQ=255)           gCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGc                                                                                                                          >  2:111134/1‑134 (MQ=255)           gCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGc                                                                                                                          >  7:244296/1‑134 (MQ=255)           gCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGATCTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTCGCGGGc                                                                                                                          >  7:449837/1‑134 (MQ=255)                  aCTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGTGCTGGCGGGCAAACTGgc                                                                                                                  >  7:81434/1‑135 (MQ=255)                  aCTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGTGCTGGCGGGCAAACTGgc                                                                                                                  >  7:74105/1‑135 (MQ=255)                  aCTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCCAACTGgc                                                                                                                  >  1:49163/1‑135 (MQ=255)                  aCTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑AGCGCTGGCGGGCAAACTGgc                                                                                                                  >  1:202252/1‑135 (MQ=255)                   cTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGgcg                                                                                                                 >  1:332780/1‑135 (MQ=255)                    ttGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCGCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGgc                                                                                                                  <  3:300962/133‑1 (MQ=255)                           aGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCAt                                                                                                         <  2:180908/135‑1 (MQ=255)                              ggCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGc                                                                                                      >  7:104568/1‑135 (MQ=255)                                cccAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCGGGCGGGCAAACTGGAGCCCGGCATCGcgc                                                                                                    >  8:94514/1‑133 (MQ=255)                                cccAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCGCCAGTCGT‑GGCGCGGGCGGGCCAACTGGCGCCCGGCATCGCt                                                                                                     >  7:72283/1‑134 (MQ=255)                                 ccagccagGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCa                                                                                                   <  7:109105/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  1:381184/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  2:332780/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  6:243109/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  5:107298/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑CGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  2:202252/135‑1 (MQ=255)                                       agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTGGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGc                                                                                             <  8:38423/135‑1 (MQ=255)                                             tGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCTACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                       <  1:185582/135‑1 (MQ=255)                                             tGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                       <  7:228795/135‑1 (MQ=255)                                             tGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                       <  4:227030/135‑1 (MQ=255)                                             tGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATCTGCACATTCTCCAGTTGTTGGGCAGCCGCGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                       <  5:223226/135‑1 (MQ=255)                                             tGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCGGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAAATGGCGCCCGGCCTCGCGCAACAGGcggctgc                                                                                       >  4:330285/1‑129 (MQ=255)                                                  aGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGGCGCTGGCGGGCAACCTGGCGCCCGGCATCGCTCACCTGGGGCATGCGccc                                                                                   >  8:9709/1‑134 (MQ=255)                                                  aGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCGCATGCGCCAg                                                                                  >  1:86281/1‑135 (MQ=255)                                                   gcggcgcggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAgg                                                                                 <  6:346828/135‑1 (MQ=255)                                                       cgcggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCCCATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGGTGGCGGGCAAAATGGCGCCCGGCCTCGCTCAACTGGCTCATGCGCCAGGGccg                                                                             >  2:192044/1‑133 (MQ=255)                                                          ggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGAtt                                                                          <  7:139905/135‑1 (MQ=255)                                                          ggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGAtt                                                                          <  6:397051/135‑1 (MQ=255)                                                          ggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGAtt                                                                          <  5:191712/135‑1 (MQ=255)                                                            ccTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGa                                                                        >  4:323969/1‑135 (MQ=255)                                                            ccTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTCGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGa                                                                        >  3:374333/1‑135 (MQ=255)                                                             cTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGag                                                                       <  2:342371/135‑1 (MQ=255)                                                                 tCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCGCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGagca                                                                     <  8:5441/133‑1 (MQ=255)                                                                       gccgccTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTg                                                             <  4:12926/135‑1 (MQ=255)                                                                         cgccTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGa                                                            >  5:423918/1‑134 (MQ=255)                                                                         cgccTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGAt                                                           <  7:220907/135‑1 (MQ=255)                                                                           ccTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCa                                                         <  5:321763/135‑1 (MQ=255)                                                                             tGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCGCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGc                                                       <  8:157348/135‑1 (MQ=255)                                                                              gggTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCa                                                      >  1:13934/1‑135 (MQ=255)                                                                              gggTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCa                                                      >  4:380206/1‑135 (MQ=255)                                                                                  cAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGc                                                  <  4:409838/135‑1 (MQ=255)                                                                                        tATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTg                                            >  4:74953/1‑135 (MQ=255)                                                                                             gCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCa                                        <  1:273848/134‑1 (MQ=255)                                                                                                  ttCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcg                                  >  2:255408/1‑135 (MQ=255)                                                                                                  ttCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcg                                  >  8:368490/1‑135 (MQ=255)                                                                                                  ttCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcg                                  <  1:50419/135‑1 (MQ=255)                                                                                                  ttCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGAGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATAACcgtc                                   >  4:446597/1‑134 (MQ=255)                                                                                                   tctcCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtggt                                 <  5:33599/135‑1 (MQ=255)                                                                                                   tctcCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcgt                                 <  5:234882/135‑1 (MQ=255)                                                                                                   tctcCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcgt                                 <  3:454509/135‑1 (MQ=255)                                                                                                   tctcCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcgt                                 <  6:220909/135‑1 (MQ=255)                                                                                                   tctcCAGTTGGTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACcgtcgt                                 <  4:333806/135‑1 (MQ=255)                                                                                                    ctcCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTTATCACCgtcgtc                                >  6:19744/1‑135 (MQ=255)                                                                                                      ccAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTTGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGCTTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCAc                              >  6:140094/1‑135 (MQ=255)                                                                                                        aGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCa                               <  4:217684/132‑1 (MQ=255)                                                                                                        aGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTg                            <  2:148095/135‑1 (MQ=255)                                                                                                        aGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTg                            <  8:244296/135‑1 (MQ=255)                                                                                                           tgttgGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGccc                         <  6:423918/135‑1 (MQ=255)                                                                                                                  cAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAagtagt                  >  2:15602/1‑135 (MQ=255)                                                                                                                  cAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCGCATGCGCCAGGGCTGATTGAGCCGCTGGGTGCTCAGCAATGCCAGTTGATCACCGGCGTGCCTGCCCCagtagt                  >  6:423421/1‑135 (MQ=255)                                                                                                                       aCGCTCCAGTCGT‑GGGGCTGGCGGGCAAACTGGCGCCCGGCCTCGCGAAACTGGCTAATGCGCCAGGGCGTATTGAGCAGCAGGGTGCTCAGCCATGCCCGTTGATAACCGGCGTCACAGCCCAACTAGTGTTCg             >  2:200021/1‑135 (MQ=255)                                                                                                                       aCGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCg             <  6:311124/135‑1 (MQ=255)                                                                                                                       aCGCTCCAGTCGT‑GGCGCGGGCGAGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCg             <  1:179957/135‑1 (MQ=255)                                                                                                                        cGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCGAGTTGATCACCGTCGTGCCTGCCCAAGTAGGGTTCGc            >  5:143499/1‑135 (MQ=255)                                                                                                                        cGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGc            >  5:377061/1‑135 (MQ=255)                                                                                                                                  gT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAc  <  3:334154/135‑1 (MQ=255)                                                                                                                                  gT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAc  <  5:243204/135‑1 (MQ=255)                                                                                                                                  gT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTACGCCGTTGTTCAc  <  2:76445/135‑1 (MQ=255)                                                                                                                                  gT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAAATGGCTAATGCGCCAGGGCGGATTTAGCAGCTGGGTGATCAGCAATTCCAGTTGATCAACGTCGTCAATGCCCAATTAGGGTTCGCCgtttgtcaa  >  1:277553/1‑129 (MQ=255)                                                                                                                                   t‑GGTGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAc  <  8:74105/134‑1 (MQ=255)                                                                                                                                   t‑GGTGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAc  <  8:81434/134‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   ATCCCCTTCAGCGAGCTACTTGGCTGAGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGT‑GGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCAC  >  ACB122/2415970‑2416233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑