Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,526,900	C→T	5.4%	Q88Q (CAG→CAA)	CJ019_02384 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,526,900	0	C	T	5.4%	243.3 / 3.5	76	Q88Q (CAG→CAA)	CJ019_02384	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (45/26);  new base T (2/2);  total (48/28)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

CAGCACACTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCATGCTGCGGGCGCAGGGGCGC  >  ACB122/2526768‑2527031                                                                                                                                     |                                                                                                                                    cAGCACACTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGAGATCATCGACAGCGCTGCCAGTCCGAGGGGGTCAAGCAATGCCAATGGTATGTTAACGAACGCAGAGCGTGGCGgct                                                                                                                                    >  5:176579/1‑134 (MQ=255)   gCACACTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGgctgct                                                                                                                                 <  8:268534/135‑1 (MQ=255)   gCACACTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGgctgct                                                                                                                                 >  2:58037/1‑135 (MQ=255)     acacGGCGGTCCCGGGCCAGTGCGGACCAGACTGGGCCAGGCACCACGGGGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTAGGTGAACGAACGCAGAGCGTGGCGGCTGCTTg                                                                                                                               <  6:28235/135‑1 (MQ=255)       acTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTc                                                                                                                             >  2:102613/1‑135 (MQ=255)       acTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGGGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTc                                                                                                                             <  1:91375/135‑1 (MQ=255)           cGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCgggc                                                                                                                         >  5:151541/1‑133 (MQ=255)            ggTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGc                                                                                                                          <  3:287410/133‑1 (MQ=255)            ggTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGc                                                                                                                          <  1:58037/133‑1 (MQ=255)            ggTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGc                                                                                                                          <  5:66463/133‑1 (MQ=255)             gTCCCGTGCCAGTCCGGACCAGACGGGGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAAGGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCg                                                                                                                       <  5:73082/135‑1 (MQ=255)                    gCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCg                                                                                                                            >  4:242005/1‑123 (MQ=255)                    gCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCg                                                                                                                            <  3:242005/123‑1 (MQ=255)                      cAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCAcacc                                                                                                              >  7:138620/1‑135 (MQ=255)                      cAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCAcacc                                                                                                              >  6:205547/1‑135 (MQ=255)                      cAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCGTGTCGGCCCGCAGCAcacc                                                                                                              >  1:73203/1‑135 (MQ=255)                      cAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCGTGTCGGCCCCCAGcaccac                                                                                                              >  7:390456/1‑132 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGTTGCTTGTCGGCCCGCAGCACACCACggg                                                                                                         >  3:431399/1‑135 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGTTGCTTGTCGGCCCGCAGCACACCACggg                                                                                                         >  3:438697/1‑135 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCCGCACCCCACggg                                                                                                         >  8:276427/1‑135 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCCGCACCCCACggg                                                                                                         >  1:11591/1‑135 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACCCCACggg                                                                                                         >  1:346023/1‑135 (MQ=255)                           cGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAAAGGTATGTGAACGAACGCAGCGCGTGGCGCCTGCTTGTAGGCCCGCAGCACACCACgcg                                                                                                         >  7:455264/1‑133 (MQ=255)                               ccAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGTTGCTTGTCGGCCCGCAGCACACCACGGGccac                                                                                                     <  4:438697/135‑1 (MQ=255)                               ccAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGTTGCTTGTCGGCCCGCAGCACACCACGGGccac                                                                                                     <  4:431399/135‑1 (MQ=255)                               ccAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGccac                                                                                                     <  7:244665/135‑1 (MQ=255)                               ccAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGccac                                                                                                     <  6:388372/135‑1 (MQ=255)                                cAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGccac                                                                                                     <  6:148718/134‑1 (MQ=255)                                         ccAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTg                                                                                           <  7:458842/135‑1 (MQ=255)                                         ccAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTg                                                                                           <  2:379644/135‑1 (MQ=255)                                          cAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCCCACCACGGGCCACCAGCAGGtt                                                                                            >  6:314764/1‑133 (MQ=255)                                          cAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCCCGGGCCACCAGCAGGtt                                                                                            >  4:43293/1‑133 (MQ=255)                                          cAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCCCGCGCCACCACCAGGtt                                                                                            >  4:51304/1‑133 (MQ=255)                                          cAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCCCCAGCAGGtt                                                                                            >  7:139376/1‑133 (MQ=255)                                          cAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCGTGTAGGCCCGCAGCACACCCCCGGCCACCAGCCgggt                                                                                            >  8:42543/1‑131 (MQ=255)                                           aGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCCCCAGCAGGTTGGa                                                                                         >  3:379736/1‑135 (MQ=255)                                              caccccGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGt                                                                                      <  5:283640/135‑1 (MQ=255)                                              caccacGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGt                                                                                      <  8:63119/135‑1 (MQ=255)                                               accacGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGGTCGTTTTAGGCCCGCCGCACACCACCGGCCACCCGCACCGTGGAgg                                                                                       >  2:435624/1‑133 (MQ=255)                                               accacGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGGTGGAgggg                                                                                     >  5:205059/1‑133 (MQ=255)                                                ccacGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCCCGGGCCACCAGCAGGTTGGAGGTGa                                                                                    >  8:390611/1‑135 (MQ=255)                                                    ggtggtGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTTTAGGCCCGCCGCCCCCCACGGGCCCCCCCCCGGTTGGAGGTGCTca                                                                                 >  8:179847/1‑134 (MQ=255)                                                    ggtggtGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGGTGGAGGTGATgac                                                                                >  8:430472/1‑135 (MQ=255)                                                    ggtggtGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGACCGTGGCGGCGGCGTTTGCGCCCGCCGCCCACCCCGGGCCCCCAACAGTGTGGAGGTGGTca                                                                                 >  4:312360/1‑134 (MQ=255)                                                           atcatcGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCCCCCCACGGGCCCCCAGCAGGGTGGAGGGGCTCACATCGATg                                                                         >  1:78517/1‑135 (MQ=255)                                                           atcatcGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCCCACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATg                                                                         >  5:101619/1‑135 (MQ=255)                                                            tcatcGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCCCACCCCGGGCCCCCAGCAGGTTGGAGGGGATCACATccaagc                                                                        >  8:17354/1‑130 (MQ=255)                                                              atcGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCg                                                                      >  7:453344/1‑135 (MQ=255)                                                              atcGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCACCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCg                                                                      >  6:192190/1‑135 (MQ=255)                                                                   cAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCg                                                                 >  3:277286/1‑135 (MQ=255)                                                                     gcgcTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGc                                                                                                                          <  1:124673/76‑1 (MQ=255)                                                                     gcgcTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGc                                                                                                                          >  2:124673/1‑76 (MQ=255)                                                                         tGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCCGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAgct                                                           >  1:14758/1‑133 (MQ=255)                                                                         tGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGa                                                            >  5:152418/1‑134 (MQ=255)                                                                         tGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGa                                                            >  3:401688/1‑134 (MQ=255)                                                                           ccAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTAGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCagaaga                                                         >  8:163893/1‑135 (MQ=255)                                                                            cAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATg                                                          <  6:152418/133‑1 (MQ=255)                                                                            cAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATg                                                          <  4:401688/133‑1 (MQ=255)                                                                              ggCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTGGGCCCGCAGCCCACCACGGGCCCCCCGCAGGGTGGTGGGGTTAAAATAGCTGCCGACCCGGCAGTTGTggg                                                      >  5:392911/1‑134 (MQ=255)                                                                                   tGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCCCGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAggcgggcg                                                 >  2:56607/1‑135 (MQ=255)                                                                                      gggTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTg                                                                                           <  4:68710/90‑1 (MQ=255)                                                                                      gggTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTg                                                                                           >  3:68710/1‑90 (MQ=255)                                                                                      gggTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATg                                              <  3:1975/135‑1 (MQ=255)                                                                                        gTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTTTAGGCCCGCACCCCACCACCGGCCACCCGCAGGGTTGAGGTGATCACATCCATTCCCACCCGGCAGATGAGGCGGGCGAAGGc                                            >  7:328371/1‑135 (MQ=255)                                                                                            cGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGAGCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAgg                                        <  8:102125/135‑1 (MQ=255)                                                                                                   ccACTGGTATGTGCACGAACGCAGAGCGTGGCGGCTGCGTGTCGGCCCGCAGCACACCACGGGACACCAGCAGGTTGGAGGGGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGcc                                 <  2:356069/135‑1 (MQ=255)                                                                                                     aaTGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGt                               >  8:4976/1‑135 (MQ=255)                                                                                                     aaTGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACAACGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCgg                               >  4:162810/1‑134 (MQ=255)                                                                                                             gtgAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGtgtg                        <  8:328371/134‑1 (MQ=255)                                                                                                               gaacgaacGCAGAGCGTGGCGGCTGCTTTTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGACGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCAt                     >  6:358604/1‑135 (MQ=255)                                                                                                               gaacgaacGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCAt                     <  4:277286/135‑1 (MQ=255)                                                                                                               gaacgaacGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCAt                     >  5:438849/1‑135 (MQ=255)                                                                                                                cacgaacgGAGAGCGGGGCGGCTGCTTGGCGGCCCGCAGCACACCACGGGCCACCAGCAGGGTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGTTGGCGAGGGGGCGCCGTACGTGTGCAtg                    <  7:65380/134‑1 (MQ=255)                                                                                                                aacgaacgCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACAGCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCAtg                    <  8:138620/135‑1 (MQ=255)                                                                                                                 acgaacgCAGAGCGTGGCGGCATCATGTCGGCCCCCAGCACCCCACGGGCCACCAGCAGGTTGGACGTGAACACATCGCTCCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCAtgc                   >  3:62316/1‑135 (MQ=255)                                                                                                                     acgCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACCGGCCACCAGCAGGTTGGAGGTGATCTCAAAGATGCCGACCCCGCAGATGAGGCGGGGGATGGCGAGGGGGCGCCGTACGTGTGCATGCTGCg               >  8:433331/1‑135 (MQ=255)                                                                                                                       gCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCCCACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGCCCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCCTGCTGCggg             >  5:99982/1‑135 (MQ=255)                                                                                                                              gTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCCGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGGACGTGGGCATGCTGCGGGCGCAggg      >  2:180365/1‑135 (MQ=255)                                                                                                                              gTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCATGCTGCGGGCGCAggg      <  6:151541/135‑1 (MQ=255)                                                                                                                                  cggcTGCTTGTCGGCCCGCCGCCCACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGCCCCCGCAGATGAGGCGGGCGCTGGCGAGGGGGCGGCGTACGTGTGCATGATTCGGGCGCAGGGgcgc  >  5:367275/1‑135 (MQ=255)                                                                                                                                  cggcTGCTTGTCGGCCCGCAGCACCCCACGGGCCACCCGCAGGGTGGAGGTGTTCACATCGATGCCGACCCGGCAGATGAGGCGGGGGATGGCGAGGGGGCGCCGTACGTGTGCATGCTGCGGGCGCAGGGgcgc  >  8:79426/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                    CAGCACACTGCGGTCCAGTGCCAGTTCGGACCAGACTGTGCCAGGCACCACGGTGGTGATCATCGACAGCGCTGCCAGGCCGTGGGGGTCACGCAATGCCAATGGTATGTGAACGAACGCAGAGCGTGGCGGCTGCTTGTCGGCCCGCAGCACACCACGGGCCACCAGCAGGTTGGAGGTGATCACATCGATGCCGACCCGGCAGATGAGGCGGGCGATGGCGAGGGGGCGCCGTACGTGTGCATGCTGCGGGCGCAGGGGCGC  >  ACB122/2526768‑2527031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑