Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,569,872 A→C 38.2% L518R (CTG→CGG)  acoR_3 ← Acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,569,8720AC38.2% 20.1 / 4.0 37L518R (CTG→CGG) acoR_3Acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (17/5);  new base C (3/12);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 8.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAT  >  ACB122/2569744‑2569998
                                                                                                                                 |                                                                                                                              
ggTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGccc                                                                                                                          >  5:351269/1‑134 (MQ=255)
ggTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCCACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGCCGCCGACCGGCCCGTT‑GGGCGCCCCGGccc                                                                                                                          >  6:266073/1‑134 (MQ=255)
        ttGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGAcc                                                                                                                  >  4:83885/1‑135 (MQ=255)
              tGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGGGTTTTCGACTCGGCCAGAAATCCAGCTGGAAGGTGCTCGACGCCGACCGGCCCCTTGGGCCGCCAGGCCGAAC‑CGACCTGTaac                                                                                                            >  5:354697/1‑133 (MQ=255)
                   gACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCACCGCGACCTGTAGCACGc                                                                                                        >  4:206548/1‑134 (MQ=255)
                          gtctGCAGGTCGGTCTATTCCGTCGCACTCGGCACGGGTGTTTGCGACTCGCCCAGAACGCCGGCTGTACGGTGCGCGCCGCCGCCCGGCCCGTT‑GGCCGCCAGGCCCAACGCGACCTGTAGCACGCTCACCAGt                                                                                                <  8:42892/134‑1 (MQ=255)
                                      gTCTTTTCTGGCGCTAGCGGCCCGGGTGTTTTCGCCTCGGCCCCAACGCCCGCTGGATGGGGCTCGCCGCCGACGGGCCCGTG‑GGCCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTg                                                                                    <  4:312151/135‑1 (MQ=255)
                                       tctcTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCCGGGCCAACGCGCCCTGTAGCCCGCTCACCACTTCGCGCACGTTgg                                                                                   >  1:141829/1‑134 (MQ=255)
                                       tctcTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCACCGCGCCCTGTAGCACGCTCACCAGTTAGCGCAGGTTGc                                                                                   >  8:233042/1‑135 (MQ=255)
                                           tgcAGGCGCTATCGGCACCGGGGTTTTCGACTCGGCCAGAACGCCCGCTGGACGGGGCGCGACGCCGACCGGCCCGTT‑TGCCGCAAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCgg                                                                               <  4:373568/133‑1 (MQ=255)
                                              aggcGCTAGCGGCACCGGTGTTTGCGACTCGGCCAGAACGCCCGGTGGAAGGGGCGCGACGCCGCCCGGCCGGTG‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCgg                                                                               <  7:273278/129‑1 (MQ=255)
                                               gTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGCCCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAg                                                                           >  5:195335/1‑135 (MQ=255)
                                                tCGCTATCGCCACCGGTGTTTGCGACTCGGCAATAACGCCCGGTGTACGGGGCTCGACGCCGCCCGGCCCGTG‑GGCCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAgg                                                                          <  7:51174/135‑1 (MQ=255)
                                                   cTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGCCCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCACGGgt                                                                       >  4:74019/1‑135 (MQ=255)
                                                   cTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGgt                                                                       >  6:189421/1‑135 (MQ=255)
                                                   cTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGATCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCCACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCCGCCAGGGgt                                                                       >  4:278376/1‑135 (MQ=255)
                                                        ggCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGa                                                                  >  6:331892/1‑135 (MQ=255)
                                                          cACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGGGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATc                                                                <  4:63195/135‑1 (MQ=255)
                                                             cGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGa                                                              >  1:142870/1‑134 (MQ=255)
                                                                       gACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCACCGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCCGGGGTGTTGATCGAACAGGTCGATa                                                   >  7:406074/1‑135 (MQ=255)
                                                                               cAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGGCCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGGCAGGGGTGTTGATCGACCAGGGCGATAAAGGCcgg                                           >  7:271244/1‑135 (MQ=255)
                                                                                         gCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCCGGGCCCACGCGACCTGTAGCACGCACCCCAGTTCGCGCCGCTTGCCCGGCCACGGGGGGTGATCGACCAGGGGGATAATGGCCGGCGGCAGCcgc                                 >  3:417690/1‑135 (MQ=255)
                                                                                          cTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGCCCTGTAGCCCGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGCACAGGTCGATAATGGCCGGCGGCAGCcgc                                 >  7:287653/1‑134 (MQ=255)
                                                                                           tGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCcgc                                 >  3:225547/1‑133 (MQ=255)
                                                                                               aGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGt                           >  1:52436/1‑135 (MQ=255)
                                                                                                   gCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGcc                       >  7:154753/1‑135 (MQ=255)
                                                                                                        ccgccgcCCGCCCCGTG‑GGCCGCCCGGCCCACCGCGACCTGCAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGc                  <  5:245300/134‑1 (MQ=255)
                                                                                                        aCGCCCACCGGCCCGTG‑GGCCGCCCGGCCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCgg                    <  6:96266/133‑1 (MQ=255)
                                                                                                            cgcccGGCCCGTG‑GGCCGCCCGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTcgc              <  6:195335/132‑1 (MQ=255)
                                                                                                              cccGGCCCGTT‑GCCCGCCCGGCCCACCGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  6:412170/134‑1 (MQ=255)
                                                                                                              cccGGCCCGTG‑GGCCGCCCGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  2:9812/134‑1 (MQ=255)
                                                                                                              cccGGCCCGGG‑GGCCCCCCGGGCCACCGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGGTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  3:83885/134‑1 (MQ=255)
                                                                                                              aCCGGCCGGTG‑GGCCGCCCGGGCAACCGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGCACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  5:155555/135‑1 (MQ=255)
                                                                                                              aCCGGCCCGTG‑GGCCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  8:72184/135‑1 (MQ=255)
                                                                                                              aCCGGCCCGTG‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  6:193846/135‑1 (MQ=255)
                                                                                                              aCCGCCCCGTG‑GGCCGCCCGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCCCAGGTTGCCCGGGCTCGCgg            <  2:56112/135‑1 (MQ=255)
                                                                                                                         ggcccgccCGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAt  <  6:87325/132‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
GGTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTT‑GGCCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAT  >  ACB122/2569744‑2569998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑