Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,770,096	A→C	27.9%	W156G (TGG→GGG)	CJ019_02613 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,770,096	0	A	C	27.9%	92.0 / 5.1	67	W156G (TGG→GGG)	CJ019_02613	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base C (19/2);  total (41/26)
Fisher's exact test for biased strand distribution p-value = 2.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGG  >  ACB122/2769964‑2770230                                                                                                                                       |                                                                                                                                       gTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGAAATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAgg                                                                                                                                      <  5:243346/135‑1 (MQ=255)   cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCACCCGGC‑GCG‑CCCCCCCCCCCACACCCCCCAcgcc                                                                                                                                    >  3:237966/1‑134 (MQ=255)   cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTAGGTTGAACACCCGCACCCGCCCCGGTGCCAGCACCCACCCGGC‑GCG‑CCCCCCCCCCCACCCCGCCCAcgcc                                                                                                                                    >  8:192104/1‑134 (MQ=255)   cAAGTAGAGGCGCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAggcg                                                                                                                                    <  3:22623/135‑1 (MQ=255)      gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                                                 <  7:350681/135‑1 (MQ=255)      gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                                                 <  2:131030/135‑1 (MQ=255)        agagGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGCACCCCCGCACCCGCCCCGGTGCCTGCAGCCCCCGGGC‑GCG‑CCCCCCCCCCCACCCCCCCCACGCCGCCAt                                                                                                                               >  2:103228/1‑135 (MQ=255)               cAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGC‑GCG‑CCCCCCCCCCAACCCCGCCCCGGCGGCCCTGCTCGGc                                                                                                                        >  8:451512/1‑135 (MQ=255)               cAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCCCCCCCCCCACCCCCCCGGCGGCCCTGCTCGGc                                                                                                                        >  3:131893/1‑135 (MQ=255)                   ccAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCagag                                                                                                                    <  3:129317/135‑1 (MQ=255)                   ccAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCCGCCACCCCGC‑GCG‑CCCCACCCCCACCACCCCCCACGCCGCCAAGCACCGCagag                                                                                                                    >  3:22459/1‑135 (MQ=255)                    cAATACTGCAGCCCATGCCGATATCAACAACACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCAGCAGCCCCCGGGC‑GCT‑CCCCCCCCCCCCCCCCGCCCCAGCCGCCCTGGTCGGCaacca                                                                                                                   >  5:325354/1‑131 (MQ=255)                    cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCCCCCCCCACACCGCCCCCGCGGCCCAGCTCGCCAgcgc                                                                                                                   >  4:353699/1‑135 (MQ=255)                    cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACACGCAACCGCCCACCTGCCTTCAGCCACCCGGC‑GCG‑CCCCCCACACAACCCCCCCCCAGCGGCCCAGCACCGCAGAGc                                                                                                                   >  5:38547/1‑135 (MQ=255)                     aaTACTGAAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTGGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCg                                                                                                                  <  1:190672/135‑1 (MQ=255)                      aTACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGa                                                                                                                 <  2:19613/135‑1 (MQ=255)                             aGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCCCCCCCCACACCCCCCAGGCGGCCATGATCGGCAGAGCGATCAg                                                                                                             >  8:206030/1‑132 (MQ=255)                             aGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCG‑CCCCCCCCCCACCCCCCCCCCGGCGGCCCTGCTCGGCAGCGCcagcag                                                                                                             >  7:230834/1‑132 (MQ=255)                             aGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCAGCACCCACCCGGC‑GCG‑CCCCCCCCCCAACACCCCCCCAGCGGCCATGCACGGCAGAGCCCTCAg                                                                                                             >  2:190672/1‑132 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACCCTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCTCCCCCCCCCCCAACA‑CGCCCACCCCGCCAGGCTCGGCGGCGCGATCCGccac                                                                                                         >  6:62605/1‑131 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCCGCCCCCCGGC‑GCG‑CCCCCCCCCCCACCCCCCCCCCGCGGCCCAGCTCGGCAGCGCGCTCAGCCAgc                                                                                                        >  6:411084/1‑134 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCCGAGCGAGCAGCAAGa                                                                                                        >  6:101780/1‑135 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCG‑GCCCCCCCCCCACACCCCCCCGGCGGCCATGCTCGGCAGAGCGATCAGCaaca                                                                                                        >  1:383465/1‑133 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCCCCGGGC‑GCG‑GCCCACCCCCACCACCCCCCCGGCCGCCCTCCACGGCAGAGCGCTAAGCAAGa                                                                                                        >  1:293086/1‑135 (MQ=255)                               cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCACCGGGC‑GCG‑CCCCCCCCCCCACACCCCCCCCGCGGCCAAGCTCGGCAGCGCGCTCAGCAAGa                                                                                                        >  6:27584/1‑135 (MQ=255)                                gcttGCCGATATCAACAAACCCCCCGCCAACTCTTGGTTGTAAACCCGCAACCGCCCCGGTGCCTGCAGCGACCGGGC‑GCT‑CCCCACCGCCAACACCGCGCTGGCGGCCTTGCTCGGCTTAGCGATCAGCAAGaa                                                                                                       <  6:126342/132‑1 (MQ=255)                                 cATGCCGATATCAACAAAAACCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCACCCACCCCGC‑GCT‑GCCCCCCCCCAAACCCGCCCAGGCGGCCAAGCACCGCAGAGCGAAGAGCAAGAac                                                                                                      >  6:198523/1‑135 (MQ=255)                                  aTGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACCCCCGCCACCCCCCCCGTGCCGGCAGCCCCCCGGC‑GCG‑CCCCCCCCCCCACACCCCCCCCGCGGCCCAGCTCCGCAGCGCGCGCAGCAacaaca                                                                                                     >  8:56997/1‑135 (MQ=255)                                    gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGC‑GCG‑CCCCCCCCCCCACCCCCCCCCCGCCGCCCTGCTCGGCAGAGCGCTCCGCCAGAACAcc                                                                                                   >  6:318943/1‑135 (MQ=255)                                    gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCG‑CCCCACCCCCCACCCCGCCCAGGCGGCCAAGCTCGGCAGAGCGCTCAGCAAGAACAcc                                                                                                   >  3:356126/1‑135 (MQ=255)                                    gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGAGCCTGCAGCCACCCGGC‑GCT‑CCCCACCCCCAACACCCCCCAGGCGGCCATGCTCGGCAGAGCGCTAAGCAAGAACAcc                                                                                                   >  4:337800/1‑135 (MQ=255)                                      cGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCTTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCa                                                                                                  <  4:416560/134‑1 (MQ=255)                                       gATATCAACAAACCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGGGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAgc                                                                                                <  8:72008/135‑1 (MQ=255)                                       gATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCCTGCCAGCACCCCCCGGGC‑CCG‑CCCCCCCCCCCCCACCCCCCACGCCGCCCAGCTCCGCAGCGCCAACAGCCAGAACACCCgc                                                                                                >  8:187400/1‑135 (MQ=255)                                                 aaaCCCCCGCCAACACTTGGTTGACCACACGCACCCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGcc                                                                                      <  5:62605/135‑1 (MQ=255)                                                   aCCCCCGCCAACACTTGGTTGAACACCCGCAACGGCCCGGGTGCGTGCAGCCACGGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCcac                                                                                    <  5:404103/135‑1 (MQ=255)                                                      cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACt                                                                                 <  7:56997/135‑1 (MQ=255)                                                           cAACACTTGGTTGAACACCCGCAACCGCCACGGTTCCAGCAGCCACCCGGC‑CCT‑CACCACCCCCCAAACCCCCCCCGCCGCCATGCACCGCAGACGGATCAGGAACAACACCCCCGCCCGCCGGCCCACGGGcca                                                                            >  2:283982/1‑133 (MQ=255)                                                                         acacCCGCAACCGCCCCGGTGCCTGCCGCCACCGGGT‑GCT‑CCCCCCCCCCAACACCGCCCCGGCGGCCATGCTCGGCAGAGCGAGCAGCAAGAACACCAGCGCCAGCTGCCCCACTGGCAACGCCGGCGCGGCa                                                               >  4:90345/1‑134 (MQ=255)                                                                           acCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCCCCCCCCACACCGCCCAGGCGGCCATGCTCGGCAGCGCGCTCAGCAAGAACACCAGCGCCCGCTGCCACCCTGGCAACGACGGCGCGGCCAAt                                                            >  1:458272/1‑135 (MQ=255)                                                                                  aCCGCCCCGGTGCCTGCCGCCCCCGGGC‑GCG‑CCCCCCCCCCCCCCCCCCCCCGGCGGCCCAGCTCGGCCGCGCGCGCAGCAAGAACACCCCCCCCAGCGGCCCCCCAGGCACCGACCGCCCGGCCAATAcccccc                                                     >  5:250582/1‑133 (MQ=255)                                                                                     gCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACCCCCCCCAGCCGGCCATGCTCGGCAGAGCGAACAGCAACAACACCAGCGCCAGCAGCCACACAGGCAACCACGGCGCGGCAAAAACCCCGaccc                                                  >  1:334284/1‑134 (MQ=255)                                                                                         cGGGGCCGGCCGCCCCGGGGC‑GCT‑CCCCCCCCCCAACACCGCCCTGGCGGCCATGCTCGGCACAGCTATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGc                                              <  1:108398/135‑1 (MQ=255)                                                                                          ggTGCCTGCAGCCACCGGGC‑GCT‑GCCCACCCCCAACACCGCCCCGGCGGCCAAGCTCGGCAGAGCGCGCCGCAAGAACACCACCGCCAGCTGCCCCACTGGCAACGCCCGCGCGGCAAATAACCCGACCACcccc                                             >  2:445215/1‑135 (MQ=255)                                                                                               cTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACCCCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGc                                        <  1:103228/135‑1 (MQ=255)                                                                                                   aGCCACCGGGC‑GCT‑CCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCAt                                     >  4:203642/1‑134 (MQ=255)                                                                                                   aGCCACCGGGC‑GCT‑CCCCACCCCCCCCACCGCCCCGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCCCACTGGCAACGACGGCGCGGCACATACCCCGCCCACCGCCCCGGCCATc                                    >  3:13976/1‑135 (MQ=255)                                                                                                   aGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATc                                    <  7:192104/135‑1 (MQ=255)                                                                                                    gCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCCGGCGGCCATGCTCGGCAGAGCGATCAGCAAGACCACCAGCGCCAGCTGCCACACTGGCACCGCCGGCGCGGCAAATACCCCGCCCACCGCCACGGCCCTCa                                   >  1:382789/1‑135 (MQ=255)                                                                                                    gCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                                   <  3:353699/135‑1 (MQ=255)                                                                                                     cccccGGGCGGCC‑CCCCCCCCCCACCACCCCCCCGGCGGCCTTGCTCGCCAGAGCTAACACCACCACCACCCGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                                   <  5:388100/132‑1 (MQ=255)                                                                                                     ccACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGTCAAATACCCCGACCACCGCCACGGCCATCaa                                  <  7:355079/135‑1 (MQ=255)                                                                                                           ggC‑GCT‑CCCTACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcccc                                                       <  1:248029/108‑1 (MQ=255)                                                                                                           ggC‑GCT‑CCCTACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcccc                                                       >  2:248029/1‑108 (MQ=255)                                                                                                           ggC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGAc                            <  4:22459/135‑1 (MQ=255)                                                                                                            gc‑gcT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGAcc                           <  2:383465/135‑1 (MQ=255)                                                                                                                    ccccccccCACCCCCGCCCCGGCGGCCATGCTCGGCAGAGCGATCACCAAGACCACCCGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgggg                     <  2:458272/135‑1 (MQ=255)                                                                                                                       aCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAac                                                                                                      <  2:275888/51‑1 (MQ=255)                                                                                                                       aCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAac                                                                                                      >  1:275888/1‑51 (MQ=255)                                                                                                                               cacCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTg          <  4:131893/135‑1 (MQ=255)                                                                                                                                 ccGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCCCCACCTGCa        >  3:416406/1‑135 (MQ=255)                                                                                                                                     ccAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCCCCTGCAACGa    >  6:123839/1‑135 (MQ=255)                                                                                                                                     ccAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGa    <  7:303802/135‑1 (MQ=255)                                                                                                                                     ccAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGa    >  6:136821/1‑135 (MQ=255)                                                                                                                                     ccAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGa    >  3:83868/1‑135 (MQ=255)                                                                                                                                      cAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAg   >  2:445933/1‑135 (MQ=255)                                                                                                                                      cAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAg   >  2:195726/1‑135 (MQ=255)                                                                                                                                       aGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAgg  >  6:216204/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                       GTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGC‑GCT‑CCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGG  >  ACB122/2769964‑2770230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑