| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,856,886 | T→G | 100% | S297A (TCG→GCG) | hdfR_4 → | HTH‑type transcriptional regulator HdfR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,856,886 | 0 | T | G | 89.7% | 10.6 / ‑5.6 | 16 | S297A (TCG→GCG) | hdfR_4 | HTH‑type transcriptional regulator HdfR |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (1/12); total (1/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTG > ACB122/2856758‑2857017 | aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCGGCGCGcccg > 8:385453/1‑133 (MQ=255) ggggtgCTGCCCGGGGGGGGGCACCGGTTTTCGCACACCACCCTCCCGCTCAGGGGCGGCCCCCGGGGGGGGTGGGGGTGCCCGCGGGCGGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCcgcg < 6:315514/133‑1 (MQ=255) ggccccGGCGGGGGACCCGGCTGTGCCACCATCCCCTGCCGGTCACGGGCGCGCCCCAGCCGCGGGGGGTGGTGCGGCGGCCCGGGGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGa < 4:49811/132‑1 (MQ=255) ggccccccGCTGTCCCACCCTCCCCCGCCGCCCTCGGGCGGCCACCCGGCGGGGGGGGGGTGGCCGGGGGCCGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTgg < 1:384577/130‑1 (MQ=255) ggCCCCCGCTGTTCCCCACACCCCGCCCGCTCACGGGCGGCCACCCGGCGCGCGGGGGGTGGCCGGGGGCCGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGc < 2:307383/135‑1 (MQ=255) cccacaCTCCCCGCACGCTCCGGGGCGGGCACCAGGCGCGGGGGGTGGGGCCGGGGGCGGGCTCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGaaa < 7:191648/133‑1 (MQ=255) ccTGACGCTCACGGGCGTGCACCAGGCGCGCGGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGAt < 7:338122/133‑1 (MQ=255) gcccgcgCACGGGCGTGCACCAGGCGCGGGGGGGGTTGCGGCGGGCGGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTgg < 8:106240/132‑1 (MQ=255) gcccatgggcgggcACCAGGCGCGGGGGGTGTTGCCGGGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGt < 3:444057/129‑1 (MQ=255) cccgggCGCGCACCCGGCGCGGGGGGTGGGGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 2:141243/132‑1 (MQ=255) cgggCGGCCACCCGGCGCGGGGGGGGTGCCCGCGGGCGGGCCCGCGGGCGCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 7:278466/132‑1 (MQ=255) aGGGCGTGCACTTGGGGCGCGGGGTGTGGCGGGGGGCGGGGTCGCCGGCCCTTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGTACTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATcc < 1:61588/135‑1 (MQ=255) tggCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATg < 1:89554/133‑1 (MQ=255) gcccGGCGCGCCGGCCCCTCAGCTGCGCCACAACTGCAGCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTggcggc < 2:110825/133‑1 (MQ=255) cgggCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGcggcgg < 3:425866/133‑1 (MQ=255) ggCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGgtg < 3:282989/135‑1 (MQ=255) | ACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTG > ACB122/2856758‑2857017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |