Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,204,513 G→C 29.4% E165Q (GAA→CAA)  CJ019_03009 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,204,5130GC29.4% 67.9 / 4.3 42E165Q (GAA→CAA) CJ019_03009hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/4);  new base C (2/5);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 7.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCTCAGAGGCACCA  >  ACB122/3204386‑3204643
                                                                                                                               |                                                                                                                                  
gATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAg                                                                                                                             >  7:117054/1‑135 (MQ=255)
gATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAg                                                                                                                             >  6:169089/1‑135 (MQ=255)
gATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAg                                                                                                                             >  4:199817/1‑135 (MQ=255)
gATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGAGCTCGAGGAAATCCTGGAGGTCTCGTCGCGGTCGATCCCGGAATTCATCGACAGCCACATGCCAGTGGTGCCGCAGCTCATCCTCCAAGAAAAAAg                                                                                                                             >  3:262357/1‑135 (MQ=255)
 atgatgTTGGCTTTCAGCCGGACGCAGGAGCCCGGGGTGCTCGAGTAATTCCTGTAGGGCTGGTCGGGGTCTATCGCGGAATTCATCTACAGAAACATGGGCGTGGTGACGCAGATCATCCTCGAACAAAAAAgc                                                                                                                            <  2:6024/135‑1 (MQ=255)
         ggCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGc                                                                                                                    >  8:115377/1‑135 (MQ=255)
           ccTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGccc                                                                                                                  >  7:294550/1‑135 (MQ=255)
           ccTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGccc                                                                                                                  >  3:138120/1‑135 (MQ=255)
           ccTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTAGGAATTCATAGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGccc                                                                                                                  >  3:129948/1‑135 (MQ=255)
                  ccTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATGTCGGCATTCATCGACAGCAACATGCGCGTGGTGACGCAGAAGATCCTCGAACAAAAAAGCGCCCACGCCCATgacga                                                                                                           >  8:1419/1‑135 (MQ=255)
                       cGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCAATGACGACCCTa                                                                                                      >  4:55361/1‑135 (MQ=255)
                        gCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTAt                                                                                                     >  8:323181/1‑135 (MQ=255)
                        gCAGGATCCCGCGGGGCTCGAGGATTTCCTGGAGGTATCGTCGCGGTGTATCTCGTAATTCATCGACAGAAAAATGCGCGTGGTGACGCAGATAATCCTCTAATAAAAAAGCGCCCAGGCCCATGACGACCCTAt                                                                                                     <  8:82497/135‑1 (MQ=255)
                         cAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGGCAGCAACATGCGCGTGGTGCCGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTAt                                                                                                     >  8:247736/1‑134 (MQ=255)
                         cAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCCACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCAAGACGACCCTAt                                                                                                     >  6:396225/1‑134 (MQ=255)
                               cccGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTGGATCTCGGCATTCATCGACAGCAACCTGCGCGTGGTGACGCCGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGCCCCTATCGATaaa                                                                                              >  1:244365/1‑135 (MQ=255)
                               cccGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATaaa                                                                                              >  2:251042/1‑135 (MQ=255)
                                   cgGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCa                                                                                          >  4:431389/1‑135 (MQ=255)
                                    ggTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGccc                                                                                                                        >  8:111096/1‑104 (MQ=255)
                                    ggTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGccc                                                                                                                        <  7:111096/104‑1 (MQ=255)
                                         tCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGCTCTCGGAATTAATCGACAGCAACCAGCGCGTGGTGGCGCAGATCATCCTCGAACAAAAAAGCCCCCCGGCCCATGACGCCCCGATCGCTAAACGCCGCCggg                                                                                    >  6:65097/1‑135 (MQ=255)
                                         tCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGCCCCTATCGATAAACGCAGCCTgg                                                                                    >  8:132583/1‑135 (MQ=255)
                                              caaTTCCTGGCGGGCGCGGCGCGGGCGATCTCGAAATTCATCGACAGCAACATGCGCGGGGTCACGCTCATCAGCCTCCAACAAAAACGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGc                                                                               <  4:333004/134‑1 (MQ=255)
                                                          gtcgcgtcgcGGTCGATCTCGTATTTCATCGACAGCAACATGCGCGTGGTGACGCATATCATCCGCGAACAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGAt                                                                   <  4:138120/135‑1 (MQ=255)
                                                             ggggcgcgGGCTACCTCGTAATTCAGCCACAGAAACAGGCGCGGGGGCACCCAGAGCACCCTCCAAAAAAAACGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGc                                                                <  4:326721/131‑1 (MQ=255)
                                                                  gcgGGCGACCTCGCAATACACCCACACCAACTGGCGGGGGGGGCCGCATATCACCCCCCAAAAAAACCGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGa                                                           <  7:132583/135‑1 (MQ=255)
                                                                       ctacctcGTATTTAATCGACAGAAACATGCGGGGGGTGACGAATATAATCCTCCAAAAAAAACGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTgg                                                      <  4:384128/131‑1 (MQ=255)
                                                                       cGAGCTCGTAATTCATCCACAGCAACAGGCGCGTGGTGACGCAGATCAGCGTCGAATAAAAACGCGCCCAGGCCCATGACGAACCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTgg                                                      <  3:98150/135‑1 (MQ=255)
                                                                            tgggtaTTCATCGACTGCTAGAGGCGGGTGGTCCCGCATACCAGCCTGCAACAAAAACGCCCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGAcgcg                                                 <  6:185030/130‑1 (MQ=255)
                                                                                 aTTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCa                                            >  2:328041/1‑135 (MQ=255)
                                                                                   tCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGt                                          >  6:229895/1‑135 (MQ=255)
                                                                                           accAACATGCGCGTGGTCAAGCACATCATCCTCTAAAAAAAACCCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAgg                                  <  7:277254/133‑1 (MQ=255)
                                                                                            gaaaCTTGCGGGTGGTCCCGCAGATCACCCTCTAAAAAAAACGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGc                                 <  3:431389/133‑1 (MQ=255)
                                                                                             cAACAGGCGCGGGGGCCCCCAGATCACCCTCCAACAAAAACGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCg                                <  8:37908/135‑1 (MQ=255)
                                                                                                     gcgTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCt                        >  3:442939/1‑135 (MQ=255)
                                                                                                               gCAGAGCATCCTCGACGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGc              <  2:104765/135‑1 (MQ=255)
                                                                                                                cAGATCACACGCTAAAAAAACCGCGCCCCGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCt             <  4:8401/135‑1 (MQ=255)
                                                                                                                 gcatcaccCGCGAAGAACAACGCGCCCAGGCCCTTGACGACCCTATCGATACCCGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGAGGTGGACGCGGAGCAGGTCGGCAGGCGACGGGGCTACAGCCTGGCTc            <  2:280599/133‑1 (MQ=255)
                                                                                                                 aGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGc              >  2:367442/1‑133 (MQ=255)
                                                                                                                     catcCTCGAAGAAAACCGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCTCagag        <  2:35495/135‑1 (MQ=255)
                                                                                                                          tCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCTCAGAGGCAcc   >  1:286898/1‑135 (MQ=255)
                                                                                                                           cGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCTCAGAGGCACCa  >  1:180432/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                                  
GATGAAGATGGCCTTCAGCCTGACGCAGGATCCCGCGGTGCTCGAGGAATTCCTGGAGGTCTCGTCGCGGTCGATCTCGGAATTCATCGACAGCAACATGCGCGTGGTGACGCAGATCATCCTCGAAGAAAAAAGCGCCCAGGCCCATGACGACCCTATCGATAAACGCAGCCTGGTCAGCCGGCTGCTCGATGGCAGGGATGTGGACGCGGAGCAGTTCGGCAGGCGACTGGGCTACAGCCTGGCTCAGAGGCACCA  >  ACB122/3204386‑3204643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑