Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,218,100	C→T	6.8%	A126T (GCG→ACG)	nodD2_2 ←	Nodulation protein D 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,218,100	0	C	T	6.8%	194.4 / 3.2	59	A126T (GCG→ACG)	nodD2_2	Nodulation protein D 2
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base T (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CGCAGCACCTTGCGCTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGACTGCAGTGGAAATCGAA  >  ACB122/3217967‑3218233                                                                                                                                      |                                                                                                                                      cgcAGCACCTTGCGCTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCTTCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGcc                                                                                                                                      >  3:187827/1‑135 (MQ=255)   cagcaCCTTGCGCTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTc                                                                                                                                    <  7:395146/135‑1 (MQ=255)   cagcaCCTTGCGCTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTc                                                                                                                                    <  6:78067/135‑1 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCcgcgcggacgg                                                                                                                         >  1:460382/1‑130 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATgc                                                                                                                         >  8:40422/1‑135 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATgc                                                                                                                         >  8:183956/1‑135 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATgc                                                                                                                         >  4:447628/1‑135 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAAAgc                                                                                                                         >  3:271470/1‑135 (MQ=255)              gcTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCCCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCACGCcgcgcaagc                                                                                                                         >  6:316039/1‑130 (MQ=255)                   gCGTTGGCCGGCAGTGCGCTGGTGGAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTg                                                                                                                    <  1:214705/135‑1 (MQ=255)                    cGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTg                                                                                                                    <  8:203651/134‑1 (MQ=255)                     gTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGa                                                                                                                   >  2:105556/1‑134 (MQ=255)                     gTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGa                                                                                                                   >  2:42831/1‑134 (MQ=255)                           cGGCAGATCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGCGCAGTTg                                                                                                            >  5:67709/1‑135 (MQ=255)                               aGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGgc                                                                                                        <  7:183956/135‑1 (MQ=255)                                 ttCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGgca                                                                                                       >  3:225610/1‑134 (MQ=255)                                      tGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAgg                                                                                                 >  1:349370/1‑135 (MQ=255)                                              cTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCg                                                                                         >  3:227733/1‑135 (MQ=255)                                                 aCCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGcg                                                                                      >  5:126605/1‑135 (MQ=255)                                                  cccccACCGAACTCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCCCCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGc                                                                                       <  3:330403/133‑1 (MQ=255)                                                     ccACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGcgtcgt                                                                                  >  4:444907/1‑135 (MQ=255)                                                      cACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATCCCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCgtcgtc                                                                                 >  5:119119/1‑135 (MQ=255)                                                      cACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCgtcgtc                                                                                 >  7:288611/1‑135 (MQ=255)                                                      cACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCgtcgtc                                                                                 >  8:247308/1‑135 (MQ=255)                                                      cACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCgtcgtc                                                                                 <  4:367063/135‑1 (MQ=255)                                                          gAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGTCTCGgcgc                                                                                                                               >  6:463391/1‑85 (MQ=255)                                                          gAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGTCTCGgcgc                                                                                                                               >  6:457175/1‑85 (MQ=255)                                                          gAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGTCTCGgcgc                                                                                                                               <  5:463391/85‑1 (MQ=255)                                                          gAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGTCTCGgcgc                                                                                                                               <  5:457175/85‑1 (MQ=255)                                                          gAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCg                                                                               >  6:433890/1‑133 (MQ=255)                                                           acaTCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCCCCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACa                                                                            <  5:56832/133‑1 (MQ=255)                                                           aaaTCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACa                                                                            <  7:189088/135‑1 (MQ=255)                                                           aaaTCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACa                                                                            >  3:159278/1‑135 (MQ=255)                                                           aaaTCTCGCCCGAGGCCAGCAGGGGTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACa                                                                            <  3:295119/135‑1 (MQ=255)                                                             aTCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCCGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAgg                                                                          <  1:297495/135‑1 (MQ=255)                                                                     cGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCg                                                                  <  3:166223/135‑1 (MQ=255)                                                                        ggCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAACACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGaa                                                               <  2:34040/135‑1 (MQ=255)                                                                            agcagGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGTCCGATGCGGAACAcc                                                           <  7:430446/135‑1 (MQ=255)                                                                            agcagGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACAcc                                                           <  2:349370/135‑1 (MQ=255)                                                                            agcagGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACAcc                                                           <  1:129897/135‑1 (MQ=255)                                                                               agGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCa                                                        >  8:71551/1‑135 (MQ=255)                                                                                      gCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGCCAGGCCGATGCGGAACACCGCATTGCtgg                                                 >  7:304234/1‑135 (MQ=255)                                                                                       cATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCtggt                                                <  3:217887/135‑1 (MQ=255)                                                                                        aTCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCtggtg                                               <  6:28022/135‑1 (MQ=255)                                                                                              aaGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCggg                                         >  8:412104/1‑135 (MQ=255)                                                                                                        cccGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcgg                               >  3:457928/1‑135 (MQ=255)                                                                                                         ccGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGCATGCGCTTGAGCAGTTGCGGCCGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACCCCGCCTTGCGGGGGGCCGGGTCGAATTcggc                              >  4:104047/1‑135 (MQ=255)                                                                                                         ccGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggc                              >  8:9923/1‑135 (MQ=255)                                                                                                          cGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             <  6:126605/135‑1 (MQ=255)                                                                                                          cGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             <  3:227128/135‑1 (MQ=255)                                                                                                          cGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGAGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             <  4:242063/135‑1 (MQ=255)                                                                                                          cGTCCCTCCCCCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTGGAGCAGTTGCGGCAGGAAGGCGAACTCGGCGTCGGCCGACAGTCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             <  5:316039/135‑1 (MQ=255)                                                                                                               caccacCAGCACGATACCGGGCGCCTCGGCGCGAATGCGGTTGAGGAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGgcggcgcg                        <  4:252197/135‑1 (MQ=255)                                                                                                               caccacCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGgcggcgcg                        <  3:447628/135‑1 (MQ=255)                                                                                                                      aGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGCCAGGCCGATGCGGAACAACGCATAGCTGGTGGCCGGGGCGCATTCGGCGGCGCGGCTGAcg                 >  5:105454/1‑134 (MQ=255)                                                                                                                          cGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGACTGCAg             <  6:143776/135‑1 (MQ=255)                                                                                                                               ccGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGACTGCAGTGGaa        >  8:151330/1‑135 (MQ=255)                                                                                                                                cGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGTGTCGAATTCGGCGGCGCGGCTGACTGCAGTGGaa        <  4:190502/134‑1 (MQ=255)                                                                                                                                cGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             <  4:36699/113‑1 (MQ=255)                                                                                                                                cGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTcggcg                             >  3:36699/1‑113 (MQ=255)                                                                                                                                    cgcCTCGGCGCGAATGCGCTTGAGCAGTAGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATAGCTGGTGGCCGGGTCGATTTCGGCGGCGCGGCTGACTGCAGTGGAAATCGa   >  7:151456/1‑135 (MQ=255)                                                                                                                                     gcCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGACCACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGCCTGCAGGGGAAATCGaa  >  7:409840/1‑135 (MQ=255)                                                                                                                                     gcCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACAcc                                                           >  3:339698/1‑78 (MQ=255)                                                                                                                                     gcCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACAcc                                                           <  4:339698/78‑1 (MQ=255)                                                                                                                                     gcCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGACTGCAGTGGAAATCGaa  >  2:54890/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCAGCACCTTGCGCTTGGCGTTGGCCGGCAGTTCGCTGGTGTAGCTGACCCCCACCGAAATCTCGCCCGAGGCCAGCAGGGTTGGCATCAGCAAGTAGTTGACCCGTCGCACCACCAGCACGATACCGGGCGCCTCGGCGCGAATGCGCTTGAGCAGTTGCGGCAGCAAGGCGAACTCGGCGTCGTCCGACAGGCCGATGCGGAACACCGCATTGCTGGTGGCCGGGTCGAATTCGGCGGCGCGGCTGACTGCAGTGGAAATCGAA  >  ACB122/3217967‑3218233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑