Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,238,596 C→A 6.2% intergenic (‑45/‑171) CJ019_03036 ← / → xerC_2 hypothetical protein/Tyrosine recombinase XerC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,238,5960CA 6.2% 184.8 / 3.5 65intergenic (‑45/‑171)CJ019_03036/xerC_2hypothetical protein/Tyrosine recombinase XerC
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base A (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

AACTGCCACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCTG  >  ACB122/3238470‑3238727
                                                                                                                              |                                                                                                                                   
aacTGCCACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCg                                                                                                                             >  7:334009/1‑135 (MQ=255)
 acTGCCACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGt                                                                                                                            <  8:250546/135‑1 (MQ=255)
    gCCACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTg                                                                                                                           >  8:422755/1‑133 (MQ=255)
     ccACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCa                                                                                                                        >  6:410604/1‑135 (MQ=255)
     ccACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCa                                                                                                                        >  6:210513/1‑135 (MQ=255)
        cccAGGAAGAACGGGCGAAAACTCCGGCTCCAGACGGCTTGGCGTTGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGtt                                                                                                                     <  1:197311/135‑1 (MQ=255)
        cccAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGtt                                                                                                                     <  6:407851/135‑1 (MQ=255)
                gaaCGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGcc                                                                                                             <  8:372690/135‑1 (MQ=255)
                gaaCGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGcc                                                                                                             <  7:422755/135‑1 (MQ=255)
                gaaCGGGCGAAAACTCAGGCTCCAGACGGCGTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGcc                                                                                                             <  8:109050/135‑1 (MQ=255)
                 aaCGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGccc                                                                                                            <  8:262122/135‑1 (MQ=255)
                  aCGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCt                                                                                                           <  2:53710/135‑1 (MQ=255)
                      gCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGa                                                                                                        >  2:448210/1‑134 (MQ=255)
                      gCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCACTTGa                                                                                                        >  6:21642/1‑134 (MQ=255)
                        gAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATAACGATCCGTGCCAGTTGCAAAGCCCTTGAata                                                                                                     >  5:33894/1‑135 (MQ=255)
                        gAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATAACGATCCGTGCCAGTTGCAAAGCCCTTGAat                                                                                                      >  3:385485/1‑134 (MQ=255)
                         aaaaCTCCGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGCTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAatat                                                                                                    <  5:354752/135‑1 (MQ=255)
                         aaaaCTCATGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAatat                                                                                                    <  7:463037/135‑1 (MQ=255)
                                 ggCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTg                                                                                             <  8:82870/134‑1 (MQ=255)
                                    tCCCGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGTGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATgg                                                                                         <  8:409486/135‑1 (MQ=255)
                                        gACGGCGTGGCGGGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACt                                                                                     <  1:148706/135‑1 (MQ=255)
                                         aCGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACtt                                                                                    <  7:225534/135‑1 (MQ=255)
                                                ggcgtgggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAggg                                                                             >  1:415385/1‑135 (MQ=255)
                                                 gcgtgggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAgg                                                                                               <  1:70153/116‑1 (MQ=255)
                                                 gcgtgggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAgg                                                                                               >  2:70153/1‑116 (MQ=255)
                                                    tgggcgtgTAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCg                                                                         <  5:410604/135‑1 (MQ=255)
                                                    tgggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATAACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCg                                                                         <  4:385485/135‑1 (MQ=255)
                                                    tgggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATAACGATCCGTGCCAGTTGCAAAGCCATTGAATATCAAGGTGATGGCACTTATCAGGGTCCg                                                                         <  6:33894/135‑1 (MQ=255)
                                                      ggcgtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGaa                                                                       <  1:448210/135‑1 (MQ=255)
                                                         gtgAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATg                                                                     >  7:317880/1‑134 (MQ=255)
                                                           gAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGaca                                                                  <  8:317880/135‑1 (MQ=255)
                                                                     gTTGCAAGAGCATGGGGAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGtt                                                        <  5:382272/135‑1 (MQ=255)
                                                                     gTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGtt                                                        >  3:440540/1‑135 (MQ=255)
                                                                     gTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGtt                                                        <  8:334009/135‑1 (MQ=255)
                                                                     gTTGCAAGAGCATGGGCAGTCCTTGGTTAGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGtt                                                        <  2:285662/135‑1 (MQ=255)
                                                                     gTTGCAAGAGCATGGGCAGTCCTTGGTGCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGtt                                                        <  5:451870/135‑1 (MQ=255)
                                                                          aaGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCgg                                                    >  1:38722/1‑134 (MQ=255)
                                                                          aaGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCgg                                                    >  4:130638/1‑134 (MQ=255)
                                                                          aaGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCgg                                                    >  1:299157/1‑134 (MQ=255)
                                                                                          cTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCtt                                    >  5:174249/1‑134 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTc                                              >  1:286505/1‑123 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTc                                              <  2:286505/123‑1 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTg                                   <  3:224244/134‑1 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTg                                   <  2:299157/134‑1 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGt                                  >  3:443961/1‑135 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGt                                  >  7:276539/1‑135 (MQ=255)
                                                                                           ttGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGt                                  >  7:138654/1‑135 (MQ=255)
                                                                                           ttGGTGCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGt                                  <  5:159923/135‑1 (MQ=255)
                                                                                                     cAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGtt                                                                                                                     >  5:257962/1‑42 (MQ=255)
                                                                                                     cAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGtt                                                                                                                     <  6:257962/42‑1 (MQ=255)
                                                                                                     cAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGttt                         >  8:12014/1‑134 (MQ=255)
                                                                                                      aGTATGCTTGAATTGGTGTACGATCACGAGCCGTGCCAGTTGCAAAGCCGTTGAATATCCAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTg                       <  1:304076/135‑1 (MQ=255)
                                                                                                      aGTAGGCTGCAATTGGGGTAAGATCCCGATCCGTGCCAGTTGCACAGCCCGTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGGCGTTTTg                       <  6:7563/135‑1 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGt                                                                            <  4:443095/72‑1 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGt                                                                            >  3:443095/1‑72 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCa             >  7:39736/1‑135 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCa             >  4:97385/1‑135 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCa             >  6:302202/1‑135 (MQ=255)
                                                                                                                aaTTGGGGTAAGATCACGATACGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGCCACTTATCAGGGTCAGAATGACAAGCGTGTGTTGCTGTCATTAAGACTGGCTATTATGTCGTTTTGAGTTGCTTCa             >  1:309924/1‑135 (MQ=255)
                                                                                                                 aTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAg            <  6:341121/135‑1 (MQ=255)
                                                                                                                 aTTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAg            <  8:358643/135‑1 (MQ=255)
                                                                                                                          aGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCt   >  3:339078/1‑135 (MQ=255)
                                                                                                                          aGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCt   >  2:284135/1‑135 (MQ=255)
                                                                                                                          aGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCt   >  3:233439/1‑135 (MQ=255)
                                                                                                                           gATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCTg  <  1:117175/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                   
AACTGCCACCCAGGAAGAACGGGCGAAAACTCAGGCTCCAGACGGCTTGGCGTGGGCGTGAAGCAGTGGGTTGCAAGAGCATGGGCAGTCCTTGGTTCGTTCAGTAGGCTTCAATTGGGGTAAGATCACGATCCGTGCCAGTTGCAAAGCCCTTGAATATCAAGGTGATGGCACTTATCAGGGTCCGAATGACACGCGTGTGTTGCGGTCATTCAGACTGGCTTGTATGTCGTTTTGACTTGCTTCAGGGTTTGCCTG  >  ACB122/3238470‑3238727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑