| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,255,897 | A→C | 28.4% | intergenic (‑152/+72) | pnuC ← / ← mcpP | Nicotinamide riboside transporter PnuC/Methyl‑accepting chemotaxis protein McpP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,255,897 | 0 | A | C | 28.4% | 22.6 / 4.0 | 36 | intergenic (‑152/+72) | pnuC/mcpP | Nicotinamide riboside transporter PnuC/Methyl‑accepting chemotaxis protein McpP |
| Reads supporting (aligned to +/- strand): ref base A (22/3); new base C (6/5); total (28/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GGGAAGGGGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCAATGCCACCC > ACB122/3255767‑3256030 | gggAAGGGGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGggc > 1:50014/1‑135 (MQ=255)gggAAGGGGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGggc > 7:7218/1‑135 (MQ=255) ggCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GACGGCAGGGCCGGccca > 7:429994/1‑134 (MQ=255) gCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCCGCCGGGCCGGCCC‑tt > 1:115657/1‑135 (MQ=255) cGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑t > 3:206457/1‑133 (MQ=255) agaTTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCAGGCCC‑ttt > 3:99183/1‑133 (MQ=255) gTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGACCC‑TTTC‑G‑CGGGt > 3:370362/1‑135 (MQ=255) cGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCCGGGGCGGCCC‑GTTC‑GCCGGGTGGA‑ccgct > 1:414278/1‑132 (MQ=21) cgcAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTA‑G‑CGGGTGCA‑CCC‑GCTCCTACGACGGCTTGTGCCgc > 8:330240/1‑134 (MQ=255) cTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGCA‑CCC‑GCTCCGACGACGGCTTGTGCCGGacccc > 7:302644/1‑133 (MQ=255) cTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGcaccac > 8:439075/1‑135 (MQ=255) cTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTC‑G‑CGGGTGAA‑CCC‑GCGCCGACGCCGGCGTGTGGCGCacccc > 7:28923/1‑133 (MQ=17) tCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGCaccacc > 6:226599/1‑135 (MQ=255) tttGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGCA‑CCC‑GCTCCTACGACGGCTTGTGCCGCACCCCCGATGgac > 1:39760/1‑135 (MQ=255) gTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTC‑G‑CGGGTGAA‑CCC‑GCGCCTACGGCGGCTTGTGGCGCCCCCCCGATGgacgcc > 5:454439/1‑133 (MQ=14) aGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCCGGGCCGGCCC‑GTTC‑G‑CGGGGGAG‑CCC‑GCTCCTACGTCGGCGTGTGCCGCACCACCGAGGgccgcc > 7:18697/1‑131 (MQ=14) aGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTC‑G‑CGGGTGGG‑CCC‑GCGCCTACGACGGGGTTTGGCGCACCACCGCTGgccggc > 8:310215/1‑131 (MQ=14) gggTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTGCTACGACGGCTTGTGCCGAACCCCCGCTGGCCGCCGTAGGc > 4:128749/1‑133 (MQ=17) ggTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCCGGGCCGGCCC‑TTTC‑G‑CGGGTGCA‑CCC‑GCGGCGTCGCCGGCTTGTGGCGAACCACCGATGGCCGACGTAGGCTCa > 6:67635/1‑135 (MQ=17) gTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGCA‑CCC‑GCTCCGACGCCGGGTTGTGCCGCACCACCGATGGCCGCCGGAGGGTCAg > 4:451559/1‑135 (MQ=17) gTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAACCACCGCTGGACGACGTAGGCTCAg > 8:26425/1‑135 (MQ=255) ttACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCCGGGCCGGCCC‑GTTC‑G‑CGGGTGGA‑CCC‑GCTGCGACGCCGGCGTGTGCCGGACCCCCGAGGGCCGGCGTAGGGGCAGAc > 1:55246/1‑135 (MQ=14) ttACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTA‑G‑CGGGTGAA‑CCC‑GGTCCGACGCCGGCTTGTGCCGCACCCCCGATGGACGGCGTAGGGTGAGAc > 7:461415/1‑135 (MQ=17) tACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑GTTG‑G‑CGGGGGGA‑CCC‑GCGCCTACGACGGGTTGTGCCGCACCCCCGCGGGGCGCCGTTGGCTCCGCCCCGGCACTGcc > 6:318545/1‑135 (MQ=17) cggcggCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCCGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAAACAGCGATGGACGACCTAGGCGCAGACCCCGAACTGCCGcacccac > 8:152810/1‑130 (MQ=21) cggcAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGcc > 8:464447/1‑83 (MQ=255) cggcAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGcc < 7:464447/83‑1 (MQ=255) aCGGGGCACGGGGGGGGGAAATTG‑GCCCGCCGGGGCGGGCC‑GTTCGG‑CGGGTCAC‑CCG‑GCCCCAACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCa < 7:439075/135‑1 (MQ=255) aCGGGGACCGGGGGGGGGAAAATG‑GCCGGCCGGGCCGGGCC‑GTTT‑G‑GGGGTTAACCCC‑GCGCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCa < 7:330240/135‑1 (MQ=255) ggcggGGGGAAATTG‑GCCGGCCGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGCCGGCTTGTGCCGCACCCCCGAAGGGCGACGTAGGCTCAGGCCCGGAACTGCCGCAACAACTGCTCCAGCGCTTAGca > 2:288356/1‑134 (MQ=25) gggggggcAATTTG‑GCCGCCCGGGCGGCCCC‑GTTC‑G‑CGGGTCAC‑CCG‑GCCCCAACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 3:338241/133‑1 (MQ=255) ggcgggggAAATTGCGCGGGCCGGGCGGGCCC‑TTGC‑G‑GGGGGGAA‑CCC‑GCCCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCt < 6:454439/132‑1 (MQ=255) gcggGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 1:288356/135‑1 (MQ=255) gggggggAAACTG‑GCCGGGCGGGCCGGCCC‑CTTG‑G‑GGGGGGCA‑CCCGGCTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 2:249372/134‑1 (MQ=255) gAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGcc > 8:395378/1‑135 (MQ=255) gg‑gccggccGGGGCGGCCCGTTGC‑G‑GGGGGAAC‑CCC‑GCCCCAACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCaa < 8:302644/134‑1 (MQ=255) cAGGGCCGTCCC‑TTTC‑C‑CGGGTGAA‑CCC‑GATCCTACGACGGCTTGTGCCGAACCACCTATGGACGACGTAGGCTCAGACCCGGAACTGCAGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCAATGCCAcca < 8:251244/135‑2 (MQ=255) | GGGAAGGGGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAATTG‑GCCGGCAGGGCCGGCCC‑TTTC‑G‑CGGGTGAA‑CCC‑GCTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCAATGCCACCC > ACB122/3255767‑3256030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |