Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	299,787	C→A	5.1%	H218Q (CAC→CAA)	hslO →	33 kDa chaperonin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	299,787	0	C	A	5.1%	256.5 / 3.7	78	H218Q (CAC→CAA)	hslO	33 kDa chaperonin
Reads supporting (aligned to +/- strand):  ref base C (46/27);  new base A (2/2);  total (48/30)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

AACTGCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTGGCGG  >  ACB122/299654‑299918                                                                                                                                      |                                                                                                                                    aaCTGCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACg                                                                                                                                    <  1:158615/135‑1 (MQ=255)  aCTGCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGa                                                                                                                                   <  4:64118/135‑1 (MQ=255)     gCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCAAGa                                                                                                                                   <  8:297478/132‑1 (MQ=255)     gCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCAAGa                                                                                                                                   <  8:290224/132‑1 (MQ=255)     gCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCAAGa                                                                                                                                   >  7:297478/1‑132 (MQ=255)     gCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCAAGa                                                                                                                                   >  7:290224/1‑132 (MQ=255)        aCGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGc                                                                                                                             >  2:172071/1‑135 (MQ=255)            gATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTg                                                                                                                         <  5:184492/135‑1 (MQ=255)                  cAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTg                                                                                                                   <  4:396210/135‑1 (MQ=255)                    gCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGtt                                                                                                                 <  2:12453/135‑1 (MQ=255)                                    cgcgAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTg                                                                                                 <  4:345578/135‑1 (MQ=255)                                             aGCTGGCAGCTTGTGGTCGCCCTGGCCAAGACACTCACCGCTTAAGAGTGGACAGAGGGCAACGAAACACTGGTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAAt                                                                                        <  5:420089/135‑1 (MQ=255)                                                  gcagcaTGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAg                                                                                   >  2:374836/1‑135 (MQ=255)                                                      caTGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGt                                                                               <  6:113310/135‑1 (MQ=255)                                                        tgtgGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTc                                                                             >  1:159546/1‑135 (MQ=255)                                                            gTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTcgcg                                                                          >  5:313857/1‑134 (MQ=255)                                                            gTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTcgcg                                                                          >  6:28295/1‑134 (MQ=255)                                                               gCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGaa                                                                      >  5:302844/1‑135 (MQ=255)                                                               gCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGaa                                                                      >  1:357808/1‑135 (MQ=255)                                                                      ccAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCg                                                               <  4:105855/135‑1 (MQ=255)                                                                        aaGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCgg                                                              >  3:378511/1‑134 (MQ=255)                                                                           acacTCACGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAAt                                                          <  6:384777/135‑1 (MQ=255)                                                                             acTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTgcgc                                                                                              <  3:135218/97‑1 (MQ=255)                                                                             acTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTgcgc                                                                                              >  4:135218/1‑97 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTTCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTgg                                                   >  5:8638/1‑135 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCAGGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCTGGAACGCTCCGGCAATGCGCt                                                     <  5:456753/133‑1 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCa                                                                                    <  1:341262/102‑1 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCa                                                                                    >  2:341262/1‑102 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCt                                                     <  7:138274/133‑1 (MQ=255)                                                                                  aGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTgg                                                   >  2:164573/1‑135 (MQ=255)                                                                                    ccTGAAGAGTGGACAGACGGCAACGAAACACTGCTGCACCGCCTGTATCACGACGACGCCGTGCGCCTGTTCGATATCCAGCCACTGAGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTc                                                 <  2:55947/135‑1 (MQ=255)                                                                                       gAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGt                                              >  6:125218/1‑135 (MQ=255)                                                                                          gagTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTg                                           >  3:7398/1‑135 (MQ=255)                                                                                           agTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTgg                                          >  7:39493/1‑135 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCTGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGa                                      >  7:431599/1‑134 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCt                                                     <  5:87240/119‑1 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCt                                                     >  6:87240/1‑119 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGa                                      >  7:258046/1‑134 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGa                                      >  1:262077/1‑134 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     <  6:313857/135‑1 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     >  4:24462/1‑135 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     >  7:197907/1‑135 (MQ=255)                                                                                                aCAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTGCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     >  6:28834/1‑135 (MQ=255)                                                                                                  agagGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCTGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCa                                   >  2:45743/1‑135 (MQ=255)                                                                                                  agagGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCa                                   >  2:74562/1‑135 (MQ=255)                                                                                                       gCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGata                                                                                                            >  4:285848/1‑57 (MQ=255)                                                                                                       gCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGata                                                                                                            <  3:285848/57‑1 (MQ=255)                                                                                                        cAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGCTATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGc                             >  5:113273/1‑135 (MQ=255)                                                                                                            gAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     >  7:144986/1‑123 (MQ=255)                                                                                                            gAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAg                                     <  8:144986/123‑1 (MQ=255)                                                                                                            gAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAg                         >  2:384819/1‑135 (MQ=255)                                                                                                            gAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAg                         >  1:119183/1‑135 (MQ=255)                                                                                                                 acTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGACCGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGCCGCCAAGGCGCt                    >  2:257823/1‑135 (MQ=255)                                                                                                                 acTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATACAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCt                    >  8:341561/1‑135 (MQ=255)                                                                                                                   tgctgcACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCtgg                  >  2:192700/1‑135 (MQ=255)                                                                                                                      tgcACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCtggtgg               >  8:228596/1‑135 (MQ=255)                                                                                                                      tgcACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCtggtg                <  6:133934/134‑1 (MQ=255)                                                                                                                      tgcACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCtggtg                <  6:302844/134‑1 (MQ=255)                                                                                                                        cACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGa              >  3:358873/1‑134 (MQ=255)                                                                                                                        cACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGa              >  2:215773/1‑134 (MQ=255)                                                                                                                        cACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTACAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGa              >  6:427761/1‑134 (MQ=255)                                                                                                                           cGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTg                                                                                      <  6:318788/59‑1 (MQ=255)                                                                                                                           cGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTg                                                                                      >  5:318788/1‑59 (MQ=255)                                                                                                                           cGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGaa          >  7:46106/1‑135 (MQ=255)                                                                                                                           cGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGaa          >  3:42033/1‑135 (MQ=255)                                                                                                                           cGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTCGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGaa          >  6:367718/1‑135 (MQ=255)                                                                                                                             ccTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAAtg        <  8:61493/135‑1 (MQ=255)                                                                                                                             ccTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAAtg        <  8:39493/135‑1 (MQ=255)                                                                                                                             ccTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAAtg        <  7:274069/135‑1 (MQ=255)                                                                                                                                gTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAAtgtcg     >  3:180995/1‑133 (MQ=255)                                                                                                                                gTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTgg     >  6:241569/1‑135 (MQ=255)                                                                                                                                gTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTgg     >  8:186918/1‑135 (MQ=255)                                                                                                                                gTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTgg     >  4:349896/1‑135 (MQ=255)                                                                                                                                gTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCGTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGAGGACGCATGTgg     >  5:40800/1‑135 (MQ=255)                                                                                                                                  aTCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTGGCg   <  4:378511/135‑1 (MQ=255)                                                                                                                                  aTCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTGGCg   <  4:358873/135‑1 (MQ=255)                                                                                                                                  aTCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGGGGACGAATGTGGCg   <  2:188181/135‑1 (MQ=255)                                                                                                                                   tCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTGGCgg  >  4:218772/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                    AACTGCCACGTGATCGCCAGCCGGATGATGAAGAGCGCGAAGACAGCTGGCAGCATGTGGTCGCCCTGGCCAAGACACTCAAGCCTGAAGAGTGGACAGAGGGCAACGAAACACTGCTGCACCGCCTGTATCACGAAGACGCCGTGCGCCTGTTCGATATCCAGCCACTGCGCTTCAATTGCAGCTGTTCGCGGGAACGCTCCGGCAATGCGCTGGTCAGTCTGGGCGAGCACGACGCCAAGGCGCTGGTGGACGAATGTGGCGG  >  ACB122/299654‑299918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑